김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

여행업문화가 조직유효성에 미치는 영향에 관한 연구

이선희,이용근 문화관광연구학회 1999 문화관광연구 Vol.1 No.1

ABSTRACTAn Influence of Travel Agency's Culture on Organizational Effectiveness Sun-Hee Lee·Yong-Keun Lee The Effects of corporate culture on the organizational effectiveness and/or corporate competitiveness have been widely recognized and discussed among both the incumbent managerial and the academicians of economics and sociology in recent years. The purpose of this study is to review the functions of the corporate culture and to examine the factors of the corporate culture among the travel agencies.The present study starts with a conceptual review of the corporate culture. The characteristics of corporate culture is shaped usually with the two major elements; the styles of management and decision-making of the top managerial and the subculture of the employees. Each of the four components of Corporate culture is operationalized as follows; ① tough-guy, macho culture, ② work hard, play hard culture, ③ bet-your-company culture, ④ process culture. These four components of corporate culture become the independent variables while the recognition of environment becomes the dependent variables in the analytical model proposed in this paper. The recognition of environment such as danger and feed-back speed is divided into pre-IMF and post-IMF.Some results of the findings are as follows: First, before IMF, as the danger is recognized low and feed-back speed fast, the corporate culture is shown as work hard, play hard culture. Second, after IMF, as the danger is recognized high and feed-back speed fast, the corporate culture is shown as tough-guy, macho culture.

추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 1종의 Sterol과 3종의 배당체 화합물의 단리·동정

이유건(Yu Geon Lee1),조정용(Jeong-Yong Cho),이현주(Hyun Joo Lee),이용현(Yong Hyun Lee),이상현(Sang-Hyun Lee),한태호(Tae-Ho Han),김월수(Wol-Soo Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5

본 연구에서는 배의 유용성 증명을 위한 일환으로 배의 화학성분을 분자수준에서 밝히고자 하였다. 이에 배 과피 MeOH 추출물을 용매분획하여 얻은 EtOAc-산성 분획과 EtOAc-중성분획을 대상으로 Sephadex LH-20, silica gel, 그리고 ODS colmn chromatography와 HPLC를 이용하여 정제 및 단리하였다. 그 결과, EtOAc-산성 분획과 EtOAc-중성 분획으로부터 각각 2종씩의 화합물을 단리하였다. 단리된 화합물 1-4는 1H- 및 13C-NMR 분석을 통하여 각각 (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (화합물 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone(piceoside, 화합물 2), β-sitosterol (화합물 3), 그리고 β-sitosteryl 3-O-β-D-glucopyranoside (화합물 4)로 동정되었다. 단리된 3종의 배당체 화합물(화합물 1, 2, 4)들은 본 연구에 의해 배로부터 처음 동정되었으며, 화합물 3은 추황배로부터 처음 동정되었다. 본 연구결과가 배 함유 성분연구는 물론 배의 기능성 해명 연구에도 추후 중요한 기초자료로 활용되길 기대한다. We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.

지난 2년간의 학회지 "신경정신의학" 심사 평가보고

김찬형,김세주,김도관,김성곤,박원명,송동호,박용천,이영문,오강섭,정영철,이상열,김주한,이동영,이헌정,함병주,김율리,김문두,김정진,이강욱,천근아,김재진,홍진표,홍승철 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.6

淋菌의 藥劑耐性樣相에 對한 調査硏究

李承薰,石鍾聲,朴蕙瓊,金景俊,柳在根,李龍雨 國立保健硏究院 1973 국립보건연구원보 Vol.10 No.-

전해응집공정을 이용한 염색폐수의 처리

이용택,한승우,조영개,이현문,김태근,손인식,양병수 한국환경과학회 2000 한국환경과학회지 Vol.9 No.4

This research studied the characteristics and applicability of electrocoagulation using aluminium electrode for the color and COD removal in textile wastewater. Electrocoagulation reactor used two different electrode, Fe and Al, since in the general chemical wastewater treatment, aluminium and ferrous salts were used as coagulants. Aluminium electrode showed higher removal efficiency of color and COD than ferrous electrode did. The COD and color removal efficiency improved at the 0.192A/dm^2 current density. Thus, the electrocoagulation process with bipolar aluminium electrode showed better efficiency in the decolorization and COD removal rate of textile wastewater effluent than custom coagulants did.

심미충전재의 적층에 따른 색좌표에 관한 연구

이용근,이성재 대한치과기재학회 1997 대한치과재료학회지 Vol.24 No.2

When filling teeth with light cured composite resins, shade matching can be effectively accomplished by layering. The darker shade filling material should be placed first and then overlaid with the more translucent or lighter shade filling material. In the sandwich technique, which uses glass ionomer base of liner and composite resin, the same optical principles can be applied. Shade matching is one of the important steps in an esthetic filling procedure. Using the incremental layering technique, more life-like esthetic vitality characterization is possible. However, the resultant shade of filled materials is not predictable simply by the color matching with a shade guide. Most layering procedures are performed by experience rather than prescribed scientific procedure. In this study, multiple regression analyses among each color coordinates(CIE ??, ??, ??) of layered filling material and those of upper and lower part constituent materials were performed. Two kinds of light cured composite resins and two kinds of resin-modified glass ionomers of each 5~6 shades were studied. Specimens of 1mm thickness were made, and the color coordinates of each material were measured with the Colorimeter(Model TC-6FX, Tokyo Denshoku Co., Japan). After layering 2 dependant variable, and six of the color coordinates before layering(CIE ??, ??, ??, of lower and upper part material) were used as independent variables in forward regression analysis. The analyses were performed by the grouping of composite resin layering, resin-modified glass ionomer layering, or the simulated sandwich technique. From this experiment, the following results were obtained : 1. The multiple R value of the CIE ?? after layering by the color coordinates before layering was different depending on the type and combination of the materals, and 0.852~0.986. The CIE ?? after layering was closely correlated with the ?? value of upper part material, and the correlation coefficient was 0.765~0.602(p<0.05) 2. The multiple R value of the CIE ?? after layering by the color coordinates before layering was different depending on the type and combination of the materials, and was 0.548~0.879. The CIE ?? after layering was mainly influenced by the ?? value of upper part material, and the correlation coefficient was 0.297~0.602(p<0.05). 3. The multiple R value of the CIE ?? after layering by the color coordinates before layering was different depending on the type and combination of the materials, and was 0.317~0.460(p<0.05). However, the color coordinate of before layering mainly affecting the CIE ?? after layering was different depending on the combination of material. 4. The multiple R value of CIE ?? after layering by the color coordinates before layering was higher than those of ?? ro ?? after layering

열중합과 열순환에 따른 치과용 콤포짓트레진의 상아질 결합강도와 표면특성

이용근 대한치과기재학회 1998 대한치과재료학회지 Vol.25 No.1

The mechanical properties of composite restorations are improved when the composite is free of voids, and the resin matrix is fully polymerized. Making dense, well-cured composite resin restoration is best accomplished by additional heat curing. The purpose of this study was to measure the effect of heat curing or thermocycling of composite resins on the Vickers hardness, color and bond strength to dentin surface. In bond strength study, the effects of other variables such as type of cement and silane treatment were also determined. Two kinds of composite resin(VFP-direct filling composite resin. CRI-composite resin for inlay) were studied. Half of the specimens for hardness and color tests were only light cured and the others were light and heat cured. Vickers hardness and color(CIE L*, A*, B* values) were measured using Microhardness testers(Mitsuzawa Seiki Co., Japan) and Differential Colorimeter(Model TC-6FX, Tokyo Denshoku Co., Japan) before thermocycling, and after thermocycling between 5~55℃ for 500, 1,000 and 2,000 cycles. The composite resin specimen for bond strength test was 5mm in diameter and 6 mm in height. Half of the specimens were only light cured and the others were light and heat cured. These specimens of not-thermocycled and 1,000 thermocycled were bonded to the extracted dentin surface with or without silane application. Two kinds of cement(PAN-adhesive resin cement, FDT-resin modified glass ionomer cement) were used in bond strength to dentin surface test. After 24 hours, the shear bond strength was measured using Universal Testing Machine(Zwick 1456, Z020, Germany) with the cross-head speed of 0.5 mm/min. From this study, the following results were obtained: 1. The Vickers hardness of VFP was significantly higher after additional heat curing than that of only light cured specimen(p<0.05), however that of CRI showed a little change after additional heat curing. The Vickers hardness of VFP decreased significantly after thermocycling(p<0.05), however that of CRI did not changed significantly. 2. The color difference(CIEΔE) value of VFP between not-thermocycled specimen and thermocycled specimen was 3.105∼8.070, and that of CRI was 3.157∼8.752. These color changes were visually perceivable. 3. The color difference (CIEΔE) value of VFP between only light cured specimen and light and heat cured specimen was 2.725∼3.934, and that of CRI was 2.879∼6.143. These results indicated that additional heat curing changed the color of composite resins clinically perceivable. 4. The shear bond strength of VFP to the extracted human dentin surface was 6.353∼14.168 MPa. Additional heat curing did not influenced the bond strength(p>0.05), however the bond strength of thermocycled composite resin specimen was generally lower than that of not-thermocycled. 5. The shear bond strength of CRI to the extracted human dentin surface was 6. 789∼14.790 MPa. In some cases using PAN as the cement, additional heat curing influenced the bond strength(p<0.05). 6. The shear bond strength was influenced significantly by the variables of composite resin, cement and thermocycling (p<0.05).

비귀금속 합금의 표면 처리와 접착제에 따른 상아질의 결합특성

이용근,이성재 대한치과기재학회 1998 대한치과재료학회지 Vol.25 No.2

Many factors can influence on the bond characteristics between the alloys for dental appliances and teeth. Primary factors that can influence on the bonding is alloy type, alloy surface conditions including the surface roughness, bond enhancing primers and the type of adhesives. Many researches were performed on the chemical bonding mechanism of adhesive resin cement, however, micromechanical retention mechanism is basically used. Sandblasting method is widely used to make alloy surface rough, and factors such as the type of blasting particles(alumina or glass), blasting pressure, blasting time and the distance between the nozzle and the target can change the alloy surface conditions after blasting. Repeated casting of dental base metal alloys can result in the change of casting body by the vaporization of lower melting point element metal, and these changes can influence on the bonding characteristics of this casting body with chemically bonding adhesives. The purpose of this study was to determine the shear bonding characteristics of a dental cobalt-chromium alloy to the dentine surface after various alloy surface treatment with four kinds of adhesives. The alloys for bonding test were divided into two groups, one cast 1∼3 times, and the other was cast 5∼7 times. Three kinds of surface treatment of alloys were performed, polished with #600 sandpaper, blasted with 50 micron alumina particles or 50 micron glass particles. These alloy specimens were bonded to human dentine with adhesives of resin-modified glass ionomer cement(FJT), glass ionomer cement(GIC), polycarboxylate cement(HCB) and adhesive resin cement(PAN). The effects of ultrasonic cleaning of alloy specimens after blasting were also determined. Shear bonding characteristics were measured after storage in 37℃ distilled water for 24 hours after bonding. Universal testing machine(Zwick 1456 Z020, Germany) was used, and the cross-head speed was 0.5 mm/min. From the load-deflection curves obtained, shear bond strength, strain at bond failure, work up to bond failure and elastic modulus ratio was calculated. Elastic modulus ratio was the ratio of the slope of the linear portion of load-deflection curve to that of the PAN bonded, alumina blasted and ultrasonic cleaned group. From this experiment, the following results were obtained. 1. The shear bond strength of the group of polished with #600 sandpaper was 5.143∼11.698 Mpa, that of alumina blasted group was 5.888∼17.609 Mpa, and that of glass blasted group was 2.997∼10.084 Mpa. 2. In the FJT bonded group, regardless of the number of casting, the shear bond strength of alumina blasted group was significantly higher than that of sandpaper polished group. However in each of the GIC, HCB and PAN bonded group, the shear bond strength of sandpaper polished group and that of alumina blasted group was not significantly different. 3. Regardless of the number of casting and cement type, there was no significant difference in the bond strength between the sandpaper polished group and glass blasted group. 4. The shear bond strength of alumina blasted group bonded with the adhesive of FJT or PAN showed significant difference depending on the number of casting.

방사선 불투과성 치과용 아크릴릭 레진의 개발

이용근,이건일,이성재 대한치과기재학회 1997 대한치과재료학회지 Vol.24 No.2

Aspiration or ingestions of fragments of dental appliances are common occurrences. If they have proper radiopacity, their localization in and progress through the gastrointestinal tract or trachea can be more effective. To make radiopaque dental acrylic resin, many radiopaque materials, such as iodide compound, magnesium oxide, silver alloy, bismuth compound or barium compound were added into dental carylic resins. However the results were not so satisfactory as to be used clinically. The purpose of this study was to develop a radiopaque dental acrylic resin, which has clinically detectible radiopacity with minimal changes of mechanical and esthetic properties. The radiopacity, color change(CIE ΔE), transverse strength, modulus of rupture and Vickers hardness of acrylic resins were determined after mixing radiopaque glass(SiO₂ 50%, BaO 30%, B₂O₃ 10% and Al₂O₃ 10%) of 10∼50wt%, silane coated radiopaque glass of 10∼50 wt%, triphenyl bismuth of 5∼20% or titanium oxide of 10%. The specimens were thermocycled in 5 and 55℃ distilled water for 1,000 times, after then the radiopacity, color change and Vickers hardness were re-measured. The radiopacity of specimens on the X-ray films was measured with Photoshop program with a film scanner. The color change was determined with differential colorimeter(Model TC-6FX, Tokyo Denshoku, Japan). and the Vickers hardness was measure with microhardness tester(Mitsuzawa, Japan). The flexural strength and modulus of rupture were deterined using the Universal Testing Machine(Zwick 1456, Z020, Germany) with the cross head speed of 5mm/min. The following results were obtained : 1. After mixing silane coated radiopaque glass about 30 wt%, the radiopacity and modulus of rupture increased significant(p<0.05), however the flexural strength and Vickers hardness did not changed significantly(p>0.05) compared to unmodified dental acrylic resins. So, this compost to unmodified dental acrylic resins. So, this composition can be used as a successful radiopaque dental acrylic resin. 2. After mixing radiopaque glass, the flexural strength decreased significantly as the mixing ratio of glass increased(p<0.05). Also the Vickers hardness decreased after mixing in the case of heat cured duntal acrylic resins. 3. After mixing triphenyl bismuth, the flexural strength and Vickers hardness decreased significantly in the case of heat cured duntal acrylic resins(p<0.05). 4. After mixing titanium dioxide, the radiopacity increased significantly(p<0.05), and mechanical properties did not change significantly(p>0.05). However, translucency decreased definitely.