당뇨병 백서의 대동맥에서 열 자극이 혈관 운동성에 미치는 영향

구성모,이순희,한정훈,정기영,김인겸,김정국,하승우,김보완 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.5

연구배경:열자극은 열자극 단백질의 생성을 유도하여 유해한 자극에 대한 세포내 반응을 유도하는 것으로 알려져 있다. 열자극 단백질은 여러 조직에서 발현되며 일부 열자극 단백질은 산화 스트레스에 대해 세포를 보호하는 기능을 나타내기도 한다. 최근 보고에 의하면, 일부 열자극 단백질은 혈관운동성과 관련이 있다고 한다. 당뇨병은 수축물질에 대한 과다한 수축과 이완물질에 대한 이완능의 저하를 특징으로 하는 혈관 운동성의 장애를 초래하는 대표적인 질환이다. 이런 혈관운동성의 장애는 당뇨병성 혈관병증의 병인에 중요한 요소이다. 그러나 고혈당의 조건에서 혈관 운동성에 대한 열자극의 효과에 대해서는 잘 알려져 있지 않으며 그 기전에 대해서도 밝혀진 것이 없다. 이에 스트렙토조토신으로 당뇨병을 유발한 백서 대동맥에서 열자극이 혈과운동성에 미치는 영향에 대하여 연구하였다. 방법:당뇨병 백서 대동맥을 적출하여 대동맥환을 준비하고 장치한 후 42도에서 45분간 열자극을 가한군과 열자극을 가하지 않은 군사이의 혈관 수축물질에 대한 수축반응과 이와물질에 대한 이완반응을 측정하였다. 혈관반응을 측정한 후 대동맥환을 얼리고 열자극단백질 HSP 70의 발현을 조사하였다. 결과:당뇨병 백서와 정상 대조군 백서의 대동맥에서 KCI과 phenyephrine에 대한 혈관 수축반등은 열자극을 준 군에서 주지 않은 군에 비해 현저하게 증가하였다. 열자극은 당뇨병과 정상 대조군의 대동맥에서 혈관수축반응의 증가 뿐만 아니라 HSP 70의 발현을 의미있게 증가시켰다. 정상 대조군의 대동맥에서 아세틸콜린에 대한 이완반응은 열자극을 준 군과 주지 않은 군사이에 통계학적 유의한 차이는 없었다. 반면에 당뇨병 백서의 대동맥에서는 열자극을 준 군이 주지않은 군에 비해 아세틸콜린에 대한 이완반응이 유의하게 감소하였다. 결론:이상의 결과는 당뇨병과 정상쥐에서 열자극에 대한 혈관 수축반응의 증가는 HSP 70의 발현을 유도하여 일어날 수 있음을 시사하여 당뇨병군에서 정상 대조군에 비해 열자극에 대한 이완반응이 감소하는 기전은 많은 연구가 되어야 할 것으로 생각된다. Background: Heat shock has been known to change cellular response to noxious stimuli by inducing heat shock proteins(HSP). HSP are expressed in many tissues, and increased expression of some HSP enhances the survival of cells exposed to oxidative stress. Recently, Some HSP are known to associate with vascular reactivity. Under diabetic conditions, there is a abnormal vascular reactivity to relaxing or contracting factors. Abnormal vascular response to some stimuli is an important role in the development of diabetic complications. However, the effects of heat shock on the vascular reactivity in diabetic condition is unclear. Therefore, we investigated effects of heat shock on the vascular reactivity in isolated aorta of streptozotocin-induced diabetic rats. Methods: After mounced in organ bath, aortic ring preparations were exposed to 42℃ for 45 minutes followed by being subjected to contraction and relaxation in 4 hours. Tissues were frozen for measurement of HSP 70 and phosphorylation of myosin light chain after functional study. Results: Heat shock not only increased expression of HSP70 in rat aorta but also augmented contraction to KCI and phenylephrine in the aorta of control and diabetic rats(p<0.05). Relaxation responses to acetylcholine (Ach) were not changed in the aorta of control rats with and without heat shock for 45 minutes. However, heat shock for 45 minutes decreased relaxative responses to Ach in the aorta of diabetic rats compared to those in the aorta of control rats. Conclusion: This result suggests that heat shock increases vascular contractility in the aorta of diabetic and control rats through the induction of HSP70 while heat shock seems to decrease relaxative response in the aorta of diabetic rats compared to control rats(p<0.05). Whether heat shock impaired relaxative response in the aorta of diabetic rats deserves additional studies (J Kor Diabetes Asso 25:343~353, 2001).

'1998 한국인 우울증 선별의 날' 참가자들의 사회인구통계학적 특성과 우울증 선별검사 결과

한상익,이성필,고효진,박원명,장계호,유태열,전태연,채정호,방승규,김광수,최보문,이정태,백인호 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.5

연구목적 : 1998년 정신건강의 날 기념 한국인 우울증 선별의 날 행사에 자발적으로 참여한 참가자들의 사회인구 통계학적 특성을 분석하고 우울증 선별검사 결과를 평가하기 위하여 본 연구를 시행하였다. 방 법 : 8개 병원에서 얻어진 619명의 자료를 사용하여 선별검사로 밝혀진 우울증의 유병율과 사회인구통계학적 특성 및 응답자의 정신과적 치료를 포함한 과거력 등을 조사하였다. 결 과 : 우울증 척도 점수는 응답자 전체 평균 57.5±13.8로 경도의 우울 증상이 있는 것에 해당되었다. 전체 응답자의 69.5%(430명)가 최소한 경도 이상의 우울 증상을, 43.1%(267명)가 중등도 이상의 우울 증상을, 18.4%(114명)가 고도의 우울 증상을 보였다. 고도의 우울 증상이 있는 응답자의 56.8%와 중등도의 우울 증상이 있는 응답자의 63.9%가 정신과 치료를 받은 적이 전혀 없었다. 나이가 29세 이하인 것과 전임근무 중인 직업상태는 각각 나이가 60세 이상인 것과 직업이 없는 것에 비교하여 우울증으로부터 보호하는 인자임을 시사하였다. 결 론 : '1998 한국인 우울증 선별의 날' 행사를 통하여 많은 우울증 환자들을 선별해낼 수 있었고, 이들의 우울 증상은 몇몇 사회인구통계학적 특성과 유의한 관계가 있음을 확인하였다. 우울증 선별의 날 행사를 통한 우울증에 대한 교육과 우울증 선별검사는 치료받지 못하고 있는 우울증 환자 및 기존의 치료 중인 환자 모두에게 유용한 프로그램임을 알 수 있었다. Objectives : This study was performed to analyze the sociodemographic characteristics of participants in '1998 Korean Depression Screening Day' and to evaluate the results of the screening test. Methods : By using the survey results of 619 volunteers from 8 hospitals, the authors examined the prevalence of depression detected at the screening test and sociodemographic characteristics and the psychiatric treatment history of respondents. The assessment measure was the Zung Self-Rating Depression Scale. Results : The mean depression score of all participants was 57.5±13.8 and it came under mild depression. Of all participants, 69.5%(N=430) had at least mild depressive symptoms, 43.1%(N=267) had at least moderate symptoms, and 18.4%(N=114) had severe symptoms. Never have 56.8% of respondents in the severely depressed range and 63.9% of those in the moderately depressed range had psychiatric treatment. The results suggest that the age group of 29-year-old or younger(relative to 60-year-old or older group) and full-time employment status(relative to unemployment) are protective factors of depression. Conclusions : By '1998 Korean Depression Screening Day', many depressed patients were detected and their depressive symptoms had statistically significant relationships with some sociodemographic characteristics. The results suggest that the education and screening test programs for depressive illness facilitated by Depression Screening Day are useful to the patients regardless of being under current treatment or not.

Drug evaluation based on phosphomimetic PDHA1 reveals the complexity of activity-related cell death in A549 non-small cell lung cancer cells

( Ling Jin ),( Minkyoung Cho ),( Bo-sung Kim ),( Jung Ho Han ),( Sungmi Park ),( In-kyu Lee ),( Dongryeol Ryu ),( Jae Ho Kim ),( Sung-jin Bae ),( Ki-tae Ha ) 생화학분자생물학회 2021 BMB Reports Vol.54 No.11

Cancer cells predominantly generate energy via glycolysis, even in the presence of oxygen, to support abnormal cell proliferation. Suppression of PDHA1 by PDK1 prevents the conversion of cytoplasmic pyruvate into Acetyl-CoA. Several PDK inhibitors have been identified, but their clinical applications have not been successful for unclear reasons. In this study, endogenous PDHA1 in A549 cells was silenced by the CRISPR/Cas9 system, and PDHA1^WT and PDHA1^3SD were transduced. Since PDHA1^3SD cannot be phosphorylated by PDKs, it was used to evaluate the specific activity of PDK inhibitors. This study highlights that PDHA1^WT and PDHA1^3SD A549 cells can be used as a cell-based PDK inhibitor-distinction system to examine the relationship between PDH activity and cell death by established PDK inhibitors. Leelamine, huzhangoside A and otobaphenol induced PDH activity-dependent apoptosis, whereas AZD7545, VER-246608 and DCA effectively enhanced PDHA1 activity but little toxic to cancer cells. Furthermore, the activity of phosphomimetic PDHA1 revealed the complexity of its regulation, which requires further in-depth investigation. [BMB Reports 2021; 54(11): 563-568]

혈액에서 분리된 세균의 항생제감수성 : Vitek System을 이용한 최소억제농도측정 Minimal Inhibitory Concentration Determined by Vitek System

김의종,김선주,신보문,박명희,조한익,김상인 대한화학요법학회 1988 대한화학요법학회지 Vol.6 No.1

Minimal inhibitory concentrations of total 148 strains isolated from blood culture at Seoul National University Hospital were determined by Vitek System. 90% MIC of gram-negative bacteria against amikacin was 2-16㎍/㎖, and those against beta-lactams was > 16㎍/㎖ in general, Except Salmonella spp,. 90% MIC against chloramphenicol and tetracycline was >8㎍/㎖. Range of 90% MIC against vancomycin was ≤.5-4㎍/㎖ in case of garm-positive bacteria, except Enterococcus spp. which showed 90% MIC as >16㎍/㎖. Against ampicillin, 90% MIC of Staphylococcus spp. was >16㎍/㎖, but those of Streptococcus spp. was ≤.25-2㎍/㎖. Streptococcus viridans group showed 90%MIC against penicillin as ≤.03㎍/㎖. Susceptibility of gram-negative bacteria was generally 98% against amikacin, and those of gram-positive bacteria was 80% against vancomycin. 34% of Staphylococcus spp. was resistant of oxacillin. These results may be useful for the primary selection and the dosage determination of antibiotics in the treatment of patients with bacteremia.

Risperidone 조기 중단군 및 장기 유지군의 임상적 특성 비교

김광수,박원명,전태연,배치운,김대진,백인호,이철,김정수,한상익,최보문,장계호,고효진 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.3

연구목적 : Risperidone 투여후 조기 중단한 환자들과 장기적으로 계속 risperidone을 복용하고 있는 환자의 임상적 특성을 평가하고 나아가 risperidone장기 유지와 조기 중단의 요인을 분석하고 정신병리가 약물 투여에 미치는 영향을 조사하고자 하였다. 방 법 : 연구 대상자는 1996년 1월부터 1996년 12월까지 가톨릭대학교 의과대학 부속 8개 병원 정신과에서 risperidone을 투여 시작한 환자 580명중 DSM-IV기준에 의해 정신분열병으로 진단받고 투여 기간이 6개월 미만(조기 중단군)이거나 2년 이상(장기 유지군)을 경과하였으며 의무기록의 누락이 없는 210명을 선정하였다. 두 군간 임상적 특성을 조사하기 위하여 인구학적 변인, 정신병리학적 변인, 약물 투여기록 및 부작용 등을 과거 의무기록을 근거로 평가하였다. 결 과 : 대상환자 210명 중 조기 중단군이 67명(31.9%)이었고 장기 유지군은 143명(68.1%)이었으며 두 군간 인구학적 변인과 정신병리학적 변인에 따른 특징에는 두 군간 차이가 없었다. 두 군간 risperidone의 시작 용량과 변인과 정신병리학적 변인에 따른 특징에는 두 군간 차이가 없었다. 두 군간 risperidone의 시작 용량과 최대 용량은 유의한 차이가 없었으나 유지용량은 조기 중단군에서 4.49(±1.44)mg,장기 유지군에서 3.67(±1.50)mg으로 유의한 차이가 있었다(t=3.698, p<0.05). 또한, 이전 항정신병약물의 사용 기왕력 변인에서 조기 중단군은 기왕력이 없는 환자가 39명(58.2%), 고역가 항정신병약물을 사용한 기왕력이 있는 환자는 27명(40.3%), 저역가 항정신병약물을 사용한 기왕력이 있는 환자는 1명(1.5%)이었고, 장기 유지군은 기왕력이 없는 환자가 58명(40.6%), 고역가 항정신병약물을 사용한 기왕력이 있는 환자는 77명(53.8%), 저역가 항정신병약물을 사용한 기왕력이 있는 환자는 8명(5.6%)으로 유의한 차이가 있었다.(x²=6.559, df-=2, p<0.05). 결 론 : 본 연구결과에 의하면 가급적 환자 상태에 따라 낮은 최적 치료 용량을 투여하는 것이 장기잔의 투여를 바람직한 것으로 생각된다. 본 연구와 같은 대규모의 후향적 자연적 연구는 일반 실제 진료에서 risperidone의 효과 및 기타 다른 측면에서의 유용한 정보를 얻을 것으로 기대된다. Objective : This retrospective naturalistic study was designed to compare the clinical characteristics including psychopathology of two groups of patients, long-term maintenance group and short-term drop-out group, who were taking risperidone. Method : Datas were collected for 210 schizophrenic patients with complete medical records among 580 patients who were enrolled with risperidone administration from January 1996 to December 1996 in 8 affiliated hospital of the Catholic University. The short-term drop-out patients group were assigned to whom treatment period was less than 6 month, and the long-term maintained patients group, treatment period was more than 2 years. We assessed demographics, psychopathology, and other variables related with medication based on past medical records. Results : Among subjects of 210, short-term drop-out patients group were 67(31.9%) and long-term maintained patients group were 143(68.1%). Demographics and psychopathology were not significantly different between two groups. The stating and maximal dosage of risperidone was not significantly different between two groups but the maintenance dosage of risperidone was lower in long-term medicated patients group than short-term drop-out patients group(t=3.698, p<0.05). Additionally, the result of this study showed differences in experiences of past antipsychotic use as following. The number of no previous use of antipsychotic was 39(58.2%), the number of high potency amtipsychotic use was 27(40.3%), and the number of no previous use of antipsychotic was 58(40.6%), the number of high potency ntipsychotic use was 77(53.8%), the number of low potency antipsychotic use was8(5.6%) in long-term maintained group.(x²=6.559, df-=2, p<0.05). Conclusions : According to these results, administration of low therapeutic dosage should be recommended for long-term maintenance as if possible. Multi-center based retrospective naturalistic study like this would be useful for getting informations about efficacy and some other aspects of antipsychotic administration in practial field.

A hybrid PET-MRI: An integrated molecular-genetic imaging system with HRRT-PET and 7.0-T MRI

Cho, Zang-Hee,Son, Young-Don,Kim, Hang-Keun,Kim, Kyoung-Nam,Oh, Se-Hong,Han, Jae-Yong,Hong, In-Ki,Kim, Young-Bo JOHN WILEY & SONS LTD 2007 INTERNATIONAL JOURNAL OF IMAGING SYSTEMS AND TECHN Vol.17 No.4

<P>A PET-MRI fusion system is developed for molecular-genetic imaging. The purpose of the system is to obtain images of the in-vivo human brain using two high-end imaging devices, an advanced PET and an ultrahigh-field MRI. These are the HRRT-PET, a high-resolution research tomograph dedicated to brain imaging on the molecular level, and the 7.0-T MRI, an ultrahigh field version used for morphological imaging. HRRT-PET delivers high-resolution molecular imaging with a resolution down to 2.5 mm FWHM, which is currently the highest spatial resolution available for the observation of the human brain's molecular activities, including enzymes and receptors, which are manipulated genetically, such as reporter genes and probes. The 7.0-T MRI began to reveal submillimeter resolution images of the cortical as well as deep brain areas, down to 250 μm, which allows us to visualize the fine details of the cortical and brainstem areas, including the line of Gennari in the visual cortex and the corticospinal tracts in the pontine area. The current PET-MRI fusion imaging system produces the highest quality images of molecular and genetic activities of the human brain in vivo. It is starting to provide many answers to the key questions about the neurological diseases. Some of these start providing answers to many cognitive neuroscience problems with clear molecular and genetic bases. There is great potential in the PET-MRI for early diagnosis of cancers as well as other neurological diseases, which we were previously unable to diagnose, such as microscopic molecular changes that occur in Parkinson's and Alzheimer's diseases. © 2007 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 17, 252–265, 2007</P>

Quantitative Proteomic Analysis Identifies AHNAK (Neuroblast Differentiation-associated Protein AHNAK) as a Novel Candidate Biomarker for Bladder Urothelial Carcinoma Diagnosis by Liquid-based Cytology

Lee, Hyebin,Kim, Kwangsoo,Woo, Jongmin,Park, Joonho,Kim, Hyeyoon,Lee, Kyung Eun,Kim, Hyeyeon,Kim, Youngsoo,Moon, Kyung Chul,Kim, Ji Young,Park, In Ae,Shim, Bo Bae,Moon, Ji Hye,Han, Dohyun,Ryu, Han Suk American Society for Biochemistry and Molecular Bi 2018 Molecular and Cellular Proteomics Vol.17 No.9

<P>Cytological examination of urine is the most widely used noninvasive pathologic screen for bladder urothelial carcinoma (BLCA); however, inadequate diagnostic accuracy remains a major challenge. We performed mass spectrometry-based proteomic analysis of urine samples of ten patients with BLCA and ten paired patients with benign urothelial lesion (BUL) to identify ancillary proteomic markers for use in liquid-based cytology (LBC). A total of 4,839 proteins were identified and 112 proteins were confirmed as expressed at significantly different levels between the two groups. We also performed an independent proteomic profiling of tumor tissue samples where we identified 7,916 proteins of which 758 were differentially expressed. Cross-platform comparisons of these data with comparative mRNA expression profiles from The Cancer Genome Atlas identified four putative candidate proteins, AHNAK, EPPK1, MYH14 and OLFM4. To determine their immunocytochemical expression levels in LBC, we examined protein expression data from The Human Protein Atlas and in-house FFPE samples. We further investigated the expression of the four candidate proteins in urine cytology samples from two independent validation cohorts. These analyses revealed AHNAK as a unique intracellular protein differing in immunohistochemical expression and subcellular localization between tumor and non-tumor cells. In conclusion, this study identified a new biomarker, AHNAK, applicable to discrimination between BLCA and BUL by LBC. To our knowledge, the present study provides the first identification of a clinical biomarker for LBC based on in-depth proteomics.</P>

적응제어기법에 의한 산업용 로봇의 정밀위치제어

한성현,Le Xuan Thu,김홍래,정인보 한국공작기계학회 2006 한국공작기계학회 추계학술대회논문집 Vol.2006 No.-

In this paper, it is presented a new scheme of adaptive control system to implement real-time control of robot manipulator. Unlike the well-established theory for the adaptive control of linear systems, there exists relatively little general theory for the adaptive control of nonlinear systems. Adaptive control technique is essential for providing a stable and robust performance for application of robot control. The proposed neuro control algorithm is one of learning a model based error back-propagation scheme using Lyapunov stability analysis method. Through simulation, the proposed adaptive control scheme is proved to be a efficient control technique for real-time control of robot system using Digital Signal Processors.

한국형 기분장애 질문지의 타당화 연구

전덕인,윤보현,정한용,하규섭,신영철,박원명 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.5

Objectives : This study aims to test the validity of the Korean version of Mood Disorder Questionnaire (K-MDQ), a Screening instrument for bipolar disorder. Methods : A total of 238 subjects (126 DSM-IV bipolar outpatients and 112 controls without psychiatric history) completed the K-MDQ. Results : The Cronbach's alpha, used to measure the internal consistency of the scale, was high (0.88). Principal component analysis with varimax rotation revealed three factors, which explained 59.5% of the variance. Individual item correlations with the total score were all statistically significant (p<0.001). The mean total score of the K-MDQ was 8.48 in bipolar disorder and 4.51 in non-clinical participants. A total K-MDQ score of 7 or more excluding further two questions was chosen as the optimal cutoff, as it provided good sensitivity (0.75) and specificity (0.69). Conclusion : The results of this study showed adequate validity of the K-MDQ, suggesting that this instrument is useful for screening of bipolar disorder.

단일 공정을 통한 자동차 내장부품(Door trim)의 신뢰성 향상 연구

정선경(Sun Kyoung Jeoung),이평찬(Pyoung-Chan Lee),유승을(Seung Eul Yoo),김보람(Bo Ram Kim),한인수(In Soo Han),조지현(Ji Hyun Cho) 한국자동차공학회 2011 한국자동차공학회 학술대회 및 전시회 Vol.2011 No.11

최근 자동차 산업에서는 경량화, 친환경, 고감성을 중심으로 자동차용 소재를 연구·개발하고 있다. 자동차의 내장재인 Door trim은 실내장식, 차음, 흡음, 추돌시 탑승자 보호의 기능을 갖는 중요한 자동차 내장부품 중 하나이다. 본 연구에서의 Door trim 제조공정인 단일공정 표피 일체화 기술은 동시 압출-사출 기술(ILC; In-Line Compounding)과 표피 일체화 기술(ISF; Intergral Skin Foam)이 복합된 새로운 개념의 기술이다. 이 기술을 통해 기존 3공정을 단일 공정(ILC+ISF)으로 단순화됨으로서 생산성이 향상될 것으로 기대된다. 또한, Door trim의 개발 소재는 다층의 소재를 단일화하여 재활용이 용이하고 친환경 소재 적용을 통해 환경유해물질(VOCs)이 저감되었다. 단일 공정 표피 일체화 기술을 이용한 Door trim의 내구성을 평가하기 위하여 기계적인 물성과 내환경성을 측정하였다. 소재와 부품에서 요구 물성을 만족하였고 내환경성 평가 결과 자동차 내장부품으로 적용 가능함을 확인하였다.