骨隨細胞에서의 染色體 分折法에 대한 硏究

김태전 서울保健大學 1987 論文集 Vol.7 No.1

강원도 일부 지역주민들에서의 간염 B항원 발현율에 관한 조사

김태전,문희주 최신의학사 1979 最新醫學 Vol.22 No.12

This study was done to report HBs antigen positive rate to a part of inhabitants at Kang Woon Do, and compare between the urban and the rural the occurrence rate of HBs antigen. The following results were obstained in this study ; 1)In the rural, HBs antigen positive rate was 7.3%, and it was not significantly higher than urban. 2)In sex and ages; HBs antigen positive rate generally indicated higher trend in male than female, in younger than older people. 3)Especially, preschool children was statistically the highest than student group and adult group in HBs antigen positive rate (P<0.01). 4)The relationship of blood type and HBs antigen positive rate was not significant statistically. In the above results, there could see that the infection of hepatitis B virus had a very close relationship to unsanitary environment, low economic and cultural level. Especially there should consider a healthy countermeasure for preschool and primary school children in rural area.

토양에서 분리된 방선균들에 의한 Rimazol Brilliant Blue R(RBBR) 염료의 탈색에 질소원과 pH가 미치는 영향

김태전,김승곤,윤경하 서울保健大學 1997 論文集 Vol.17 No.1

Many workers had reported that the degradation of lignocellulose and lignin by white rot fungi were significantily correlative with the degradation of phenol or PAHs (Polycyclic aromatic hydorcarbons) compounds and the decolorization of a various dyes, specially RBBR (Remazol brilliant blue R) and Poly R-478 dyes. but it were not known distinctly that the degradation of these and the decolorization of these by Actinomycetes were related to the degradation of phenol or PAHs compounds, and author had examined in relationship between the degradation of lignocellulose or lignin and the decolorization of RBBR by Actinomycetes isolated in 67 soil samples collected at 8 areas of Korea from February, 1994 to March, 1995. In the experiment, There were selected two strains identified as Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 which had good correlation between the degradation of lignocellulose or lignin, and it were investigated that the cultural parameters to maximize the decolorization ratios of RBBR by two strains, as the results, Streptomyces halstedii scabies SA1-36 revealed each the highest decolorization ratios with 73.4% in 0.30% glycerin and Streptomyces lavendulas SA2-14, with 74.1% in 0.25% cellobiose at 30℃ in 20 days of culture. There were used their results as basal data in this study that examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR. It were summarized as following that the results examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR by two strains. 1) It were the highest each as 75.7% in casein and 75.7% in yeast extract that the dicolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to nitrogen sources(0.l%). 2) It were the highest each as 76.8% in 0.1% casein and 78.3% in yeast extract that the decolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to the concentrations of casein and yeast extract. 3) It were the highest each as 76.8% in pH 7.6 and 79.4% in pH 6.6 that the decolorization ratios of RBBR according to pH by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains. In the above results, we could knowe that it were each different as well as carbon sources that nitrogen sources and pH required between Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2- 14 strains. and organic nitrogens influence more than inorganic nitrogens to decolorize RBBR dye and nitrogen sources and pH influence lower than carbon sources to decolorize RBBR dye. What`s more, we could knowe that it were very important to use newly formuliged optimal basal media maximize decolorization ratios of RBBR dye by Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2-14 strains.

江原道 一部地域住民들에서 肝炎B 抗原 陽性率에 관한 調査

金泰田 서울保健專門大學 1982 서울保健專門大學 論文集 Vol.2 No.-

일부 헌혈자들에 대한 실태조사

金泰田 대한임상병리사협회 1979 임상병리사회지 Vol.11 No.1

임상검체에 분리된 Serratia sp. 2000-1에 의한 Protease의 생산 및 효소학적 성질

김태전,김승곤,김상택 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.3

본 논문은 환자들의 병소에서 채취된 가검물에 존재하는 미생물들 가운데 protease을 분비하는 균주들을 분리하고, 그 가운데 가장 효소생산력이 우수한 균주를 선정하여 가정용 Protease 세제생산에 활용 가능성을 알아보고자 그들이 생산하는 pretense의 기초적 생산조건 및 부분적 효소학적 특성 등에 관한 실험에서 얻은 결과들을 보고하고자 작성되었다. Serratia sp. 2000-1로 동정된 본 실험 균주가 생산하는 protense의 기초적 생산조건과 부분적 효소학적 특성을 조사한 결과 효소생산을 위한 최적 배지에 탄소원과 질소원 그리고 금속염의 종류와 농도는 각각 Glucose 1.5% C.S.P 2.0% Cacl2 0.1%였다. 그리고 최적 배양온도는 30℃, 초발 pH는 8.0, 배양시간은 72시간 이었다. Protease의 정제는 ammonium sulfate precipitation, DEAE-cellulose, Sephadex G-200 column을 통하여 분리 정제하였으며 이때 최종 효소수율은 14.4%, 효소 비활성도는 약 29배 증가한 것으로 나타났다, 그리고 효소 작용의 최적온도와 pH는 35℃와 pH 8.0으로 나타났고, 효소 작용의 열과 pH에 대한 안정성은 40℃와 pH 6∼10까지는 효소의 활성이 비교적 안정한 것으로 나타났다. 금속이온들 중 Mg2+, Ba2+, Ca2+, Mn2+ 은 효소활성을 촉진하나 Hg2+, Ag2+, Cu2+는 효소활성을 저해하는 것으로 나타났다. 그리고 효소활성 저해제들 중에는 SDS가 가장 강하게 효소활성을 저해하는 것으로 나타났다. The purpose of this study was to investigate the practical availability of protease production that can be used at home after isolating Serratia sp. 2000-1 which produced extracellular pretease from clinical specimen. Basic production conditions and partial enzymatic characteristics of pretease produced by Serratia sp. 2000-1 was as follows: The kind and concentration of carbohydrate, nitrogen and metal salts fur optimal enzyme production condition were each identified as the concentration of 1.5% glucose, 2.0% CSP, and 0.1% Cacl2, and the optimal temperature, time and initial pH for culture were each 30℃, 72 hours, and pH 8.0. The final enzymatic yeild that was purified by 3 steps with ammonium sulfate precipitation (45∼80%), DEAE-cellulose column chromatography, and Sephadex G-200 gel chromatography was 14.4%, and enzyme inactivity rate increased approximately 29 folds. The optimal temperature and pH for purified protease activity were 35℃ and pH 7.0∼8.0, and purified pretense activity was relatively stable by 40℃ at pH 6∼10 for 30 min, however heating at 60℃ for 30 min, it liminated detectable pretease activity. The protease activity was activated by Mg2+, Ba2+, Ca2+, Mn2+, but inactiviaed by Hg2+, Ag+, Cu2+, and the protease activity was inhibited strongly by SDS among enzyme activity inhibitors. Further study is required to evaluate the practical availability of pretease production that can be used at home by isolating Serratia sp. from more clinical specimen and examining protease more in details.

한국 토양에서의 방선균 분리와 PAH화합물의 분해능에 관한 연구 : Rimazol Brillant Blue R염료의 탈색을 중심으로

김태전 서울保健大學 1995 論文集 Vol.15 No.1

Decolorization of remazol brilliant blue R(RBBR)dye, which can take the place of the degradation of polycyclic aromatic hydorcarbons(PAHS) by actinomycetes isolated in 67 soil samples collected at 8 areas of Korea, and parameters that may influence decolorization were examined from February, 1994 to March, 1995. The results were summarized as following. ① Each actinomycetes isolated on Al medium, A2 medium and A3 medium from 67 soil samples were 822 strains, 763 strains, and 783 strains. therefore Al selective medium was very good for isolation of actinomycetes ② The optimal pH of soil samples for growth of actinomycetes was 7.0∼7.5. ③ The optimal temperature for degradation of lignocellulose, lignin, and decoloization of RBBR(0.005%)dye was 30℃. ④ Strains screened in A1S medium for the degradation of lignocellulose were 205 strains among 822 strains isolated in Al medium, strains screened in M3 IND medium for degradation of lignin were 108 strains among 205 strains, and 108 strains were identified finally with one strain of amycolata autotrophica and 13 strains of streptomyces. Strains indicated decolorization ratios over 50% by agar plug and standing culture were 17 strains among 108 strains, and strains showed decolorization ratios over 60% by cell suspension (O.D; 0.85, inoculation volume: 0.2ml) and shaking culture were 12 strains among 17 strains. ⑤ Decolorization ratios of 12 strains according to carbon sources(0.2%) were very good in glycerin with 73.2% to streptomyces halstedii scabies SAl-36 and in cellobiose with 73.1% to streptomyces lavendulas SA2-14. ⑥ Decolorization ratios according to the concentrations of cellobiose to streptomyces lavendulas SA2-14 and of glycerin to streptomyces halstedii scabies SAl-36 were the most high ratios with 74.1% in 0.25% cellobiose and with 73.4% in 0.25% glycerin. In the above results, it will be possible in the future to screen actinomycetes which can degrade PAH compounds included in natural, industrial wastes and stain solution discharged during to stain tissue preparations through decolorization abillity of RBBR dye by actinomycetes.

一部 公務員에서의 HBsAg의 發現率과 生化學的 檢査値에 關한 調査

김태전,권혁한 서울保健大學 1983 論文集 Vol.3 No.1

임상검체에서 분리된 Serratia sp. 2000-1에 의한 Pretense의 생산 및 효소학적 성질

김태전,김승곤,김상택 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.3

본 논문은 환자들의 병소에서 채취된 가검물에 존재하는 미생물들 가운데 pretense을 분비하는 균주들을 분리하고, 그 가운데 가장 효소생산력이 우수한 균주를 선정하여 가정용 Protease 세제생산에 활용 가능성을 알아보고자 그들이 생산하는 protease의 기초적 생산조건 및 부분적 효소학적 특성 등에 관한 실험에서 얻은 결과들을 보고하고자 작성되었다. Serratia sp. 2000-1로 동정된 본 실험 균주가 생산하는 protease의 기초적 생산조건과 부분적 효소학적 특성을 조사한 결과 효소생산을 위한 최적 배지에 탄소원과 질소원 그리고 금속염의 종류와 농도는 각각 Glucose 1.5%, C.S.P 2.0%, CaCl$_2$ 0.1%였다. 그리고 최적 배양온도는 3$0^{\circ}C$, 초발 pH는 8.0, 배양시간은 72시간 이었다. Protease의 정제는 ammonium sulfate precipitation, DEAE-cellulose, Sephadex G-200 column을 통하여 분리 정제하였으며 이때 최종 효소수율은 14.4%, 효소 비활성도는 약 29배 증가한 것으로 나타났다. 그리고 효소 작용의 최적온도와 pH는 35$^{\circ}C$와 pH 8.0으로 나타났고, 효소 작용의 열과 pH에 대한 안정성은 4$0^{\circ}C$와 pH 6~10까지는 효소의 활성이 비교적 안정한 것으로 나타났다. 금속이온들 중 $Mg^{2+}$, $Ba^{2+}$, $Ca^{2+}$, Mn$^{2+}$은 효소활성을 촉진하나 Hg$^{2+}$, Ag$^{2+}$, Cu$^{2+}$는 효소활성을 저해하는 것으로 나타났다. 그리고 효소활성 저해제들 중에는 SDS가 가장 강하게 효소활성을 저해하는 것으로 나타났다. The purpose of this study was to investigate the practical availability of pretense production that can be used at home after isolating Serratia sp.2000-1 which produced extracellular pretense from clinical specimen. Basic production conditions and partial enzymatic characteristics of pretense produced by Serratia sp. 2000-1 was as follows: The kind and concentration of carbohydrate, nitrogen and metal salts for optimal enzyme production condition were each identified as the concentration of 1.5% glucose, 2.0% CSP, and 0.1% CaCl$_2$, and the optimal temperature, time and initial pH for culture were each 3$0^{\circ}C$, 72 hours, and pH 8.0. The final enzymatic yeild that was purified by 3 steps with ammonium sulfate precipitation (45~80%), DEAE-cellulose column chromatography, and Sephadex G-200 gel chromatography was 14.4%, and enzyme inactivity rate increased approximately 291314s. The optimal temperature and pH for purified pretense activity were 35$^{\circ}C$ and pH 7.0~8.0, and purified pretense activity was relatively stable by 4$0^{\circ}C$ at pH 6~10 for 30 min, however heating at 6$0^{\circ}C$ for 30 min, it liminated detectable pretense activity. The pretense activity was activated by $Mg^{2+}$, $Ba^{2+}$, $Ca^{2+}$, Mn$^{2+}$, but inactiviaed by Hg$^{2+}$, Ag$^{2+}$, Cu$^{2+}$, and the pretense activity was inhibited strongly by SDS among enzyme activity inhibitors. Further study is required to evaluate the practical availability of pretense production that can be used at home by isolating Serratia sp. from more clinical specimen and examining pretense more in details.

갑상선의 정상과 여포상 종양 조직의 은 핵소체 형성부위(AgNOR)와 증식세포 핵 항원(PCNA)에 관한 조사

김태전,한경희 임상병리검사과학회 2001 임상병리검사과학회지 Vol.33 No.2

The histologic or cytologic distinctions between follicular adenoma and carcinoma in thyroid tissue are sometimes difficult. For the diagnosis of follicular carcinoma, invasion of blood vesseles or capsules are considered definitive criteria. However, the distinction of follicular adenoma from carcinoma may be difficult when these features are absent. Furthermore, in case with fine needle aspiration biopsy specimen, the distinction between follicular adenoma and carcinoma may also be difficult, as both are characterized by follicular form cells. The authors performed the sliver nucleolar organizer region(AgNOR) technique and immunohistochemistry for proliferating cell nuclear antigen(PCNA) on 5 samples of normal thyroid tissue, 8 follicular adenomas, and 10 carcinomas to evaluate whether these techniques can aid in these differential diagnosis. Significant differences were found in the mean AgNOR count between normal thyroid tissue (1.29±0.65) and follicular adenomas (2.88±1.33) (P<0.000l), but not between follicular adenomas and carcinomas(2.71±1.14). In the normal thyroid tissue, AgNORs were smooth and round; in the follicular adenomas and carcinomas, however, they varied in size and shape. Compound AgNORs were commonly present in the tumors but were absent in normals. Antibody to PCNA did not stain normal thyroid tissue cells but stained many follicular adenomas and carcinoma cells. These results suggested that the AgNOR technique and PCNA immunohistochemistry could be used to distinguish normal thyroid tissue form follicular adenoma, but not distinguish follicular adenoma from carcinoma.