Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

가족체계 진단 척도 개발 및 타당화 연구 : Minuchin의 구조적 가족치료 이론에 기초하여 On the Basis of Minuchin`s Structural Family Therapy Theory

이미옥 대한가정학회 2004 Human Ecology Research(HER) Vol.42 No.3

The purpose of this study was to develop the Family System Diagnosis Scale and to m i n e its reliability and validity The subscales of the questionnaire included scores on seven construct. In order to define constructs accurately, a careful review of Minuchin s writings, the writings of other family therapists, and relevant articles on family interaction was undertaken. A pool of 150 items was given to eight family counselors along with a description of Minuchiin s concepts. The counselors were asked to choose the category each statement fit and to rate the degree of fit using the Spoint scale. Using exploratory factor analysis confirmatory factor analysis and Linear Structural Relatimship(LJSREL.), six subdimensions of individuation and 55 items of FSDS were identified; enmeshmen disengqemant(l6 items), parent coalition generational coalition(6 items), flexibility rigidity(5 items), spouse conflict resolved unresolved(8 items). mother-child cohesion estrangement(l0 items), father-child cohesion estrangement(l0 items). 356 adolescents(ages 13-18), 356 fathers, 356 mothers in Seoul, Busan, Dague, Incheun, Dajeun, Ulsan, and Kwangju were completed the Family system Diagnosis We(FSDS). The reliability of the scale was calculated by Cronbach's a Coefficient and the total α = .94 and the calculation for each factor was .87, .60, .77, .80 and .79 respectively.

목욕요법에 대한 고찰

이미림,옥치상 고신대학교 보건과학연구소 2006 보건과학연구소보 Vol.16 No.-

The purpose of this study is to discuss specifically about some kinds of bath therapy. The basics of bathes have many roles in washing skin's dirty, relaxing conglomerate muscles, lessening stresses, increasing blood circulation and metabolism etc. So many peoples take a bath with full-bath, half-bath and feet-bath etc. The full-bath means soaking totally to all of physical body excepting head in the hot water, the half bath soaking to half of physical body, and the feet-bath soaking to only feet. Although the important aspect of bath is to be returned parts of unhealth states, in aspects on convenience and easiness it is good to bathe feet-bath, half-bath and full-bath in order. But in aspects on making head cool, feet warm, that is, making lower body warm, relaxing lower body and dragging down heat of upper body (especially head), it is good to bathe half-bath, knee-bath and feet-bath in order.

요양시설 거주 노인의 간호서비스 요구도에 대한 연구

이광옥,임미림 이화여자대학교 간호과학연구소 2002 Health & Nursing Vol.14 No.2

The purpose of this study was establish the basic material for assisting in raising the quality of nursing service for nurses in the nursing home. For this purpose it attempted to identify the demand of nursing service of old people resident in the nursing home. The research design was the research study to identify the degree of nursing serivce of old people. This study was conducted for 175 old people resident in 4 the nursing home for the elderly in Kangwon, Kyoggi and Kyungbuk provinces and Seoul, able to have the interview with the cognitive ability and understand the purpose of this study and agree to participate in this study. The questionnaire was composed of the following: 16 items relating to the general characteristics; 6 items relating to the physical function; 8 items relating to the daily activity ability; 22 items relating to the cognitive function; one item relating to subjective vision; one item relating to subjective hearing; 71 items relating to the classification of nursing service areas; 71 items relating to the degree of service demand; and 71 items relating to the level of service satisfaction. The contents of the questionnaire were composed by referring to the Psychiatric Nursing Assessment Protocol(Abraham, 1989; Abraham et al,. 1990) and the measurement instrument used by Lee, Kwang-ok. In data collection, research assistants were educated to concerning the structured questionnaire for one day and trained to conduct the interview for the questionnaire research in person. After full discussion over the questionable contents, 175 old people with the cognitive ability were randomly sampled from one ‘cost’ nursing home for the elderly in Seoul, one paid nursing home in Kyonngi Province, one free-charging nursing home in Kangwon Province and each of free-charging, cost and paid nursing homes in Kyungbuk Province. The trained research assistants conducted the questionnaire research through the direct interview. As to the questionable contents, accuracy was confirmed about the questionnaire by having the communication through the personal visit and telephone call with the researcher as needed. In data analysis, data were analyzed using the SAS statistical program after the process of coding. Data concerning the general characteristics of the old people in the nursing home analyzed on the basis of frequency and percentage. And mean and standard deviation were determined concerning the degree of demand for nursing service and Pearson correlation coefficient was used to determine the correlation between principal variables. The degree of demand for nursing service among the old people in the nursing home was shown as follows: The items relating to direct nursing services showed the high average score in venous injection(2.00, rectal injection(2.2), physical treatment(2.5) cold-warm therapy(2.0), vitality symptom measurement(2.1). The items relating to supportive nursing service demonstrated the following high average scores: education for health promotion(2.2); nutritional education(2.2); drug abuse and disuse education(2.5); informing all kinds of equipment and their usage(2.0); asking and counseling with the doctor(2.2); treatment and management plan(2.2); counseling with patients and heir guardian(2.2); assistance in money management(2.1); counseling on the health status92.3); tooth brushing(2.2); shampooing(2.2); bathing(2.4); sitz bath(2.0); assistance in shopping(2.1); recreation(2.1); conversational partner(2.5), emotional support(2.3), assistance in going to hospital(2.3), listening to the family problem(2.0); assistance in clothing and food life(2.2) and referral to the other medical institutions(2.2); Safety and environmental management(2.0) and contagion management(2.0) and the like. The old people showed the high level of demand for nursing services in many items relating to supportive nursing services as well as direct nursing services.

뇌졸중 후 치매환자에서 도네피질(아리셉트^(?))이 인지기능 및 일상생활 수행능력, 삶의 질에 미치는 효과

이상열,장혁,송재은,조성옥,류정미,이미경,조진호,신용일 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.1

Background: This study evaluated the efficacy of administering Donepezil(Aricept^(?)) for improving cognitive function, activities of daily living, and quality of life in patients with post-stroke dementia. Methods: Patients(n=99; mean age, 69.89 years; 48.5% men) with post-stroke dementia, according to criteria of the DSM-IV and MRI, were treated with donepezil 5mg/day or 10mg/day during 24 weeks. They were examined using Mini-mental Status Examination(MMSE), Chinical Dementia Rating(CDR), Barthel Activities of daily living(B-ADL), Instrumental Activities of daily living(I-ADL), Short Form 36 Health Survey-Korean(SF-36-K), and Geriatric Depression Scale(GDS) at before treatment and 24 weeks after treatment. Results: The 97 patients completed the trial. At 24 weeks, they showed significant improvement in MMSE(p<0.001), CDR(p<0.001), B-ADL(p=0.002), I-ADL(p<0.001), and SF-36-K(p=0.003) except GDS(p=0.288). Donepezil was well tolerated. There was no withdrawal due to adverse events. Conclusion: This results demonstrate that donepezil was beneficial in improving the cognitive function, activities of daily living, and quality of life in patients with post-stroke dementia and was well toleraetd.

비만과 자세 안정성 한계

이옥진,김미예 한국스포츠심리학회 2003 한국스포츠심리학회지 Vol.14 No.4

이 연구의 목적은 비만한 사람들의 자세 제어 능력의 부족에 관한 경험적 증거를 얻는 것이었다. 이를 위해서 비만 여대생(15명)과 정상체중 여대생(15명)에게 전, 후, 좌, 우 방향으로 기울여서 버티는 과제를 수행시켰다. 그리고 지면 반력기를 이용하여 압력 중심의 y축과 x축의 최대값, 최소값을 산출하여 그 절대값을 전, 후, 좌, 우 방향의 자세 안정성 한계의 측정치로 사용하였다. 비만 여부와 기울이기 방향에 대한 반복측정 이원분산분석을 실시한 결과, 첫째, 좌우 방향의 안정성 한계가 전후 방향의 안정성 한계보다 컸다. 둘째, 비만 집단이 정상체중 집단보다 안정성 한계가 작았다. 특히, 비만 집단과 정상체중 집단간의 차이는 전방 안정성 한계와 오른쪽 안정성 한계에서 나타났다. 즉, 비만 집단은 정상체중 집단과 비교해서 앞으로 기울이기아 오른쪽으로 기울이기 능력에서 결함을 나타냈다. 이러한 연구 결과는 비만한 사람들의 자세 유지 능력의 부족을 의미하며, 비만과 자세 제어에 관한 추후 연구를 제안하였다. The purpose of this study was to verify difference in limits of stability between the obese and normal weight female adults during leaning forward, backward, right, and left. Using the ground reaction force platform, maximum and minimum values of center of pressure(COP) x and y were calculated. Absolute values of theses were used as a manipulative measures of limits of stability. Analyses of data revealed that 1) limits of stability of medial-lateral lean was significantly wider as compared that of anterior-posterior lean. 2) limits of stability of the obese group was significantly narrower as compared that of the normal weight group. Especially, in forward and right-side lean there was significant difference between the obese and normal weight group. These results suggested that the obese had poor ability of postural control.

성직자 음성의 음향학적인 비교 연구

이은선,박상희,조성미,정옥란,석동일 한국음성과학회 2003 음성과학 Vol.10 No.3

This study compared the voices of ministers and priests. There has been a common notion that ministers are more passionate than priests in delivering their speech. Therefore, it can be assumed that ministers abuses or misuses his/her voice compared to priests. This study attempted acoustic analysis of the choices of 6 ministers and 5 priests before an after their speech. We measured F0, jitter, shimmer, NNE and HNR using Dr. Speech(version 4.0, Tiger DRS). A t-test was performed to determine any objective differences of their voices. The results showed that there were no significant differences in the voices of ministers and priests before an after their speech. However, there seemed to e an interesting reversed tendency between ministers and priests, although it did not reach a statistical significance. That is, F0 tended to increase after the speech in ministers, whereas it tended to decrease in priests. In addition, HNR tended o decrease after the speech in priests, while it tended to increase in ministers.

두피에 발생한 단발성 피지낭종 1예

이민숙,김미연,김형옥,박영민 대한피부과학회 2003 大韓皮膚科學會誌 Vol.41 No.12

A 63-year-old-man presented with an asymptomatic, solitary, yellowish papule on the scalp for one year. There was no family history of similar lesions. A punch biopsy taken from the papule Showed an oily yellowish discharge. Histopathological findings showed a thin-walled cyst lined by a stratified squamous epithelium with hyaline cuticle and no granular layer. Based on this histologic finding, we diagnosed this case as a steatocystoma simplex occurring on the scalp, an unusual site. (Korean J Dermatol 2003;41(12):1657-1658)

유근피가 고콜레스테롤식이를 급여한 흰쥐의 지질대사에 미치는 영향

이미정,하진옥,이재준,이명렬 朝鮮大學校 附設 醫學硏究所 2009 The Medical Journal of Chosun University Vol.34 No.1

연구배경: 유근피는 기능성식품의 신소재로 개발하기 위해서 체계적인 식품학적 연구 및 생리활성효능의 검증이 요구된다. 본 연구에서는 유근피 에탄올 추출물(UDEE)이 고콜레스테롤식이를 급여한 흰쥐의 지방대사 개선효과에 미치는 영향을 구명하고자 혈청 및 간 중의 지질 조성 변화를 검토하였다. 방법: 5주령의 Sprague Dawley계 웅성 흰쥐에게 1% 콜레스테롤과 0.25% sodium cholate를 함유한 고콜레스테롤식이를 조제하여 6주간 급여하였다. 실험군은 정상식이군(NOR), 고콜레스테롤식이군(대조군, CON), 정상식이와 유근피 에탄올 추출물 저용량 투여군(UD1), 정상식이와 유근피 에탄올 추출물 고용량 투여군(UD2), 고콜레스테롤식이와 유근피 에탄올 추출물 저용량 병합 투여군(UDC1) 및 고콜레스테롤식이와 유근피 에탄올 추출물 고용량 병합 투여군(UDC2)의 6군으로 나누어 실시하였다. 유근피 에탄올 추출물은 매일 같은 시각에 경구 투여한 후 흰쥐의 혈액과 간장 내의 지질 조성을 관찰하였다. 결과: 체중증가율과 간 무게는 CON군이 NOR군에 비하여 높게 나타났으나, 고콜레스테롤식이와 유근피 추출물을 병합 투여한 UDC1군과 UDC2군에서 점차 감소하는 경향을 보였다. 혈청 중 ALT, AST 및 ALP 활성은 CON군에서 유의적으로 증가하였고, UDEE 투여로 농도 의존적으로 감소하였다. UDEE 투여군에서는 CON군에 비하여 HDL-콜레스테롤/총콜레스테롤, HDL-콜레스테롤 및 인지질 함량은 증가하였으나 중성지질, 총콜레스테롤, 유리콜레스테롤 및 LDL-콜레스테롤 함량은 감소하였다. 중성지질, 인지질, LDL-콜레스테롤, HDL-콜레스테롤 및 유리콜레스테롤 함량은 정상식이를 급여한 군들 간에는 유의차가 없었다. 또한, 간 중 중성지질과 총콜레스테롤 함량도 UDEE 투여군이 CON군에 비하여 낮았다. 결론: 유근피 에탄올 추출물은 혈청 및 간의 콜레스테롤 함량을 저하시켜 고콜레스테롤혈증 예방과 치료에 효과적일 것으로 판단되어진다. Background and Objectives: This study was performed to investigate the effect of ethanol extract of Ulmus davidiana var. japonica Nakai(UDEE) on serum and liver lipid metabolism in rats fed high cholesterol diet. Methods and Materials: Male Sprague-Dawley rats were administered 1% cholesterol and 0.25% sodium cholate to induce hypercholesterolemia. We divided into six groups; normal diet group(NOR), high cholesterol diet group(CON), normal diet and UDEE 200 mg/kg administered group(UD1), normal diet and UDEE 400 mg/kg administered group(UD2), high cholesterol diet and UDEE 200 mg/kg administered group(UDC1), and high cholesterol diet and UDEE 400 mg/kg administered group(UDC2). Results: The growth rate and liver weight of the CON group was higher than the NOR, whereas the administered UDEE groups were gradually decreased. There was a significant increase in the activities of serum ALT, AST and ALP in CON group. The administration of UDEE decreased serum ALT, AST and ALP activities in dose dependently manners. UDEE administered groups were increased HDL-C/T-C, HDL-cholesterol and phospholipid levels, and decreased serum triglyceride, total cholesterol, free cholesterol, and LDL-cholesterol levels as compared with CON group. There were no differences in the levels of serum triglyceride, phospholipid, LDL-cholesterol, HDL-cholesterol and free cholesterol between normal diet groups. The hepatic levels of total cholesterol and triglyceride were also lower in UDEE administered groups than in CON group. Conclusion: These results suggest that UDEE exerts hypocholesterolemic effect by reducing serum and liver cholesterol contents.