Disruption of rsmA Gene of Pectobacterium carotovorum subsp. carotovorum LY34 and Effect on Pathogenicity

Kim, Min Keun,Kang, Tae Ho,Kim, Sung Kyum,Jeong, Yu Seok,Yun, Han Dae,Kim, Hoon The Korean Society for Applied Biological Chemistr 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.6

The rsmA gene was cloned from soft-rot bacterium Pectobacterium carotovorum subsp. carotovorum LY34 (Pcc LY34), and its role in pathogenicity was investigated by marker exchange mutagenesis. From a cosmid library of Pcc LY34 genomic DNA, a positive clone carrying the rsmA gene was selected, and the gene was cloned by polymerase chain reaction (PCR) amplification. The gene is 186 bp in size and encodes a protein of 62 amino acids with a predicted molecular mass of 6,839 Da. The calculated pI of the RsmA is 8.16. The phylogenetic tree showed that the RsmA of Pcc LY34 appeared genetically identical to the CsrA of Pectobacterium atrosepticum SCRI1043 (100% identity) and similar to the CsrA of Yersinia pestis KIM10+(98.3%). The gene was disrupted by the $Km^r$ gene, and the cells became mutated (i.e., $RsmA^-$ mutant). The pathogenicity test revealed that the disease rating of the $RsmA^-$ mutant only differed slightly from that of the wild type on a slice of potato tuber and a Chinese cabbage stalk. These results suggest that RsmA is not an essential factor for the pathogenicity of Pcc LY34 and that the rsmA gene of Pcc LY34 is not completely derepressed in the $RsmA^-$ mutant for virulence-related genes, contrary to the results of Erwinia carotovora subsp. carotovora $RsmA^-$ mutant, which proved hypervirulent for celery petioles. These results showed that the microenvironmental conditions of the host and/or strain of pathogen are important for the coordination of virulence gene expression.

Proteomic Approach to Analyzing Flooding Stress in Soybean (Glycine max(L.) Merr.)

Kim,Tae-Seon,Cho,Seong-Woo,Lee,Jae-Eun,Kim,Yook-Han,Cho,Kun,Choi,Jong-Soon,Chung,Keun-Yook,Song,Beom-Heon,Jong,Seung-Keun,Kim,Hong-Sig,Woo,Sun-Hee 한국작물학회 2005 한국작물학회 학술발표대회 논문집 Vol.2005 No.-

Misorientation Characteristics at the Growth Front of Abnormally-Growing Goss Grains in Fe–3%Si Steel

Tae‑Young Kim,Tae‑Wook Na,Hyung‑Seok Shim,Yong‑Keun Ahn,Yong‑Kwon Jeong,Heung Nam Han,Nong‑Moon Hwang 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12

Penetrating morphologies at the growth front of abnormally-growing Goss grains in Fe–3%Si steel was examined in view ofthe possibility that they are the two-dimensional section of the solid-state wetting (SSW) along the triple junction line. Toobtain the statistically meaningful data, 102 penetrated grain and 204 penetrating abnormally-growing Goss grain morphologieswere examined. The misorientation angles of penetrating and penetrated grains were measured by electron backscattereddiffraction. Among the 102 examined penetrated grain boundaries, none has low misorientation angles less than 15°,whereas 17.2% of the 204 penetrating grain boundaries have low misorientation angles, and 23.5% of them have coincidencesite lattice boundaries. Besides, boundary energies of penetrating Goss grains, which were estimated from misorientationangles of the three grains in the penetrating morphology, satisfied the energetic condition for SSW along the triple junctionline. These results imply that the abnormal grain growth of Goss grains in Fe–3%Si steel occurs by the mechanism of subboundaryenhanced SSW.

목재가 선적된 선창에서 발생한 산소결핍에 의한 질식사

김동훈,김기권,장태정,김정란,이구,김수근,임현술 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

Oxygen deficiency has been frequent in a clouted space. Wood consumes oxygen and discharges carbon dioxide Instead of photosynthesis in closed space without light, so do some microorganisms on the surface. We experienced a case that a healthy Insect-proofer fell down and died of asphyxia on stair-board at 7 m below the hatch of the cargo-hold shipping wood Analysis of gases in cargo-hold revealed O2; 12.3%, CO; 105 ppm, CH4; 2.7%, and H2S; 1.9% at 1 m below the hatch, and then O2; 6.1%, CO; 220 ppm, CH4; 2.9%, and H2S, 2.3 ppm at 2.5 m below the hatch. Autopsy findings were unremarkable. We justiced the cause of death asphyxia due to oxygen deficiency. As seen in this case, the serious oxygen deficiency was accounted for oxygen consumption by wood and microorganisms.

AHP를 이용한 CT 및 MRI의 비용편익분석

조근태,김성조,김성민,김용우,김성재,조용곤 한국경영과학회 2004 經營 科學 Vol.21 No.2

The imports of used medical equipment such as CT, MRI have been rapidly increased every year. How to manage those medical equipment has been one of critical issues in the government sector. In this study, we demonstrate how benefit/cost analysis using the Analytic Hierarchy Process (AHP) can be applied to the comparison of used CT/MRI and new CT/MRI. The final results show that the new CT/MRI is more attractive than the used CT/MRI.

Pollen Viability of Common Buckwheat (Fagopyrum esculentum Moench.) in in vitro Germination and its Application to Male Gametophyte Isolation

Woo, Sun-HeeㆍKeun-Yook ChungㆍHeung-Tae, KimㆍYong-Gu, ChoㆍHong-Sig, KimㆍBeom-Heon, Songㆍ Chul-Won, Lee and Seung-Keun Jong 忠北大學校 農業科學硏究所 2004 農業科學硏究 Vol.21 No.-

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학술연구비지원정책의 효과에 관한 실증적 분석 : 연구비지원방식과 연구결과물 수준의 관계를 중심으로

김태일,남궁근 한국정책학회 2003 韓國政策學會報 Vol.12 No.2

본 연구의 목적은 한국학술진흥재단의 연구비 지원방식과 연구논문 수준과의 관계를 분석하는 데 있다. 이를 위한 연구질문은 다음의 네 가지이다. ①연구자의 과거 연구업적이 우수할수록 연구결과물의 수준이 높은가? ②과제당 연구지원금액의 증가는 연구결과물의 수준을 향상시키는가? ③'다수소액과제'지원에서 '소수다액과제'지원으로의 변화는 연구결과물의 질을 향상시키는가? ④연구비 규모가 큰 공동연구과제는 규모가 작은 개인연구과제에 비하여 우수한 연구결과물을 산출하는가? 이를 규명하기 위하여 1996년부터 1999년까지 학술진흥재단에서 지원한 자유공모, 선도연구자, 신진연구자, 협동연구, 연구소 과제 8,511개 중에서 1,600여 과제를 선정하여 연구결과물 수준, 연구업적, 지원금액 등의 정보를 사용하여 기술통계 및 회귀분석을 실시하였다. 분석결과는 다음과 같다. ①연구자의 논문실적과 연구결과물의 수준 사이에는 양의 상관관계가 있으며, 이는 공동 연구보다 개인연구에서 더 뚜렷하다. ②개인과제는 공동과제에 비하여 연구결과물 수준이 대체로 더 우수하다. ③개인연구과제의 경우 지원금액과 연구결과물 수준은 별 관계가 없는 것으로 판단된다. ④자연·공학 분야 공동연구관제의 경우 지원금액이 많을수록 연구결과물 수준이 높아지는 경향이 있다. This paper analyses the relations between the quality of research and the method of grant allowance by Korea Research Foundation. To do this, we examine following issues: 1) the relations between the quality of research and the researcher's past research results: 2) the relations between the quality of research and the amount of grant: 3) the relations between the quality of research and the changes of policy from "small grant to many researchers" to "large grant to selective researchers": 4) the comparisons of research quality between the individual research and the joint research. The results are as follows: 1) there exist positive correlations between quality of research and the researcher's past research results: 2) no clear relations exist between quality of research and amount of grant in individual researches, however, some positive correlations exist between research quality and grant amount in joint researches of the natural science and engineering fields: 3) the quality of individual research is superior to the quality of joint research in general.

유리체절제술중 발생한 의인성망막열공

김용백,박근성,홍성태 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

To determine the current incidence, location and treatment of intraoperative iatrogenic retinal breaks occuring during pars plana vitrectomy, we reviewed the records of 208 consecutive eyes after pars plana vitrectomy. Thirty seven of 208 eyes (18%) developed 68 intraoperative tears. Of the 68 tears, 14 (21%) were located in the anterior to equator and 54 (79%) were located in the posterior to equator. Thirty six retinal tears (43%) were located in the upper quadrants and in the lower quadrants, we detected 47 retinal tears (57%). Patients with proliferative diabetic retinopathy and proliferative vitreoretinopathy had a higher incidence of iatrogenic retinal breaks than those with other diagnoses. Methods used to treat the retinal tears included cryotherapy, laser photocoagulation, scleral buckling, internal tamponade of gas, air and silicone oil. Thirty three of 37 eyes (89%) were reattached. According to the use of endolaser photocoagulation, we divided the period of study that the first half : 1989. 5. - 1991. 6. and the second half : 1993. 1 - 1993. 12. The results of chorioretinal adhesion in the 1st half were higher than those of the second half. These results show that vitreoretinal surgery for proliferative diabetic retinopathy and proliferative vitreoretinopathy requires careful managements for prevention of iatrogenic retinal tears. Despite improvements in instrumentation and surgical techniques, iatrogenic retinal tears continue to be an important complication of pars plana vitrectomy. However, with careful intraoperative examination, most iatrogenic retinal tears can be identified and treated successfully.

동일 전압불평형률 상태에서 유도전동기의 불평형전류 분석

김태한,좌종근 제주대학교 공과대학 첨단기술연구소 2004 尖端技術硏究所論文集 Vol.15 No.2

The unbalanced currents of an induction motor which is applied by various unbalanced voltages are analyzed under same unbalance voltage factor condition. The current unbalance factors of full load, a half load, and no load of the motor are computed with the unbalanced currents of transient and steady state. For these computation, the dynamic equations of the motor operation are derived by the dq coordinate system and simulated using MATLAB/SIMULlNK. It is found that the unbalance rate and condition of currents are different between the transient and steady state.

골다공성 척추 압박골절 환자에 대한 경피적 척추체성형술 : 최근 2년간 경험 Recent 2-year Experience

김상대,박정율,김세훈,서중근,이태수,채수원 대한척추신경외과학회 2004 Neurospine Vol.1 No.1

Objective: To describe the therapeutic outcome of percutaneous vertebroplasty for the treatment of osteoporotic vertebral compression fractures(OVCF) in recent years with review of literature. Methods: The authors have retrospectively reviewed a consecutive group of patients who underwent percutaneous vertebroplasty in our institution between May 2000 and October 2002. The procedures were performed with Jamshidi needle via transpedicular approach followed by injection of polymethyl- methacrylate(PMMA) into the involved vertebral bodies. Prolo's economic and functional rating scale was used for therapeutic outcome assessments. Results: There were 91 patients(69 female, 22 male) with OVCF, with involvement of 147 vertebral bodies. The mean age was 72 years. The average BMD t-score was -3.78, indicative of severe osteoporosis. Minimal follow-up period was three months. And among these patients, 82.5% of patients were maintained over good condition. Extrusion of PMMA cement beyond the confines of the vertebral body was obseved in 13 cases(8.8%). Although most of them improved with conservative treatments without morbidities, two developed serious complications, one being pulmonary embolism and the other paraparesis. Conclusion: Percutaneous vertebroplasty is one of the effective minimal invasive treatment modalities in OVCF. However, proper biomechanical studies supporting these procedures are not fully evidenced. Serious complications, although rare, should be prevented with the aid of improved techniques and newer biocompatible materials along with better understanding of biodynamic and biomechanical impact on normal and already compromised vertebraes.