바이러스에 의한 소아 급성 하기도 감염의 유행 및 임상양상(1998년 9월~2002년 8월)

이수진,박은영,오필수,이건희,김광남,이규만,Lee, Su-Jin,Park, Eun-Young,Oh, Phil-Soo,Lee, Kon-Hee,Kim, Kwang-Nam,Lee, Kyu-Man 대한소아감염학회 2003 Pediatric Infection and Vaccine Vol.10 No.1

Purpose : Acute respiratory tract infections are the most common illnesses in children. The great majority of these infections involving lower respiratory tracts infections(LRTI) are caused by respiratory viruses such as respiratory syncytial virus(RSV), parainfluenza virus (PIV), influenza virus(INF), adenovirus(ADV), etc. Our purpose of this study was to examine the seasonal occurrence, clinical and laboratory features of each viral infections. Methods : Nasopharyngeal aspirates(NPA) were collected from 913 hospitalized children on the first day of admission. They were all admitted to Hangang Sacred Heart hospital and diagnosed as LRTIs. The study period was from September 1998 to August 2002. Specimens were inoculated onto HEp-2 cell, LLC-MK2 cell and MDCK cell. Viruses were detected by immunofluorescent method performed at day 10 postinoculation. Respiratory viruses were detected in 251(27%) cases. Medical records of 208 cases diagnosed as acute LRTIs were all taken reviewed and analyzed. Results : The identified pathogens were RSV 122 cases(58%), PIV 30 cases(14%), INFA 20 cases(10%), INFB 21 cases(11%) and ADV 15 cases(7%) during the study period. Outbreaks of RSV infections occurred every year but mostly on December, 1998 and November, 1999. LRTIs by PIV and ADV occurred during all seasons of the year. INFA was isolated moslty on March, 1999 and January, 2000. INFB infections occurred largely on April, 2002. The clinical diagnoses were bronchiolitis 88 cases(43%), pneumonia 87 cases(42%), croup 19 cases(9%) and tracheobronchitis 14 cases(6%). Common symptoms and signs were cough, rhinorrhea, sputum and fever. WBC counts in peripheral blood showed normal findings in 123 cases(59%). Each viral infections also showed some differences in auscultatory findings and chest X-ray findings. Conclusion : We learned that viruses are one of the major and important etiologic agents of acute LRTIs of children in Korea. However, we still need to find out more about its characteristic clinical features and continue studying on their seasonal occurrence to focus on their management and also prevention. 목 적 : 바이러스는 소아 하기도 감염증의 가장 흔한 원인으로 소아에서 흔히 이환되어 모세기관지염, 폐렴, 크룹, 기관 기관지염 등의 하기도 감염을 일으키는 원인 바이러스들의 유행 및 임상 양상을 조사하기 위해 본 연구를 시작하였다. 방 법 : 1998년 9월부터 2002년 8월까지 한림대학교 한강성심병원 소아과에 급성 호흡기 감염증으로 입원했던 환아 중 총 913례에서 비인두 흡인물을 채취하여 바이러스 배양 및 단일 클론 항체를 이용한 면역 형광 검사법으로 251례(27%)에서 호흡기바이러스가 분리되었다. 그 중 모세기관지염, 폐렴, 크룹, 기관 기관지염 등의 급성 하기도 감염증으로 진단 받은 208례의 의무기록을 후향적으로 고찰하였다. 결 과 : 1) RSV가 122례(58%)로 가장 많았고, PIV 30례(14%), INFB 21례(11%), INFA 20례(10%), ADV 15례(7%) 순이었다. 2) 발생시기는 RSV의 경우 1998년 12월과 1999년 11월에 많이 발생하였다. PIV는 연중 비슷하였고, INFA는 1999년 3월과 2000년 1월에 많이 검출되었다. INFB는 2002년 4월에 많이 발생하였고, ADV는 2000년을 제외하고는 연중 비슷하게 검출되었다. 3) 남녀 성별비는 1.8 : 1로 남아가 더 많았고, 중앙연령은 10개월이었다. 4) 임상적 진단에는 세기관지염 88례(43%), 폐렴 87례(42%), 크룹19례(9%), 기관 기관지염 14례(6%)의 순이었고, RSV의 경우 세기관지염이 70례(58%)였다. 5) 임상증상 및 징후에는 각 바이러스 모두 기침(93%), 비루(83%), 가래(74%), 발열(56%)이 많았고, ADV의 경우 발열(89%), 호흡곤란(56%)이 다른 바이러스에 비해서 높았다. 6) 청진 소견으로 천명음이 들렸던 경우는 RSV가 69%, INFA가 45%로 많았고, 건성수포음은 PIV가 52%, INFB가 38%로 많았으며 수포음은 ADV에서 89%로 높았다. 7) 말초 혈액 백혈구 수는 전체 59%에서 정상이었고, 39%에서 증가하였다. 적혈구 침강 속도는 73%에서 증가하였고, C 반응 단백은 21%에서 양성이었다. 8) 방사선 소견은 고찰이 가능하였던 206례 중 각 바이러스 모두 정상 소견이 가장 많았고, 과팽창은 RSV에서 많았다. 폐문 부위 기관지 침윤과 폐경화는 ADV에서 많았고, 하인두 확장은 PIV에서 현저하게 많았으며, 그 외 무기폐 소견도 있었다. 결 론 : 소아에서 급성 하기도 감염증의 중요한 원인을 차지하는 호흡기 바이러스는 각각 발생시기 및 유행양상이 다르고 임상 양상에서도 차이를 보였으나, 이에 더욱 많은 보고와 연구가 진행되어야 할 것으로 본다.

NMR-based Metabolomic Responses of Zebrafish (Danio Rerio) by Fipronil Exposure

이수진,오상아,김성혜,Won-Ho Lee,Juyoung Choi,Hani Lee,Yujin Lee,김석만 한국자기공명학회 2020 Journal of the Korean Magnetic Resonance Society Vol.24 No.4

Fipronil, the phenylpyrazole insecticide, is effective and used in various fields. Especially, fipronil was reliable because it was known to be specific on invertebrate animals than vertebrate animals including mammals. However, fipronil had potential risks that affect vertebrate animals as it blocks the gamma-aminobutyric acid (GABA) receptors that also exists in vertebrates as well as invertebrates. Therefore, it was necessary that harmful effects of fipronil on vertebrates are clarified. For this purpose, the zebrafish (Danio rerio) were used on behalf of vertebrate animals in present study. The zebrafish were exposed to 5 µg/L, 25 µg/L, and 50 µg/L of fipronil during 12, 24 and 72 hours. To closely observe toxic process, 12 hours and 24 hours of additional time point were set in the exposure test. Nuclear magnetic resonance (NMR)-based metabolomics is an approach to detect metabolic changes in organism resulted from external stimuli. In this study, NMR-based metabolomics showed the metabolic changes in zebrafish caused by fipronil exposure. Metabolic analysis revealed that fipronil interfered with energy metabolism and decreased the antioxidant ability in zebrafish. Antioxidant ability decline was remarkable at high exposure concentration. In addition, metabolic analysis results over time suggested that reactions for alleviating the excessive nerve excitation occurred in zebrafish after fipronil exposure. Through this study, it was elucidated that the adverse effects of fipronil on vertebrate animals are evident. The risk of fipronil on vertebrates can be no longer ignored. Moreover, this study has a meaning of practically necessary research for organism by examining the effects of fipronil at low concentrations existed in real environment.

특허정보를 활용한 CCS(CO₂ Capture and Storage) 기술동향 분석

이수진,이윤석,이정구,홍순직,이중범,Lee, Su-Jin,Lee, Yun-Seock,Lee, Jeong-Gu,Hong, Soon-Jik,Lee, Joong-Beom 한국분말야금학회 2015 한국분말재료학회지 (KPMI) Vol.22 No.4

As recognized by all scientific and industrial groups, carbon dioxide($CO_2$) capture and storage(CCS) could play an important role in reducing greenhouse gas emissions. Especially carbon capture technology by dry sorbent is considered as a most energy-efficient method among the existing CCS technologies. Patent analysis has been considered to be a necessary step for identifying technological trend and planning technology strategies. This paper is aimed at identifying evolving technology trend and key indicators of dry sorbent from the objective information of patents. And technology map of key patents is also presented. In this study the patents applied in korea, japan, china, canada, US, EU from 1993 to 2013 are analyzed. The result of patent analysis could be used for R&D and policy making of domestic CCS industry.

비전통 치밀 가스 학술정보 분석

이수진,길상철,김영인,오민수,Lee, Su-Jin,Kil, Sang-Cheol,Kim, Young-In,Oh, Mihn-Soo 대한자원환경지질학회 2014 자원환경지질 Vol.47 No.5

세계 전체 천연가스 소비량은 2010년 113Tcf에서 2040년 185Tcf로 예상되는 가장 빠르게 증가하는 화석연료이다. 전통 천연가스는 비교적 쉽게 개발되는 반면, 비전통 가스는 개발이 보다 더 어렵고 높은 생산비를 필요로 한다. 비전통 가스는 심부가스, 셰일 가스, 석탄층 메탄가스(CBM), 지중 압력대, 가스 하이드레이트 및 치밀 가스 등 6개의 주요 형태로 구성된다. 치밀 가스는 매우 치밀한 지층을 이루는 불투수성의 견고한 암석 중에 부존하는 천연가스로, 사암 혹은 석회암층에 포집된 천연가스이다. 이 연구에서는 치밀 가스 관련 375개 논문을 연도별 발표현황(2000-2014), 국가별 및 연구기관별 논문 수, 국제공동연구 등을 분석하여, 국내 관련 산업 및 기술정책 수립과 연구개발에 유용한 학술자료로 활용될 수 있도록 하였다. Natural gas is the world's fastest-growing fossil fuel, with consumption increasing from 113 trillion cubic feet(Tcf) in 2010 to 185Tcf in 2040. While conventional natural gas streams from the earth relatively easily, unconventional gas finds are more difficult to develop and more costly to produce. Right now, there are six main types of unconventional gas, including deep gas, gas-containing shale, coalbed methane(CBM), geopressurized zones, Arctic and subsea hydrates, and tight gas. Tight gas refers to natural gas reservoirs locked in extraordinarily impermeable, hard rocks(sandstone, siltstone or carbonate sedimentary rocks). In this study, we analyzed total 375 papers(2000-2014) of tight gas by country, institution, international cooperation etc.

천식유발 마우스에서의 폐 내 세포조성 변화와 IL-4 및 IL-10의 발현 양상

이수진,박세종,리천주,장양호,최농훈,Lee, Soo-Jin,Park, Se-Jong,Li, Tian-Zhu,Jang, Yang-Ho,Choe, Nong-Hoon 한국생명과학회 2006 생명과학회지 Vol.16 No.5

본 연구진은 일차로 BALB/C 마우스를 이용하여 항원으로 ovalbumin을 사용하여 천식을 유발하여 폐 내 세포구성에 미치는 영향을 다른 실험방법에 의해 만들어진 천식모델과 비교하여 살펴보았고 이차로 천식 발생에 중요한 역할을 하는 것으로 알려진 IL-4와 IL-10 knock out (KO) 마우스를 이용하여 천식모델을 구축하여 천식인자를 가진 개체에서의 반응과 천식관련인자가 결핍된 개체에서의 반응성의 차이를 살펴보았다. 천식의 유발은 실험 1일째 $20\;{\mu}g$ ovalbumin으로 감작시킨 후 실험 14일에 재감작을 시켰다. 그 후 nebulizer를 이용하여 nasal inhalation을 28일, 29일, 30일에 실시하여 천식을 유발시켰다. 천식 유발의 확인은 기관지폐포 세척술로 채취한 폐 내 세포액을 이용하여 총 세포수 및 염증세포의 증가와 폐 내 세포와 폐조직의 염색을 통해 분포율을 확인하였다. 천식의 발병 과정에서 IgE 관련 과민반응을 주도하는 IL-4와 Th2 세포의 기능을 억제한다고 알려진 IL-10의 면역화학염색을 통해 그 발현정도를 관찰하였다. BALB/c 마우스의 천식유발군의 경우 천식의 특징인 염증세포의 증가와 호산구의 증가와 세기관지 주위 염증세포의 침윤 및 기도 상피의 비후를 관찰할 수 있었다. 따라서 본 실험에 응용된 천식유발 방법은 적합하였으며 천식유발 절차가 성공적으로 수행되었음을 확인하였다. IL-4 및 IL-10 KO 마우스를 이용한 천식유발군의 경우 BALB/c 마우스를 이용한 천식유발군보다 호산구의 증가 정도가 미약하게 관찰되었고, 폐조직 내 염증세포의 침윤 정도도 감소하였다. 천식매개인자인 IL-4와 IL-10의 면역조직화학염색 결과에서도 양성반응이 거의 나타나지 않았다. 본 연구 결과, IL-10이 IL-4와 같이 천식과 관련하여 세기관지 염증 반응을 증가시키는 역할을 하는 것으로 생각된다. Asthma is a chronic inflammatory disorder of the airways, which characterized by bronchial hyperresponsiveness, reversible airflow limitation and respiratory symptoms. Internationally, the prevalence of asthma has been increased over last 3 decades. Recently, several studies of asthma have been reported with gradually increasing importance. To tesify the hypothesis that interleukin (IL)-4 and IL-10 may be an important determinant of the severity of airway inflammation, their expression was studied in mouse model of asthma. BALB/c mouse, IL-4 Knockout (KO) mouse and IL-10 KO mouse were sensitized with intraperitoneal injection of ovalbumin adsorbed to aluminum potassium sulfate, followed by challenges with intranasal ovalbumin on 3 consecutive days. The severity of pulmonary inflammation was assessed by eosinophilia in BAL fluid, number of total BAL cells, histopathological changes in lung tissues, and immunohistochemical staining against IL-4 and IL-10. In BAL fluid, the number of total cells was significantly increased in asthma induced mouse compare to the control. In asthma induced mouse, eosinophil was increased to 56% and neutrophil was 0.2%. In H &E stains, eosinophilic infiltration and epithelium hyperplasia were clearly noticed in asthma induced mouse. In immunohistochemical staining for IL-4 and IL-10, there was no positive reaction in control group. However, very strong reactions were appeared in asthma induced group. In this research, IL-4 and IL-10, which seem to play a central role in allergic asthma, KO mouse was utilized to test the causative relationship between airway inflammation and role of specific cytokine. Asthma induced IL-4 and IL-10 KO mice showed much decreased inflammatory reactions in the number of total BAL cells, in eosinophilic infiltration, and in immunohistochemical stains against diverse inflammatory proteins. These results suggest that IL-4 and IL-10 increase the asthmatic reactions in vivo mice model.

Optimal Condition of Isolation from an Adipose Tissue-Derived Stromal Vascular Fraction for the Development of Automated Systems

이수진,Chae Rim Lee,김기주,류연희,Eunjin Kim,Yu Na Han,문석호,이종원 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.2

Background: The stromal vascular fraction (SVF) isolated from adipose tissue, which contains stem cells as well as other cell types, has been applied in various research fields. Although different enzymatic concentrations and treatment durations have been applied to isolate the SVF, optimal conditions have not been established. Thus, we aimed to establish the optimal conditions for isolation of the SVF from adipose tissue by automated systems. Methods: The SVF was collected from removed adipose tissues of five donors during surgery. The SVF was treated with 0.1% or 0.2% collagenase type I for 20, 40, or 60 min. Then, colony forming unit (CFU) assays and flow cytometry were performed to characterize the adipose stem cells (ASCs). A cytokine array was used to investigate the correlation between colony-formation ability and the secretion of isolated ASCs. Results: Treatment with 0.1% collagenase type I for 60 min resulted in a higher SVF yield, whereas treatment with 0.1% collagenase for 40 min resulted in higher CFU values. In addition, expression of interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1 in the SVF was higher in the high-CFU group than in the low-CFU group. Conclusion: The optimal conditions for isolation of the SVF from adipose tissue were treatment with 0.1% collagenase type I for 40 min. We identified the conditions required for efficient SVF isolation based on high CFU values, and our results will facilitate the development of automated systems.

구지뽕 나무의 엽록체 TrnL-F 영역 염기서열 분석을 통한 특이적 SNP 분자마커의 확인

이수진,신용욱,김윤희,이신우,Lee, Soo Jin,Shin, Yong-Wook,Kim, Yun-Hee,Lee, Shin-Woo 한국식물생명공학회 2017 식물생명공학회지 Vol.44 No.2

구지뽕 나무(Cudrania tricuspidata Bureau)는 일반적으로 이용되는 대표적인 다년생의 약용식물이다. 최근 국제적 추세에 따라 자국의 유전자원의 발굴, 보존 등이 강화됨에 따라 인접국가와 국내 자생 꾸지뽕 계통을 판별 할 수 있는 기준설정에 관한 연구의 필요성이 대두되고 있지만, 분자생물학적 판별 기술의 개발은 아직 미흡한 실정이다. 본 연구에서는 국내 토종과 중국 계통의 구지뽕 나무의 기원을 판별하기 위해 엽록체에 존재하는 trnL-trnF 유전자단편에서 SNP를 이용한 판별 프라이머를 확보하였으며 이를 보완하여 보다 신속하게 판별하기 위하여 ARMS-PCR기술을 이용한 판별마커와 그 조건을 확립하였다. 그러므로, 본 연구에서 개발된 SNP 마커는 다양한 지역에서 서식하는 구지뽕 계통들의 신속한 확인을 위해 매우 유용하게 이용될 것으로 생각된다. Cudrania tricuspidata Bureau is a widely used medicinal perennial woody plant. For conservation and germplasm utilization of the plant, it is imperative to obtaining information regarding the genetic diversity of the plant populations. Although C. tricuspidata is an important medicinal plant registered in South Korea, no molecular markers are currently available to distinguish Korean-specific ecotypes from other ecotypes of different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from chloroplast genomic sequences to identify distinct Korean-specific ecotypes of C. tricuspidata via the amplification refractory mutation system (ARMS)-PCR analyses. Molecular authentication of twelve C. tricuspidata ecotypes from different regions was performed, using DNA sequences in the trnL-F chloroplast intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific C. tricuspidata ecotypes from different regions.

품질기능전개(QFD)를 이용한 중앙행정기관의 기록관 서비스 품질개선에 관한 연구

이수진,이숙희,손은정,오효정,Lee, Su Jin,Lee, Sook Hee,Son, Eun Jeong,Oh, Hyo-Jung 한국기록학회 2018 기록학연구 Vol.0 No.58

공공기관의 기록관은 업무 수행과정에서 생산되는 가치 있는 기록물을 수집·보존하고 동시에 이용자에게 적극적으로 서비스해야 할 책임이 있 는 기관이다. 본 연구에서는 품질기능전개(QFD) 기법을 활용하여 기록관 이 설치된 공공기관을 대상으로 기록관 서비스의 품질을 측정하고 이를 개선하기 위한 우선순위를 도출하고자 한다. QFD의 구성요소를 규명하기 위하여 중앙행정기관 6곳의 기록관 내부이용자를 대상으로 설문조사를 진 행하였고, 문헌연구와 기록연구사와의 면담을 수행하였다. 이후 도출된 이 용자 요구사항의 중요도와 기술특성을 기반으로 품질의 집(HoQ)을 구축 하여 기록관 서비스 기술특성의 중요도와 우선순위를 도출하였다. 연구 결과, 중앙행정기관의 기록관 서비스는 내부이용자의 기록 이용 가능성을 높이는 서비스에 중점을 두어야 하고, 특히 기록물의 검색, 열람, 대출서비 스 지원을 강화해야 함을 확인할 수 있었다. Archives of Government-Affiliated Organizations have responsibility for collecting and preserving valuable records produced during the business processes of their work and for actively serving users at the same time. This study applied Quality Function Deployment(QFD) techniques to analyzing user’s actual requirements and proposing improvements for the archives service quality of central government agencies. In order to derive the components of the QFD, a survey was conducted among internal users of records centers in six central government agencies, as well as a literature study and interviews with working-level records researcher were also conducted. Afterwards, The House of Quality(HoQ) was constructed based on the user’s requirements and technical characteristics derived, and the importance and priority of the recorder service technical characteristics were derived based on it. Based on the results, archives service of central government agencies should focus on improving the usability of records for internal user. Especially, it was confirm that the support for searching, reading and borrowing services should be strengthened.

초등학생에 있어서의 사상성격검사 타당화 연구

이수진,최미화,채한,LEE, Soo Jin,CHOI, Mi Hwa,CHAE, Han 대한한방소아과학회 2017 대한한방소아과학회지 Vol.31 No.1

Objectives The purpose of this study was to validate the biopsychological structure of Sasang Personality Questionnaire (SPQ) measuring Yin-Yang temperament with Junior version of Temperament and Character Inventory (JTCI) for temperament and Body Mass Index (BMI) and Pondera Index (PI) for physical constitution in elementary school students. Methods 903 Korean elementary school students (430 boys and 473 girls) completed SPQ, JTCI, and height and weight measures. Then they were analyzed by their genders and age groups. The correlation between SPQ and JTCI subscales were examined. The differences found in the SPQ and JTCI subscales, BMI and PI among high (30%), middle (40%), and low (30%) SPQ groups were investigated by analysis of variance. The profile analysis was also performed to compare JTCI subscale profiles of three SPQ total score groups. Results The SPQ score was significantly (p<0.001), and positively correlated with the JTCI Novelty-Seeking and negatively with the JTCI Harm-Avoidance. The JTCI Novelty-Seeking score of high SPQ group was significantly (p<0.001) higher than that of low SPQ group, and the JTCI Harm-Avoidance score of low SPQ group was significantly higher than that of high SPQ group. The JTCI subscale profiles for three SPQ groups were significantly (p<0.001) different for boys and girls. Significant correlations between BMI and SPQ were not found. PI and BMI of high and low SPQ groups were not significantly different. Conclusions The SPQ was shown to have robust biopsychological structure in elementary school students. It would be useful clinical measures for Sasang typology in pediatric patients.

부모 동거 미혼자의 계층 의식 및 생활 만족도를 결정하는 요인: 부모 동거 미혼자의 성별에 따른 부모의 사회 경제적 자원의 효과를 중심으로

이수진,Lee, Sujin 대한가정학회 2021 Human Ecology Research(HER) Vol.59 No.4

The purpose of this study is to clarify the influence of parents' socio-economic resources on the class consciousness and life satisfaction of unmarried adult children living with their parents. Data from the 2^nd year (1999) and 22^nd year (2019) of the Korean Labor & Income Panel Study (KLIPS) were used. The study included individuals aged under 25-44 who were unmarried, lived with their parents, and had graduated out of final school. From the 2^nd year (1999), data for 491 people were extracted, and from the 22^nd year (2019), data for 978 people were extracted. The information on the fathers and mothers was obtained from the information on the households data, it was responded by the unmarried children. The main results are as follows. First, one of the characteristics of unmarried children living with their parents is that the average age was higher in 2019 than in 1999. Additionally, although they work and have their own income, they live in houses owned by their parents. Their levels of satisfaction with both the living environment and family relationships were high. It was found that the influence of parents' income increased in 2019 compared with 1999 in the class consciousness. As a factor that influences life satisfaction, the employment status of the father has a positive effect on the life satisfaction for men, whereas the employment status of the mother has a positive effect on the life satisfaction for women.