http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 윤경하 (검색결과 65 건)
- 무료
- 기관 내 무료
- 유료
-
-
구름버섯(Coriolus versicolor) VK7 에 의한 Anthracene, Phenanthrene 및 Benzo(α)pyrene 의 분해
윤경하,최양순 순천향대학교 기초과학연구소 2001 순천향자연과학연구 논문집 Vol.7 No.1
The present research was undertaken to investigate the activity of ligninolytic enzymes and the biodegradation capability of anthracene, phenanthrene and benzo(α)pyrene with Coriolus versicolor VK7, isolated from nature and identified. For the assay of enzyme activities, the isolate did not produce lignin peroxidase (LiP) and veratryl alcohol oxidase (VAO), but laccase and manganese dependent peroxidase (MnP). While the activity for MnP was low (22.2 nmol/mgㆍprotein), its laccase activity was very high (740.0 nmol/mgㆍprotein). Moreover, laccase had appealed earlier than MnP. When the isolate was incubated with 10mg/L (final concentration) of anthracene, phenanthrene and benzo(α)pyrene, the isolate removed anthracene 74.1%, 86.1% and 100%, respectively for 7, 14 and 28 days incubation. In case of phenanthrene was 70.3%, 89.2% and 100%, respectively. Finally the results of benzo(α)pyrene was 58.3%, 64.8% and 84.8%, respectively. The resells suggest that laccase of Coriolus versicolor VK7 should play an important role in the biodegradation of these PAHs compounds.
-
-
구름버섯(Coriolus versicolor IFO 30388)에 의한 Poly R-478 염료의 탈색
윤경하,Yoon, Kyung-Ha 한국미생물학회 1994 미생물학회지 Vol.32 No.3
구름버섯에 의한 Poly R-478염료의 탈색에 미치는 탄소원과 질소원의 영향을 조사하였다. 2.0% glucose, 0.04% ammonium tartrate, 2% $KH_2PO_4$, 0.5% $MgSO_4{\cdot}7H_2O$, 0.1% $CaCl_2{\cdot}2H_2O$, 0.002% thiamine-HCI, 10 mM 2,2-dimethylsuccinate(sodium)와 0.02% Poly R-478 염료 등으로 조성된 질소제한배지에서 균주를 $28^{\circ}C$에서 10일간 정치배양 했을때 염료의 탈색율은 87.2%로 나타났다. 염료의 탈색은 배지내에 질소원이 존재할 때에 일어났으며 탈색율은 배지내에 질소원이 고갈된 직후에 급격히 증가하였다. Effects of nitrogen and carbon sources on the decolorization rate of poly R-478 dye by a white rot basidiomycete Coriorus versicolor IFO 30388 were examined. The fungus exhibited 87.2% of decolorization rate when it was cultured in the state of stationary in a nitrogen-limited medium (pH 4.5) which contained 2.0% glucose, 0.04% ammonium tartrate, 0.02% poly R-478 dye, 2% $KH_2PO_4$, 0.5% $MgSO_4{\cdot}7H_2O$, 0.1% $CaCl_2{\cdot}2H_2O$, 0.002% thiamine-HCl and 10 mM 2,2 dimethylsuccinate (sodium) at $28^{\circ}C$ for 10 days. Decolorization of the dye occurred in the presence of nitrogen source in the medium and decolorization rate increased rapidly after depletion of $NH_4^+$ from the medium.
-
Bacillus thuringiensis var israelensis의 돌연변이주에 관하여
윤경하,김영돈,심재철 한국곤충학회 1989 Korean journal of entomology Vol.19 No.3
B. thuringiensis var. israelensis를 N-methyl-N'-nitro-N-nitrosoguanidine으로 처리하여 4주의 돌연변이주를 분리하고 그들의 특성을 조사하였다. 균주 번호 s9는 아포를 형성하나 crystal을 형성치 못했고, s37은 아포를 형성치 못하고 crystal을 형성했다. 그리고 s194는 아포와 crystal를 형성하지 못한 반면 s295균주는 아포와 crystal을 모두 형성하면서 배지속에 짙은 갈색색소를 생성했다. 돌연변이주들이 생성하는 crystal의 모양은 둥글고 모균주의 crystal모양과 비슷했다. 돌연변이주들을 nutrient액체배지에서 28$^{\circ}C$로 진탕배양 했을 때 아포를 형성하는 균주들은 배양 24시간 이후에 최대의 생장을 나타냈고 아포를 형성치 못하는 균주들은 배양 36시간 이후에 최대의 생장을 나타냈다. Culex pipens 유충에 대한 치사효과 실험에서 spore-crystal복합체의 농도가 0.l $\mu\textrm{g}$/mL일때 s37 균주는 100%의 치사율을, s295균주와 모균주는 95%의 치사율을, 그리고 s9균주는 5%의 치사율을 나타냈다. 그러나 s194균주는 전연 치사효과를 나타내지 못했다. 아미노산 요구성, 항생제의 내성, 유화수소생성, 그리고 구연산염 이용여부실험등에서 돌연변이주들은 각기 다른 반응을 나타냈으나 arginine decarboxylase생성 실험에서는 아포를 형성하는 균주들은 활성을 나타냈고 아포를 형성하지 못하는 균주들은 활성을 나타내지 못했다. 본 연구의 결과들은 B. thuringiensis var. israelensis에서 crystal의 형성과 독성은 아포형성과 유전학적으로 무관하다는 것을 나타낸다. Four mutants were isolated from B. thuringiensis var. israelensis after N-methyl-N'-nitrosoguanidine (NTG) treatment. Of these mutants, s9 strain was sporogenic acrystalliferous, s37 strain asporogenic crystalliferous, s194 strain asporogenic acrystalliferous, and s295 strain sporogenic crystalliferous, producing dark brown pigment in culture media. Crystals produced by these mutants were spherical in shape and similar to those produced by the parent strain. When mutants were cultivated in the nutrient broth media at 28$^{\circ}C$ with rotary shaking, s9 and s295 strains were at a maximal growth after the 24 hours of incubation but s37 and s194 strains, after the 36 hours of incubation. In toxicity tests to the larvae of Culex pipiens, when they were exposed to 0.1 $\mu\textrm{g}$/mL of concentration of spore-crystal complex powder, while s37 strain showed 100% of lethal rate and s295 strain and parent strain 95% of lethal rate, with s9 strain showing 5% of lethal rate, s194 strain did not show a lethal effect on the other. In the tests of amino acid requirement, antibiotic resistance, sodium citrate and production of hydrogen sulfide, each mutant showed its different response and in the activity of arginine decarboxylase, asporogenous strains were negative, whereas sporogenous strain, positive. The results obtained in this study show that crystalliferous and toxic properties were genetically independent of sporulation in B. thuringiensis var. israelensis.
-
Dictyostelium discoideum의 포자가 발아중에 유출하는 protease에 관하여
윤경하,윤철호 한국미생물학회 1989 미생물학회지 Vol.27 No.1
Characteristics and roles of protease released during the germination of Dictyostelium discoideum spores were investigated. When geat activated, the spores germinated, progressively releasing the protease into the extracellular medium. The protease activity exhibited high at pH 2.5. When cyclogeximide was added to culture, complete germination (emergence) and protease release were stopped. Addition of purified nonspecific protease to culture speeded up germination. These results suggest that excreted protease may play a role in removal of the spore wall.
-
-
Doxylamine succinate 과량 복용 후 발생한 급성 신부전과 급성 췌장염 1예
윤경하,이창환,고정곤 의과학연구소 2000 全北醫大論文集 Vol.24 No.1
Anti-histamines are common over-the-counter medications which are frequently involved in overdoses. The usual clinical course is dominated by the anti-cholinergic effects of these agents; it includes significant autonomic and central nervous system effects and direct cardiac toxicity. We report a case of a suicide at-tempt in a young adult male wher ingestion of a lot of doxylamine was complicated by acute pancreatitis and acute renal failure. (Key words : antihistamine, acute pancreatitis, acute renal failure)
-
한국산 고온성 방선균의 Protease 생산에 관한 연구
윤경하 순천향 대학교 1984 논문집 Vol.7 No.2
우리 나라 중부 이남에서 수집한 180여개의 표품으로부터 90주의 고온성 방선균을 분리하고 이들의 단백질 분해효소의 활성을 측정 비교하여 효소생산능력이 우수한 균주를 선정함과 동시에 그의 단백질분해효소의 생산을 위한 몇가지 배양조건을 조사하였다. (1) 단백질분해효소의 생산능에 있어서 우수균주로 선정된 S68-2는 산성, 중성 및 알카리성 단백질분해효소의 활성을 11.0, 17.4, 그리고 17.3으로 각각 나타냈다. (2) 알카리성 단백질분해효소의 생산은 배지의 조성이 3.0% soluble starch, 1.0% peptone, 0.05% MgSO₄·0.15% K₂HPO₄, 0.002% FeSO₄·7H₂O 0.01% CaCl₂·일 때 가장 양호하였다. (3) 효소생산의 최적배양조건하에서 24시간 진탕배양 했을 때 단백질분해효소의 활성(21.5㎍ /ml)이 가장 높았다. Protease activities of thermophilic Actinomycetes which were isolated from various habitat substrates collected througout South Korea were measured. The cultural conditions of the best strain in protease production were investigated. 1. Among the 90 strains of thermophilic Actinomycetes isolated, strain number S68-2 was selected as the predominant strain in acidic, neutral and alkaline protease activity. 2. The composition of effective medium for alkaline protease production by S68-2 strain was 3.0% soluble starch, 1.0% peptone, 0.05% MgSO₄·7H₂O, 0.15% K₂HPO₄, 0.01% CaCl₂·2H₂O and 0.002% FeSO₄·7H₂O. 3. S68-2 strain exhibited the most remarkable protease activity when it was grown at 50℃ for 24 hours with rotatory shaking.
-
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
