김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

여행업문화가 조직유효성에 미치는 영향에 관한 연구

이선희,이용근 문화관광연구학회 1999 문화관광연구 Vol.1 No.1

ABSTRACTAn Influence of Travel Agency's Culture on Organizational Effectiveness Sun-Hee Lee·Yong-Keun Lee The Effects of corporate culture on the organizational effectiveness and/or corporate competitiveness have been widely recognized and discussed among both the incumbent managerial and the academicians of economics and sociology in recent years. The purpose of this study is to review the functions of the corporate culture and to examine the factors of the corporate culture among the travel agencies.The present study starts with a conceptual review of the corporate culture. The characteristics of corporate culture is shaped usually with the two major elements; the styles of management and decision-making of the top managerial and the subculture of the employees. Each of the four components of Corporate culture is operationalized as follows; ① tough-guy, macho culture, ② work hard, play hard culture, ③ bet-your-company culture, ④ process culture. These four components of corporate culture become the independent variables while the recognition of environment becomes the dependent variables in the analytical model proposed in this paper. The recognition of environment such as danger and feed-back speed is divided into pre-IMF and post-IMF.Some results of the findings are as follows: First, before IMF, as the danger is recognized low and feed-back speed fast, the corporate culture is shown as work hard, play hard culture. Second, after IMF, as the danger is recognized high and feed-back speed fast, the corporate culture is shown as tough-guy, macho culture.

추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 1종의 Sterol과 3종의 배당체 화합물의 단리·동정

이유건(Yu Geon Lee1),조정용(Jeong-Yong Cho),이현주(Hyun Joo Lee),이용현(Yong Hyun Lee),이상현(Sang-Hyun Lee),한태호(Tae-Ho Han),김월수(Wol-Soo Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5

본 연구에서는 배의 유용성 증명을 위한 일환으로 배의 화학성분을 분자수준에서 밝히고자 하였다. 이에 배 과피 MeOH 추출물을 용매분획하여 얻은 EtOAc-산성 분획과 EtOAc-중성분획을 대상으로 Sephadex LH-20, silica gel, 그리고 ODS colmn chromatography와 HPLC를 이용하여 정제 및 단리하였다. 그 결과, EtOAc-산성 분획과 EtOAc-중성 분획으로부터 각각 2종씩의 화합물을 단리하였다. 단리된 화합물 1-4는 1H- 및 13C-NMR 분석을 통하여 각각 (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (화합물 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone(piceoside, 화합물 2), β-sitosterol (화합물 3), 그리고 β-sitosteryl 3-O-β-D-glucopyranoside (화합물 4)로 동정되었다. 단리된 3종의 배당체 화합물(화합물 1, 2, 4)들은 본 연구에 의해 배로부터 처음 동정되었으며, 화합물 3은 추황배로부터 처음 동정되었다. 본 연구결과가 배 함유 성분연구는 물론 배의 기능성 해명 연구에도 추후 중요한 기초자료로 활용되길 기대한다. We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.

지난 2년간의 학회지 "신경정신의학" 심사 평가보고

김찬형,김세주,김도관,김성곤,박원명,송동호,박용천,이영문,오강섭,정영철,이상열,김주한,이동영,이헌정,함병주,김율리,김문두,김정진,이강욱,천근아,김재진,홍진표,홍승철 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.6

디지털 영상을 이용한 수복재의 변색에 관한 연구

이용근,이건일,임미경 대한치과기재학회 1996 대한치과재료학회지 Vol.23 No.2

The purpose of this study was to find correlations among the color differences measured by three methods. Those were 1) spectrophotometric method(Differential Colorimeter, Model TC-6FX, Denshoku, Japan), 2) digital image method-photographed by digital camera(KODAK Digital Camera 40, Kodak, U.S.A.) and processed by computer program(Adobe Photoshop 3.0), 3) 35 mm standard slide method-took photographic color slide, read using scanner(Nikon 35 mm Film scanner, LS-3510 AF, Nikon, Japan) and processed by computer program(Adobe Photoshop 3.0). The color of resin modified glass ionomers were determined in vitro before and after thermocycling for 500 to 2,000 cycles. The values of CIELAB ΔE*, L*, a*, b*, r, g, b, r*, g*,b* were analyzed to find correlations among them. From the experiment, the following results were obtained: 1. There were no statistically significant correlation among the values of CIELAB ΔE*, ΔC measured by digital camera and ΔC* measured by color slide and scanner (Sig. F>0.05). 2. There was a statistically significant correlation between the values of CIELAB L* and GR measured by digital camera(Sig. F=0), and some pairs showed significant correlation among CIELAB a*, b* value and r, g, b value determined by digital camera and r*, g*, b* value determined by color slide and scanner. 3. The values of r, g, b determined by digital camera, and the values of r*, g*, b* value determined by color slide and scanner showed significant correlation between each corresponding values (Sig. F=0). In this case, correlation coeffcient were 0.339-0.413. 4. In the same materials, the degree of color was different depending on the shades, and of some shades showed of materials clearly different color change depending the measuring method.

운동 중 지방 대사에 대한 고찰

이근일,백병주,김의영,김도준,조용철,정훈,조재혁 龍仁大學校 體育科學硏究所 2002 體育科學硏究論叢 Vol.12 No.1

Glucose-fatty acid cycle is very important because it explains that fatty acid influences glucose level. Glucose-fatty acid cycle is the key factor to understand how endurance training which aids fat oxidation and carbohydrate stored in skeletal muscle influence mitochondria level. However, it is not certain whether glucose-fatty acid cycle influences exercising skeletal muscle of human being. As an energy resource, training increases use of fatty acid, endurance ability, and oxidation of triglyceride. During high level training, catecholamine secretions and restraint of fatty acid use caused by insulin increases dramatically, this results in reduction of free fat acids and reunification of free fat acids into triglyceride. Training restrains insulin activity and reduces plasma acid density, however, elite athletes have high triglyceride-fatty acid cycle. Although enzyme's activities and mitochondira's density increase after training, it's not certain that enzyme's activities is major factor in restraint of fatty acid oxidation in exercising muscle cells. Stores of fat leads to increases of fat oxidation, performance and reduces use of carbohydrate, however, there is no absolute evidence to support these results.

淋菌의 藥劑耐性樣相에 對한 調査硏究

李承薰,石鍾聲,朴蕙瓊,金景俊,柳在根,李龍雨 國立保健硏究院 1973 국립보건연구원보 Vol.10 No.-

광중합형 글라스아이오노머의 열순환에 따른 경도변화

이용근,임미경,구대회 대한치과기재학회 1996 대한치과재료학회지 Vol.23 No.2

The purpose of this study was to compare the effects of thermocycling on the change of Vickers hardness of four resin-modified glass ionomers depending on material types and shades. A conventional glass ionomer and a resin composite material were used as controls. Disk specimens were prepared and polished and Vickers hardness was measured. After thermocycling in 5~55℃ distilled water for 1,000 and 2,000 cycles, the Vickers hardness was measured. The results were analyzed by the type of materials, and the shade of the materials in the same material. The following results were obtained. 1. The Vickers hardness numbers of Z10 group were statistically higher than those of resin modified glass ionomers regardless of the thermocycling(p<0.05), and those of conventional type Ⅱ glass ionomers were significantly lower than all the other groups(p<0.05). 2. The increase ratio of the Vickers hardness of VGL group was significantly higher than those of other groups up to thermocycling for 1,000 cycles(p<0.05), and the Vickers hardness of Z10 group decreased more than 10%. The increase ratio of Vickers hardness of Z10 and FJT group was significantly higher than those of the other groups during thermocyling from 1,000 to 2,000 cycles(p<0.05). 3. Comparing the Vickers hardness of before-thermocycling and after-2,000 thermocycling, the Vickers hardness numbers of FJT, VGL and FLC group increased by 11~21 % after thermocycling, and those of DRT, Z10 and VTM group decreased by 5~1% after thermocyclig. 4. The tendency of microhardness change was different depending on the shade of materials, but there was no clear trend.

치면열구전색제의 조건에 따른 법랑질 결합강도에 관한 연구

이용근,이흥수,이성재,방기숙,최정민,류승호,강민석 대한치과기재학회 1997 대한치과재료학회지 Vol.24 No.1

In this study, the shear bond strengths and accompanying elastic modulus ratio, displacement at bond failure and work up to fracture of pit and fissure sealants to toothe enamel were analyzed by the following variables, those were acid etching, moisture content of the surface of enamel, dentin bonding agent(species and method), thermocycling, saliva contamination. One kind of chemically cured resin based pit and fissure sealant, two kinds of light cured resin based pit and fissure sealants and one kind of glass ionomer based pit and fissure sealant, and two kinds of dentin bonding agents were studied. The buccal surface of extracted premolars were ground flat with # 600 silicone carbide papers, and the ground surface was treated depending on the experimental conditions. After each treatment, each pit and fissure sealant was filled into plastic tube of 3mm diameter, which were laid on the surface of ground enamel surface, and were cured. Sealant bonded teeth specimens were immersed in 37℃ distilled water form 24 hours. The shear bond strength of sealant to enamel surface was measured with Universal testing machine with the cross head speed of 1mm/min. From the obtained load-deflection curves, modulus ratio, bond strength, displacement at bond failure and work up to fracture were analyzed. From this experiment, the following results were obtained : 1. Comparing the results of standard condition(i. e. acid etching, dry surface, no dentin bonding agent and 300 thermocyling), the elastic modulus ratio of chemically cured resinbased pit and fissure sealant was significantly higher than those of other materials(P<0.05), and the bond strength, displacement at bond failure and work up to fracture of glass ionomer based pit and fissure sealant were significantly lower than those of other materials(P<0.05). 2. The results of ANOVA with the variables of type of material, acid etching, moisture condition of the enamel surface, dentin bonding agents were as follows ; 1) variables showed significant main effect on the shear bond strength of pit and fissure sealant to enamel(P<0.01), 2) type of material, acid etching and moisture condition of enamel influenced the bond strength significantly(P<0.01), 3) type and application methods of dentin bonding agents influenced on the bond strength significantly(P<0.05). 3. There were statistically significant correlation between the work up to fracture and the bond strength, and between the work up to fracture and the displacement at bond failure(P<0.01), and in these cases the correlation coefficient was 0.87.

산도를 조절한 불화석 용액의 치과 응용에 관한 연구 : 제 1 편 : 불화석 용액과 완충액에 의한 치과 수복재의 표면변화

이용근,이성재,이흥수 대한치과기재학회 1997 대한치과재료학회지 Vol.24 No.1

Topical fluoride application reduces the incidence of dental caries, and it is well known that hydrofluoric acid etches glass and ceramic. Repeated exposure of dental ceramic or composite resin to acidulated phosphate fluoride can result in material loss, surface roughening, and corresponding change of esthetic appearance. The effect of APF preparations on dental ceramic or composite restorations was reported, but the influence of fluoride solutions routinely used for mouth rinsing or topical application is unclear. And the acidity of fluoride solutions can influence the surface change of glass containing dental materials. In this study, the effect of stannous fluoride solutions and buffer solutions of different acidity on esthetic filling materials was determined. Five kinds of composite resins(AEF, HPL, POF, TPH, Z100) and two kind of glass ionomers for filling(FJT, FLC) were studied. Specimens of 8mm in diameter and 2mm in thickness were made and fine polished, after then vickers hardness and color of the specimens were measured. Five kinds of immersion solutions were used, they were distilled water(CTRL) as control, dibasic sodium phosphate buffer(SFPB) and in distilled water(SFDW). Specimens were immersed in each solution and thermocycled for 500 cycles between 5℃ and 55℃. After then, Vickers hardness and color of the specimens were measured and compared with results before immersion. From this experiment, the following results were obtained. 1. The color differences(CIELAB ΔE*) of AEF, TPH, Z100, FJT and FLC in SFPB, in most cases, significantly higher than those in other solutions(P<0.05), and those of HPL and POF in SFDW was significantly higher than those in all the other solutions(P<0.05). 2. Comparing the result in the same immersion solution, color differences(CIELAB ΔE*) of different materials in CTRL were not significantly different from each other(P>0.05), but in other cases, the results were different depending on the material and solution combinations. 3. Change of Vickers hardness after treatment of resin-modified glass ionomer in PB2.5 was significantly higher than those in all the other solutions(P<0.05), and one kind of composite significantly influenced by the acidity of fluoride solution.