Bochner 방법을 응용한 Shigella의 약제내성 제거

최정현,이제철,설성용 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.1

To study the optimal curing condition on selective medium described by Bochner et al. and modified medium, six strains of S. flexneri and 2 strains of S. sonnei were tested for minimum inhibitory concentration(MIC) of quinaldic acid and cured resistance pattern. Also, changing patterns of elimination according to incubation temperature were investigated at 37℃ and 42℃. MICs of Shigella on original Bochner medium and modified medium were 50 5o 400㎍/㎖. But MICs of Shigella were above of 400㎍/㎖ on the Bochner's medium excluding tetracycline and sodium phosphate. There were no differences in MIC between at 37℃ and 42℃. On the Bochner's medium, losses of drug resistance were more effective than other medium and cured resistance pattern was partial or total of original resistance, regardless of incubation temperature. Frequencies of curing were low on modified Bochner's medium, but most of cured resistance pattern were total resistance of Shigella. Frequencies of curing at 42℃ were similar to 37℃, but some strains showed resistance loss only at 42℃. MICs of 8 strains of Shigella were above of 400㎍/㎖ and no strains detected resistance loss on the both tryptic soy agar and tryptic soy broth.

한국수출기업의 무역클레임관리에 관한 실증연구

최정호,이제현 서강대학교 경영학연구원 2004 서강경영논총 Vol.15 No.1

International trade claims in Korea have been brought up in the past 10 years in terms of various types as well as the number, which have been gradually increased in proportion to the growth of the trade volume. Since 1991, however, no attempt has been done for the study of claims under the changed international environments.The major purposes of this study are to investigate the relationship between the degree of identification of international trade claims and the characteristics of Korean export firms, and to analyze how effectively Korean export firms manage international trade claims. The major findings of this study are as follows: The more export-oriented Korean export firms are, the higher the degree of identification of international trade claims. The more frequent the international logistics · claims are, the higher the degree of identification of international trade claims. The more frequent the international logistics costs are, the higher the degree of identification of international trade claims. The more frequent the D/A or D/P export transactions are, the higher the degree of identification of international trade claims.

우리 전통의약학 자료의 보호 방안 : WIPO의 전통지식에 관한 지적재산권 보호 논의의 대응 방안 모색 Finding ways against discussions of intellectual property protection on traditional knowledge by WIPO

이제현,김용진,최환수 대전대학교 한의학연구소 2004 한의학연구소 논문집 Vol.12 No.2

WIPO international government committee will finish to develop toolkit for making documentation on discussions of intellectual property protection with TK, GR until the late 2003. As they construct DB of TK, GR, it becomes in a hurry to construct DB of TK information in Korea. We looked into application of copyright in force and a lot of problems in terms of analyzing medical books in the resent condition or protecting them in Korea. There are 29 kinds which have been lost, 12 kinds which could have read in parts, 136 kinds which have been existed of Korean medical books before 1900. There are also a lot of medical books which have been published after 1900 and still are unpublished. It would be 406 medical books from China totally, 111 kinds from Chosun Dynasty version, 134 kinds from Euybangeuchui, 225 kinds from Hyangyackjibsungbang, 78 kinds from Dongeuybogam. We consider to know whether Korean medical books could been applied to the copyright in force or not in accordance with discussions of WIPO on the period, subject(author), contents of protection. If it is applied to the copyright in force, it will be decided to revise according to discussions of WIPO. On the other hand, the problem which is occurred to protect Korean medical books is friction with China as importing their medical books, to find our medical books actively which has been unpublished. Finally, We have to revise administration and intellectual property standards or protect our medical books by making new administration laws.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

뇌하수체 종양 제거술 시행 후 뒤늦게 발생한 횡문근 융해증과 동반된 중추성 열

장제혁,최규남,김일환,노은지,김윤정,유충헌,고정해,박봉수,김태균,권민정,이순희,박정현 인제대학교 2008 仁濟醫學 Vol.29 No.-

Body temperature is controlled by thermoregulatory center of the hypothalamus. We report a case of 24 years old man with central fever. He was subjected to a partial excision of pituitary tumor compressing optic chiasm four years ago. He has received hormonal therapy for panhypothyroidism after removal of pituitary tumor. And He received gamma knife operation for partially contrast-enhancing masses in suprasellar and both hypothalamic areas that is probably postoperatively remnant or recurrent tumor of pituitary adenoma. One year after gamma knife operation, he presented with a febrile syndrome of unknown origin including rhabdomyolysis. All usual investigations proved negative. We diagnosed him as central fever with rhabdomyolysis. He received medical ICU care with cooling bed, ice pack. And his symptom improved. Postoperative hyperthermia may result following resection of the pituitary tumor. When central fever is suspected taking note of past history, a quick recognition of course of fever can help reduce the using of unnecessary antibiotics and hospital stay.

월경전기증후군의 심리적 요인에 관한 연구

이현제,최용성,권택술 大韓神經精神醫學會 1994 신경정신의학 Vol.33 No.2

Authors surveyed how the psychologic factors, especially the personality traits were related to the premenstrual syndrome, using PAF and MMPI, with 110 married nurses and 106 unmarried nursing students. The results were as follows : 1) There were no relations between the premenstrual syndrome and the demographic characteristics. 2) The high-scored types in PAF were turned out to be fatigue, lability, anxiety and atypical depressive features in rank order. On the contrary, the low-scored types in PAF were turned our to be increased well-being, hostility/anger, miscellaneous mood/behavior changes and impulsivity in rank order. 3) In the mean comparison of the MMPI scales among three clusters Pa, Pt and Sc scales showed statistically significant relations both in married and unmarried group, also D scale showed statistically significant relation in married group only and Hs, Ma scales showed statistically significant relation in unmarried group only. 4) In the mean comparison of the MPDS scales among three clusters BDL, ANT, CPS, PAG, PAR and STY scales showed statistically significant relations both in married and unmarried group, also DEP scale showed statistically significant relation in unmarried group only.

성선자극호르몬유리호르몬 길항제인 Antide가 수컷 생쥐의 유해감각예민도에 미치는 영향

박정현,박제민,김명정,이국희,최상헌,장세헌 대한생물치료정신의학회 2001 생물치료정신의학 Vol.7 No.1

To investigate testosterone effect on nociception, tail flick latency(TFL, sec, 50±1℃) was measured before and after administration of antide(a gonadotropin releasing hormone antagonist) in male mice(Institute of Cancer Research, age of 7-8 weeks, weight 30.5-37.5gm). Testosterone replacement effect on TFL was also examined in antide pre-treated male mice. Experiment 1:30 male mice were randomized into 3 groups(N=10 each). Antide 100㎍/kg, 300㎍/kg or same volume(5.7ml/kg) of 0.2% bovine serum albumin was administered intraperitoneally. TFL was measured before and at 3,6, 9, 12 and 24 hours after injection. Experiment 2 : 40 novice male mice were randomized into 2 groups(N=20 each) and both groups were pre-treated with antide 300㎍/kg i.p.6 hours after antide injection, testosterone 1mg/kg or same volume(5.7ml/kg) of sesame oil was administered i.p. TFL was measured before and 6 hours after antide pre-treatment and 30 min after testosterone/vehicle injection. 1) TFL was significantly shortened by antide in dose dependent manner, In control group, baseline TFL(mean±SD) and those measured at 3,6,9,12 and 24 hours after injection were 4.8±0.7, 5.6±0.7, 5.3±0.6, 5.4±0.6 and 4.7±0.6 sec, respectively. In antide 100㎍/kg group, TFLs were 5.2±0.9, 5.1 ±1.1, 4.2±0.9, 4.4±0.8, 5.1±0.8, and 4.7±0.8 sec, respectively. Compared with baseline, significant hyperalgesia appeared 6 and 9 hours after treatment. TFL measured at 6 hours after treatment was significantly shorter than control. In antide 300㎍/kg group, TFLs were 5.6±0.4, 4.5±0.9, 4.3±0.6, 3.9±1.0, 4.6±0.8 and 4.7±0.9 sec. Significant hyperalgesia appeared 3 hours after treatment and continued thereafter. TFLs measured at 3, 6 and 9 hours after treatment were significantly shorter than control, and TFL at 9 hours was significantly shorter than antide 100㎍/kg group. 2) Hyperalgesia induced by antide pre-treatment was recovered by testosterone replacement. In control group. TFL was shortened by antide pre-treartment(baseline : 6.1±1.0 sec ,6 hr after antide : 4.7±1.0 sec) and there was no significant change after vehicle injection(4.6±0.8 sec). In testosterone group, shortening of TFL induced by antide pre-treatment(baseline: 6.2±0.8 sec; 6 hr after antide: 4.6±0.9 sec) disappeared after testosteronen replacement(0.6±0.8sec). From these results, it is suggested that testosterone has a role of maintaining baseline antinociception and acute decrease in testosterone level results in hyperalgesia.

WIPO의 전통지식 등의 지재권 설정 논의에 관한 개략적 방안 모색

이제현,김용진,최환수 대전대학교 한의학연구소 2004 혜화의학회지 Vol.12 No.2

The international government committee is progressing their agreements about intellectual property protection of traditional knowledge(TK1, gene resource(GR), folklore(FL) in WIPO. It is in the course of selection with precedence of TK, GR, FL in WIPO, focused on discussions about listing of TK documents, standardization of DB construction, sharing and profit distribution of GR. There are disagreements between developed countries and developing countries about intellectual property protection agreements of TK, GR. The developed countries insist on using the existing intellectual property protection, but the developing countries ask new ones on character of TK, GR. It causes intangible assets to be valuable trade properties in future world trade. This research Groped for a summary program about intellectual property protection of traditional knowledge(TK) etc. debating in WIPO. This program confirms that such as TK, GR etc. not only to be the cultural property accumulated in human history, but also to be the original resource may be using at present. Therefore, we suggest that the focus of discussion should transfer to UNESCO instead of WIPO which only deal with the intellectual property protection. Besides, the main body which protecting and supporting TK should become its holding organization so as to achieve more effective management about it. In order to protect and support TK, the government should have firstly the recognition that TK is the property belongs to the country. By this viewpoint, it needs to setup DB through overall excavation of the unofficial knowledges in order to protect and support these TK, GR. Because the positive dealing with those WIPO's discussion means to support our TK, GR, so there should have some reorganization about existing related ones, and also needs systemic supporting policies & management system.

중증 재생불량성 빈혈 환자의 동종골수이식 후에 병발한 간정맥폐색질환(Hepatic Veno-Occlusive Disease)의 임상 경과 및 혈액응고 인자들의 변화

이제환,이규형,최성준,민영주,김신,이정신,김상희,박찬정,지현숙,김우건 대한조혈모세포이식학회 1999 대한조혈모세포이식학회지 Vol.4 No.2

연구배경: 재생불량성 빈혈 경우에는 조혈모세포 이식 후 VOD의 발생에 관한 보고가 거의 없는 실정이다. 저자 등은 cyclophosphamide와 antithymocyte globulin으로 전처치 후에 동종골수이식을 시행한 재생불량성 빈혈 환자들을 대상으로 하여 VOD의 발생과 임상 양상, 혈액응고 인자들의 변화에 관하여 분석하여 보았다. 방법: 재생불량성 빈혈로 동종골수이식을 시행 받은 13예 환자를 대상으로 하였다. 임상적 기준에 따라 VOD를 진단하였으며 임상 경과에 따라서 경중을 분류하였다. Protein C, protein S, antithrombin Ⅲ, tPA, PAI-1 등의 혈액응고 인자들을 전처치 투여전, 골수 주입당일, 이식 후 제7, 14, 21일에 측정하였다. 결과: 7예(53.8%)에서 VOD가 발생하였으며, 경증 5예, 중등도 2예였다. VOD군에서 혈소판치가 20.000/㎕ 이상을 유지하는 데까지 걸리는 시간이 더 길었다(p=0.026). 또한 VOD군에서 적혈구 및 혈소판의 수혈 요구량이 각각 유의하게 많았다(p=0.025, 0.015). 혈액응고 인자들의 변화를 보면, 제 0일에 VOD군에서 PAI-1의 혈중 농도가 유의하게 더 높았다.(p=0.034). 결론: 재생불량성 빈혈환자에서도 조혈모세포 이식 후에 VOD의 높은 발생률을 관찰하였으며, 대부분이 경증이었다. VOD 환자에서의 혈액응고 인자들의 변화는 PAL-1이 VOD의 감별 진단을 위하여 이용될 수 있음을 시사하고 있다. Background: There are few reports about the occurrence of hepatic veno-occlusive disease(VOD) after bone marrow transplantation (BMT) for severe aplastic anemia (SAA). We analyzed the incidence and clinical outcomes of VOD and changes of coagulation factors in patients with SAA who were conditioned with cyclophosphamide plus ATG and underwent allogeneic BMT. Methods: Thirteen patients with SAA who underwent allogeneic BMT were included. A diagnosis of VOD was made according to clinical criteria, and severity of VOD was classified according to clinical course. Plasma levels of protein C, protein S, antithrombin Ⅲ, t-PA, and PAI-1 were determined before preparative chemotherapy, in the day of marrow infusion, and days 7, 14, and 21. Results: VOD occurred in 7/13(53.8%). Five were mild VOD and two were moderate VOD. Time to platelet ≥20,000/L was slower (p=0.026) and red cell and platelet transfusion requirements were higher (p=0.025,0.015) in patients with VOD . The mean plasma level of PAI-1 at 0 were higher in patients with VOD (p=0.034). Conclusion: We observed the high incidence of VOD after allogeneic BMT in patients with SAA. A majority of cases of VOD were mild. The changes of PAI-1 after BMT may be useful marker of hepatic VOD

우심방과 우심실의 거대 심장 혈관종 1예

김제열,조상호,김건일,허경림,김현숙,조구영,최영진,이원용,임종윤 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Cardiac tumors, especially the primary tumors involving any part of the heart are extremely rare and its relative incidence has been reported to be approximately 0.02%. We report a patient with huge cardiac hemangioma who complained of shortness of breath, general weakness, and dizziness, Imaging study by echocardiography and computed tomography of thorax revealed a huge lobulating mass like a bunch of grapes in the right chamber of heart. The surgical exploration of thorax was performed and a histological diagnosis of spindle cell hemangioma was obtained by microscopy. The patient was treated by surgical resection of the tumor and doing well after surgery. Our experience indicated that prompt diagnosis and treatment of cardiac hemangioma is imperative for patients' prognosis.