해수에서 분리한 Vibrio sp. M-96 균주의 열감수성 alkaline phosphatase 성질

박문경,진덕희,김중균,공인수,김광현,홍용기 동의대학교 기초과학연구소 1997 基礎科學硏究論文集 Vol.7 No.1

A thermolabile alkaline phosphatase has been purified through steps of osmotic shock, ammonium sulfate salting-out, and DEAE-cellulose chromatography from the cultured broth of the marine Vibrio sp. M-96 strain. The optimal temperature for the enzyme activity was 35℃. The optimal pH was pH 11.0, and the range of pH stability was pH 10.4 to 12.0. Thermal inactivation occured within 6 minutes at 60℃. The enzyme was considerably inactivated by 0.1mM concentrations of Hg^2+, Ni^2+ and Zn^2+, whereas activated up to 234% by 1mM of Mn^2+. The activation energy and deactivation energy by the Arrhenius equation were 4.02 Kcal/mol and 9.09 Kcal/mol, respectively. The Km and Vmax values of the enzyme for p-nitrophenylphosphate were found to be 0.0465mM and 0.001335mM/min. respectively. Active from of the enzyme had a molecular weight of 57,000 dalton determined by the Sephadex G-200 gel filtration method.

제2형 당뇨병 환자에서 Voglibose와 Acarbose의 비교임상연구

정인경,정재훈,민용기,이명식,이문규,김광원,정윤이,박중열,홍성관,이기업 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.26 No.2

연구배경:아카보스와 보글리보스는 ­glucosidase inhibitors로써 비록 약리학적 작용이나 부작용에 있어서 두 약물간에 차이가 있다는 것은 잘 알려져 있으나 당뇨병 환자를 대상으로 아직 이에 대한 두 약물간에 직접적인 비교에 대해 연구된 바는 없었다. 이에 저자등은 국내 2형 당뇨병 환자에 대해 유효성과 부작용 발현에 대해 두 약제를 비교하고자 무작위법에 의한 위약 대조군의 이중 맹검법 연구를 시행하였다. 방법:시험 약제 투여 4주간의 관찰기를 설정하여 공복혈당의 변화가 30㎎/dL 이하이고, 식후 혈당이 200㎎/dL 이상인 환자로 기타 제외 기준에 해당하지 않은 환자 53명을 대상으로 하여 보글리보스 군(24명)과 아카보스 군(29명)으로 무작위로 나누었다. 치료기간은 총 8주로 하였으며, 4주간 간격으로 혈청학적 검사와 부작용을 분석하여 치료 효과가 부작용을 평가하였다. 결과:1)혈당 변화:보글리보스군은 식후 1시간 혈당이 치료 후 4주, 8주째 의미 있게 감소하였도, 아카보스군은 식후 1시간과 2시간 혈당이 치료 후 4주, 8주째 의미있게 감소하였다. 또한 관찰기 혈당에 대한 치료 4주째 감소량은 아카보스군에서 더 큰 경향을 보였으나, 치료 8주째에는 두 군 간의 강하정도에 의미있는 차이가 없었다(p=0.569). 2)인슐린 치의 변화:보글리스 군은 식후 1시간 인슐린 치가 치료 전에 비해 치료 4주, 8주째 감소하는 경향을 보였고, 공복 인슐린이나 식후 2시간 인슐린치는 치료전 후에 의미 있는 차이가 없었다. 아카보스군에서는 치료 전후로 공복 인슐린, 식후 1시간과 2시간 인슐린치에 의미 있는 차이를 보이지 않았다. 두약제 간에 치료 전과 치료 8주사이의 식후 2시간 인슐린의 감소량이 보글리보스 군에서 의미있게 높았다(p=0.040). 3)당화혈색소:보글리보스 군은 치료 전에 비해 치료후 당화혈색소가 감소하는 경향을 보였고, 아카보스군은 치료 전에 비해 의미있게 감소하였다. 당화혈색소 변화량은 두 군간에 의미 있는 차이는 없었다(p=0.412). 4)지질대사의 변화:중성지방, 콜레스테롤, 고밀도 진단백 콜레스테롤에 대해 두 군간에 의미 있는 차이는 없었다. 5)부작용:소화기계 부작용의 빈도는 치료 4주째 보글리보스 군에서 의미 있게 낮았으나(p=0.028), 치료 8주째 부작용의 빈도는 두 군간에 의미 있는 차이가 없었다(p=0.215). 결론:2형 당뇨병 환자에서 보글리보스와 아카보스의 두 약제의 임상적 유효성과 부작용발현에 대해 비교한 결과 치료 후 8 주 후 혈당강화효과는 두 약제간에 유사한 효과를 보였으나 보글리보스군에서 4주째의 초기 위장관 부작용이 적었다. Background : Acarbose and voglibose are alpha-glucosidase inhibitors. Although different pharmacological effects and adverse abdominal events associated with the two drugs have been reported, no study directly compared acarbose and voglibose in diabetes has been undertaken. To compare the pharmacological effects and gastrointestinal adverse events between two drugs, a randomized, placebo-controlled, double-bind study was performed in type 2 diabetes patients. Methods : The period of study was 12 weeks(observation period: 4 weeks; treatment period: 8 weeks). Fifty-three patients were randomized into two groups(the acarbose group: 24 patients; the voglibose group: 29 patients). The serum glucose, insulin, fructosamine, HbA_1c, cholesterol, triglyceride and the incidence of adverse events were measured. Results : 1) The reduction of glucose from before treatment to 4 weeks after treatment was significantly higher in the acarbose group, but the change before treatment and 8 weeks after treatment in the two groups was similar(p=0.569). 2) The insulin significantly decreased after voglibose treatment(p=0.040). 3) HbAa_1c level tended to decrease in voglibose group, and there was a significant decrease after acarbose treatment. However, the change in HbA_1c level before and after treatment was similar between the two groups(p=0.412). 4) The two drugs did not cause any other changes in the total, HDL-cholesterol and triglyceride. 5) The number of patients with gastrointestinal adverse events was significantly low 4 weeks after voglibose treatment (p=0.049), but the incidence in the two groups was similar after 8 weeks(p=0.215). Conclusions : Acarbose and voglibose significantly improved postprandial hyperglycemia in diabetes. The incidence of gastrointestinal adverse events was low 4 weeks after voglibose treatment(J Kor Diabetes 26:134~145, 2002).

부산지역 외상성 척수손상환자 발생의 변화추이

강경문,김기찬,정호중 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Objective: To identify trends in the epidemiologic and injury data of person with traumatic spinal cord injury (SCI). Method: Review of medical records of 312 patients with traumatic spinal cord injury from 1996 to 2005 at the four university hospitals and one training hospital in Busan was performed. The epidemiologic informations of this study were compared to those of the past study conducted between 1986-1993 and 1996-2005. Results: Average age(42.8) at injury was rised. Male-to-female ratio was 3.5:1, the population of males has decreased significantly in recent years(1996-2005). The leading cause of traumatic SCI was traffic accident. Tetraplegia accounted for 52.2% of all traumatic SCI, 47.8% were paraplegia. Complete injury decreased compared to the past occupying 41% of all the injury while tetraplegia increased. Traumatic spinal cord injury occured most commonly in August, summer, the time of 6 A.M to midday and on Saturday. Conclusion: This study showing the recent characteristics of traumatic SCI in Busan revealed increase in the average age at injury, the rate of females and incomplete injury. The most frequent time of onset was from 6 AM to midday.

자동 형상제어용 측정롤의 특성에 관한 연구

전언찬,김중완,김순경,김문경 동아대학교 공과대학부설 생산기술연구소 1996 生産技術硏究所硏究論文集 Vol.1 No.2

The continuing demand for quality products requires better understanding and improved control of the production process. And, in recent year requirements for flatness control in strip rolling have become increasingly severe because of the control for flatness of cold rolled strip is essential for further down stream processing. Also a speeds of rolling mills to meet productivity requirements puts a demanding requirement on the control of flatness of rolled strip. The demands on a total flatness control system therefore are a measuring and indication system consisting of a measuring roll that is robust, accurate, reliable and require a minimum of maintenance. The critical part of any control system is the quality of the information being provided by measurement instrument or device. It is therefore of utmost importance to have an accurate, repeatable measuring system.

CW Laser Annealing 과정중 SOS 상의 시간적 온도변화 측정에 관한 연구

고년규,윤희중,유동선,최원국,문경순 연세대학교 대학원 1987 延世論叢 Vol.23 No.1

The purpose of this experiment is to demonstrate the new method of TSRTM during CW Ar laser annealing using a He-Ne laser probe beam. Optical interference phenomena by a small changes of refractive index of Si, which is induced by the temperature change during short time laser annealing on the Si wafer surface, was used. The samples, SOS(Silicon on Sapphire), used in this work were epi. <100> Si grown on a <1012> insulator sapphire substrate with 2.0μm thickness. From the experimental temperature dependent equation of the refraction of index of Si with respect to the probe beam(λ=6328 Å) in furnace experiment, n(T)=3.98+7×10-4T (℃), the temperature on the destructtve or constructive interference was calculated. Incident Ar laser beam whose power ranges from 1.8W to 2.8W was irradiated and the effect of temperature rise of Si wafer on the elliptical spot whose major and minor axis is 200μm, 15μm respectively is investigated by the He-Ne laser probe beam which is focused 30μm. The annealing time ranges from 8 msec~250 msec to 500 msec~20 sec was adopted to estimate that the first constructive interference is occurred at least total irradiated power density 5.46×102Jㆍsec/cm2, and it is inferred that more than 180msec is needed for the first constructive interference when the total incident energy is 2.8 W. For the longtime annealing, about more than 1 sec, the interference pattern was coincided with the result produced by the furnace experiment. It is investigated from the space-resolved temperature gradient that the irradiated laser beam was focused in center.

강판의 자동 형상제어 장치의 특성에 관한 연구

전언찬,김중완,김문경,김순경 동아대학교 공과대학부설 생산기술연구소 1996 生産技術硏究所硏究論文集 Vol.1 No.2

In this paper, the performance and functions of automatic flatness control system installed on the 4 hi-reversing mill and has been investigated under actual conditions. A new automatic flatness control system incorporates a measuring roll for measurement and correction calculations, hydraulic roll benders, selective roll cooling, and a programmable controller forinterface and data logging. The test results are as following. The more the exit steel strip thickness is thick, the smaller the I value, and the more it is thin, the larger the I value. And, a complex distribution of strip tension was controlled, for example, not only a quarter buckle but also a simple center wave and edge wave. Because the tension deviation is larger at acceleration speed and deceleration speed than steady speed. so automatic flatness control system of contact type is better to adopt over 450 m/min, automatic flatness control system reduces rapidly large flatness deviation. The maximum I value of strip has been decreased to 13 I, and defects caused by poor flatness have been drastcally decreased. And coolant temperature for work roll cooling system on the automatic flatness control system is better to adopt about 50-55℃.

심초음파로 결정된 응급 심낭천자술의 천자부위

김성환,황성오,이강현,조준휘,강구현,문중범,이승환,윤정한,최경훈,김영식 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background: The aim of this study was to determine whether the conventional subcostal approach is suitable for emergency pericardiocentesis in patients with cardiac tamponade or impending cardiac tamponade. Methods: This study was a prospective, observational study conducted at the emergency department of a tertiary hospital, Patients who had symptomatic pericardial effusion and who needed emergency pericardiocentesis in the emergency department were included in this study. We measured the epicardium-to-pericardium distance at the subcostal, parasternal, and apical area with two-dimensional echocardiography to determine the appropriate puncture site for pericardiocentesis. An epicardium-to-pericardium distance of more than 1.0 cm was considered as the primary safety factor in determining the Puncture site for pericardiocentesis. The skin-to-pericardium distance was considered as secondary safety factor. Results: Ninety-five consecutive patients(55 males and 40 females; total mean age: 53 year old) with cardiac tamponade or impending cardiac tamponade were enrolled in this study. The puncture site for pericardiocentesis, as determined by echocardiography, was the subcostal area in 43 patients(45%), the apical area in 40 patients(42%), the left parasternal area In 11 patients(12%), and the right parastemal area in one patient(1%). Pericardiocentesis failed in 2 patients(2%) with the subcostal approach and in one patient(1%) with the apical approach. The average epicardium-to-pericardium distance was 31 ±21 mm in patients with the subcostal approach and 21±8 mm in patients with other approaches. There were no differences in the amount of pericardial fluid and in the intraperical pressure among patient groups according to puncture site. There were two procedure related complications: a puncture of the right ventricle with the subcostal approach and a ventricular tachycardia with the apical approach.

이중혈류유발 심폐소생술이 심정지를 유발한 개의 단기 생존율에 미치는 영향

황성오,조준휘,강구현,김성환,문중범,이강현,이승환,윤정한,최경훈,홍은석 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background and Objectives: We previously reported that, compared with standard cardiopulmonary resuscitation(S-CPR), better hemodynamic effects could be achieved by simultaneous sterno-thoracic cardiopulmonary resuscitation(SST-CPR) in which we compressed the sternum and constricted the thorax circumferentially during the systolic period by using a device. This study was designed to assess whether SST-CPR, compared with S-CPR, improve the survival rate of dogs with cardiac arrest. Subjects and methods: Twenty-five mongrel dogs(19∼31㎏) were enrolled in this study. After four minutes of ventricular fibrillation induced by an AC current, animals were randomized to resuscitate with either S-CPR(n=13) or SST-CPR(n=12). Epinephrine(1 ㎎) was injected into the right atrium every three minutes after the beginning of CPR. Defibrillation was attempted after 6 minutes of CPR. Standard advanced cardiac life support was started if defibrillation was not successful. Results: SST-CPR resulted in significantly(p<0.001) higher systolic arterial pressure(91±47 vs 47±24 ㎜ Hg), diastolic pressure(43±24 vs 17±10 ㎜ Hg), coronary perfusion pressure(35±25 vs 13±9㎜ Hg), and end tidal CO2 tension(9±4 vs 3±2 ㎜ Hg). Two of 13 animals(15 %) resuscitated with S-CPR and six of 12 animals(50%) resuscitated with SST-CPR survived until 12 hours after cardiac arrest(p<0.05). Donclusion: SST-CPR, compared with S-CPR, improves the short-term survival rate in canine cardiac arrest.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

Mechanisms of Topographic Development on Ni Surface by Reactive Ion Beam Sputtering

Kyung Joong Kim,Dae Won Moon,Kyung-Hoon Jung 한국진공학회 1997 한국진공학회 학술발표회초록집 Vol.- No.-