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구윤혜,김종희,전미언 효성여자대학교 가정대학 학도호국단 1985 家政大論集 Vol.4 No.-
어류의 기질을 조사하기 위하여 어류(고등어, 갈치, 동태)의 총지질함량, 지질분포, 지방산 조성을 측정하였다. 그 결과 총 지질함량은 100g당 고등어는 17.61g, 갈치는 10.09g, 동태는 2.40g이었고, TLC법에 의한 지질의 분포는 고등어, 갈치, 동태 모두 97∼99%가 TG였고, cholesterol, diglyceride, monoglyceride도 소량 있었다. 그리고 지방산 조성은 대부분 C_14∼22 fatty acid였다. 그중 고등어와 갈치는 C_22:6 fatty acid가 가장 높게 나타났다. To investigate the nutrional value of fishes contents of total lipid, composition of"fatty acids, distributions of lipids in fishes (Mackerel, Herring, Hair tail) were-examined. The results were summarized as follow. The contents of total lipid in Mackerel, Hair tail, Alaskan pollack, frozen were17.61%, 10.09%, 2.40% respectively Lipids in all them were about 97~99% oftriglyceride ana cholesterol, diglyceride, monoglyceride were existed only the Mackerel,Hair tail and Alaskan pollack, frozen. Fatty acids were compose4 of that of carbonnumber 14-22; C_22:1 were about 25% in Mackerel Hair tail. C_22:1 were about 25% inHerring, C_22:1 were about 31% in Alaskan pollack, frozen.
대장균의 GlnE 유전자 조절 부위의 염기서열 분석에 관한 연구
박종환,이영규,노연희,구자현 건국대학교 1996 學術誌 Vol.40 No.2
The enzymatic activity of glutamine synthetase (GS) which is a major enzyme assimilating nitrogen in enteric bacteria is modulated by a bicyclic cascade that consists in adenylylation / deadenylylation of GS and uridylylation / deuridylylation of a regulatory protein PII. The adenylylation of GS converts the enzyme to an inactive form. The cunverter enzyme for the adenylylation / deadenylylation cycle is adenylyltransferase (AT), a bifunctional enzyme. It is known that many of regulatory proteins of GS are affected by the nitrogen content in medium. In this study, glnE gene sequence from BamHI to Sali restriction site was determined to elucidate the possibility of its nitrogen sensing ability by searching the consensus sequence(WGCA) found in other nitrogen utilizing operons. The results are as follows ; 1) The nucleotide length of a part of glnE gene was 1203 dp and the initial codon ATG was determined by comparing the sequence with 13 amino acid residues from N-terminus of AT. 2) The probable consensus sequence TTGCA showing high homology with those of other nitrogen utilizing operons was found at 43 bp upstream region from the start point. Actual nitrogen sensing capability of glnE gene should be tested to find the complex regulatory mechanism of GS cascade system for further study.
관상동맥 질환에서 CD14 유전자형에 따른 세균 및 Heat Shock Protein에 대한 반응의 차이
한주용,최수연,조현주,김화평,강현재,구본권,김남중,김효수,손대원,오병희,박영배,최윤식 대한감염학회 2007 감염과 화학요법 Vol.39 No.1
Background : CD14 is the receptor for lipopolysaccharides and heat shock protein (HSP), which has been suggested being associated with increased risk of coronary artery disease (CAD). We investigated whether the response to infectious agents or HSP is different according to CD14 polymorphism in Koreans. Materials and Methods : Antibody titers to Helicobacter pylori, Chlamydia pneumoniae, and human HSP60 (hHSP60) were measured in 48 patients with stable CAD and in 41 healthy controls by ELISA. CD14 genotype was determined by PCR and high-sensitivity C-reactive protein (hs-CRP) was measured. Results : Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly associated with the presence of CAD. CD14 genotype distribution was 31 TT (35%), 43 CT (48%), and 15 CC (17%). To compare the response to the infectious organism and hHSP60, we divided study population into 3 groups; CAD patients with non-TT genotype (group I, n=30), CAD patients with TT genotype (group II, n=18), and normal controls (group III, n=41). Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly different among 3 groups. Though hs-CRP level was significantly different among 3 groups, post-Hoc analysis showed that hs-CRP level was not significantly different between group I and group II (group I: 1.6[1.1-3.5] mg/L and group II: 0.35[0.1-2.0] mg/L). Conclusions : This study suggests that the inflammatory responses to infectious organisms and HSP do not differ according to the CD14 genotype in Koreans. 목적 : 만성적인 세균 감염이나 자가 면역 반응이 동맥경화와 연관되어 있다는 주장이 제기되어 왔다. CD14은 lipopolysaccharides (LPS)와 heat shock protein(HSP)의 수용체로 C(-260)→T 다형성이 관상동맥 질환의 위험과 관련되어 있다고 제안되어 왔다. 본 연구에서는 한국인에서 CD14 다형성에 따른 LPS와 HSP에 대한 반응성을 염증표지자를 측정하여 살펴보고자 하였다. 재료 및 방법 : 안정형 관상동맥 환자 48명과 정상 대조군 41명을 대상으로 Chlamydia pneumoniae, Helicobacter pylori 항체 및 인체 HSP60 (hHSP60) 항체 역가를 enzyme-linked immunosorbent assay (ELISA) 방법으로 측정하였다. CD14 유전자형은 중합효소연쇄반응을 이용하여 결정하였고 hs-CRP를 측정하였다. 결과 : C. pneumonias 및 H. pylori 항체 양성 여부 및 항체 역가, 그리고 hHSP60에 대한 항체 역가는 안정형 관상동맥 질환의 유무와 유의한 상관관계는 없었다. CD14 유전자형의 분포는 TT 30명(39%), CT 31명(40%), 그리고 CC 16명(21%)이었다. 관상동맥 질환 군에서 TT 유전자형은 38%, 대조군에서는 32%로 TT 유전자형과 관상동맥 질환의 유무와는 유의한 상관관계가 발견되지 않았다(P>0.05). CD14 유전자형에 따라 C. pneumonias와 H.pylori 감염 및 hHSP60에 따른 반응에 차이가 있는지 여 부를 알아보기 위해, 관상동맥 질환 군을 다시 TT 유전자형 군과 non-TT 유전자형 군으로 나누어, 전체적으로 세군에서 항체 양성률과 역가를 비교하였다(I군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, II군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, III군: 정상 대조군). 세 군에서 C. pneumonias와 H. pylori 항체 양성률 및 hHSP60 항체 역가에는 유의한 차이가 없었다. 세 군사이에 hs-CRP 값에 유의한 차이가 있었지만, post-Hoc분석에서 II군의 hs-CRP 값이 I군에 비해 유의하게 높지는 않았다(0.35 [0.1-2.0] mg/L in group II vs. 1.6 [1.1-3.5]mg/L in group I, P>0.05). 결론 : 본 연구는 한국인에서 CD14 유전자형에 따라 LPS나 HSP에 대한 반응이 유의하게 다르지 않음을 시사한다.
( Yun Hee Koo ),( Yong Jin Na ),( Mee Young Ahn ),( Han Na Jeon\ ),( Jung In Yeom ),( Kyu Sup Lee ) 대한산부인과학회 2013 Obstetrics & Gynecology Science Vol.56 No.2
Objective This study was performed to compare the expression of CD44 in endometrial stromal cells (ESCs) of women with and without endometriosis and to evaluate the role of CD44 in the adherence of ESCs to peritoneal mesothelial cells (PMCs). Methods A PMC adherence assay was performed to evaluate the adherence of ESCs to PMCs in women with and without endometriosis. The expression of CD44 mRNA was measured by reverse transcription-polymerase chain reaction. CD44 protein was evaluated by Western blot analysis. Results There were no significant differences in the expression of CD44 mRNA and protein in ESCs or in the binding of ESCs to PMCs between patients with endometriosis and controls. Although the expression of CD44 protein was decreased in both women with endometriosis and controls after anti-CD44 antibody treatment, there was no effect on binding of ESCs to PMCs. Treatment of ESCs with peritoneal fluid from endometriosis patients resulted in a significant increase in binding of ESCs to PMCs compared to untreated ESCs in the endometriosis group. Conclusion This study demonstrates that the expression of CD44 protein in ESCs from women with endometriosis might not be directly associated with adherence to PMCs.