팥 신장기 과습처리에 의한 생육특성 및 단백질 발현

정해룡, 유장환, 윤성현, 권수정, 우선희 충북대학교 농업과학기술연구소 2022 農業科學硏究 Vol.38 No.2

Adzuki bean is sensitive to waterlogging stress. The overall study on the waterlogging stress is limited comparing to the study on the drought and any environmental stress. Therefore, the present study was conducted to investigate the changes in growth characteristics and the expression patterns of proteins at the 5-leaf stage periods of adzuki bean. The domestic cultivar, Arari (Miryang No. 8) was used to test the waterlogging stress. In the waterlogging treatment for 3 days, the plant height showed slightly decrease in the treatment at 3 days of waterlogging, but root fresh weight showed significantly changes at 3 days of the waterlogging treatment. Chlorophyll contents showed also significantly different at 3 days of waterlogging treatment compared to control the plants. The waterlogging stress gradually influenced the growth differences between the control and the treatment respectively. More than 350 pro- tein spots were identified on 2-D gels using an image analysis. Moreover, a total of 28 proteins were analyzed using LTQ-FT-ICR MS. Among these 28 proteins, a total of 18 proteins were up-regulated, and 10 proteins were down-regulated under waterlogging treatment. According to biological process, the most of the proteins were found to be involved in carbohydrate metabolism. Regarding the subcellular local- ization, most of the proteins were localized into chloroplasts in 5-leaf stage.

자동차 허브 내·외경 측정 시스템 개발

윤규택,이동주,최만용,박해원,박정학 한국공작기계학회 2002 한국공작기계학회 춘계학술대회논문집 Vol.2002 No.-

An automobile hub is composed of a hollow cylindrical part with inner and outer diameters. These hub diameters must be precise, as any irregularities in them (diameters) can influence/degrade the motion of an automobile with this problem. Highly accurate and reliable hub inner and outer diameters measurement systems are therefore required for inspection of the diameters. In this research, an automobile hub inner and outer diameters measurement system has been developed and tested. The system consists of measuring heads with LVDT sensors, and associated software for automatic measurement and calibration of the diameters and the sensors respectively. The system has been evaluated in comparison with an existing measurement system, and the following outcomes have been achieved; ·Improved and guaranteed quality and reliability ·Increased productivity and cost reduction These results prove the system developed in this research to be suitable for automobile hub inspection.

측두하악관절장애 환자에서의 평면 골 스캔 및 Bone SPECT 소견과 임상적 및 영상학적 소견과의 비교

정희정,조봉혜,정연화 대한구강악안면방사선학회 2004 Imaging Science in Dentistry Vol.34 No.2

Purpose : This study was performed to evaluate the diagnostic value of both planar and SPECT imagings by comparing the results with the clinical and other imaging modalities findings. Materials and Methods : A total of 578 joints with TMJ complaints were examined using planar scintigraphy and SPECT. The planar scintigraphy and SPECT findings were analyzed and compared to the clinical findings of pain, clicking, crepitus and limitation of mouth opening. Moreover we compared the accuracy of the planar scintigraphy and SPECT methods with the one of the panoramic, transcranial, tomographic and MR imaging methods. Results : The planar scintigraphy and SPECT methods showed a high sensitivity of 0.76-0.84 and low specificity of 0.25-0.45 toward the clinical findings: pain, clicking, crepitus and mouth opening restriction. Simple radionuclide uptake ratio was high in each group of patients with pain, crepitus, limitation of mouth opening (p.0.05) complaints, in each group with positive bone changes on panoramic, transcranial or tomographic images, and in disc displaced group. Conclusion : Although both planar and SPECT imagings have limited specificity, these techniques are sensitive for detection of internal derangement of the TMJ.

직업적 아크릴아미드 폭로에 의한 다발성 신경병증

정해관,권용욱,어경윤,김병준,양정선,정철,진영우,이미영,강성규 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.3

Authors report a first Korean case of polyneuropathy in an acrylamide producing factory together with an environmental and epidemiologic survey for the ten workers of the same workplace and 46 workers in the production and manufacturing acrylamide in Ulsan, Korea. The case is 30 years old man who worked in the production of the acrylamide for two years. In August, 1997, he had a weakness of the lower extremity with loss of body weight of 15 Kg which progressed to paresthesia of the limbs, the loss of vibration sense, difficulty in walking, speech disturbance, dysuria, constipation, difficulty in erection and swallowing difficulty. Neurologic examination showed a complete loss of vibration and position sense, and loss of deep tendon reflex in all the limbs. There was a contact dermatitis-like skin lesion on the feet. Electrodiagnostic test showed a generalized polyneuropathy of moderate degree involving motor and sensory component of peripheral and cranial nerves. After 8 months' cessation from exposure and supportive care, his symptoms were completely resolved, but abnormality in electrodiagnostic test and vibrotactile perception threshold still persisted, although much improved than before. There were 11 workers in the same workplace of the case, producing acrylamide by microorganism as a catalyst. Environmental monitoring of the workplace revealed air borne concentration of the acrylamide to be from 0.05 to 0.23 mg/m3 by personal sampling and from 7.65 to 11.65 mg/m3 by area sampling during filter exchanging process, which far exceeds TLV-TWA of 0.03 mg/m3. However, acrylamide was not detected from the plasma of the workers. Symptom questionnaire showed a markedly higher complain of neurologic symptoms, compared to the workers of the other acrylamide producing companies. Electrodiagnostic test showed a several workers are in the lower normal range without definite abnormality. Vibrotactile perception threshold by Vibratron Ⅱ showed a significantly increased threshold in the workers of the same workplace compared to the other acrylamide workers and significantly decreased test score of color vision compared to normal controls. After improvement of the production process and strict wearing of the protective device, most workers reported the improvement of clinical symptoms, but vibrotactile perception threshold was not improved. These result suggests the need for the effective environmental monitoring together with a periodic biological monitoring. Development of effective screening test is urgently needed to control and assess the skin absorption of acrylamide.

저빈도 반복성 경두개 자기자극이 정상인의 시각적 단기 기억에 미치는 영향

오해정,김원,이경욱,박원명,전태연,한진희,채정호 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.5

Objectives : Repetitive transcranial magnetic stimulation (rTMS) is a novel, noninvasive method of brain stimulation. Since conventional rTMS does not cause seizure, marked advantage over electroconvulsive therapy (ECT), rTMS can be used easily and widely. Once the effects of rTMS on cognitive function are elucidated, its application will be further extended. However, the effects of rTMS on cognitive function have not been well examined. This study aimed to evaluate the effect of low frequencyr TMS on visual short term memory. Methods : Forty normal healthy volunteers were randomly assigned to rTMS (n=20) or sham (n=20) groups. The rTMS group were again randomly divided into left prefrontal (n=10) and right prefrontal (n=10) groups. For each group rTMS was given at 1 Hz for 20 minutes with 100% of motor threshold. As a cognitive task, Corsi Block Tapping Test (CORSI) in the computerized Vienna Test System, which can assess visual short term memory, was performed before, during, and after the rTMS. Results : No significant difference was found in the visual short term memories between the rTMS and the sham groups before, during, or after 1 Hz rTMS, nor between the left and right prefrontal rTMS groups. Conclusion : This study demonstrates that rTMS, contrary to ECT, has no adverse visual short term memory effect when used as the standard treatment paradigm. Therefore, rTMS could be a new and safe treatment method in various neuropsychiathc fields without any interference of cognitive function.

혼합형 자가면역성용혈성빈혈과 동반된 Evans 증후군 1례

선길홍,윤찬영,박상곤,박경희,우정주,한경택,김진화,김영훈,정춘해,박치영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Evans syndrome is defined as a simultaneous or sequential occurrence of autoimmune hemolytic anemia and autoimmune thrombocytopenia. Autoimmune hemolytic anemia (AIHA) is usually induced by IgG warm autoantibody or cold-active IgM antibodies reacting specifically with antigens associated with a patient's RBC. AIHA is a fairly uncommon disorder, with estimates of the incidence at 1 to 3 cases per 100,000 per year. Mixed-type AIHA is a relatively uncommon form of AIHA, with studies noting the incidence of 7-8% among cases of AIHA. We experienced a patient, 46-year-old woman who was diagnosed having a very rare clinical presentation of mixed warm and cold antibody mediated Evans syndrome. She was treated with corticosteroid therapy only and has been maintaining a complete response for 15 weeks. 저자들은 혼합형의 자가면역성 용혈성 빈혈과 자가면역성 혈소판 감소증이 동시에 발생한 혼합형 Evans 증후군으로 진단하고 스테로이드 요법 후 혈액학적으로 회복된 상태로 15주가 지난 현재 steroid 5 mg/일 까지 감량한 상태에서 추적 관찰 치료중인 46세 여자 환자를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.

회맹장 및 대장을 침범한 장 결핵에 있어서 바륨을 이용한 방사선 이중조영촬영 소견에 대한 연구

진용현,전혜정,이용근,강철호,조성범,김윤환,박철민,차상훈,김일영,이혜경 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

To determine the characteristic double contrast barium enema findings of ileo-cecal and colonic tuberculosis and to make differential diagnosis from other bowel inflammatory disease. Sixty-seven patients confirmed as ileo-cecal and/or colonic-tuberculosis in six hospitals were evaluated retrospectively. The diagnosis was proven by endoscopic biopsy, surgery, and clinical follow up. Nine of 67 patients were excluded because radiologic findings were normal though endocopic biopsy examinations resulted in tuberculosis. Histopathological diagnosis were madein 58 cases as follows: endoscopic biopsy(n=29), operation(n=12), follow up examination(n=14), tissue culture(n=1), laparoscopic biopsy(n=2). There were 30 men and 28 females. The mean age were 36 yrs(range:16-67), 34 patients were in the second and third decade. The distribution of lesions, morphological characteristics, mucosal changes, ulcer shape and depth, associated with other tuberculous lesion, multiplicity were retrospectively analyzed in the findngs of double contrast barim enema. The commonest clinical symptoms were abdominal pain(n=35, 60%) and diarrhea(n=18, 31%). The chest radiography revealed association of pulmonary tuberculosis in 39 cases. Tuberculous lesions of other organs except chest were present in five cases. Double contrast barium enema findings showed a thickening and irregularity of intestinal mucosal fold(n=29, 50%), bowel contraction and/or shortening(n=26, 45%). There were small, nodular, superficial ulcers in the case of ulcer depth less than 2 mm in the depth. As many as 39 cases(67%) in this series had two or more lesions in the intestinal tuberculosis. The involved segment revealed symmetrical feature(n=21, 36%). Inflammatory pseudopolypoid lesions were noted in 30 cases(52%). The ileo-cecal region was involved in 38 cases(66%). The ascending colon(n=25, 43%) was the second commonest site with or without contiguous cecal involvement. Lesions in terminal ileum, ileo-cecal valve, transverse colon, descending colon and appendix were seen in 19, 18, 18, 12 and seven cases, respectively. Among 18 cases of ileo-cecal valve lesions, there were incompetency in nine cases(16%) and wide gapping in four cases(7%). In 14 cases, tuberculous enterocolitis was diagnosed by clinical follow-up and double contrast barium enema study. 12 out of 14 cases, the findings of double contrast barium enema of tuberculous enterocolitis were normalized after anti-tuberculous medication. However, two cases showed more aggravated state than before due to inappropriate treatment. Double contrast barium enema plays an important role in the diagnosis of tuberculous enterocolitis, determines the type, site and extent of the involvement and helps in treatment guide by using sequential studies.

방사선 장해에 대한 백작약의 방호효과

오헌,박혜란,정일윤,김성호,조성기 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.3

방사선 생체 손상에 대한 방호 효과를 나타내는 천연물을 검색하기 위한 일환으로 한의학에서 보혈양혈 탕제에 널리 사용되는 백작약 (peaonia japonica)을 열수총추출물, 에탄올분획, 조다당분획으로 나누어 방사선에 의한 산환적 손상 경감 효과를 검정하였다. 사람 림프구에서 단세포전기영동 (single-cell gel electrophoresis; comet assay)을 수행하여 DNA 손상 경감정도를 관찰하였으며, 마우스에 백작약 추출물을 투여한 다음 8 Gy의 감마선을 조사한 후 간에서 지질과산화 정도를 살펴보았다. 에탄올분획 처리군에서 높은 DNA 손상 경감효과를 확인할 수 있었으며, 지질과산화 억제작용 및 라디칼 소거효과 또한 에탄올분획이 높은 효과를 나타내어 에탄올분획이 방사선 방호에 주된 역할을 하는 것으로 사료된다. 이상의 결과로 보아 백작약은 방사선의 산화적 손상에 대하여 효과적으로 세포 DNA를 방호하고, 생체막의 주성분인 지질의 과산화를 억제하는 것으로 관찰되어 특히, 독성이 거의 없는 천연물이라는 관점에서 방사선 방호제로 적용이 가능할 것으로 사료된다. We investigated the effect of Paeonia japonica (PJ) on radiation-induced oxidative damage to macromolecules in vitro and in vivo. The PJ reduced the tail moment (TM), which was a marker of DNA strand break in single-cell gel electrophoresis (SCGE; comet assay) in the human peripheral blood lymphocytes. Lipid peroxidation in the liver of the ICR mouse, measured as malondiadehyde (MDA), was also reduced by PJ administration. Ethanol fraction of PJ was more effective than polysaccharide fraction of that on reduction of TM in SCGE and lipid peroxidation. Also, Their activities to scavenge DPPH radicals and hydroxyl radicals were observed in vitro, and the activities were due to its ethanol fraction. It is plausible that scavenging of free radicals by PJ extract may have played an important role in providing the protection against the radiation-induced damage. These results indicated that Paeonia japonica might be a useful radioprotector, especially since it is a relatively nontoxic natural product.

자궁선근증의 고찰

조환성,이해혁,정성윤,김성욱,이순곤,남계현,이임순,이권해 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Objective: This study was designed to estimate the frequency and risk factor for edenomyosis Methods: The clinical records of 623 women undergoing hysterectomy were retrieved in the Department of Obstetrics and Gynecology, Soonchunhyang University Hospital, during 5 years, from Jan. 1995 to Dec. 1999. Results: The following result were obtained. 1. Adenomyosis was found in 247 of 623 patients(39.6%) 2. The highest incidence was 40-49 years of age group, an incidence of 46.6% and mean age was 44.7 years. 3. Adenomyosis was more frequently observed in parous women than non-parous women, such as 93.1% and 6.9%, respectively. 4. Dysmenorrhea, metrorrhagia, pelvic pain and vaginal bleeding were common symptom of adenomyosis, an incidence of 28.7%, 19.0%, 18.2% and 16.2%, respectively. 5. Myoma was the most combined disease in adenomyosis, showing the incidence of 54.7%. 6. Grossly, the size of uterus was enlarged more than 10 weeks gestational size in adenomyosis, an incidence of 61.9%. 7. Preoperative diagnostic accuracy of adenomyosis was 6.9%. Conclusion: The results show that deeply understanding of the common symptoms and epidemiology of adenomyosis improve the preoperative diagnostic accuracy.

Quartz 자극에 의한 iNOS 발현의 자가조절

김경아,남혜윤,문제혁,정진숙,임영,정치경 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.4

목적 : Rat2 섬유모세포에서 α-quartz에 의해 유도된 NOS의 조절기전을 알아보기 위하여 본 연구가시도 되었다. 방법 : Rat2 세포에 있어서 α-quartz 자극에 의한 NO와 H₂O₂의 분비 및 iNOS의 발현을 관찰하였고, iNOS 억제제인 L-NIL과 H₂O₂ 억제제인Catalase를 전처리하고 마찬가지로 NO와 H₂O₂분비 그리고 iNOS의 단백발현 정도를 western blot-ting을 이용하여 관찰하였다. 결과 : o-quartz는 Rat2 세포에서 iNOS에 의한NO와 H₂O₂의 분비를 유도하였다. L-NIL은 R3t2세포에서 D-quartz에 의한 H₂O₂의 분비와 iNOS발현을 완전히 억제하였다. Catalase로 전처리 하였을 때 o-quartz로 유도된 iNOS에 의하여 생산증가된 H2Oa가 iNOS를 억제하는 autoinhibitorypathway가 차단되어 결과적으로 H₂O₂의 분비와 iNOS의 발현이 증가하였다. 결론 : Rat2 세포에서 α-quartz에 의하여 유도된iNOS는 H₂O₂의 분비를 촉진하고 이 H₂O₂는 iNOS기능을 조절하는 자가조절기전에 중요한 역할을 하는 것으로 생각된다. Objectives : This study was performed in order to investigate the molecular mechanism regulating nitric oxide synthase (NOS) induced by α-quartz in Rat2 fibroblast. Methods : α-quartz-induced nitric Oxide (NO) anti H₂O₂ formation anti α-quarts-induced iNOS protein expression in Rat2 fibroblast were monitored. With iNOS inhibitor (L-N6- (1-iminoethyl) lysine hydrochloride, L-NIL) or antioxidant (catalase), we observed NO and H₂O₂ formation and iNOS protein expression in Rat2 fibroblast stimulated with α-quartz. Results : α-quartz stimulated iNOS-induced NO and H₂O₂ formation in Rat2 fibroblast. L-NIL inhibited H₂O₂ formation and iNOS protein expression by α-quartz in Rat2 fibroblast. Pretreatment with catalase blocked the autoinhibitory pathway of iNOS by iNOS-induced H₂O₂ therefore H₂O₂ and NO production and iNOS protein expression were increased in Rat2 fibrobalst stimulated with α-quartz Conclusion : α-quartz-induced iNOS Stimulated H₂O₂ formation in Rat2 fibroblast. INOS-induced H₂O₂ by α-quartz plays an important role in the autoinhibition pathway for regulating the iNOS function in Rat2 fibroblast