정상 한국인에서 연령증가에 따른 혈장 Dehydroepiandrosteronesulfate ( DHEA-S ) 농도 및 DHEA - S / cortisol 비의 변화

박성우,유형준,임성희,최문기,유재명,박민숙,이권엽,박철수,김철홍,김현규,김두만,박규용 대한내분비학회 1997 Endocrinology and metabolism Vol.12 No.2

Backgroud: DHEA-S is the most abundant steroid hormone in circulation, and primarily secreted from the adrenal cortex, but its physiological role is little known. One of the characteristic features of DHEA-S is progressive decrement of plasma DHEA-S level with advancing age, in contrast, plasma levels of other adrenal hormones are not chaging or littie decreasing. To grasp the trends of plasma DHEA-S level and DHEA-S/cortisol ratio by age in healthy Korean, we measured the plasma DHEA-S levels and DHEA-S/cortisol ratios in healthy Korean. Methods: Healthy Korean(men: 99, women: 102, age range: 15-97 year old)were studied. Subjects were not taking drugs(such as glucocorticoid or androgenic medication) or cigarettes known to modify the plasma level of DHEA-S and cortisol, and had no evidence of hepatic, renal disease or hyperlipidemia as determined by serum lipid, bilirubin, SGOT, SGPT, BUN, creatinine. Data were analyzed by 10-year age group for men and women: i.e, 10-19, 20-29, 30-39, 40- 49, 50-59, 60-69, 70-79, 80-89 and 90 year or more. Plasma DHEA-S levels were measured by using a commercially available RIA kit with 125I labeled-DHEA-SO4(Coat-A Count DHEA-SO4), and for the measurement of plasma cortisol levels, commercial Gamma Coat TM[125I] Cortisol Radioimmunassay Kit was used. Results:. 1) In both men and women, plasma DHEA-S level showed high interindividual variation within the same age group. 2) There were individual sex differences in plasma levels of DHEA-S, in all age groups, plasma DHEA-S levels were significantly higher values for men than for women. 3) Maximum plasma DHEA-S levels(men; 237+-3.35 ug/dL, women; 108+-17.5 ug/dL) were at third decade in both men and women. 4) Both men and women showed the continuous decline in plasma DHEA-S level with age. These age-related decline was more prominent in men than in women(men; y= -3.152 * +292.6, r2= 0.8459, P$lt;0.05, women; y= -1.417 * +143.3, r2 = 0.7278, P$lt; 0.05). 5) As an index of aging, there was no stastical difference between DHEA-S and DHEA- S/cortisol ratio. Conclusion: In healthy Korean, there were high interindividual variation of plasrna DHEA-S levels. In both men and women plasma DHEA-S level was peak at third decade, and from when it declined progressively with age. These results suggest that although the reliability of single plasma DHEA-S measurement are limited, the decline of DHEA-S with advancing age might be a specific marker of endocrinologic hormonal milieu(aging index). Also, concerning to individual adrenal secreting capacity, we measured DHEA-S/cortisol ratio. But we did not found that plasma DHEA-S/cortisol ratio is superior to the plasma DHEA-S level as an aging index. (J Kor Soc Endocrinol 12:245-254, 1997)

T 세포활성항원 CTLA-4의 기능에 관한 연구 : T 세포에서 표적세포 살해능과 CTLA-4 발현과의 연관성 Target Cytotoxicity of T Cell Correlates with CTLA-4 Production

노만택,조양자,김용식,최용,조보현,최장원,정용훈 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.4

CTLA-4, a T cell activation antigen and a homologue of CD28, was originally identified as a gene by a series of reverse genetic approaches. While CD28 molecule has been characterized well as a stimulator of T-cell function via enhanced lymphokines production and stablilization of those mRNA, most of the functions of CTLA-4 remain unknown. It has been widely accepted that CTLA-4 functions as an immune suppressor which is down-regualting the function of CD28. We previously showed that 34 KD form of this antigen mainly expressed CD8+ subset, a cytotoxic or suppressor, of activated peripheral blood lymphocyte. Based on our previous finding this study was conducted to further characterize immunological function of CTLA-4 especially in terms of involvement of this molecule in T-cell effector function mediating target cell cytotoxicity. 4 human T cell clones with different target cytotoxicities were employed in this study. NBL46 (CD4+) and NBL77 (CD8+) were cytotoxic and NBL32 (CD4+) and NBL58 (CD 8+) were non-cytotoxic to target LBF cell in target cell chromium release assay. And in Western blot assay 34 kD antigen was detected in NBL46 (CD4+) and NBL77 (CD8+) clones but not in NBL32 (CD4+) and NBL58 (CD8+) clones. It was summarized that expression of the CTLA-4 antigen was associated with cytotoxicity but not with subset phenotypes of T cells. In conclusion CD8+ T subset of PHA-stimulated PBL was major and only CTLA-4 producer and this molecule was induced during mid to late stage of T cell activation. The cytotoxicity of T cell clones to its target cell was directly correlated with its CTLA-4 production and vice versa. And it was highly suggested that primary function of CTLA-4 may involved in T cell effector function which mediates target cell killing.

당뇨병 환자의 사례관리 실시 전후 혈당 및 자가관리 이행수준 변화

최용우,이용환,전만중,유병철 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Background : Diabetes mellitus is one of the common chronic disease in Korea. As most chronic diseases need continuous cares and behavioral controls, one of the effective managements for diabetes is maintenance healthy life style which is based on correct medical knowledge. So, we conducted this study to assess change of blood sugar levels and self-management behavior of diabetic patients following the case-management program enforced by National Health Insurance Corporation (NHIC). Methods : We conducted this study during the period from January 2006 to December 2006, 29 case managers performed the case management program for 672 diabetic patients in the districts of Busan, Woolsan, and Gyeongnam. We evaluated the effectiveness of the case management program based on the changes of blood sugar level, knowledge for diabetes and self-management behavior of diabetic patients. Data analysis was performed with t-test, paired t-test, McNemar X2-test and correlation analysis using SPSS program(ver 12.0). Results : After the case management program, smoking rate was reduced from 22.3% to 21.4% and exercise rate was increased from 65.0% to 80.2%(p<0.05). The knowledge standard of diabetics on 10-point scale revealed considerable increase from 6.05 before the management to 8.69 after the case management(p<0.05). All of diabetic self-management behaviors including nutrition management, self-test on blood sugar level, foot care, oral care and diabetic medication showed considerable improvement; the overall behavioral change, on a 5-point scale, improved from 3.25 to 3.75(p<0.05). The FBS level was improved from 138.4mg/dl to 131.5mg/dl and the blood sugar level 2 hours after a meal was improved from 203.7mg/dl to 183.5mg/dl(p<0.05). Conclusion : From the above results, we concluded that the case management program enforced by the NHIC showed positive behavioral changes on smoking and exercise, increased knowledge about diabetes as well as the level of nutrition management and also demonstrated decline of blood sugar levels. So, we need research of customized information for every patients rather than generally summarized information on diabetes and a self-dependent group formations for sustaining improved self-management after case management as well as development of education and training programs for case managers.

적외선 열화상을 이용한 비파괴시험 활용 및 결함 진단

최만용,김원태 한국비파괴검사학회 2004 한국비파괴검사학회지 Vol.24 No.5

본 고는 적외선 열화상의 개념과 적외선일화상 측정원리 및 카메라 셋팅을 기술하고 적외선일화상(IRT)에 의한 비파괴검사(NDT)의 활용 및 비파괴검사의 진단에 대하여 살펴보았다. 적외선 일화상은 주어진 표면을 따라 온도에 관련된 일패턴의 평가를 통한 정기적인 비접촉, 비파괴 시험의 수행으로 초기에 장비 고장의 예방이 가능하다. 진단 활용으로서, 열적으로 가열된 내부결함이 있는 블록에 대하여 적외선열화상을 이용한 열화상 패턴을 비파괴 기법으로 평가하고, 결함과 열화상패턴간의 특성을 분석하여 열화상 평가 기법에 대하여 논하였다. In this paper, the concept of infrared thermography(IRT), the principle of measurement of IRT and how to set up the 111 camera for the nondestructive testing are described in detail. Also, its utilization and non-destructive testing(NDT) diagnosis are reviewed. By performing the periodic non-touched NDT through the estimation of thermal patterns related with the temperature for the surface targeted, IRT can be applied to the early prevention of the device failure. For the diagnosis utilization, thermal imaging patterns obtained from IRT for heated blocks with internal defects were estimated through the non-destructive method and discussed the way of IRT estimation from the analysis of characteristics between material defects and thermal imaging patterns.

6주간의 淸暑益氣湯 經口投與가 에너지대사 및 호르몬 변화에 미치는 영향

최용어,강명신,유종만,이규성,오재근,조준용 韓國體育大學校附屬 體育科學硏究所 1994 韓國體育大學校附屬 體育科學硏究所論文集 Vol.13 No.1

The purpose of this study was to investigate the effects of chung-seo-ik-gi -tang administration on the metabolic responses and hormones in 18 healthy-male elite boxes. All subjects were divided randomly with two groups, Chung-sea-ik-gi-tang Group(N=18) and placebo group(N=18) and perfored with 70% of HR max using bycicle ergometer unfil exhaustion. During the submaximal exercise, heart rate was maesured every two min. The blood samples were collected from antecubital vein by 10ml syranges(6ml/each) before immediately after exhaustion, recover-10 min, recover-20 min and recover-30 min. The samples were to analyzed for the level of glucose, lactate, TG, TC, HDL-C, insulin and catecholamine. The results of various blood parameters were summarized as follow; l. Energy substrates (plasma glucose, TG, TC, HDL-C) changes in group C were significantly different from group P. Plasma glucose in group C was significantly lowered at recover-10 min compared to group P (P<0.05). and also TG, TC and HDL-C in group C were significantly increased after 3 weeks compared to group P (P<0.05) whereas. Lactate accumulation in group C was significantly decreased after 3 weeks compared to group P (P<0.05). 2. Plasma insulin level was not showed significant difference between two groups. But insulin depression tendency after 3, 6 weeks in group C was allevated compared to group P. Increasing rate of norepinephrine in group C at Post-exhaustion after 3weeks lowered compared to group P. Norepinephrine in group C at rest periods after 3 weeks showed significant difference compared to group C. The results indicated that Chung-seo-ik-gi-tang had the effects of ergogenic aids on fatigue resistance and recovery from exhaustive exercise at the level of 70% HR max.

불국사 석탑의 물성 및 지반특성 조사

최희수,서만철,오진용 공주대학교 문화재보존과학연구소 2003 문화재과학기술 Vol.2 No.1

경주 불국사의 다보탑과 석가탑은 751년에 축조된 국보급 문화재이나 오늘날은 석재의 풍화 및 지대석의 어긋남 현상들이 관찰된다. 이에 대한 정확한 이해는 두 석탑의 안정성 진단에 필수적이며 따라서 석탑의 지반환경과 석재 물성에 관한 비파괴 정밀 조사가 필요로 한다. 이 연구에서는 석재의 역학적 성질을 위해 초음파 속도 측정을 실시하였고 천부지질구조를 위해 탄성파, 전기비저항, 지하레이더 등의 비파괴 복합 지구물리탐사를 석탑 주변에서 수행하였다. 초음파 속도범위는 석가탑에서는 1217~4403 m/s이며, 다보탑에서는 584~5845 m/s이며 이로부터 추정한 일축강도 평균치는 각각 463 kg/cm^(2), 409 kg/cm^(2)이다. 대체로 두 석탑 주변의 북서쪽 구역에서 낮은 탄성파 속도와 비저항 값이 나타나, 이 부분을 비교적 연약한 지반으로 해석한다. 그리고, 탄성파 속도분포에서는 다보탑 지반이 더 큰 속도를 보여 상대적으로 석가탑 지반보다 견고함을 보여준다. 대체로 석탑지반의 전기비저항은 최대 2200 Ωm이며 200 Ωm내외의 외부지반보다 더 높은 값을 보인다. 복합 지구물리탐사 결과를 종합하면 다보탑지반의 형태는 8각형(한 변은 6 m)으로 그 깊이는 약 4 m 이나, 석가탑지반은 약 8x10 m의 직사각형 형태로 깊이는 약 3 m 이다. Two three-story stone pagodas, Seokgatap and Dabotap, built in 791 at Bulkuksa temple, are well-known national treasures. However, they have experienced weathering process and an offset of the stone alignment is observed at the base part of Dabotap. Understanding of these phenomena is essential for the structural safety diagnosis for them, and therefore the nondestructive geophysical methods was applied. Direct measurements of ultrasonic velocities was introduced for the mechanical properties of the stone. Multidisciplinary geophysical prospectings including seismic, resistivity, ground-penetrating radar(GPR) methods were carried out around two pagodas for their site characteristics. The velocity ranges of ultrasonic waves for Dabotap and Seokgatap are 1217~4403m/s and 584~5845m/s, respectively, and the estimated averages of the uniaxial compressive strength are 463 kg/cm^(2) and 409 kg/cm^(2), respectively. At the northeast of each pagodas, the lower seismic velocities and resistivities are shown, which is interpreted to be weaker subsurface section. On the basis of the higher velocity structure, the site of Seokgatap appears to have solider stability than the Seokgatap site. Near the pagodas, higher(up to 2200 Ωm) resistivity is present whereas their outskirts have as low as 200 Ωm. By the combined results of each geophyscial methods, the subsurface boundaries of each stone pagodas are revealed. The Dabotap site is in the form of an octagon having 6-m-long side with the depth of ~4 m, whereas the Seokgatap site is the 8x10 m rectangle with the depth of 3 m.

CGI 프로그램에서 클라이언트와 서버의 자동화된 상태정보 전달

최준용,유진호,장종표,방만원 木浦大學校 情報産業硏究所 1997 情報産業硏究誌 Vol.5 No.-

The HTTP Protocol composed of WWW's client/server model is unconnected and not keep state, so, there is one connection for one request. In this condition, it has no guarantee to memory which is needed in sequential processing, and HTTP Protocol has no choices to transfer the information from client to server's CGI or the information exchange. This paper uses the word 'key' instead of this 'state information'. We studied the key which creates automatically the HTML document for data search hierarchically in WWW environment and to confirm applied this to multimedia order and sale system of agricultural and marine products.

폴록사머를 이용한 디클로페낙 고형 좌제의 개발

용철순,오유경,김정애,김용일,박상만,양준호,이종달,최한곤 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.2

To develop a poloxamer-based solid suppository with poloxamer mixtures, the melting points of various formulations composed of P 124 and P 188 were investigated. To investigate the effect of poloxamer to the dissolution and dissolution mechanism of diclofenac sodium from the suppository the dissolution of diclofenac sodium delivered by the poloxamer-based suppository was performed. Furthermore, to investigate the mucoadhesive property of the poloxamer-based solid suppository, the identification test in the rectum was carried out after its rectal administration in rats. The poloxamer mixtures composed of P 124 and P 188 were homogeneous. Very small amounts of P 188 affected the melting points of poloxamer mixtures. In particular, the poloxamer mixture [P 124/P 188 (97/3%)] with the melting point of about 32℃ was a solid form at room temperature and instantly melted at physiological temperature. Furthermore, very small amounts of P 188 in the poloxamer-based suppository hardly affected the dissolution rates of diclofenac sodium from the suppository. Dissolution mechanism analysis showed the dissolution of diclofenac sodium was proportional to the time. At 4 h after administration, the blue color of poloxamer-based suppository [diclofenac sodium/poloxamer mixture (2.5/97.5%)] with the P 124/P 188 ratio of (97/3%) and blue lake in the rectum was faded. However, the position of suppository in the rectum did not significantly change with time. Thus, it retained in the rectum for at least 4 h. Our results indicated that the poloxamer-based solid suppository with P 124 and P 188 would be a candidate of rectal dosage from for diclofenac sodium.

Aluminium板의 環境破壞에 關한 基礎的 硏究

崔壯宇,金榮萬,李龍福 弘益大學校 1982 弘大論叢 Vol.14 No.2

It is known that the most of Enviroment Assisted Fracture affected remarkably by the various environmental effect are brittle fracture, and that the above field is very wide. In this paper, Environment Assisted Fracture is discussed from the view point of fracture mechanics. Aluminium and SUS304 Stainless Steel in solutions of several percentage of MgCl₂(10%, 15%, 20%) at the temperature(60℃±3℃)is investigated according to the crack growth, and the value of K is compared with the K obtained by the changes of deflection according to the crack growth. THe results of the experiment is summarized as following 1)The tendency of crack growth as time goes by is shown as 4 region in both materials. 2)The experimental value of K of SUS304 Stainless Steel is same as the calculated value from the variation of the compliance according to crack growth, but not in Aluminium.

팥의 RAPD 분석 조건 최적화 연구

이충열,박현철,박진철,김성만,김용철,최재희,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

The object of this study was to optimize PCR condition for RAPD analysis in adzuki bean. The best template DNA concentration was 20ng(0.25unit taq polymerase and 2.5mM MgCl2), 40ng(1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2 and lunit taq polymerase and 7.0mM MgCl2), and 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, and 1unit taq polymerase and 4.5mM MgCl2) The best MgCl2 concentration was 2.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), 4.5mM(20ng template DNA and 0.25unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), and 7.0mM(40ng template DNA and 1unit taq polymerase). Amount of taq polymerase was 0.25unit(20ng template DNA and 2.5mM MgCl2), 0.5unit(60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2) and 1unit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, and 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, the best condition for PCR optimization was 60ng template DNA, 4.5mM MgCl2, and 1unit taq polymerase. Reaction temperatures for the optimal PCR condition were 84℃, 32℃, 62℃; 90℃, 40℃, 72℃; and 92℃, 36℃, 72℃.