Clear cell sarcoma of the kidney : Immunohistochemical study and flow cytometric DNA analysis of 7 cases

Choi, Yoon-Jung,Jung, Woo-Hee,Jung, Soon-Hee,Park, Chanil Yonsei University, College of Medicine 1994 Yonsei medical journal Vol.35 No.3

축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

급성전골수성백혈병에서 백혈구성분채집술에 이은 치명적인 뇌출혈 2예

오윤정,박소윤,김윤정,한동석,김현수,최진혁,남동기,임호영,김효철,주희재 아주대학교 의과학연구소 1998 아주의학 Vol.3 No.1

The aim of this study is to report 2 cases of acute promyelocytic leukemia who died from intracranial hemorrhage following leukapheresis and to provide proper preventive measures against hemorrhage following leukapheresis. From 1994 to 1997, a total of twenty-six patients with leukemia underwent leukapheresis to control hyperleukocytosis at Ajou University Hospital. Two patients with acute promyelocytic leukemia received all-trans retinoic acid but developed drug-induced hyperleukocytosis. Shortly after leukapheresis, they died from intracranial hemorrhage. The risk factors for fatal hemorrhage are thought to be coexisting disseminated intravascular coagulation(DIC), thrombocytopenia aggravated by leukapheresis, exacerbated coagulopathy related to mechanical trauma through leukapheresis and the excess use of citrate during leukapheresis. To reduce the risk of the bleeding associated with leukapheresis, it is necessary to replace platelet sufficiently before and after leukapheresis and to give calcium to correct coagulopathy induced by excess citrate which is used as anticoagulant as well as the correction of disseminated intravascular coagulation.

Insulin resistance mediates high-fat diet-induced pulmonary fibrosis and airway hyperresponsiveness through the TGF-β1 pathway

Park, Yoon Hee,Oh, Eun Yi,Han, Heejae,Yang, Misuk,Park, Hye Jung,Park, Kyung Hee,Lee, Jae-Hyun,Park, Jung-Won Nature Publishing Group UK 2019 Experimental and molecular medicine Vol.51 No.5

<▼1><P>Prior studies have reported the presence of lung fibrosis and enhanced airway hyperresponsiveness (AHR) in mice with high-fat-diet (HFD)-induced obesity. This study evaluated the role of TGF-β1 in HFD-induced AHR and lung fibrosis in a murine model. We generated HFD-induced obesity mice and performed glucose and insulin tolerance tests. HFD mice with or without ovalbumin sensitization and challenge were also treated with an anti-TGF-β1 neutralizing antibody. AHR to methacholine, inflammatory cells in the bronchoalveolar lavage fluid (BALF), and histological features were evaluated. Insulin was intranasally administered to normal diet (ND) mice, and in vitro insulin stimulation of BEAS-2b cells was performed. HFD-induced obesity mice had increased insulin resistance, enhanced AHR, peribronchial and perivascular fibrosis, and increased numbers of macrophages in the BALF. However, they did not have meaningful eosinophilic or neutrophilic inflammation in the lungs compared with ND mice. The HFD enhanced TGF-β1 expression in the bronchial epithelium, but we found no differences in the expression of interleukin (IL)−4 or IL-5 in lung homogenates. Administration of the anti-TGF-β1 antibody attenuated HFD-induced AHR and lung fibrosis. It also attenuated goblet cell hyperplasia, but did not affect the AHR and inflammatory cell infiltration induced by OVA challenge. The intranasal administration of insulin enhanced TGF-β1 expression in the bronchial epithelium and lung fibrosis. Stimulating BEAS-2b cells with insulin also increased TGF-β1 production by 24 h. We concluded that HFD-induced obesity-associated insulin resistance enhances TGF-β1 expression in the bronchial epithelium, which may play an important role in the development of lung fibrosis and AHR in obesity.</P></▼1><▼2><P><B>Obesity: A trigger for asthma onset</B></P><P>Insulin resistance may be an important causative factor underlying the increased risk of asthma and other respiratory issues in obese individuals. Obesity doubles the likelihood of developing asthma, with symptoms that are more difficult to control than in non-obese patients. The connection between these conditions is poorly understood, but researchers led by Jung-Won Park, Yonsei University College of Medicine, Seoul, South Korea, have identified a potential mechanism. They demonstrated that a signaling molecule called TGF-β1 contributes to airway sensitivity and tissue scarring in a mouse model of diet-induced obesity. Subsequent experiments showed that treatment with insulin also gives rise to increased TGF-β1 production in the mouse lung. Since insulin resistance is a common feature of obesity, resulting in abnormally high levels of circulating insulin, this could also account for the increased risk of respiratory problems.</P></▼2>

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

실험동물에서 주야시차가 Bromobenzene의 대사효소계에 미치는 영향

정운용,윤병재,최종원,천현욱,조희숙 慶星大學校 環境問題硏究所 1999 環境硏報 Vol.9 No.1

To investigate the circadian variation in the bromobenzene metabolism, bromobenzene(480 mg/kg body weight) was intraperitoneally administeres to the rats every other day for 6 days both in the night: 24:00 and day: 12:00. Each group of animals were sacrificed at 8hr after last injection of bromobenzene. Activities of cytochrome p-450, aminopyrine demethylase and aniline hydroxylase as free radical generating enzymes were changed by the night phase than those of day phase but in case of bromobenzene traeatment and the injection of prednisolone inhibitd the hepatic cytochrome 0450 content in rats. Activities of cytosolic xanthine oxidase and aldehyde oxidase as free radical generating enzymes were changed by the night phase than those of day phase but in case of bromobenzene treatment. Futhermore, the decreasing rate of hepatic glutathione level to the control was higher in rats of day phase then those of night phase by the bromobenzene treatment and the injection of prednisolone inhibited the hepatic glutathione content in rats. And the hepatic glutathione S-transferase activity was increased bromobenzene treated rats of the night phase than those of day phase and the injection of prednisolone inhibited the hepatic glutathione S-transferase activity in rats. And the hepatic lipid peroxide content was increased bromobenzene treated rats of the night phase than those of day phase. On the other hand, liver weight per body weight(g), serum level of aminotransferase, sorbitol dehydrogenase, alklaline phosphatase, lactate dehydrogenase and γ-glutamyltransferase were more increased both in bromobenzene-treated and control rats of the night phase than those of day phase. These result indicate that the rats of night phase may induce more accelerated formation of bromobenzene 3,4-oxide from bromobenzene than those of day phase in rats.

유지의 종류와 2-Acetylaminofluorene 투여가 흰쥐의 혈청 지질조성에 미치는 영향

윤혜진,김정희 서울여자대학교 자연과학연구소 1996 자연과학연구논문집 Vol.7 No.-

지방산의 종류와 발암물질의 투여는 흰쥐의 혈청 중성지질이나 cholesterol 함량 등에 다른 영향을 미치고 있음이 알려져왔다. 그러나 아직도 그 기전에 대해서는 확실히 밝혀지고 있지 않은 실정이므로 본 연구에서는 우지, 옥수수유, 들깨유를 사용하여 발암물질을 투여하고 11주간 사육한 후에 혈청 지질 조성이 어떻게 영향을 받는지를 연구하였다. 1. 체중은 우지군이 옥수수유군이나 들깨유군보다 유의성 있게 높았으며, 발암물질을 투여한 군들에서 유의성 있게 낮아졌다. 체중당 간무게로 환산했을 때 발암물질 투여에 의하여 유의성있게 증가되었다. 2. 혈청의 중성지방 함량은 들깨유군이 우지군에 비하여 유의적으로 감소하였으며 그 순서는 우지군, 옥수수유군, 들깨유군의 순으로 낮았다. 3. 혈청의 cholesterol 함량은 들깨유군이 우지군이나 옥수수균에 비하여 유의성 있게 낮았다. 4. 발암물질 투여에 의하여 우지군, 옥수수유군에서는 혈청중성지방이나 cholesterol 함량에 유의적인 감소가 있었으나, 들깨유군에서는 유의적인 차이를 볼 수 없었다. 5. 혈청의 HDL-cholesterol의 함량은 유지의 종류와 발암물질의 투여 여부에 따른 유의적인 차이를 볼 수 없었다. 6. 혈청의 인지질 함량은 우지군, 옥수수유군, 들깨유군의 순으로 낮아졌으나 발암물질 투여에 있는 유의적인 차이는 볼 수 없었다. 그러므로 n-3 지방산인 들깨유의 섭취는 혈청 cholesterol 양이나 중성지질의 양을 감소시킴에 따라 심장순환계의 예방 식이로 바람직함을 알 수 있었고 아울러 발암물질 투여시 혈청 지질대사에서 다른 유지와는 다른 영향을 미치는 것을 알 수 있었으므로 암예방에 좋은 효과를 기대할 수 있을 것으로 사료된다. Number of studies revealed that treatments of different dietary oil and carcinogen affect differently on the serum composition of rats. Male Sprague-Dawley weaning rats were fed on semi-synthetic diets containing 15% beef tallow(BT), corn oil (CO) or perilla oil(PO). Three weeks later, the half of rats in each diet group were injected with a single dose of 2-acetylaminofluorene (2-AAF 50mg/kg body weight) as a carcinogen. The injection was conducted 3 times on every other days. After 11 weeks of experimental diet, the present study examined both effects of the different dietary fats and the carcinogen injection on serum lipid levels. The body weight of BT groups was higher than that of either CO or PO groups and it decreased with the carcinogen treatment. Also, the liver weight per body weight significantly increased with the carcinogen treatment. The serum triglyceride level of PO groups was significantly lower than that of BT groups. The serum total cholesterol level was also significantly lower in PO groups than other two gruops. Interestingly by carcinogen treatment, serum TG and cholesterol levels were significantly decreased in BT or CO groups whereas not significantly decreased in PO groups. However, the serum HDL-cholesterol level was not affected by either different dietary oil or carcinogen treatment. The serum phospholipid levels of PO groups were also significantly lower than that of BT groups. However, it was not affected by carcinogen treatment. Overall results suggest that PO as a source of n-3 fatty acids have a lowing effect on serum cholesterol, triglyceride, and phospholipid levels. In addition, PO feeding differs from BT or CO feeding in terms of the change in serum lipid levels during hepatocarcinogenesis.

몇가지 소독약의 향균작용에 대한 실험적 검토

정병기,조윤희 中央醫學社 1971 中央醫學 Vol.20 No.3

Since the antimicrobial activities of disinfectants are known to be modified by the various factors, it was thought worthwhile to investigate the activities of some commercially available disinfectants under the varied conditions. Thus, the activities of disinfectants A,B,C,D, and E, all of which may be purchased at the drug-stores, to the stock and freschly isolated strains of Staph. aureus, Es. coli and Ps. aeruginosa were investigated at the environmental temperatures of 5°C, 20°C, and 30°C. The results of observation might be summarized as follows: 1) The antibacterial effects of disinfectants A were more powerful in lower temperature than in higher temperature tested. After 15 minutes of exposure at 5°C. to the 1 : 250 dilution, it appeared to have caused the loss of viability of all the strains. 2) The antibacterial effects of disinfectant B increased with the elevation of temperature. After 10 minutes of exposure at 30°C. to the 1 - 625 dilution, it appeared to have caused the loss of viability of all the strains. 3) Under the experimental conditions employed, disinfectant C did not show any antibacterial activity. 4) The antibacterial activity of disinfectant D was found stronger at 20°C, or 30°C, than at 5°C. After 10 minutes of exposure at 20°C. to the 1 : 120 dilution of the disinfectant, all the strains appeared to have lost the viability. 5) The antibacterial effects of disinfectant E (Phenol) increased with the elevation of

옥수수유 및 정어리유 섭취가 발암물질을 투여한 흰쥐에서의 혈청 중성지질 및 콜레스테롤 함량에 미치는 영향

윤혜진,김정희 서울여자대학교 자연과학연구소 1995 자연과학연구논문집 Vol.6 No.-

지방산의 종류는 암의 생성과정 뿐만 아니라 암화과정의 억제 및 촉진에 다른 영향을 미치고 있음이 알려져왔다. 그러나 아직도 그 기전에 대해서는 확실히 밝혀지고 있지 않은 실정이다. 그러므로 본 연구에서는 n-3 지방산인 정어리유 섭취시에는 n-6 지방산인 옥수수유와는 다른 영향을 미칠 것이라고 생각되므로 발암물질을 투여하여 12주간 사육하는 동안에 4, 6, 8, 12주에 각각 sampling을 하여 혈청 지질조성이 어떻게 영향을 받는지를 연구하였다. 1. 체중은 옥수수유군 보다 정어리유군에서 높은 경향을 보였고 발암물질 투여시에는 감소되는 경향을 보였으며 특히 발암물질 투여시 간의 비대화와 섬유화가 대부분의 섬유화가 대부분의 실험쥐에서 관찰 되었다. 2. 혈청의 중성지방 함량은 유지의 종류와 발암물질의 투여 여부에 따른 현저한 차이를 보였는데, 정어리유군에서 옥수수유군 보다 유의성있게 낮았으면 발암물질 투여군에서 낮아졌다. 분석시기에 따른 변화는 4주에서는 정어리유군에서 옥수수유군 보다 유의성 있게 낮았고 발암물질 투여에 의하여 낮아지는 경향을 보였으며, 6주군에는 군간의 유의적인 차이를 보이지 않았고, 8주에는 SO군에서 감소되었고 CO-CAR군에서는 유의적으로 증가되었으며, 12주에는 CO군만이 유의성 있게 증가되었다. 3. 혈청의 콜레스테롤 함량은 정어리유군에서 유의성 있게 낮았으며 발암물질 투여에 의한 차이는 6주와 8주에는 발암물질 투여군에서 낮아졌다가 12주에는 반대로 높아지는 경향을 보였다. 분석시기에 따른 변화는 발암물질을 투여하지 않은 군은 6주에서 증가하고 12주까지 계속 감소되는 경향을 보이는데 반해서 발암물질을 투여한 군은 주별로 큰 차이를 보이지 않아 12주에는 발암물질을 투여하지 않은 군에 비하여 오히려 높은 결과를 보여 주었다. 4. HDL-cholesterol의 함량은 옥수수군에 비하여 정어리유군에서 유의성 있게 낮았으나 발암물질 투여에 의한 효과는 6주와 8주에는 발암물질 투여군에서 유의성있게 낮아졌으나 4주와 12주에는 유의적인 차이를 볼 수 없었다. 시기에 따른 변화는 4주에는 정어리유군에서 낮았고, 6주와 8주에는 CO군에서 증가되었으며, 12주에는 발암물질 투여구에서 증가하는 경향을 보여 SO군에서 유의성 있게 낮았다. 이상의 결과를 종합하면 EPA나 DHA를 많이 함유한 정어리유의 섭취는 혈청 cholesterol양이나 중성지질의 양을 감소시킴에 따라 심장순환계 질환의 예방 식이로 바람직함을 알 수 있었고 아울러 발암물질 투여시 혈청 지질대사에 옥수수유와는 다른 영향을 미치는 것을 알 수 있었으므로 암예방에 좋은 효과를 기대할 수 있을 것으로 사료된다. The present study examined effects of sardine oil rich in n-3 fatty acids and corn oil rich in n-6 fatty acides on serum lipid levels in chemically induced hepatocarcinogenesis system. Weanling male Sprague-Dawley rats were fed either 15% corn oil or sardine oil. Two weeks after feeding, the rats were given a single intraperitoneal injection of diethylnitrosamine (200mg/kg body weight). From 4 week, rats were given 0.02% 2-acetylaminofluorene(2-AAF) in diet for 4 weeks. At 6 week, 0.05% phenobarbital(PB) was added to the diets until the end of experiment. Samplings were conducted at 4,6,8, and 12 week. Body weight of sardine oil (SO) group tends to be higher than that of corn oil (CO) group at all sacrifice time although the effect was not significant. However the body weight was decreased by carcinogen treatment. Serum triglyceride (TG) level of SO group was lower than that of CO group at 4,8 and 12 week. By carcinogen treatment, the serum TG level was significantly increased at 8 week but decreased at 12 week. Such effect was greater in CO feeding group. Serum total cholesterol level of SO group was also lower than that of CO group at all weeks. The change pattern for serum total cholesterol level by DEN, 2-AAF and PB treatment was similar irrespective of dietary oil. Interestingly at 8 week serum total cholesterol level was significantly decreased by carcinogen treatment in CO feeding group while not significantly decreased in SO feeding group. However at 12 week serum total cholesterol level was significantly increased by carcinogen treatment. Serum HDL-cholesterol levels of So group were lower than that of CO group. Serum HDL-cholesterol level of carcinogen untreated group was increased up to 6 week, and then remain constant after that. But HDL-cholesterol level of carcinogen treated group was lower than that of carcinogen untreated group at both 6 and 8 week and then increased at 12 week. Overall results indicate that fish oil not only has a lowering effect on both serum cholesterol and triglyceride level but also differs from corn oil in terms of the changes in serum lipid metabolism during hepatocarcinogenesis.