Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

1~2기 본태성 고혈압 환자들에서 Imidapril 대 Enalapril의 항고혈압 효과를 평가하기 위한 무작위, 이중맹검 임상시험

최영진,손대원,김용진,이홍자,채인호,김효수,김철호,오병희,이명묵,박영배,채윤식,이영우 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11

食用 고사리의 Thiaminase에 관한 硏究

崔瑢鎭,南昌祐 同德女子大學校 1977 同大論叢 Vol.7 No.1

In the course of studies on a thiaminase from wild bracken, Korean's common foodstuff, and its effects on the human nutrition, some properties of the enzyme were investigated. The obtained results were as follows; 1. From 10 g of the powdered bracken, thiaminase was extracted about 2,924 units of specific activity 10.99. 2. The bracken thiaminase exhibited the sharp optimum pH at a range of 7.5 to 8.0 for its activity. And the maximum reaction rate was achieved at the temperature of 60℃. 3. The Km value for the enzyme was calculated to be 1.54 x 10-³mole with respect to thiamine hydrochloride and 3.57 x 10-³mole with respect to pyridine. 4. Of the metal ions tested, only manganese ion was recognized to have a slight effect in activating the enzyme while heavy metal ions especially cupric salt showed a remarkable inhibitory effect. 5. The enzyme activity was strongly inhibited by p-chloromercuric benzoate, a typical "SH-reagent". But the whole activity was recovered by thereafter incubating with cysteine solution. Therefore the bracken thiaminase was considered to be an enzyme which possesses essential SH group in order to retain its normal active conformation. 6. On storage at low temperature below 5℃, the enzyme was greatly inactivated but it showed considerable stability at the temperature around 20℃. 7. The enzyme was best protected from thermal denaturation at pH 7.0 with phosphate buffer, but it lost almost all the activity by 10 minute's heating at 80℃.

현성 당뇨병이 없었던 알코올 간경화증 환자에서 발생한 공기증신우신장염 1례

최재혁,이용국,김강,김준섭,강혁주,탁우택,이정호,구관민 東國大學校醫學硏究所 2007 東國醫學 Vol.14 No.1

공기증신우신장염은 신실질의 괴사와 자발적인 공기 형성과 연관된 드문 감염질환이다. 대부분 혈당이 잘 조절되지 않는 당뇨 환자들에게서 발생하며 드물게 요로 폐색, 요로 결석, 종양 등에서도 발생한다. 과거 초기의 수술적 치료가 우선시 되었으나 최근에는 항생제 및 조기 진단으로 인해 내과적 치료만으로 성공한 사례가 증가하고 있다. 저자들은 현성 당뇨병이 없었던 알코올 간경화증 환자에서 발생한 공기증신우신장염을 내과적 치료 및 경피적 배농술만으로 치료한 예가 있어 보고하는 바이다. Emphysematous pyelonephritis is an uncommon infection of the kidney associated with parenchymal necrosis and spontaneous gas formation. Most cases occur in diabetic patients and rarely in urinary tract obstruction, stone or tumor. At past, surgical treatment was prior to internal treatment, but recently, successful cases of internal treatment have increased because of the development of antibiotics and early diagnosis. We report an emphysematous pyelonephrtis in 61 years-old non-overt diabetic woman with alcoholic liver cirrhosis, successfully treated by antibiotics and percutaneous drainage.

신호교차점 접근로의 지체해석에 관한 연구

우용한,김대웅,최용석 嶺南大學校 工業技術硏究所 1998 工業技術硏究所論文集 Vol.26 No.2

This study was a selection circular road of Taegu city and four intersections approach which is located in the suburbs as the subject of study. And then, observed the relation between volume, traffic, speed and distribution of vehicle speed. It was examined the goodness of fit after making a model through the decelerated delay, accelerated delay and stopped delay which are calculated on the basis of the survey data. And also looked into the relation among the approach delay and the stopped delay and vehicles delay of signalized intersection approach which was caused by the traffic congestion. In the comparison of the average stopped delay for the model, KHCM model have an observation values and an approximate value. It was known the fact that the prediction model (1) and (2) that is provided by this study can applied at present. It could use this result to the shortest route search method and the signal system operation, the calculation of the service level at the signalized intersections.

Pulse Power를 이용한 혐기성 소화 효율 증대

최한나,전용우,정윤진,홍승모 대한상하수도학회 2003 상하수도학회지 Vol.17 No.3

Anaerobic digestion is commonly used in wastewater treatment plants to stabilize sludge produced in primary and secondary treatment, to produce renewable energy as methane, and to reduce the volume of solids for ultimate disposal. Previous studies on anaerobic digestibility of primary and secondary sludge present that secondary sludge showed poor anaerobic digestibility and less gas production. The objectives of this research were to study the effects of pulse power pretreatment on anaerobic digestibility of waste activated sludge. These objectives were achieved through operation of carefully-controlled laboratory digesters under various conditions. The conclusion made from this study are as follows: 1) To maximize the efficiency of pulse power pretreatment for waste activated sludge, the optimum operation conditions for coaxial reactor with 7 rings are decided as follows; a) optimum pulse repetition rate : 110㎐, b)feeding service : once-through mode, c) optimum hydraulic retention time : 1.0-1.5sec 2) Pretreated WAS by pulse power system showed increase of SCOD, ECP, and V.A by 13.6, 4.6, and 7.1 times. 3) From the results from operation of lab scaled anaerobic digester, pulse power sludge pretreatment increased GPR from 0.39㎥gas/㎏VS_add · day to 0.66㎥gas/㎏VS_add · day and methane content from 43.9% to 87.9%. Thus, increase of methane production rate was increased by 3.4times. 4) During the steady state operation of anaerobic digester, the removal efficiency of TCOD and VS were improved by 44.6% and 31.5% respectively after pulse power pretreatment of waste activated sludge.

PCR을 이용한 국내 및 수입 축산물 유래 Listeria monocytogenes의 유전학적 분석연구

우용구,이수화,이철현,최정수,류재두,김영일,이오수,김봉환 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.2

One hundred-fifty two of Listeria strains were isolated from domestic chicken carcasses, a slaughter-houses from nationwide from 1996 to 1997 and imported livestock products from a ten foreign countries including the USA, China, France and Thailand. To substitute the laborious and time consuming procedures of clinical laboratory standard diagnostic methods, the rapid and specific multiplex PCR(M-PCR), which was designed to amplify a three kinds of genes simultaneously f3r specific detection and differentiation of L. monocytogenes(LM) by one step procedure, was established. The LM strains confirmed with M-PCR were analysed to their genetic diversities by RAPD using the two primers(D87 & MMTl), and also evaluated on their discriminatory abilities(na) between two primers. our study also conducted the REP-PCR and ERIC-PCR for evaluation of the possibility of REP and ERIC elements for genetic subtyping of LM strains. According to the REP-PCR and ERIC-PCR fingerprinting patterns, LM strains were divided into 7(ERIC-type) and 4(REP-type) major clusters at the relative clone cut off value of 80%. According to the computer analysis of PCR results using the GelCompar Ⅱ software, ERIC-PCR(DI=0.955) had expressed the most high discriminatory ability and followed by REP-PCR(DI=0.952), RAPD(D87; 0.954, MMT1; 0.937), in order. The combined analysis of the present PCR results was also expressed the reliable and useful discriminatory ability(DI=0.916). This study suggested that PCR based methods could be used as an reliable, reproducible, rapid, and highly discriminatory method for the genetic differentiation of genus Listeria.

冬蟲夏草의 抗轉移와 免疫增進에 關한 硏究

최우진,유화승,이용연,서상훈,조정효,이연월,손창규,조종관 대전대학교 한방병원 2000 惠和醫學 Vol.9 No.2

This experimental study was carried out to evaluate the effects of Cordiceps sinensis on the antimetastasis and immune activity. In order to investigate the effect of Cordiceps sinensis, the followings were performed; Cytotoxicity, inhibition of MMP-2 & MMP-9 gene expression, fraction of CD4+, CD8+, CD19+ in splenic cell, the moprphological change of splenic cells, gene expression of IL-12(p35), IL-12(p40), IFN-?? and splenic cell proliferation by CS-E. The results were obtained as follows. 1. CS-E did not present cytotoxic effect on L+14, B16-F10 melanoma cells and HMCB. 2. CS-E inhibited MMP-2 and MMP-9 gene expression more effectively compared with control group. 3. CS-E inhibited invasion of B16-F10 melanoma cells more effectively compared with control group. 4. CS-E helped CD4+, CD8+, CD19+ expression more effectively compared with control group. 5. CS-E activated phagocytic cells in spleen more effectively compared with control group. 6. CS-E helped IL-12(p35), IL-12(p40), IFN-?? gene expression in splenic cell more effectively compared with control group. 7. CS-E activated splenic cell proliferation more effectively compared with control group. From above findings, it is suggested that CS-E is able to inhibit metastasis of cancer and activate immune response system.

레이저 형광법에 의한 프로세싱 플라즈마중의 입자 계측에 관한 연구

최상태,이광식,하장호,전용우,박원주 嶺南大學校 工業技術硏究所 1998 工業技術硏究所論文集 Vol.26 No.2

Armorphous materials have various structures that is determined by different manufacturing conditions. That is, the photoelectric characteristics of films manufactured by different conditions are totally different. The growth mechanism of a-Si should be understood in order to controll the characteristics and structures of these kinds of materials. In this study, we try to elucidate the particle behaviours in a processing plasma using a low pressure glow discharge. And because of difficulties understanding discharge mechanism, we have done the processing so far depending on the experimental methods that compare the thin film produced by varing discharge parameter with the results of micromachinary. But, this has a limitation in the development because it regards the plasma as a blackbox. Therefore, because it needs to observe the discharge parameters and plasma background gas systematically, we try make sure that the particle behaviours be made clean by using laser fluorescence method which is predominant in a phenomina measurement and special resolution.

신비체험과 해리성향과의 관계

최낙경,이상혁,우용일,이만홍,현용호 대한신경정신의학회 2000 신경정신의학 Vol.39 No.4

신비체험은 종교의 핵심경험이라고 일컬어질 만큼 종교계에서 가치 있게 평가되고 있으며 여러 사회집단에서도 관심의 대상이 되어왔다. 오래 전부터 심리학자들과 정신의학자들이 신비체험에 대해 관심을 가져 왔지만 그것의 개인적이고 주관적인 특성 때문에 과학적인 접근이 어려웠으며 단지 신비체험의 특성을 나열하고 묘사하는데 그쳤다. 최근 일부 정신병리학자들은 신비체험을 해리현상의 하나로 보는 주장도 있다. 한편 해리는 어린 시절의 외상경험과 연관이 있고 정신병리가 많을수록 해리경향성이 높아진다는 보고가 있어 왔다. 본 연구는 종교인중 신비체험을 경험하지 않은 사람(비신비체험군) 35명을 대조군으로 하여 종교인중에서 신비체험을 경험한 사람(신비체험군) 42명과 해리경향성, 정신병리, 외상경험을 비교하였다. 연구도구로는 한국어판 해리경험 척도, 간이정신진단검사, 외상유무를 묻는 질문지를 이용하였다. 그 결과, 해리경향성은 신비체험군이 비시비체험군보다 유의미하게 높았으며 외상경험도 유의미하게 높게 보고하였다. 그러나 정신병리상의 두드러진 차이는 발견되지 않았다. Mystic experience is highly evaluated so as to be called as core of experience in the religious world and also becomes the subject of concern among various social groups. Psychologists and psychiatrists are concerned about it, but because of its individuality and subjectivity, the scien-tific approach was difficult so that only the description of mystic experience has been researched fo far. There is a current report saying that mystic experience is the one of the dissociative pheno-menon. And there are a lot of reports saying that dissociation is related to childhood trauma experience and rises as much as psychopathological tendencies exist. Consequently, based on the reference that mystic experience is connected with dissociation, the study has been progressed to find out the level of dissociation, psychopathology and trau-ma experience for religious people who experienced mystic experience but excluding patients. Among them, it has been compared between group of non-mystic experience(n=35) and group of mystic experience(n=42). We used Dissociative Experience scale-Korean version(DES-K), Symptom Checklist-90-Revised(SCL-90-R), questionnaire about trauma. The result is that the group of mystic experience had a significantly higher tendency to have dissociation and trauma experience than group of non-mystic experience. However, the differ-ence of psychopathology has not been found.