한국성인의 腸內細菌分布에 관한 연구

姜國熙,朴勇河 成均館大學校 科學技術硏究所 1984 論文集 Vol.35 No.2

Quantitative and qualitative examination of the fecal flora of 17 clinically healthy Korean adults were carried out by using anaerobic jar culture technique. Bacteroidaceae, Bifidobacterium, Eubacterium, and Peptococcaceae were the predominant organisms approximating 10^10-10^11 in number per gram dry weight. Enterobacteriaceae, Streptococcus, Lactobacillus, and Clostridium were present in number of 10^7-10^9 per gram dry weight material, while Corynebacterium, Veillonella, and Bacillus were present in lower numbers. The human adult fecal flora was predominantly anaerobic. The aerobes are significantly less than the anaerobes and their numbers varied among specimens.

비피더스균(Bifidobacterium)의 배양과 이용

姜國熙,朴勇河,金尙希 成均館大學校 1983 論文集 Vol.33 No.-

The fact became generally to know that Bifidobacterium were beneficial to human health. One gram of solid faeces(wet weight) was homogenized for not longer than 2 min. in 9ml of anaerobic dilution in glass homogenizer to give a homogeneous suspension and 10-fold dilutions in anaerobic dilutions were made from this suspension. BS, BL, and EG agar medium were used for isolation of Bifidobacterium. 10 Strains of Bifidobacterium were isolated from human faeces. They were identified and examined in physiological character. Concentration of Bifidobacterium was 3.0×10 exp 9/g in the faeces of healthy adults of Korean(19 adults aged 20∼25 years). Concentrations of Bifidobacterium in the faeces of one man were changed in each days.

돼지설사 원인균 E. coli의 생육저해에 대한 유산균의 효과

姜國熙,成文喜,方泰瑛,龍球淵,林讚洙 成均館大學校 科學技術硏究所 1985 論文集 Vol.36 No.1

E. coli A_2 diarrhea causing bacteria of pigs was anaerobically inoculated with 10^5/ml into prereduced YS medium (10% reconstituted skim milk+0.1% yeast extract), and then lactic acid bacteria were inoculated with 10^5/ml and 10^7ml into previous inoculated samples of E. coli A_2. Inoculated samples cultured at 37℃, were tested to determine changes in viable numbers of organisms and pH. The experimental results are summerized as follows: 1. In the mixed culture medium of 10^5/ml E. coli A_2 and L. casei YIT9018, E. coli A_2 decreased after 36 hours from incubation. 2. In the mixed culture medium of 10^5/ml E. coli A_2 and L. acidophilus, E. coli A_2 drastically decreased after 24 hours from incubation and was almost disappeared after 72 hours from incubation. 3. In the mixed culture medium of 10^5/ml E. coli A_2 and L. bulgaricus, E. coif A_2 drastically decreased after 24 hours from incubation and was almost disappeared after 72 hours from incubation. 4. In the mixed culture medium of 10^5/ml E. coli A_2 and Str. thermophilus, E. coli A_2 decreased after 12 hours from incubation. 5. When the pH value of the mixed culture, was, 4. 5 below, E. coli A_2 decreased.

헬리코박터 파이로리 균 진단용 ^13C-요소 캅셀의 개발

용철순,김용일,김지만,강성훈,권기철,이종달,김종국,사홍기,최한곤 영남대학교 약품개발연구소 2002 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-

The purpose of this study was to develop a new ^13C-urea-containing capsule for diagnosis of H.pylori. The urea-containing capsules were prepared with various diluents such as polyethylene glycol (PEG), microcrystaline cellulose, sodium lauryl sulfate and citric acid. The dissolution test, ^13C-urea breath test and stability test were then performed on the capsules. Microcrystalline cellulose and sodium lauryl sulfate retarded the initial dissolution rates of urea. However, PEG increased the initial dissolution rates of urea. Furthermore, two formulae composed of PEG,[^13C-urea/PEG (38/1.9 mg/cap)] and [^13C-urea/PEG (38/1.9/1.9 mg/cap)] had the maximum DOB value. about 16 at 20 min, while the formula composed of only 38 mg ^13C-urea had the maximum DOB value at 30 min. The results indicated that PEG improved the sensitivity of ^13C-urea in the human volunteers. The capsule [^13C-urea/PEG (38/1.9 mg/cap)] was stable for at least six months in 25 and 37℃. Thus, a PEG-containing capsule, [^13C-urea/PEG (38/1.9 mg/cap)] would be a more economical, sensitive and stable perparation for diagnosis of H. pylori.

모 축전지 회사 근로자들의 연폭로 지표에 대한 경시적 관찰

강명식,김용배,이용진,리갑수,김화성,황규윤,장봉기,이성수,안규동,이병국 순천향대학교 산업의학연구소 1998 순천향산업의학 Vol.4 No.1

Lead poisoning is still prevalent and one of important occupational disease in Korea. To prevent undue high lead burden and maintain good health condition of lead workers in lead using industries, periodic health examination and regular biological monitoring of lead workers are often required in addition to the fundamental improvement of their working condition. To evaluate the effect of comprehensive occupational health program with regular biological monitoring on the level of lead exposure of lead workers in lead using industries, 11 year database from 1983 to 1994 of one storage battery factory was reviewed. From 1983 to end of 1986 only periodic health examination was provided to the lead workers, but from 1987 comprehensive occupational health program with regular biological monitoring was provided to maintain better health condition of lead workers. Selected study variables for lead exposure were blood lead(PbB), zinc protoporphyrin in blood(ZPP), δ-aminolevulinic acid in urine(ALAU), hemoglobin(Hb), hematocrit(HCT). Information on sex, age and work duration was also reviewed. 5 years data from 1983 to 1987 and 3 years data from 1990, 1993 and 1994 were available for all lead exposure indices except ALAU which has only 5 years data from 1983 to 1997. The results of 11 years longitudinal observation of lead indices are all follows: 1. The mean value of ZPP of lead workers in 1983 was 148.7±73.1 ㎍/㎗(Male: 145.5±72.3, Female: 168.1±79.1), whereas that of ZPP of lead workers in 1994 was decreased to 64.3±32.7 ㎍/㎗(Male: 60.2±28.8, Female: 95.2±43.4). 2. The mean value of PbB of lead workers in 1983 was 56.4±18.8 ㎍/㎗(Male: 55.59±19.1, Female: 59.6±19.1), whereas that of PbB of lead workers in 1994 was dcreased to 35.4±11.9 ㎍/㎗(Male: 35.7±12.0, Female: 32.8±10.8). 3. The mean value of Hb of lead workers in 1983 was 14.1±1.4 g/㎗(Male: 14.4±1.1, Female: 11.7±0.8), whereas that of Hb of lead workers in 1994 was increased to 15.6±1.5 g/㎗(Male: 15.9±1.2, Female: 12.9±1.1). 4. Blood lead is most highly correlated with ZPP(r=0.522, male:0.54 and female:0.50) and also correlated with ALAU and work duration also. 5. The simple regression analysis of PbB and ZPP showed different coefficient of regression by the measurement year, but there was no difference of coefficient of regression between sex. The slope of equation of pooled data for 1983-1986 without comprehensive occupational health program was 2.16 and differed from that of pooled data for 1987-1994(1.25). This difference suggests that the unit effect of blood lead on ZPP was relatively smaller in later period of comprehensive occupational health program than carly period without comprehensive program. The slope change in the analysis of linear regression of PbB on ZPP in lead workers can be used to evaluate the possible effect of health intervention to prevent undue high lead absorption in lead using industries. 6. 11 years occupational health service in this storage battery factory brought out considerable improvement of health status of lead workers in terms of decrease of PbB and ZPP and increase of Hb and the improvement was more remarkable after the introduction of comprehensive occupational health program.

상악동에 발생한 국균증 2례

조용석,김경원,박현진,강지연,이건국,신향미 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.1

국균증은 토양, 유기체 그리고 모든 형태의 유기물 잔사에 광범위하게 존재하는 국균 (Aspergillus) 속에 의하여 발병한다.이 균의 포자는 공기 중에서 전염될 수 있으므로 가장 흔한 감염 진균 중 하나이다.부비동에 발생한 국균증은 초기 임상 증상이 만성 부비동염과 유사하며, 방사선 사진 등을 이용한 검사상도 일반 세균성 감염과 구별하기가 매우 어렵다.대개는 병리조직학적 검사로 국균을 확인해서 확진을 내리게된다.국균증의 치료는 외과적으로 상악동내의 병소를 완전히 제거하고 비강에로 충분한 공기를 통기시키는 것이 가장 중요하며 보조적으로 전신적인 항진균제를 사용해야한다. 증례는 40세와 24세인 여자 환자들로서 전신적으로는 건강했으며 임상적 방사선적으로 만성 상악동 증세를 보였다.상악동 근치술을 시행하면서 국균종으로 여겨지는 암갈색 덩어리들이 관찰되었고 술 후 병리조직검사상 국균증으로 진단되었다.국균증으로 진단 후 보조적으로 전신적 항진균제를 투약하였으며 재발없이 치유되었다. Aspergillus is a fungus of the Acetomyces class that is most commonly encountered in the human environment.Aspergillosis of the maxillary sinus is not common and it requires special consideration to diagnose and eradicate. The disease is characterized by a wide range of initial symptoms, and should be considered as a possible diagnosis in sinusitis refractory to antibiotics and antral lavage. Generally the diagnosis of sinus aspergillosis can be confirmed by histologic examination, however, characteristic radiographic appearance such as coherene of radiodense sinus concretions can be found by careful examination of the radiograms. The choice of treatment of maxillary sinus aspergillosis is radical surgery with adjunctive systemic antifungal therapy. We experienced two cases of maxillary sinus aspergillosis, which initially diagnosed as chronic maxillary sinusitis and showed a necrotic mass intraoperatively and histologically diagnosed as aspergillosis.We reports theses cases with review of literature.

구조적 Network Viewer의 설계 및 구현

김용국,박수현,강석훈 東西大學校 1999 동서논문집 Vol.5 No.-

점차 방대해져가는 네트워크 환경하에서 기존의 네트워크 관리툴은 그 중요성을 더하고 있다. 그러나 기존의 네트워크 관리툴(NMS)은 너무 전문적인 정보만을 제공하기 때문에 일반 사용자가 사용하기 힘들다. 그래서 간단히 웹 브라우져상에서 일반적으로 많이 쓰이는 연결정보와 네트워크의 메타 정보만을 보여주는 플랫폼에 무관한 간이 네트워크 뷰어를 생각하게 되었고, COM 서버와 Active X 뷰어를 이용하여 간이 네트워크 뷰어를 만들게 되었다. 웹 브라우져 상이라면 운영체제에 상관없이 간단히 네트워크의 구성정보, 즉 메타 정보를 알수 있으며, 보안에 문제만 없다면 간단한 인증 절차로 공유도 가능하게 만들었다. 그리고, 화면 구성자체를 비주얼하게 만들어 초보자들도 쉽고 사용할 수 있게 만들었다.

골내 고정 장치용 초내식성 오스테나이트 및 듀플렉스 스테인리스강 가공재의 특성

오근택,강국진,박용수,김경남 大韓齒科器材學會 2003 대한치과재료학회지 Vol.30 No.3

Nickel is one of the most common causes of allergic contact dermatitis and hypersensitivity. In case of stainless steel as biomaterials, the higher the corrosion resistance and the lower the nickel content, the better it is or the human body. However, because nickel plays an essential role in the stabilization of the austenitic phase and the corrosion resistance of stainless steel, it is difficult to remove the nickel from the constituents of stainless steels completely. Therefore, the purpose of this study was to investigate corrosion resistance, cytotoxicity, and mechanical property of super stainless steels, which had high corrosion resistance by synergistic effect of Mo(or W) and N, with respect to nickel content and to evaluate their potential for orthodontic applications. Super austenitic stainless steel(SR-50A) had high nickel content of 22% and super duplex stainless steels nickel contents of 6~10%. Super austenitic and duplex stainless steels had nitrogen contents of above 0.2%. Also, these steels had high Mo or W contents which were able to increase the localized corrosion resistance. In terms of hardness, super stainless steels, which had Vickers hardness values of 374~400 Hv in cold-rolled state and 186~285 Hv in annealed state, exhibited higher values than 316L S. S.. Duplex stainless steels exhibited higher mechanical properties than austenitic stainless steels. In terms of corrosion resistance, super stainless steels exhibited lower passive current density(2.3~3.8 ㎂/cm^2) than pure Ti(6.6㎂/cm^2) in the body-simulated solution and similar potentiodynamic polarization behaviors. Pitting corrosion occurred in 316L S. S. but not in the others. Thus these steels were considered to have high corrosion resistance in the human body. In terms of cytotoxicity, SR-5OA and SAF2507 exhibited none cytotoxicity and the others slight. This results indicated that the cytotoxicity of alloys containing nickel had no relation with nickel content because SR-5OA with the highest nickel content exhibited none cytetoxicity In conclusion, it is considered that super stainless steels with high corrosion resistance and good biocompatibility can be used for orthodontic applications because of the enhanced passive film by high addition of Mo, W, N, etc.

산모혈과 제대혈에서 연농도에 관한 조사

이덕희,문종국,배강우,이용환,김진하,박강원 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.3

This study was conducted to evaluate the blood lead levels in 141 pregnant women who were not occupationally exposed to lead and their umbilical cord, and to identify some differences in blood lead levels by living area. The subjects were all delivered at St. Benedict Hospital in Pusan and the blood lead levels were measured by graphite furance atomic absorption spectrophotometer. The summarized results were as follows. 1. The geometric mean lead levels in maternal and cord blood were 8.0±1.8μg/dl, 5.0±0.6μg/dl respectively. 2. Lead levels in maternal(8.7±1.7μg/dl) and cord blood(5.4μ±0.7μg/dl) of industrial area(n=24) were higher than residential area(n=124) which were 7.9±1.7μg/dl and 5.0±0.6μg/dl respectively(p<0.05). 3. Maternal blood lead concentration was significantly correlated to cord blood lead concentration(r=0.6090, p<0.001), but other variables(maternal age, paternal smoking, birth weight, etc) were not correlated.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.