발효조를 이용한 Monascus anka의 적색소와 황색소의 생산

강성국,임종환,정순택,김선재 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

Monascus anka를 이용하여 천연식용색소를 대량생산하기 위한 방법을 개발하기 위하여 발효조를 이용하여 적색소와 황색소의 색소생산의 최적 배양 조건과 생산된 색소의 색깔 특성을 조사하였다. Monascus anka 적색소와 황색소를 생산하였는데, 이들 색소는 균체내색소와 균체외 색소로 구분되었다. 균체의 적색소(ERP)는 494nm에서, 균체외 황색소(EYP)는 380nm에서, 균체내 적색소(IRP)는 506nm에서 그리고 균체내 황색소(IYP)는 388nm에서 최대 흡광도를 나타냈다. 적색소와 황색소 생산, 색조의 특성 및 균체증식력을 배양 온도, pH, 쌀가루 농도, peptone 농도, magnesium sulfate 농도, 통기량 및 교반속도에 대하여 조사한 결과 적색소 생산력은 30℃, 초기 pH 6.0, 쌀가루 농도 3~5%, peptone 농도 0.05%, magnesium sulfate 농도 0.25%, 통기량 0.1vvm 교반속도 300rpm의 조건에서 최대치를 보였으며, 이때 ERP, EYP, IRP 및 IYP는 각각 A_494nm 0.84, A_380 nm 0.71, A_506 nm 1.18, A_388 nm 1.10, L값은 각각 29.40, 6.44, 34,98, 8.94, a값은 각각 5.76, -1.08, 11.83, -1.55, b값은 각각 18-19, 3.41 27.26, 6.16 그리고 균체량은 7.4 g/l을 보였다. 또한 황색소 생산력은 온도의 경우 적색소 생산을 위한 최적온도 보다 높은 35℃ 부근에서 우수하였으며 초기 pH와 쌀가루 농도는 적색소 생산을 위한 최적조건과 같았고, 질소원과 무기염의 농도가 높을수록 그리고 통기량이 많을수록 황색소 생산력이 우수하였다. 적색소와 황색소 생산을 위한 발효조의 교반속도는 100~300 rpm이 적합하였다. Production of Red and Yellow Pigments from Monascus anda in a Jar Fermenter, Seong-Gook Kang, Jong-Whan Rhim, Soon-Teck Jung^* and Sun-Jae Kim. Department of Food Engineering. Mokpo National University Chonnam, 534-729, Korea - In order to develop the method for mass production of natural food colorant from Monascus anka, optimum cultivation conditions for producing red and yellow pigments by cultivating the mold in a jar fermenter and their color characteristics were investigated. The mold produced red and yellow pigments both intracellularly and extracellularly. These pigments showed unique light absorption characteristics with maximum absorption of 494, 380, 506, and 388 nm for extracellular red pigment (ERP), extracellular yellow pigment (EYP), intracellular red pigment (IRP), and intracellular yellow pigment (IYP), respectively. Optimum conditions for producing red pigments were found to be temperature 30℃, initial pH 6.0, rice powder 3~5%, peptone 0.05%, magnesium sulfate 0.25%, aeration rate 0.1vvm. Optimum temperature for producing yellow pigments was around 35℃ which is higher than that of producing red pigments. The initial pH rice powder concentration for producing yellow pigments were the same as those of producing red pigments. The higher concentration of nitrogen source and inorganic salt, aeration rate, the more the yellow pigments were produced. Them optimum agitation speed was 100~300 rpm for pigment production.

신이식에서 이식신의 생존분석

오선미,김종학,황평주,구영선,강민규,나기량,김종섭,김성숙,이강욱,신영태,설종구,배진선,손기섭 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

To investigate the prognostic factors for the survival of transplanted kidney in patients with end-stage renal failure, 59 cases of renal transplantation from September 1986 to Feburary 1997 in Chungnam National University Hospital were analyzed retrospectively. The results were as follows: 1)The mean age of recipients was 33.8 years and that of doners was 38.9 years. The male to female ratio of recipients was 2.5:1, and that of donors was 1.03:1 2) Living related donore(LRD) were 79.6% and living non-related donors were 20.3%. The HLA-identical donors(ID) in LRD were 18.7% and HLA-haploidentical donors(HID) were 61.0%. In living non-related donors(LNRD), mean matched HLA-AB antigens were 1.56 and mean matched HLA-DR antigens were 0.56. 3) The average 5-year patient survival fate was 94%, and average 5-year graft survival rate was 70%. The 5-year graft survival fate of HLA-ID was 100%, and those HLA-HID and LNR were 70% and 36% respectively. 4) Total 33 episodes of acute rejection were found in 45.8% of transplanted patients. The number of acute rejection episode did not show significant difference between LRD and LNRD(P > 0.05). There was no significant difference in MLC between two groups(p > 0.05). 5) In comparison between the 18 patients who lost their graft function in 5 years and 17 patients who are maintaining graft function for more than 5 years, MLC was significantly lower in patients with functioning graft than of patients with non-functioning graft (p < 0.05). The number of rejection episode was also lower in patients with functioning graft than that of the patients with non-functioning graft(p < 0.05). However, there were no significant difference in recepient and donor age and history of pre-transplantation donor specific transfusion between two groups. With the results above, we can speculate that adequate donor selection according to good matched HLA typing and low MLC is very important for graft survival in renal transplantation. Prevention and treatment of acute rejection in renal transplantation is also na important factor for graft survival.

판막 질환에서의 심근 섬유화 : 심근 조직을 이용한 형태학적 연구 및 혈청 생화학적 지표의 분석

강수진,박덕우,송재관,박경민,송종민,강덕현,한기훈,송현,이재원,주석중,송명근,김정선 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

사회과에서 인터넷 활용수업이 네트웍 리터러시 함양에 미치는 효과

강운선,김종혜 대구대학교 2006 대구대학교 학술논문집 Vol.1 No.2

이 연구의 목적은 인터넷 활용수업이 중학생들의 네트웤 리터러시 함양에 미치는 효과를 밝히는 것이다. 본 연구에서는 인터넷 활용수업의 방법으로 온라인 카페를 활용한 수업을 진행하였으며, 인터넷 활용수업을 적용한 학습자 집단의 네트웤 리터러시 점수가 전통적인 강의식 수업을 활용한 학습자 집단보다 높다는 가설을 설정하였다. 이를 검증하기 위하여 대구의 능인중학교 1학년 학생을 연구대상으로 선정하였다. 연구대상을 실험집단과 통제집단으로 구분하고 교사변인과 수업내용을 통제하기 위하여 동질성을 확보하였다. 지능과 사회경제적 지위가 학업성취도에 영향을 미칠 가능성을 통제하기 위하여 지능과 사회경제적 지위를 통제변인으로 선정하고 수업방법을 가변수로 처리하여 회귀분석을 실시하였다. 분석결과 인터넷 활용수업을 적용한 집단의 네트웤 리터러시 점수가 전통적인 강의식 수업을 적용한 학습자 집단보다 평균점수가 4.48보다 높게 나타났으며, 그 차이는 통계적으로 유의하였다. This study had an object to present the way of internet in education in sociology, and to prove the effect of internet in education on network literacy cultivation. In relation to this study, internet in education and traditional teaching method were carried out in every class. After that, this study proved the difference of two lessons in network literacy cultivation. For the purpose of this study, a hypothesis was established as follows; Internet in education group will get higher marks than traditional teaching method group in evaluation marks of network literacy. To verify this hypothesis, experimentation was carried out by selecting two classes of boys' middle school in Daegu and they were divided into experiment group and control group. The two groups selected as a sample group were treated according to the following procedure. This study applied the value of Cronbach α to verify trust-degree in a means of measurement, and used multiple regression analysis to analyze the opposite effect in methods of classes. As a result of this study, the group of internet in education got higher marks so much as 4.884 than the group of traditional teaching method for evaluation marks of network literacy. Accordingly, the hypothesis of this study was selected and this study could conclude internet in education was a effective method upon network literacy cultivation by increasing access, analysis, evaluation, mental capacity in information.

이모장치 착용 후 하악 이부의 형태변화

강선,박동철,김정기 대한치과교정학회 2000 대한치과교정학회지 Vol.30 No.1

성장기 아동에서의 골격성 Ⅲ급 부정교합을 치료하기 위하여 사용되는 이모장치는 하악골의 후하방 회전 또는 하악골 길이 성장의 억제에 의하여 하악 전돌감을 감소시키는 것으로 알려져 있다. 그러나 이에 관한 연구는 하악골 성장의 주요 부분을 담당하는 하악 과두부위와 관련된 것이 대부분이었다. 본 연구는 장기간 이모장치를 착용하였을 때 구외력이 직접 적용되는 부위인 하악 이부의 형태 변화를 평가하기 위해 시행하였다. 전북대학교병원 치과교정과에서 교정치료를 받은 혼합치열기 아동 62명 (이모장치군 32명, 대조군 30명)을 대상으로 측모 두부규격 방사선사진을 채득하여(치료 또는 관찰기간 평균 2.1년) 계측치 (각도 4개, 거리 5개, 비율 2개)를 평가하여 다음과 같은 결론을 얻었다. 1.치료 후 symphysis height는 이모장치를 사용하지 않은 군과 이모장치 착용군에서 모두 치료 전보다 증가하였다. symphysis depth는 이모장치를 사용하지 않은 군에서 치료 후 증가하였으나 이모장치 착용군에서는 치료 전과 차이가 없었다. 특히, posterior symphysis depth가 이모장치 착용군과 대조군에서 모두 감소한 반면, anterior symphysis depth는 대조군에서는 증가하였고 이모장치 착용군에서는 치료 후 감소하는 경향을 보였다. 2.chin depth와 chin curvature는 이모장치를 사용하지 않은 군에서는 증가하였으나 이모장치 착용군에서는 치료 후에도 변화를 보이지 않았거나 감소하였다. 대조군에서 chin angle, menton angle, symphysis angle은 감소하는 경향을 보였으나 이모장치 치료군에서는 오히려 증가하였다. 이것은 하악골의 성장에 따라 정상적으로 나타나는 pogonion 부위의 골침착이 이모장치 착용군에서 chincup의 직접 접촉에 의해서 억제된 결과로 생각된다. 3.성장기 아동에서 이모장치를 착용하였을 때 하악 이부의 전방 성장 억제에 의하여 치료 전과 유사한 하악 이부 형태가 유지되었으며 이것은 주로 하악 이부의 전방부의 골침착 억제에 의한 결과인 것으로 생각된다. Although it is well known that the chincup, used to correct a skeletal class III malocclusion in growing children, reduce the mandibular prognathism by arresting the growth of the mandibular length and rotating the mandible posteroinferiorly, the majority of the studies about chincup is focused on condylar head that plays an important role in mandibular growth. The aim of this study was to evaluate the morphologic chance of the mandibular symphysis where extraoral force is applied directly during chincup treatment. The data for this study were obtained from lateral cephalometric radiographs of 62 growing children(chincup group:32, control group:30) with mixed dentition who had been accepted for the orthodontic treatment at Chonbuk National University Dental Hospital. The results were as follows : 1.Symphysis height was increased both in chincup therapy group and control group during treatment. Symphysis depth was decreased or maintained the initial values in chin cup therapy group, whereas increased in control group. Posterior symphysis depth was decreased both in chin cup therapy group and control group, but anterior symphysis detph was increased in control group, whereas decreased in chincup therapy group. 2.Chin depth and chin curvature were increased in control group, whereas maintained or decreased in chincup therapy group during treatment. Chin angle, mentors angle and symphysis angle were decreased in control group, whereas increased in chincup therapy group. It suggested that bone deposition in pogonion area that occur normally with mandibular growth was supressed by direct contact of chincup. 3.When growing children wear chincup, symphysis morphology was maintained due to inhibition of forward growth at mandibular symphysis. It may be due to the suppression of bone deposition in anterior part of symphysis.

성견 경골에서 자가장골이식이 임플란트 골유착에 미치는 영향

강남연,김명래,김선종 이화여자대학교 의과대학 2009 EMJ (Ewha medical journal) Vol.32 No.2

Objectives:This study was performed to evaluate the effect of autogenous iliac particulated marrow cancellous bone graft on osseointegration of implants in the dog tibia which has a loose bone marrow. Methods:Three mongrel dogs, weighing about15kg or more, and RBM(resorbable blasted media) AVANAⓡ implants(Osstem Inc., Korea) were used in this study. In the control groups, 2 implants sites were prepared conventionally with drills in the left metaphysic of tibia and 2 implants were placed in each dog under general anesthesia. In the experimental groups, 2 implant sites were prepared with drills. and the particulated marrow cancellous bone, which was obtained from the right ilium,was packed into both prepared sites with osteotomes in the right metaphysic of tib-ia in each dog. Two implants were placed. A total of 12 implants were placed in 3 dogs. The animals were sacrificed at 8 weeks after implant placement. The specimens were taken and embedded into the resin. H & E stain was done. The undecalcified specimens were prepared for histological examination and histomorphometric analysis of bone-implant contact ratios. The Mann-Whitney test was used to compare both groups.Values of p<0.01were considered statistically significant. Results:In the cross-sectional radiographs of the control group no trabecular bone was observed in the marrow space and the implant was surrounded with radiolucent marrow space. In the experimental group Some radioaque trabecular bone was observed in the marrow space of tibia and some of the trabecula was contacted with implant surface. Histologically,in the control group few trabecular bone was observed in marrow space and there was poor osseointegration in the marrow. In the experimental group most marrow space was filled with the trabecular boneand the implant surface was contacted with trabecular bone. In histomorphomeric analysis the bone-implant contact ratio of the experimental group(75.6%) was significantly higher than that of the control group(27.1%,p<0.01). Conclusion:The autogeneous bone graft into the loose bone marrow can be an option to improve the bone density and to get better osseointegration of dental implants.

신부전증과 폐에 Pseudolyrmphorma를 동만한 원발성 Sjogren 증후군 : Cyclophosphamide와 Prednisolone에 의한 성공적인 치료

강민규,구영선,황평주,김종학,나기량,서광선,이강욱,신영태 대한신장학회 2000 대한신장학회잡지 Vol.19 No.4

부비동 내시경수술 후 비음도 변화

동헌종,김선일,권중근,윤영선,강수미,추광철 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

신경 교차연결 후 흰쥐 골격근섬유의 마이오신 동질형의 변화

이종은,이원택,강유선,박경아 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

발효 온도와 시간 조합을 달리한 총각김치의 품질 특성

강정화,강선희,안은숙,정희종 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.6

To determine the conditions of the fermentation and storage for Chonggak kimchi refrigerator, prepared Chonggak kimchi took into kimchi refrigerators which were controlled at four different modes of the fermented temperature and time, and fermented and kept for 16 weeks. The pH in Chonggak kimchi fermented at 20℃ for 24 hours/stored at 01℃ dropped greater than all of kimchi fermented at other combinations, and the changes of pH at any combinations were not greater than those in Baechu kimchi, because pH in Chonggak kimchi did not dropped below 4.5. Acidities in Chonggak kimchi were greatly increased at higher temperature. The acidity in Chonggak kimchi during the first week of fermentation was lower than that in Baechu kimchi and then it was rather higher becuase of the addition of waxy rice paste. In texture, puncture force of Chonggak kimchi was decreased slowly until 8 weeks of fermentation and then did not changed much and the highest values showed in Chonggak kimchi stored directly at -1℃ without any fermentation. In sensory evaluation, the score for the carbonated flavor and the sourness were the highest in Chonggak kimchi fermented at 20℃ for 24 hours/stored at -1℃, but the lowest in Chonggak kimchi stored directly at -1℃ without any fermentation because of some undesirable flavors. The lowest hardness showed in Chonggak kimchi fermented at highest temperature and the best hardness was in Chonggak kimchi fermented at 5℃ for 3 days or 6 days/ stored at -1℃. The appearance was the best in Chonggak kimchi fermented at 20℃ for 24 hours/stored at -1℃ and the worst was in Chonggak kimchi stored directly at -1℃ without any fermentation. The overall acceptability of Chonggak kimchi fermented at 20℃ for 24 hours/stored at -1℃ was good after 4 weeks of fermentation, but in Chonggak kimchi fermented at 5℃ for 3 days or 6 days/stored at -1℃ it was food after 6 weeks. Total microbial counts in most of Chonggak kimchi were reached to a maximum number within 7 days, and then decreased similarly at all modes. Leuconostoc spp. and Lactobacillus spp. increased to maximum number of 1.48 10^(9) and 5.62 0^(9), respecively, in Chonggak kimchi fermented for 7 days. Yeast counts showed a increasing trend not depends on fermenting temperature and they were lower counts than those in Baschu kimchi. Waxy rice paste which added to Chonggak kimchi resulted in increasement of glucose as a carbon source and stimulated to reproduce the microbes in Chonggak kimchi.