Detection of Puumala and Hantaan Virus among Bats in Korea by Nested RT - PCR

Yun Tai Lee,Bo Kyoung Yun,Jeong Joong Yoon 대한바이러스학회 1998 Journal of Bacteriology and Virology Vol.28 No.2

Hantavirus is a genus of the Bunyaviridae family consisting following serotype groups: Hantaan, Seoul, Puumala, Prospect Hill, Thailand, Belgrade, Thotta palayam, Sin Nombre. Most of Hantavirus group have been associated with many clinically similar disease known collectively as hemorrhagic fever with renal syndrome (HFRS). Hantaan virus is the prototype of the genus hantavirus, originally isolated from Apodemus agrarius. Bat was found as a natural host for Hantaan virus in Lee's lab for the first time. Then, Hantaan-like virus was isolated Hantaan-like virus from bat. To identify hantaviruses that are present in Korea among bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area, RNA was isolated from lung and serum. RT-PCR was performed with a universal primer from M segment. Nested RT- PCR was carried out to differentiate Hantaan, Seoul and Puumala virus using serotype specific primers. As we expected, Hantaan viruses were detected in bats and Seoul virus was not detected. Interestingly, Puumala viruses were also detected in bats from Won-Ju, but not in other areas. Puumala virus is originally isolated from Clethrinomys glareolus, and cause light HFRS. Recently, Paradoxomis webbiana, a wild bird turn out to be a reservoir for Puumala virus in Korea. These data indicate that bat is a new natural reservoir of Puumala virus.

Detection of Puumala and Hantaan Viruses among Bats in Korea by Nested RT-PCR

Lee, Yun-Tai,Yun, Bo-Kyoung,Yoon, Jeong-Joong The Korean Society of Virology 1998 Journal of Bacteriology and Virology Vol.28 No.2

Hantavirus is a genus of the Bunyaviridae family consisting following serotype groups: Hantaan, Seoul, Puumala, Prospect Hill, Thailand, Belgrade, Thotta palayam, Sin Nombre. Most of Hantavirus group have been associated with many clinically similar disease known collectively as hemorrhagic fever with renal syndrome (HFRS). Hantaan virus is the prototype of the genus hantavirus, originally isolated from Apodemus agrarius. Bat was found as a natural host for Hantaan virus in Lee's lab for the first time. Then, Hantaan-like virus was isolated Hantaan-like virus from bat. To identify hantaviruses that are present in Korea among bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area, RNA was isolated from lung and serum. RT-PCR was performed with a universal primer from M segment. Nested RT-PCR was carried out to differentiate Hantaan, Seoul and Puumala virus using serotype specific primers. As we expected, Hantaan viruses were detected in bats and Seoul virus was not detected. Interestingly, Puumala viruses were also detected in bats from Won-Ju, but not in other areas. Puumala virus is originally isolated from Clethrinomys glareolus, and cause light HFRS. Recently, Paradoxomis webbiana, a wild bird turn out to be a reservoir for Puumala virus in Korea. These data indicate that bat is a new natural reservoir of Puumala virus.

강력범죄 피해자의 외상 후 스트레스 증상 및예측요인에 대한 탐색적 연구

김태경(Kim, Tae­Kyoung),윤성우(Yun, Sung­Woo),이영은(Lee, Young­Eun),이새롬(Lee, Saerom) 한국피해자학회 2018 被害者學硏究 Vol.26 No.1

The aim of this study was to investigate the posttraumatic stress symptoms of violent crime and predictors to the symptoms. For this, we analyzed data of 473 victims registered to the integrated supporting center for violent crime victims who agreed to participate in the study. The results showed that more than 90% of victims experience significant PTSD symptoms, 30% of them were in extreme mental confusion that required medication or hospitalization. There was a significant difference in the severity of sequela according to gender, time since visiting to the center, pre-event adaptation level, and event type. Although the victim experienced more avoidance and hypervigilance symptoms than the family, the psychological distress experienced by the family was not less than that of the victim. Psychological sequela of the events were found to be more severe with more experiences of childhood trauma, lack of ego resilience, low level of perceived social support, strong dissociation tendency, and avoiding coping styles. A result of the stepwise regression analysis, avoidant coping style explained 10.6% of the total variance. Limitations and implications of this study were discussed.

ATHB12, an ABA-lnducible Homeodomain-Leucine Zipper (HD-Zip) Protein of Arabidopsis, Negatively Regulates the Growth of the Inflorescence Stem by Decreasing the Expression of a Gibberellin 20-Oxidase Gene

Ora Son,허윤선,김윤경,이현정,김성한,김미란,남경희,이명석,김부용,박종범,박정안,이석찬,Atsushi Hanada,Shinjiro Yamaguchi,이인정,김승기,윤대진,Eva So¨derman,천충일 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

ATHB12, an ABA-lnducible Homeodomain-Leucine Zipper (HD-Zip) Protein of Arabidopsis, Negatively Regulates the Growth of the Inflorescence Stem by Decreasing the Expression of a Gibberellin 20-Oxidase Gene

Ora Son,허윤선,김윤경,이현정,김성한,김미란,남경희,이명석,김부용,박종범,박정안,이석찬,Atsushi Hanada,Shinjiro Yamaguchi,이인정,김승기,윤대진,Eva So¨derman,천충일 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

억제대 적용지침 개발을 통한 불필요한 억제대 적용율 감소활동

곽경선,김성은,배은경,이은숙,이은혜,최윤정,이윤경 한국의료QA학회 2009 한국의료질향상학회지 Vol.15 No.2

문제: 억제대 적용에 대한구체적 적용지침 없이 의례적으로 억제대를사용함으로 인해 환자들의 신체적 또는 정신적 안전을 위협할 수 있다. 목적: 완전 진정상태 이거나 근력이 약하여 발관능력이 없는 환자에 대한 예방적 적용, 바빠서 환자를볼수 없 는경우, 간호사본인의 판단이 없이 타인의 요구에 따르는등의 불필요한 억제대 적용이 증가하는 원인을 파악하여 중환자실의 특수성에 맞는 억제대 적용및 제거 지침을 마련하고자 한다. 의료기관: 인천광역시에 소재한 의과대학 소속병원의 집중치료실 개선효과: 억제대 적용에 대한중환자실 간호사에게 미치는 영향을 보면 태도 정도 p=0.09(p<0.1), 올바른 수행 능력은 p= 0.005 (p <0.0히로통계적으로유의한것으로 나타났다. 지식 정도 p=0.172(p<0.05) 통계적으로 유의 하지는 않지만 모든항목이 개선 활동전 에 비해 2,3차 개선 활동후에 향상한 것으로 나타났다. 개선활동전에 8.1%였던 간호 기록, 0.7%였던 간호중재, 0%였던 의사처방율이 3차 개선 활동 후 3가지 모두 100% 달성되었다. 이는 억제대 적용율 갑소 및 부적절한 억제대 적용율은 유의하게 감소하였다. 교훈: 중환자실 간호사의 억제대 적용에 대한태도, 지식, 올바른 간호 수행 능력은 지속적으로 재평가 되어지고, 교육내용은좀더 나은 간호제공을 위하여 개발 되어져야 할 것이다. 억제대 적용 기준과 제거 기준을 Check List 하여 객관성의 유지를 위한 개선 활동은계속적으로 Feedback 되어야 할 것이다.

마우스에서 고려홍삼 추출물의 단회 및 반복투여독성 평가

임숙희, 신선희, 장자영, 변상국, 이예은, 박동선, 전정희, 이용경, 이동만, 이세라, 김재승, 황방연, 노재섭, 남상윤, 윤영원, 김윤배 충북대학교 동물의학연구소 2005 Journal of Biomedical and Translational Research Vol.6 No.2

본문참조

지역사회 기혼 여성의 우울 증상에 영향을 미치는 변인 : 가족내 관계 만족 변인 및 갈등대처 방식을 중심으로

이경미,최윤경,강이헌,이민수 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.2

연구목적 본 연구는 지역사회 기혼 여성의 우울 증상을 사회적이고 대인관계적 측면에서 이해하고자 하는 맥락에서 살펴보고자 하였다. 방법 00시에 거주하는 기혼여성 632명을 대상으로 하여 Beck 우울증 척도, 결혼 전반적 불만족 척도, 자녀불만족 척도, 갈등 대처 방안 척도 등을 실시하였다. 통계방법으로는 기술통계분석, 상관, χ²검증, 일원변량분석, 단계적 중다회귀분석, 위계적 회귀분석을 사용하였다. 결과 학력, 수입, 부모의 관계, 학대경험, 시부모 관계만족, 결혼불만족, 자녀불만족, 이성적 대처, 감정 · 행동표출, 회피 행동에서 우울수준에 따른 집단간 차이가 유의미하였다. 또한 이들 변인 중 결혼불만족이 현재 우울증상에 대한 설명력이 가장 큰 것으로 나타났고, 다음으로 자녀불만족, 회피 행동, 시부모 관계만족 순이었다(총설명변량=36.1%). 갈등대처방식 중 이성적 대처와 회피가 가족관계 만족변인과 우울을 잠정적이지만 유의미하게 매개하는 것으로 나타났다. 결론 지역사회 기혼 여성의 상당수가 우울 증상을 경험하고 있어 이에 대한 치료적 개입이 시급하며 특히 부부 관계를 포함한 가족 관계에 대한 탐색 및 접근이 필요한 것으로 나타났다. 특히 갈등대처방식이 유의미하게 관계 만족과 우울을 매개하므로, 우울증의 치료에 있어 효과적인 갈등 대처 방식 및 사회적 문제 해결 기술을 포함시킴으로써 치료 효과를 높일 수 있을 것으로 시사된다. Objectives : The purposes of this study were to investigate depressive symptoms among married women and to their Understand depression through social and interpersonal factors. Methods : The subjects were consisted of 632 married women who lived in 00 city. They completed the Beck Depression Inventory, Marital Global Distress Scale, Dissatisfaction with Children Scale, and Conflict-coping Method Scale. Descriptive statistics, correlation, χ²-test, ANOVA, step-wise regression, and hierarchical regression were applied to analyze the data. Results : ANOVA analysis showed the differences in education, income, experience of abuse, parent-in-law relationship, marital global distress, dissatisfaction with children, rational coping, emotion/action-expression coping, and avoidant coping among the non-depressive group, mildly depressive group, and depressive group. Step-wise regression showed that the variance of the current depressive symptoms was accounted for by marital global distress, dissatisfaction with children, avoidance coping method, and satisfaction with parent-in-law (total account=36.1%). It was found that the rational coping method and avoidance coping method significantly mediated the relationship between the satisfaction with family relation and depressive symptoms. Conclusion : Therapeutic interventions for the married women experiencing depressive symptoms showed include their marital relationship. Based on the finding that the conflict coping methods mediated the association between the depressive symptoms and the family relation satisfaction, the effective conflict coping skills or social problem solving skills should be included in the interventions for married women.

시판용 상토 종류가 배추 유묘기 묘소질에 미치는 영향

이상우,강호종,안상열,채윤석,윤재길,최경옥 진주산업대학교 농업기술연구소 2007 農業技術硏究所報 Vol.20 No.-

시중에 판매되고 있는 상토는 제조 회사에 따라 재료의 구성 및 혼합 비율이 다르다. 시판용상토를 제조사별로 구입하여 상토 제조사에 따라 배추 육묘기 묘소질에 미치는 영향에 대해 조사하였다. 상토 제조사 별로 배추의 지상부 및 지하부의 생육에 많은 차이가 있었다. 그리고 엽록소 함량 및 단백질 함량에도 차이가 많았으며, 엽록소 및 단백질 함량이 높았던 상토에서 배추의 생육이 좋았다. 상토 제조사별로 상토의 재료 및 혼합비율에 따라 배추의 유묘기 생육에 많은 영향을 미치는 것으로 나타났다. The substrate is very important to grow seedling because of directly effect on seedling quality of Chinese cabbage. The substrates manufactured by the companies consist of different materials and mixing ratios. This study was conducted to observe the seedling quality of Chinese cabbage by using of the kinds of substrate. The number of leaf, leaf area, fresh weight, dry weight, root length, root weight and T/R ratio of the seedling were shown in different from each substrate manufactured by the companies. And chlorophyll and protein content of the seedling were also shown in different from substrates supplied from the companies, respectively. Substrate which was high in that was also higher in seedling quality. The material and mixing ratios of substrate effected on the growth of cabbage

석면이 흰쥐폐의 섬유모세포 증식에 미치는 영향

이동원,김경아,임 영,윤임중 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.3

Asbestosis is chronic inflammatory disorder of lower respiratory tract in which alveolar wall are progressively thickened by a fibrotic process. Fibrotic process characterized by an expansion of fibroblast and collagenous extracellular matrix secreted from this fibroblast. Alveolar macrophage is believed to be a primary target cell and major participant in the evolution of lung fibrosis after asbestos inhalation. Alveolar macrophage are known to release a variety of substance that induce tissue damage and stimulate inflammatory cells and fibroblast. Macrophage also release a variety of metabolite of arachidonic acid. Of these, PGE₂is known to suppress fibroblast proliferation. Asbestos may be a very effective stimulus for fibroblasts without triggering the release of PGE₂. To assess the fibrogenic properties of asbestos according to kind and dosage of asbestos and the ability of PGE₂to suppress the proliferation of fibroblast, alveolar macrophages cultured with crocidolite, amosite and chrysotile in presence or absence of PGE₂10-5M. At 24 hours after alveolar macrophage cultured with various stimuli, the released fibronectin and TNF-α was measured. Viability of alveolar macrophages was observed and growth promoting activity of macrophage supernatant to fibroblasts was quantified. The results were as follows; 1. The viability of alveolar macrophages stimulated with asbestos fiber was markedly decreased compared with control group except chrysotile 10 μg group. Crocidolite and amosite were more cytotoxic than chrysotile. 2. All of asbestos augmented fibronectin production in concentration dependent fashion. 3. There was a significant positive correlation between TNF-α production in supernatant and fiber concentration. 4. Supernatant from alveolar macrophages cultured with asbestos were inducible a significant increase in fibroblast proliferation. 5. Incubation of alveolar macrophages with asbestos in the presence of PGE₂resulted in significant decrease of TNF-α production in supernant. 6. Supernatant from alveolar macrophages cultured with asbestos were inducible a significant decrease in fibroblast proliferation when PGE₂was added. The result of this study strongly suggested that crocidolite and amosite were more cytotoxic and fibrogenic and exogenous PGE₂suppressed fibroblast proliferation following exposed to asbestos.