균류 분화과정의 유전적 조절기작

박범찬,이환희,박윤희,박희문 충남대학교 생물공학연구소 2003 생물공학연구지 Vol.9 No.1

Fungi are well suited for studying mechanism controlling development and cell differentiation in multicellular eukaryotes. The asexual and sexual reproductive cycles are tightly coordinated during the life cycle of Aspergillus nidulans. The asexual (mitotic) reproductive cycle involves the formation of a number of differentiated cell types. Successful formation of multicellular reproductive structures and spores (conidia) requires the establishment of spatiotemporal gradients in essential transcriptional regulators, such as brlA, abaA and wetA. Altered gene expression is coordinated with critical changes in the movement of nuclei and the regulation of the cell division cycle. Therefore, it has been proposed to define a central regulatory pathway. Several early regulatory genes involved in signal transduction pathway controlling asexual sporulation are also required for activating the central pathway. Sexual (meiotic) reproduction is more complex, requiring the differentiation of several specialized tissue types. Although technical difficulties have limited efforts to study the genetics of sexual fruiting-body formation directly, some recent progress has been made. With the recent availability of mutants blocked in sexual development, understanding the genetic interactions between genes that function primarily in determining sexual sporulation will be achieved in the near future.

프리미돈의 한국인에서의 생체이용율시험

권광일,윤민혁,윤희열,박희찬,권준택,심희옥,김동출 충남대학교 약학대학 의약품개발연구소 2004 藥學論文集 Vol.19 No.-

A reversed phase HPLC method was developed and validated for the determination of primidone in human plasma. Propylthiouracil was used as an internal standard. Calibration curves were linear in the concentration range of 10-120 ng/㎖. The coefficient of variation of the intra- and inter-day precision were below 15%. The coefficient of variation of the accuracy were below 15% in the concentration range investigated. A bioavailability study was performed using the validated HPLC method. Twenty four healthy human male volunteers were orally administered 20 mg of primidone. The pharmacokinetic parameters were calculated using WinNonlin. The mean values of AUC_(LAST) was 85.0±17.7 ㎍ hr/㎖, C_(max) was 4.22±1.04 ㎍/㎖, T_(max) was 1.85±1.13 hr, t_(1/2) was 17.7±2.4 hr. The pharmacokinetic parameters and the HPLC method can be used for the desigm of bioequivalence study of primidone.

Elevated TRAF2/6 expression in Parkinson's disease is caused by the loss of Parkin E3 ligase activity.

Chung, Ji-Yun,Park, Hee Ra,Lee, Su-Jin,Lee, Sun-Hye,Kim, Jin Sik,Jung, Youn-Sang,Hwang, Sang Hyun,Ha, Nam-Chul,Seol, Won-Gi,Lee, Jaewon,Park, Bum-Joon United States and Canadian Academy of Pathology [e 2013 Laboratory investigation Vol.93 No.6

<P>Parkinson's disease (PD) is the second leading neurodegenerative disease, and is known to be induced by environmental factors or genetic mutations. Among the verified genetic mutations of PD, Parkin, isolated from the PARK2 locus, shows an autosomal recessive inheritance pattern and is known to be an E3 ligase. However, the physiological target of Parkin and the molecular mechanism of Parkin-deficiency-induced PD have not been clearly demonstrated until now. It has recently been proposed that inflammation, suggesting as a causal factor for PD, is enhanced by Parkin deficiency. Thus, we examined the relationship between inflammation-related factors and Parkin. Here, we provide the evidence that Parkin suppresses inflammation and cytokine-induced cell death by promoting the proteasomal degradation of TRAF2/6 (TNF-α receptor-associated factor 2/6). Overexpression of Parkin can reduce the half-lives of TRAF2 and TRAF6, whereas si-Parkin can extend them. However, mutant Parkins did not alter the expression of TRAF2/6. Thus, loss of Parkin enhances sensitivity to TNF-α- or IL-1β-induced JNK activation and NF-κB activation. Indeed, si-Parkin-induced apoptosis is suppressed by the knockdown of TRAF6 or TRAF2. We also observed elevated expression levels of TRAF6 and a reduction of IκB in an 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mouse model. Moreover, elevated expression levels or aggregation of TRAF6 were detected in approximately half of the human PD tissues (7/15 cases) and 2 cases, respectively. In addition, TRAF6 and Parkin expression levels show a reverse relationship in human PD tissues. Our results strongly suggest that the reduction of Parkin or overexpression of TRAF2/6 by chronic inflammation would be the reason for occurrence of PD.</P>

커뮤니티공간으로서의 어린이공원 조성에 관한 연구

김연금,김성주,박환지,유다희,최성용,홍승모,Kim, Yun-Geum,Kim, Seong-Joo,Park, Hwan-Ji,Yu, Da-Hee,Choi, Sung-Young,Hong, Seung-Mo 한국조경학회 2008 韓國造景學會誌 Vol.35 No.6

This study deals with the participatory design process of renovating a children's park. The process should proceed with two major elements. Firstly, the children's park should be designed from the perspective of children. Secondly, it should be designed in consideration of the context of the community. This study is divided into two parts. First is the review of theory concerning the meaning and roles of the children's park as a community space, the importance of children's participation in the design and the participatory method of the children. The second is the case study. The site for the case study is Ssial Park in Nowon-Gu, Seoul. The entire process of the case study is with community participation, from identifying the park's problems to the actual construction. At every step, through the participatory programs, the community participates in the decision making. In addition, mutual understanding among participants is pursued through diverse means of communication. The following results of the case study were found. Firstly, diverse participatory programs and the active trial to enable the community to communicate contribute to the sense of ownership and responsibility concerning the park Secondly, the community can negotiate the differences in opinions without needing the help of experts. Of course, there are limits in the case study, such as the fact that the community organization, which is the core of communication and maintenance concerning the park, is not organized through the process of a case study. Another is that more diverse methods, which inform all community members of the participatory process, should have been used.

대장균의 GlnE 유전자 조절 부위의 염기서열 분석에 관한 연구

박종환,이영규,노연희,구자현 건국대학교 1996 學術誌 Vol.40 No.2

The enzymatic activity of glutamine synthetase (GS) which is a major enzyme assimilating nitrogen in enteric bacteria is modulated by a bicyclic cascade that consists in adenylylation / deadenylylation of GS and uridylylation / deuridylylation of a regulatory protein PII. The adenylylation of GS converts the enzyme to an inactive form. The cunverter enzyme for the adenylylation / deadenylylation cycle is adenylyltransferase (AT), a bifunctional enzyme. It is known that many of regulatory proteins of GS are affected by the nitrogen content in medium. In this study, glnE gene sequence from BamHI to Sali restriction site was determined to elucidate the possibility of its nitrogen sensing ability by searching the consensus sequence(WGCA) found in other nitrogen utilizing operons. The results are as follows ; 1) The nucleotide length of a part of glnE gene was 1203 dp and the initial codon ATG was determined by comparing the sequence with 13 amino acid residues from N-terminus of AT. 2) The probable consensus sequence TTGCA showing high homology with those of other nitrogen utilizing operons was found at 43 bp upstream region from the start point. Actual nitrogen sensing capability of glnE gene should be tested to find the complex regulatory mechanism of GS cascade system for further study.

ATD automatic bridge remover를 이용한 새로운 포스트 제거 방법

박윤우,박세희,신혜진,조경모,김진우 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.3

포스트가 축조된 치아를 재근관 치료하는 경우 성공적인 비외과적 재근관치료를 시행하기 위해서는 포스트 제거가 선행되어야 한다. 기존의 포스트를 제거하는 방법으로는 초음파기구, Ruddle post removal system, Eaggler post remover, Masserann kit을 이용하는 방법 등 여러 가지가 소개 되고 있으나 기존의 방법들은 각 기구에 맞게 코어를 삭제해야 하는 시간과 많은 노력이 요구되며 포스트 제거시 치아나 포스트의 파절이 유발되기도 한다 그러나 최근에는 기존의 방법과는 다른 ATD automatic bridge remover를 이용해 포스트를 제거하는 새로운 방법이 소개되고 있으며 코어에 구멍을 형성하는 시간만 소요되어 비교적 빨리 제거 할 수 있다는 장점을 가지고 있다. 따라서 본 증례에서는 ATD automatic bridge remover를 이용해 주조포스트를 제거하고 성공적으로 재근관치료를 시행한 증례에 대해서 보고하고자 한다. It is common for clinicians to encounter endodontically tl·treated teeth that contain posts within their roots. If endodontic treatment is failed, these posts must be removed to facilitate successful nonsurgical retreatment. There have been many techniques such as ultrasonic instrument, Ruddle post removal system, Eggler post remover and Masserann kit developed to facilitate removal of posts from the root canal space. But these methods may be disadvantageous because long length of time required for post removal and fracture of post or teeth. In now days new post removal technique using ATD automatic bridge remover was introduced. Advantages of this method are simple and short time consuming compare to others. This article served as a successful case report of post removal using ATD automatic bridge remover.

초·중·고등학교 학생·학부모·일반교사의 보건교육 영역별 요구도와 보건교육

윤순녕,김영임,최정명,조희순,김영희,박영남,오경순,이분옥,조선녀,조소영,한선희,하영미 韓國學校保健學會 2005 韓國學校保健學會誌 Vol.18 No.1

Purpose: The purpose of this study was to identify the needs of health education in students, their parents and teachers in the elementary, middle and high schools and the current situation of health education class. Method: The subjects of this study were a total of 9450 persons including students, their parents and teachers from 279 schools throughout the country. They were selected through convenient sampling. Data were analyzed through x^(2)­test and ANOVA. Result: Students, their parents and teachers replied that 18 dimensions of health education class (DHEC) are necessary. The four DHEC - healthier life style, sex education, mental health and safety education - showed high educational needs in students, their parents, and teacher. High school students had higher educational need of 'symptom management for daily living' than elementary and middle school students. Students, their parents and teachers in elementary school had higher educational needs of 17 DHEC that those in middle and high school. The percentages of schools with health education class taught by health teachers were 99.2%, 75.5% and 66.0% respectively in elementary, middle and high schools. Health education was given mainly using physical education classes at elementary schools, and creative class hours at middle and high schools. In general, health education took 1-3 hours per week at elementary schools, and less than an hour at middle and high schools. Conclusion: Therefore, based on the results, systematic health education class should begin from elementary school to meet the need of health education in students, their parents and teachers, and further study should be made on the number of hours required and the amount of contents of 18 DHEC.

투사각θ로 던진 투사체의 3차원 Mathematica Simulation

윤희중,박종엽 목원대학교 자연과학연구소 2001 自然科學 硏究論文集 Vol.10 No.2

이 논문에서는 Mathematica 4.1을 사용해서 투사각θ로 던진 투사체의 3차원 운동을 simulation하였다. 포사체 운동은 물리학의 기본이 되는 운동으로 물리학에 대한 기본적인 지식이 있는 사람이면 쉽게 이해할 수 있으나 Mathematica를 이용하여 프로그램을 작성하여 simulation해보면 포사체 운동을 좀 더 확실하게 이해 할 수 있다. 이 논문에서는 Mathematica를 간략하게 소개하고 Mathematica에서 포사체 운동을 simulation하여 대학 물리교육 현장에 적용한 과정을 제시하였다. Three demensional Mathematica simulation of the projectile with evaluation angle θ is presented. With this simulation, students can understand the motion of projectile more easily. We show interactive learning programs as examples to illustrate the concepts of projectile motion. We found that integrating the interactive Mathematica programs into physics class was very effective for improving the teaching and learning of physics in the undergraduate curriculum and for enhancing the ingenuity of students.

水銀, 카드뮴, 크롬이 흰쥐의 肝에 미치는 影響

尹惠禎,朴熙聖 梨花女子大學校 韓國生活科學硏究院 1982 韓國生活科學硏究院 論叢 Vol.30 No.-

肝에 對한 重金屬의 影響을 알아보고자 水銀, 카드뮴, 크롬을 흰쥐의 腹腔內에 單回大量投與와 小量으로 1週, 2週, 3週間 每日 投與하여 肝에 畜積된 重金屬量, 總蛋白質量 變化 및 Thiobarbituric acid value를 測定하여 다음과 같은 結果를 얻었다. 가. 水銀投與群 畜積되는 水銀의 量은 投與量과 期間에 比例하여 增加하지는 않았다. 水銀中毒이 發生하는 時期에는 體重이 減少되었고, 肝의 蛋白質合成도 저해되었다. 水銀은 TBA價(脂肪의 peroxidation)에 영향을 미쳤다. 나. 카드뮴投與群 肝에 카드뮴이 어느 程度까지 畜積되면 더 以上 현저한 薯積作用은 없었다. 카드뮴 中毒 初期에는 蛋白質合成이 促進되었으나 肺에 카드뮴이 어느 程度 畜積되면 蛋白質合成이 저해되었다. 카드뮴은 脂肪의 peroxidation을 多小 저해하였다. 다. 크롬投與群 肝에 蓄積되는 크롬의 量은 投與期間에 따라 增加하였으며 正常群보다는 增加率이 낮았으나 體重이 增加하였다. 크롬中毒初期에는 肝의 蛋白質合成이 促進되었으나 그 以後는 현저히 저해되었다. 크롬은 脂肪의 peroxidation에 크게 영향을 미치지 않았다. Effect of the treated with Hg, Cd and Cr on metal distribution, protein synthesis and lipid peroxidation in the rat liver was studied. The results of this study were as follows: A. In treated with the Hg group: 1. The amount of Hg accmulated in liver was not always proportional to administration amount and time. 2. Initial and last time of Hg toxicity, protein synthesis was accelerated. 3. The body weights were reduced with Hg toxicity occuring. 4. Hg effected on lipid peroxidation. In the initial few days treated with Hg increased lipid peroxidation but after 2 weeks lipid peroxidation were inhibited markdly. B. In treated with the Cd group: 1. The amount of Cd accumulated in liver increased to 78.22±6.18㎍ within first 2 weeks but after more accumulation was not observed. 2. In the early time Cd toxicity protein synthesis was increased. 3. Lipid peroxidation was inhibited more or less by treated with Cd. C. In treated with the Cr group: 1. The amount of Cr accumulated in liver increased with treated time. 2. In the early time of Cd toxicity protein synthesis was accelerated. 3. Cr toxicity did not reduce body weight. 4. Cr did not effect on lipid peroxidation markdly.

간호학생의 중환자실 임상실습 체험 연구

박경숙,황윤영,최은희 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: The purpose of this study is to investigate the perception of nursing students' on-side clinical experience at intensive care unit(ICU), which will help on developing fundamental tool to enhance the effect of their clinical practice. Method: Van Manens hermeneutic-phenomenological method has been used to analyze the data. participants consisted of 74 third-year nursing students who performed the clinical practice at an ICU in C university hospital. The students got the group interviews and contents of the students clinical diaries. Result: Major concerns related to students clinical practice were found as follows : 「being nervous about unfamiliar machines and situations」,「being frustrated about the patients」,「being stupefied from witnessing a death」,「realizing the importance of health」,「realizing individual patient's precious value through family's love」,「realizing the importance of treating a patient as humanbeing」,「realizing the differences between theory and practice」,「modelizing two aspects of nursing: professional and personal」,「readiness to become a capable nurse」. Conclusion: We are able to obtain in-depth understandings about the nursing students lived experience of clinical practice at the ICU. Based upon this, there is a need to develop a better nursing intervention enhance the effectiveness of the nursing students clinical practice.