말초혈액에서 Tg mRNA에 대한 역전사 중합효소 연쇄 반응법의 갑상선 재발암의 분자생물학적 진단

권성일,박기룡,김현영,신채희,임영찬,최영식,박요한,이강대,장희경,이재화,염하용 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.4

연구배경: 갑상선암은 다른 조직에 발생한 암에 비해 비교적 천천히 자라므로 대부분 예후가 양호하지만, 일부에서는 주위 조직으로 침윤하거나 혹은 원격 전이로 인하여 치명적인 결과를 초래할 수 있다. 갑상선전절제술 및 131^I 제거술 후 경과 관찰시 갑상선암의 재발과 전이의 진단에 있어 131^I 스캔과 혈청 Tg의 측정이 현재 임상에서 가장 많이 이용되고 있으나 이 방법에는 여러 가지의 결점이 있다. 최근 Tg mRNA에 대한 RT-PCR법을 이용한 여러 연구결과는 131^I 스캔과 혈청 Tg 측정의 결점을 보완할 수 있는 좋은 보조적인 진단법으로 이용할 수 있을 가능성을 제시하였다. 이에 말초혈액에서 측정한 Tg mRNA에 대한 RT-PCR법이 갑상선 절제술 및 방사성요드 치료 후 갑상선암의 재발 및 전이 유무의 진단에 유용한가를 알아보고자 이 연구 시행하였다. 방법: 분화된 갑상선암으로 진단된 후 갑상선전절제술을 시행받고 방사성요드 치료를 받은 환자 중 현재까지 한차례에 이상 추적 방사성요드 전신 스캔을 시행하고 추적관찰이 가능했던 유두선암 35예, 여포선암 5예를 대상으로 연구를 시행하였다. 대상군은 131^I 스캔 소견상 음성인 군(Group Ⅰ), 잔여조직이 있는 군(Group Ⅱ), 국소전이가 있는 군(Group Ⅲ), 및 원격전이 군(Group Ⅳ)으로 구분하였다. 정상 대조군은 갑상선질환이 없는 10예의 건강인으로 하였다. 대상환자의 말초혈액을 이용한 Tg mRNA에 대해 특이적인 primer를 이용하여 RT-PCR 및 nested RT-PCR을 시행하였다. 결과: 본 연구 결과는 다음과 같다. 1) 131^I 스캔 소견상 음성인 군 21예 중 1예에서 Tg가 양성소견을 보였다. Anti Tg Ab가 양성인 4예 모두 Tg가 음성을 보였다. 잔여조직이 있거나 국소전이 및 원격전이를 보인 군 19예 중 Tg가 양성인 경우는 4예였으나, Tg mRNA는 전예에서 양성이었다. 2) 131^I 스캔에서 국소 및 원격전이 소견을 보인 8예 중 4예에서 Tg가 음성으로 131^I 스캔과 혈청 Tg 사이에 불일치 소견을 보였다. 3) 말초혈액에서 특이적인 primer를 이용하여 RT-PCR 및 nested RT-PCR을 시행한 결과 대상군 40예 및 정상 대조군 10예 모두에서 Tg mRNA가 양성을 보였다. 결론: 본 연구에서 갑상선 절제술 및 방사성요드 치료 후 갑상선암의 재발 및 전이 유무를 평가함에 있어 역전사 중합효소 연쇄 반응법을 이용한 Tg mRNA 측정의 의의는 재평가되어야 한다고 생각된다. Background: Differentiated thyroid cancer is the most common endocrine malignancy. Despite advances in the treatment of thyroid cancer, disease recurrence and metastasis may occur in as many as 20% of patients, and so continues to pose major problems in its clinical management. Serum thyroglobulin (Tg) measurements, by immunoassay, are used to detect residual or recurrent thyroid cancer following thyriod ablation. However, the usefulness of immunoassay is limited by both the requirement for thyroid hormone withdrawal, to attain optimal test sensitivity, and interference by the antithyroglobulin antibody (Anti-Tg Ab). Recent studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of Tg mRNA in the peripheral blood of patients with differentiated thyroid carcinomas. We performed this study to evaluated the usefulness RT-PCR of Tg mRNA in peripheral blood of patients with thyroid carcinoma following a total thyroidectomy and radioiodine ablation therapy. Methods: Forty cases that underwent a total thyroidectomy and radioiodine ablation therapy were included in this study. Of the 40 patients, 35 were papillary carcinomas and 5 were follicular carcinomas. Ten normal control subjects were also studied. Tg mRNA was extracted. Then RT-PCR and nested RT-PCR, were run with specific Tg primers. Concurrently, DNA sequencing of the isolates was carried out to prove the isolates were identical to the nucleotide sequence of the Tg. Results: The Tg was detected in 4 of 19 patients, with either a residual thyroid bed, or metastasis, on a 131^I whole body scan and in 1 of 21 patients with a negative radioiodine scan. Surprisingly, the Tg mRNA was detected in all the patients and normal controls. Conclusion: From our results we can not recommend Tg mRNA, detected by RT-PCR in peripheral blood, as a tumor marker superior to that of the Tg serum level. We consider an intensive re-evaluation of the method is required before considering its clinical applications (J Kor Soc Endocrinol 17:501∼513, 2002).

성인의 급성 심장사에서 정상 심장례의 분석

강신몽,이원태,이한영,서중석,최영식,권일훈,정낙은 大韓法醫學會 2000 대한법의학회지 Vol.24 No.2

The authors analysed 53 cases of sudden cardiac death(SCD) without prominent structural abnormalies of heart out of witnessed 300 cases. Thirty four cases were associated with sleep and 19 with activity or rest. Twenty one cases were classified as having normal hearts, and the rest as structurally mild abnormal ones. Male was predominant, and almost of the victims were suffered in their twenties or thirties. All cases collapsed instantaneously or in very short time with various and not specific terminal symptoms. Inducing or trigger factors are not clarified in almost of the cases. Mechanisms or causes of death could not be defined clearly. We concluded that careful heart examination with detailed clinical history under the appropriate postmortem investigation system is only effective approach for the true mechanism and cause of death.

점증적 최대운동이 항산화 효소의 혈중 농도에 미치는 영향

강신범,이상우,김상권,서해근,김준모 東亞大學校附設스포츠科學硏究所 1999 스포츠科學硏究論文集 Vol.17 No.-

The purpose of this study is to examine how exercise stimulus effects on the boold concentration of antioxidant enzymes. And then, the results of analysing the boold concentration of antioxidant enzymes before and after graded maximal exercise based on high school's 7 males, are as follows: 1. The blood concentration of SOD is found to have a significant increase before and after graded maximal exercise. 2. The blood concentration of CAT is found to have a significant increase before and after graded maximal exercise. 3. The blood concentration of CAT is found to have a significant change. As the above results, the severe exercise of short time is found to increase the blood concentration of SOD as well as CAT. And then, the blood concentration of GPX is found not to have a significant exchange. To examine the mechanism of the above results manifestly, it is considered that the deep study based on exercise style, duration time and exercise intensity, should be continued.

영어 사전의 발전 약사 소고

강신권 The English Linguistic Science Association of Kore 2001 English Linguistic Science Vol.7 No.-

This article is aimed to give a help and guide to the dictionary user by understanding the origin and development of English Dictionary(Monolingual Dictionary). In addition to the general English dictionaries, the ones for the special purposes are taken for examples in order that the users may select and use suitable dictionaries. Moreover, We have made expectation of the aspect of dictionary in the future, especially in an electric information age. As a result, the computerized dictionaries on CD-ROM, Internet, and PC are going to be developed according as the programings of the first-dimensioned dictionary(the paper dictionary) is improved. Therefore, the bilingual-dictionary lexicographer in Korea (English-Korean lexicographer) has to keep in mind the importance of selecting the original fictional), because the features of the original one tend to be those of the bilingual one part In our country, the scholars and experts concerned with this field are to take the bilingual lexicography .and give the help to make a fine dictionary. On the other hand, our government is taking efforts to distribute the computers but has to department to publish the bilingual dictionary. I propose that the Ministry of Education should establish the department of bilingual dictionary(lexicography) suitable to the era of globalization.

영어사전의 편찬원칙과 특성고찰

강신권 한국영어교육연구학회 2003 영어교육연구 Vol.- No.26

This article is aimed to give the advice and help of making better bilingual (English-Korean) dictionary and of getting the dictionary users to understand the monolingual and bilingual dictionaries and to get the merits through the following ways. First, I have described such principles as 1) choosing and recording the entry word, 2) analyzing and arranging the meaning of that entry 3) recording the features of the grammar and phonology. Second, I have shown the way that such general principles are applied to the produce of the bilingual dictionary (our English-Korean dictionary) and also I have not only found the serious problems of making bilingual dictionary in our country but also suggested the ways of solving such problems. Finally, I have compared the paper dictionary with the electronic dictionaries, such as digital diary, software (SW) for personal computer, CD Rom, DB (data based) internet and internet sites which have such many merits as the paper dictionary doesn't. As a result, I have pointed out the importance of making the paper dictionary on the basis of the general principles because such electronic dictionaries mentioned above are made on the basis of making the paper dictionary.

사전 사용에 바람직한 사전 요건

姜信權 한국영어교육연구학회 2002 영어교육연구 Vol.- No.24

This article is aimed to show the requisites of dictionary about the two aspects of morphology and the definition of the meaning. The reason is that a dictionary is indispensable for the English-speaking people, but that there will be a serious misunderstanding in using the words as a non-native speakers, if they are ignorant of the meaning and syntatics so as to avoid such serious mistakes, I point out the requisites of dictionary in order to help them to choose correct dictionary. For this aim, I have compared the monolingual and bilingual dictionaries to find the features of each dictionary. Especially I have investigated the features of morphology and definition of the meaning. As a result, I have showed the differences about the two requisites(e.g. the morphology and the definition of the meaning) between the monolingual and bilingual dictionaries. Moreover, internet dictionary has many merits which the paper dictionary doesn't. And so, I propose again that our Ministry of Education should not only have to supply many computers but also have to teach how to use the computers and have to set up such a department of making bilingual dictionary (lexicography) suitable to the era of globalization.

대량재해시 방사선학적 검사에 의한 개인식별예

권귀향,최득린,강신몽,이혜경,김기정 大韓法醫學會 1992 대한법의학회지 Vol.16 No.1

There cases of mass disaster, KAL accident in Tripoli, express bus accident and park cemetry accident in Korea, were referred to the National Institute of Scientific Investigation, Seoul, Korea for individual identification (ID). All the cases were thoroughly investigated to establish the most scientifically sound ID possible. In addition to evidence from fingerprint, dental, anthropological, visual, pathological, blood typing and superimpose according to the case, X-ray studies were attempted. In KAL case, 52 of 59 were identified by comparison radiography. Differential diagnosis was accomplished in 2 misidentified bodies in express bus case by radiography. Radiologic examination also revealed good characteristics in park cemetry case, however, did not satisfactorily contribute to ID because of restricted information.

실내사무환경의 환경성담배연기(ETS)의 지표물질에 관한 연구

하권철,백남원,박동욱,윤충식,김원,최상준,박지영,최인자,김신범,강태선 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.2

The purpose of this research is to evaluate indoor office environment using the concentrations of nicotine, 3-enthenyl-pyridine(3-EP), and respirable suspended particulate(RSP), which are indicators for environmental tobacco smoke(ETS) and the correlations between indicators and environmental conditions(smoking density, smoking index). The mean air charge per hour (ACH) in smoking rooms was 10.4 and most of the smoking rooms showed non-compliance withe ASHRAE standard value except only one smoking rooms. The concentrations of RSP, 3-EP, nicotine showed log=normal distributions, and became different statistically depending on smoking condition(p〈0.01). The geometric mean concentration of RSP in smoking room was 441.7 ug/㎡ that is far exceeded environmental standard(150 ug/㎡). This implies that fine particulate in smoking room should be carefully controlled considering smoking density and ventilation fate. The mean concentrations of nicotine and 3-EP were 93.4 ug/㎥, respectively. The correlation coefficients between RSP and SI, 3-EP and SI, and Nicotine and SI were 0.67, 0.84 and 0.74, respectively. The correlation coefficient between nicotine and 3-EP, nicotine and RSP, and RSP and 3-EP were 0.76,0.78 and 0.57 respectively.

Pseudomonas sp. LBC505의 섬유소 분해효소 특성

정영철,강신권,김희숙,노종수 진주여자전문대학 1995 論文集 Vol.18 No.-

The Pseudomonas sp. LBC505 isolated from soil, produced cellulolytic enzymes such as carboxymethyl cellulase, avicelase, β-1,4-glucosidase, and xylanase in medium. The cellulase complex exhibited maximal activity at pH6.0-pH6.5, and 50-55℃, and was stable in the range of pH5.0 to 9.0 and up to 65℃ for 30min. The cellulase complex and xylanase activities were completely inhibited in the presense of 1mM HgCl₂ and 1mM AgCl₂. The enzyme activity was stimulated in the presense of 100mM mercaptoethanol, suggesting that sulfhydryl groups play an important role in the catalytic action. The crude cellulolytic enzyme hydrolyze CMC to produced glucose, maltose, maltotriose, maltotetraose, maltopentose and maltohexose by synergistic action of cellulase complex.

주의력 결핍/과잉 운동 장애에서의 정량화 뇌파 소견

김붕년,권준수,신민섭,조수철,홍강의 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.1

연구목적 : 주의력 결핍/과잉 운동 장애(ADHD) 환아군과 나이 및 성별을 일치시킨 정상 대조군간의 정량화 뇌파 소견상에서의 차이를 규명하여 ADHD의 병태생리를 시경생리학적인 견지에서 이해하고자 본 연구를 시행하였다. 방 법 : 스펙트럼 뇌파 분석법을 이용하여, 20명의 약물 비노출 ADHD 환아군과 20명의 정상 대조군간에 차이를 보았다. 분석영역은 8개의 양극성 영역으로, 알파, 베타, 델타, 쎄타등 4개의 주파수 영역에 대해 각각 상대력(relative power), 반구간 비대칭성(interhemispheric asymmetry), 반구간 동시성(interhemispheric coherence)의 값을 구하여, student t-test로 비교 분석하였다. 결 과 : 1) 상대력에 있어서, 환아군이 대조군에 비해 베타파는 두정- 후두엽, 중심역에서 유의하게 감소된 소견을 보였으며, 델타파에 있어서는 전두-측두엽, 두정-후두엽, 중심역 등 다양한 영역에서 증가 소견을 보여, 같은 나이의 대조군에 비해 유의하게 미성숙한 뇌파 양상 보여주었다. 2) 양반구간 동시성에 있어서는 환아군이 대조군에 비해, 베타파에서 유의한 감소 소견을 보여 주었고, 쎄타파에서도 일부 영역에서 감소 소견을 보여 주었다. 이러한 양상은 양반구의 기능적 결합도에 문제가 있음을 나타내는 소견으로 판단된다. 3) 양반구간 비대칭도 평가에 있어서는, 환아군에서 우측의 기능 저하로 인한, 비대칭도 증가 소견을 몇몇 영역에서 보여 주었는데, 베타파는 두정-후두엽, 델타파는 두정-후두-측두엽 등에서 였다. 결 론 : 이러한 주의력 결핍/과잉 운동 장애 환아군과 정상 대조군의 차이는 기존의 뇌 영상학 연구 및 신경 심리 연구 결과와 일치하는 소견이며, 뇌파의 미성숙과 우반구 기능이상 가설을 지지하는 소견이라고 생각된다. Objectives : To find out the distinctive topographic EEG profiles of subject with attention deficit hyperactivity disorder comparing with age & sex matched control. Methods : The quantitative electroencephalographies(QEEGs) of 20 drug-native ADHD patients without any other psychiatric diagnosis were analyzed and compared to age and sex-matched controls using spectral analysis. Results : 1) Slow waves(delta and theta) bipolar relative powers of the diverse areas in both hemispheres increased in drug-free ADHD patients, compared to controls. 2) Delta and theta bipolar relative powers of localized centro-temporal areas in left hemispheres increased in drug free ADHD patients compared to controls. 3) Interhemispheric coherence and asymmetry are increased in diverse areas in ADHD patient compared to controls. Conclusions : We confirmed QEEG abnormalities in pure ADHD patients, especially in frontal and parietal areas. These results are compatible with other structural and functional imaging studies of ADHD.