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애기장대 줄기 조직배양에 있어서 식물생장조절제가 캘러스 형성과 기관분화에 미치는 영향
박정안,박종범,Park, Jung-An,Park, Jong-Bum 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.3
This experiment was carried out to investigate the effects of plant growth regulators on the organogenesis from the tissue culture of Arabidopsis thaliana stem, and the origin of the callus development. When the stem segments were cultured on medium with 2mg/L IAA or NAA, adventitious roots and trichomes were differentiated after 11 days of culture. Callus vigorously formed on medium with 2/L2,4 after 7 days of culture, but adventitious roots and trichomes were not differentiated from callus after 10 days of culture. This results suggesting that picloram is very effective auxin for the callus formation and organogenesis. Callus weakly formed on 0.05mg/L kinetin, and formed on combination of auxins(2mg/L) with 0.05mg/L kinetin. But the effect of combination of auxins and kinetin the callus formation was less than 2,4-D or picloram alone. A histological examination of callus formed on picloram showed that phloram showed that phloem parenchyma cells were divided and enlarged after 2 days of culture. Cortex parenchyma cells were divided and meristematic nodules were developed from these cells after 4 days of culture. Finally, callus formed on outside of cortex and epidermis by division of meristematic nodules after 7 days of culture.
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박정안,S.M. Hemayet Jahan,Woo-Geun Song,Hyejung Lee,Young-Su Lee,Hong-Soo Choi,Kwan-Suk Lee,Chang-Suk Kim,이석찬,이경열 한국응용곤충학회 2012 Journal of Asia-Pacific Entomology Vol.15 No.1
Bemisia tabaci is a species complex that consists of at least 24 genetically diverse biotypes. Here, we determined the biotypes of 27 populations collected in 17 different regions of Korea. Nucleotide sequence comparisons of cytochrome oxidase showed that 26 populations were Q biotype and that one population, the Goyang population, was B biotype. Further subgroup analysis of the Q biotype showed that all populations belonged to the Q1 subgroup, which originates from Western Mediterranean countries. Five endosymbiotic bacteria from various B. tabaci populations were analyzed by comparing rDNA sequences. Hamiltonella was detected in all the populations tested regardless of biotype. Cardinium was detected in all Q biotype populations but not in the B biotype population, while Rickettsia was detected in the B biotype population but not in Q biotype populations. Arsenophonus and Wolbachia were detected in 35% and 58% of Q biotype populations, respectively,but not in the B biotype population. Our results show that the endosymbiont profile is strongly associated with each biotype and with subgroups of the Q biotype. Survey of TYLCV disease from 2008 to 2010 indicated that this disease is widely spread in Korea. This study suggests that the rapid spread of TYLCV may be associated with endosymbiont infection, particularly Hamiltonella infection of B. tabaci.
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토양 서식 미생물을 이용한 가축사체 매몰지 토양유래 용존 유기물 분해
박정안(Jeong Ann Park),강진규(Jin Kyu Kang),김재현(Jae Hyun Kim),김성배(Song Bae Kim) 大韓環境工學會 2013 대한환경공학회지 Vol.35 No.12
본 연구의 목적은 가축사체 매몰지 토양에서 추출된 미생물을 이용하여, 가축사체 매몰지 오염 토양 유래 유기탄소의 분해 효과를 분석하고, 분해에 관여하는 미생물의 종을 규명하는 것이다. 경기도 안성에 위치한 가축 매몰지 토양 유래 용존 유기탄소를 대상으로 토양 서식 미생물의 생분해율을 평가한 결과(56일 동안), 1번 용액(초기 용존 유기탄소 농도 = 19.88 mg C/L)에서는 13일 이내에 48%의 용존 유기탄소가 감소하였으며, 56일째 용존 유기탄소는 8.8 ± 0.4 mg C/L로 56% 분해되었다. 2번 용액(초기 용존 유기탄소 농도 = 19.80 mg C/L)에서도 초기 13일 이내에 용존 유기탄소 농도가 급격히 감소하였으며, 56 일째 용존 유기탄소는 6.0 ± 1.6 mg C/L로 70%가 분해되었다. 생분해 실험과정에서, 전체 유기탄소 물질에서 방향족 탄소구 조의 비율을 나타낸 지표인 SUVA₂54값은 용존 유기탄소와는 다르게, 일정기간(21일) 동안 증가하다가 감소하는 경향을 나타 냈다. SUVA₂54값은 1번 용액과 2번 용액 모두, 21일째 가장 높은 값을 나타내었다. 생분해 실험 14일째 미생물 분석 결과, 토양 에서 쉽게 발견되는 Pseudomonas fluorescens, Achromobacter xylosoxidans, Nocardioides simplex, Pseudomonas mandelii, Bosea sp. 등 미생물이 검출되었다. 그리고, 56일째 미생물 분석 결과, Pseudomonas veronii가 우점종으로 나타났다. The aim of this study was to investigate the biodegradation of dissolved organic matter derived from animal carcass disposal soil using soil inhabited bacteria and to identify the bacteria involved in the biodegradation. The two soils were obtained from the animal carcass burial sites located in Anseong, Gyeonggi-do, Korea. The results indicated that during the biodegradation experiments (56 days), 48% of dissolved organic carbon (DOC) was mineralized within 13 days in soil-derived solution 1 (initial DOC = 19.88 mgC/L), and the DOC concentration at 56 days was 8.8 ± 0.4 mg C/L, indicating 56% mineralization of DOC. In soil-derived solution 2 (initial DOC = 19.80 mgC/L), DOC was mineralized drastically within 13 days, and the DOC concentration was decreased to 6.0 ± 0.4 mg C/L at 56 days (76% mineralization of DOC). Unlike DOC value, the specific UV absorbance (SUVA₂54) value, an indicator of proportion of aromatic structures in total organic carbon, tended to increase until 21 days and then decreased thereafter. The SUVA₂54 values in soil-derived solutions 1 and 2 were the highest at 21 days. The microbial analysis demonstrated that Pseudomonas fluorescens, Achromobacter xylosoxidans, Nocardioides simplex, Pseudomonas mandelii, Bosea sp. were detected at 14 days of incubation, whereas Pseudomonas veronii appeared as a dominant species at 56 days.
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박정안(Jeong Ann Park),최낙철(Nag Choul Choi),김성배(Song Bae Kim) 大韓環境工學會 2013 대한환경공학회지 Vol.35 No.7
본 연구의 목적은 가축사체 매몰지 토양의 침출수 오염에 따른 병원성 미생물에 의한 잠재적 위해성을 평가하기 위하여 미생물 군집을 조사하는 것이다. 경기도 지역에 위치한 가축사체 매몰지 세 군데(A, B, C) 토양을 대상으로 DNA를 추출하여, 16S rRNA 염기서열을 분석을 통해 미생물 군집을 조사하였다. 연구결과를 문(phylum)별로 구분해보면, A 토양은 전체 토양미생물이 Proteobacteria (100%) 1개의 문으로 동정되었으며, B 토양은 Actinobacteria (66.4%) > Proteobacteria (31.1%) >Bacteriodetes (2.1%) > Acidobacteria (0.3%) 순으로, C 토양은 Actinobacteria (63.1%) > Proteobacteria (36.9%) 순으로 분포하였다. 속(genus)별로 구분해보면, A 토양에서는 Pseudomonas가 98% 비율로 나타났고,B와 C 토양의 경우 Arthrobacter이 각각 68, 61%로 우점하였다. 세 군데(A, B, C) 토양 미생물 군집의 종 다양성을 Shannon 지수에 근거하여 분석한 결과, B 토양(3.45)과C 토양(3.43)은 유사한 수준이었으나, A 토양(2.37)은 가장 낮게 계산되었다. 또한, 분석결과 Salmonella, Campylobacter 그리고 Clostridium perfringens과 같은 병원균도 발견되지 않았으나, 세균혈증을 일으키는 Ralstonia pickettii가 높은 농도로 관찰되었다. 본 연구에 사용된 가축 매몰지 토양은 침출수에 의한 미생물학적 오염도가 낮은 것으로 판단되지만, 가축매몰에 따른 병원성 미생물에 의한 토양의 잠재적 위해성을 평가하기 위하여 지속적인 모니터링이 필요하다. The aim of this study was to investigate the microbial communities in animal carcass disposal soils to examine the possible threat of pathogens from leachate. DNA extraction was performed for the soils in three carcass disposal sites located in Gyeonggi-do, Korea, and then 16S rRNA pyrosequencing was conducted to identify the microbial communities. Results indicate that, according to phylum classification, Proteobacteria (100%) was identified in soil A, Actinobacteria (66.4%) > Proteobacteria (31.1%) > Bacteriodetes (2.1%) > Acidobacteria (0.3%) in soil B, and Actinobacteria (63.1%) > Proteobacteria (36.9%) in soil C. According to genus classification, Pseudomonas was dominant in soil A (98%), Arthrobacter in soil B (68%) and C (61%). There were no detections of pathogens such as Salmonella, Campylobacter and Clostridium perfringens. However, high concentration of Ralstonia pickettii causing bacteremia was observed. Although carcass disposal soils examined in this study were not highly contaminated with pathogens, further monitoring is still needed to examine the potential threat of pathogens in leachate derived from carcass disposal sites.
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박정안 ( Jeong-ann Park ),이창구 ( Chang-gu Lee ),김재현 ( Jae-hyun Kim ),윤서영 ( Seo-young Yoon ),이인걸 ( In-geol Yi ),김성배 ( Song-bae Kim ) 한국농공학회 2013 한국농공학회 학술대회초록집 Vol.2013 No.-
본 연구에서는 다양한 철 산화물(마그헤마이트, 헤마타이트)과 박테리오파지 MS2간의 상호작용을 이론적으로 분석하기 위해 sphere=sphere DLVO (Derjaguin-Landau-Verwey-Overbeek)이론과 소수성 파라미터가 결합된 수정된 DLVO이론인 XDLVO (Extended DLVO)이론을 비교하고자 한다. DLCO이론에 따르면, 철산화물과 MS2의 거리간의 에너지는 반데르발스힘 (Van der waals), 전기 이중층 반발력(electrostatic double layer), 그리고 Born repulsion 에너지의 합으로 나타낼 수 있다. 여기에 XDLVO이론은 Lewis 산-염기 전자공여체-전자수용체 작용 에너지의 합을 추가함으로서 두 다른 구형분자의 소수성 에너지를 고려할 수 있게 해준다. 분석결과 DLVO이론과 XDLVO이론 모두 흡ㆍ탈착의 에너지장벽인 Φ<sub>max</sub> (primary maximum)값이 마그헤마이트가 헤마타이트의 값보다 낮게 추정되었으며, 간격이 증가되면서 나타나는 에너지인 Φ<sub>min2</sub> (secondary minimum)값도 마그헤마이트가 헤마타이트의 값보다 낮게 계산되었다. 이는 Lewis 산-염기 반응이 에너지 장벽을 낮춤으로서 바이러스와 철산화물의 흡착을 증진시킨다는 것을 의미한다. 값을 살펴보면 바이러스와 철산화물간에 흡착이 잘 일어날 수 있는 것(favorable adsorption)을 알 수 있으며, 이는 실제 흡착능을 평가한 배치 실험 결과와도 일치되는 결과로서 MS2 초기농도 5.00×10<sup>7</sup> pfu/ml에서 마그헤마이트의 흡착능은 (1.41±0.12)×10<sup>10</sup> pfu/g, 헤마타이트의 흡착능은 (9.10±1.20)×10<sup>9</sup>pfu/g으로 각각 나타났다.
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박정안 ( Jeong Ann Park ),김성배 ( Song Bae Kim ),김민선 ( Min Sun Kim ),권구흥 ( Ku Hung Kwon ),최낙철 ( Nag Choul Choi ) 한국물환경학회 2010 한국물환경학회지 Vol.26 No.6
The objective of this study was to develop automatic event detection algorithm for groundwater level rise. The groundwater level data and rainfall data in July and August at 37 locations nationwide were analyzed to develop the algorithm for groundwater level rise due to rainfall. In addition, the algorithm for groundwater level rise by ice melting and ground freezing was developed through the analysis of groundwater level data in January. The algorithm for groundwater level rise by rainfall was composed of three parts, including correlation between previous rainfall and groundwater level, simple linear regression analysis between previous rainfall and groundwater level, and diagnosis of groundwater level rise due to new rainfall. About 49% of the analyzed data was successfully simulated for groundwater level rise by rainfall. The algorithm for groundwater level rise due to ice melting and ground freezing included graphic analysis for groundwater level versus time (day), simple linear regression analysis for groundwater level versus time, and diagnosis of groundwater level rise by new ice melting and ground freezing. Around 37% of the analyzed data was successfully simulated for groundwater level rise due to ice melting and ground freezing. The algorithms from this study would help develop strategies for sustainable development and conservation of groundwater resources.
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