김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

항정신병약물을 처음으로 사용하는 초발 정신증 환자에서 나타나는 체중 및 대사성 지표의 변화 : 후향적 연구

김우진,심주철,공보금,강제욱,문정준,김정은,김민걸,박민경,김성진,김현정,정도운 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

Objectives:This study was to identify weight & metabolic changes in first-episode psychotic patients with antipsychotics use and investigate the differences of weight & metabolic changes between first-episode psychotic patients and controls with antipsychotics use. Method:In this retrospective study, twenty eight first-episode psychotic patients and twenty eight controls with schizophrenia, schizoaffective or bipolar disorder defined by DSM-IV criteria were included. Information about demographic and clinical characteristics of the subjects was gathered from the medical records. Also body weight, sBP, dBP, fasting glucose, lipid profile and WBC count were evaluated before and after antipsychotics use. Rogistic regression was conducted to assess factors associated with weight gain. Results:First-episode psychotic patients showed more weight and BMI changes than controls after antipsychotics use, and these changes continue over 12 months. On the other hand, there were no significant factors associated with weight gain. Conclusion:The results of present study suggest that antispychotics is one of the major causes inducing weight gain of psychotic patients and antipsychotics-induced weight gain is more vulnerable to drug-naive first-episode psychotic patients.

내시경적 점막절제술로 치험한 식도 과립상 세포종 1례

강혁주,김성욱,최석진,이중현,장재식,서영범,윤병구,박건욱,김성자,김용섭,강승완,이구,양창헌,이창우,김욱년,이광헌,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

과립상 세포종은 Schwann 세포 기원으로 생각되며 인체에 비교적 드물게 발생한다. 과립상 세포종은 전신 어느 곳에서나 발견될 수 있으나 주로 혀, 구강, 피부 혹은 유방 등에서 호발하며 드물게 위장관에서 발견된다. 위장관에서는 식도에서 가장 호발하며 다음으로 위, 대장 순이다. 과립상 세포종은 대부분, 특히 위장관에서는 양성이며 소수의 악성 병변이 보고되었다. 이러한 이유와 함께 수술 전의 진단이 어렵기 때문에 과립상 세포종에 대한 근본적인 치료는 현재까지 외과적 절제술이다. 최근에 시도되는 치료방법들로는 내시경적 레이저 치료, 용종절제술, 내시경적 점막 절제술 등이 있다. 저자들은 상부 소화관 내시경검사를 시행하여 식도 과립상 세포종을 진단하고 내시경적 점막 절제술을 시행하여 합병증 없이 퇴원하여 현재 재발없이 경과 관찰중인 1례를 경험하였기에 보고하는 바이다. Granular cell tumors, which occur infrequently, are probably of Schwann cell origin. They can occur almost anywhere in the body but usually affect the tongue, oral cavity, skin, or breasts and are rarely found in the gastrointestinal tracts. The esophagus is the most frequent gastrointestinal site, followed by the stomach and the colon. Granular cell tumors are generally benign, especially in the gastrointestinal tract, some malignant lesions have been reported. For this reason, and also because preoperative diagnosis is difficult, the standard treatment for granular cell tumor has until now been surgical excision. In recent years, other therapeutic methods is endoscopic laser therapy (ELT), polypectiomy, endoscopic mucosal resection (EMR). We report a case of esophageal granular cell tumor which was diagnosed by an endoscopy and managed using an endoscopic mucosal resection without complication.

경사진 트레드밀에서 후방보행운동을 통한 넙다리네갈래근 강화와 요통의 관계

박주완,김경,김형욱,김재우,배솔희,서정표,이정구,정하영 대구대학교 특수교육재활과학연구소 2010 再活科學硏究 Vol.28 No.1

목적: 본 연구에서는 경사진 트레드밀에서의 후방 보행 훈련을 통한 넙다리네갈래근의 강화가 요통 환자의 치료에 미치는 영향을 알아보고자 하였다. 대상: 만성 요통을 호소하는 16명의 환자를 대상으로 연구를 실시 하였다. 방법: 트레드밀을 10° 경사로 맞춰놓은 상태에서 대상자들은 하루 20분, 일주일에 3회의 빈도로 2주 동안 훈련을 하였다. 표면 근전도를 가지고 넓다리 곧은근, 안쪽 넓은근, 가쪽 넓은근, 척추 세움근의 %MVIC를 측정하였고, KODI와 VAS를 통해 대상자들의 통증정도를 측정하였다. 결과: 넓다리 곧은근은 훈련전에 비해 훈련을 하고 난 후 상대적으로 높은 근전도를 보였고, 통계적으로 유의하였다(p<.05). 안쪽 넓은근과 가쪽 넓은근 또한 높은 근전도율을 보였지만 통계적으로 유의하지는 않았다. 척추 세움근은 훈련전에 비해 훈련을 하고 난 후 높은 근전도율을 보여주었고, 통계적으로 유의하였다(p<.05). VAS와 KODI의 평균값은 명백하게 감소되었고, 통계적으로 유의하였다(p<.05). 결론: 이상의 결론을 종합하면, 후방보행을 통한 넙다리네갈래근의 강화는 요통의 감소에 효과적이라고 사료되며, 더불어 넙다리네갈래근으로 접근하여 요통을 경감하는 중재방법이 효과적인 치료 방법이 될 수 있다는 것을 의미한다. Purpose: This study was to investigate the influence of strengthening the Quadriceps femoris through backward walking treadmill training in the patients who have lower back pain. Subjects: Sixteen subjects with chronic lower back pain were included. Method: In the training, the treadmill was set to have a inclination of 10 degrees, and the subjects were trained for 20 minutes a day, 3 times a week, for 2 weeks. %MVIC(muscle power) of rectus femoris, vastus medialis oblique, vastus lateralis and erctor spinae were measured with surface EMG, and KODI and VAS were used to measure the subject's level of pain. Results: Quadriceps femoris showed relatively higher average electromyogram rates than that before training(p<.05). Vastus medialis oblique and vastus lateralis also showed higher average electromyogram rates, but no significant change in them was noted. Erector spinae showed higher average electromyogram rates than that before training(p<.05). The average points of VAS and KODI clearly decreased(p<.05). Conclusion: Strengthening Quadriceps femoris seems to be efficient for relieving lower back pain in this study.

정용 봄베를 이용한 단일 영역 연소 모델에 관한 연구

왕우경,서정주 여수대학교 1998 論文集 Vol.13 No.2

Combustion experiments were performed with fuel oils and injection rates in a constant volume combustion bomb simplified influences of combustion. Empirical coefficients of equations based on single zone model have been obtained by analysis of experimental data. The calculation results of single zone model showed similar trends comparing with the experimental data. But, it is necessary to examine exactly that the chemical characteristics of fuel oils have an effect of the beginning of the burning period. This combustion simulation program and empirical coefficients can be applied usefully to predict the combustion characteristics.

흰쥐 뇌세서의 Cholecystokinin mRNA 함유신경세포의 분포

김우태,배명애,이호섭,박매자,배용철,홍해숙,조희중,주강 慶北大學校 醫科大學 1994 慶北醫大誌 Vol.35 No.4

목적 : 뇌에서 Cholecystokinin(CCK)-8 함유신경세포의 분포에 대해서는 면역조직화학적기법을 이용하여 행한 연구가 많으나 CCK mRNA함유신경세포의 분포에 대한 연구는 아직 단편적이므로 본 연구에서는 이들 신경세포의 분포를 정확히 밝히고져 하였다. 대상 및 방법 : 흰쥐 뇌에서 ^35S가 표지된 oligonucleotide를 이용한 in situ hybridization기법으로 관찰하였다. 결과 및 결론 : 종뇌; 후구의 전후핵, 신피질, 이상엽, 후내야, 외측, 기저 및 피질 편도핵, 해마체, 그리고 선조체에서 CCK mRNA함유신경세포가 관찰되었다. 간뇌; CCK mRNA함유신경세포가 시상의 경우 전내측시상핵, 전복측시상핵, 외측시상핵, 내측시상핵, nucleus reunions, nucleus rhomboideus, 그리고 내측슬상핵에서 관찰되었으며 시상하부의 경우는 시삭상핵, 실방핵, 등내측시상하부핵, 그리고 유두상핵에서 관찰되었다. 중뇌; 흑질의 밀집부, 복측피개야, nucleus linea rostralis, 중심회색질, Edinger-Westphal nucleus, 그리고 하구에서 CCK mRNA 함유신경세포가 관찰되었다. 교 및 연수; CCK mRNA 함유신경세포들이 lateral parabrachial nucleus 삼차신경척수로고유핵, 거대그물구성체핵, 연수그물구성체, 그리고 삼차신경척수로핵에서 관찰되었다. 이상의 결과를 지금까 보고된 CCK-8함유신경세포의 분포에 대한 면역조직화학적 연구결과와 비교 하였을 때 대부분의 부위에서 CCK-8 및 CCK mRNA 함유신경세포의 분포가 유사하였으나 시상의 경우 CCK-8 함유신경세포는 검출되지 않은 반면 매우 많은 수의 CCK mRNA 함유신경세포가 존재함을 알 수 있었고 대뇌 피질의 경우 CCK mRNA 함유신경세포수가 CCK-8 함유신경세포보다 월등히 더 많았다. 반면, 등측봉선핵 및 고속핵에서는 CCK-8 함유신경세포가 다수 존재함이 보고되어 있으나 본 연구의 경우 CCK mRNA 함유신경세포를 관찰할 수 없었다. In order to investigate the distribution of cholecystokinin(CCK) mRNA containing neurons in the rat brain, brains were extirpated rapidly, frozen sectioned and processed for in situ hybridization technique with ^35S labeled oligonucleotide probe. The results obtained were as follows Telencephalon; CCK mRNA containing neurons were found in the anterior olfactory nucleus, neo-cortex, piriform cortex, entsorhinal cortex, lateral, basal and cortical amygdaloid nucleus, hippocampus and corpus striatum. Diencephalon; In the anterior medial thalamic nucleus, anterior ventral thalamic nucleus, lateral thalamic nucleus, medial thalamic nucleus, nucleus reuniens, nucleus rhomboideus, and medial geniculate nucleus, CCK mRNA containing neurons were found. In the hypothalamus, CCK mRNA containing neurons were located in the supraoptic nucleus, paraventricular nucleus, dorsomedial nucleus and supramammillary nucleus. Midbrain; CCK mRNA containing neurons were found in the substantia nigra pars compacta, ventral tegmental area, nucleus linea rostralis, substantia gricea centralis, Edinger-Westphal nucleus and inferior colliculus. Pons and Medulla oblongata; CCK mRNA containing neurons were found in the lateral parabrachial nucleus, nueteus originalis nervi trigemini, nucleus reticularis gigantocellularis, nucleus reticularis medullae oblongatae, nucleus tractus spinalis nervi trigemini.

유착성 관절낭염 치료에 있어서 한방 치료와 양방 치료의 임상적 고찰 : 동서협진 모델 개발을 위한 기초 연구를 중심으로

남동우,정인태,김주희,박유선,임사비나,이두익,이재동,이윤호,최도영 경희대학교 동서의학연구소 2006 東西醫學硏究所 論文集 Vol.2006 No.-

Objectives : To observe the effect of acupuncture treatment and western medical treatment on frozen shoulder patients. Methods : 39 voluntary patients were randomly assigned to the Eastern treatment group(E group, n=22) and the Western treatment group(W group, n=17). The E group received acupuncture treatment on LI15, TE14, GB21 and Master Dong's acupuncture points, Shin-gwan and Gyun-joong, twice a week for 4 weeks. The W group received suprascapular nerve block, subacromial injection and trigger point injection, twice a week for 4 weeks. Both groups were instructed to practice self exercise during their daily lives. Evaluations were made before treatment and after treatment using Constant Shoulder Assessment(CSA), Shoulder Pain and Disability Index(SPADI) and the patient's satisfaction concerning the treatment was measured by Visual Analogue Scale(VAS). The obtained data were analyzed and compared. Results : The E group showed significant improvement(p<0.05) according to the CSA and SPADI. The patient's satisfaction scored 5.67 on a scale of 10. The W group showed significant improvement(p<0.05) according to the CSA and SPADI. The patient's satisfaction scored 7.73. But the difference between the two groups were insignificant according to CSA and SPADI. Conclusion : Both acupuncture and nerve block treatment significantly improved frozen shoulder. But the difference of the two treatments was insignificant.

Efavirenz, indinavir, lopinavir, ritonavir의 LC-MS/MS를 이용한 동시 정량법

채정우,배경진,백인환,서정원,이병요,이은주,남진경,강원구,권광일 충남대학교 약학대학 의약품개발연구소 2009 藥學論文集 Vol.24 No.-

Efavirenz indinavir and kaleta (co-formulation of lopinavir and ritonavir) are important antiretroviral drugs which have been proved to be human immunodeficiency virus (HIV) protease inhibitors and reduced the morbidity and mortality associated with HIV-1 infection. A brief and fast high performance liquid chromatography coupled with electrospray mass spectrometry (LC-MS/MS, API 4000) method for the determination of 4 anti-retroviral agents (efavirenz, lopinavir, indinavir, ritonavir) in human plasma was developed and validated. A simple protein precipitation method was used on 100μl of human plasma. And internal standard solution (10 ng/ml methaqualone) 1ml and reconstitution solution (MeOH) 1ml were added. After vortexing for 30 s and centrifuging at 13,200rpm for 10min, 2μl of supernatant was injected into the column (XTerra MS C_(18) column, 2.1mm × 50mm 3.5㎛ particle size). The mobile phase consisted of MeOH and 0.1% formic acid in water (80:20 , v/v). The chromatogram was run for 1.5 min at a flow rate of 300μl/min. A triple quadrupole mass spectrometer was operated in a positive ion mode (lopinavir, indinavir, ritonavir) and negative mode (efavirenz), simultaneously and multiple reaction monitoring (MRM) was used for drug quantification. The precursor-to-product ion transitions of m/z 316→69 (efavirenz) and 629→447 (lopinavir) and 614→421 (indinavir) and 721→296 (ritonavir)were used to measure and quantify the analyte. The limit of quantitation (LOQ) was 50 ng/ml (efavirenz, indinavir, ritonavir) and 100 ng/ml (lopinavir). The weighted (l/y²) calibration curve was linear over human plasma range 50∼5000ng/ml (efavirenz), 100∼20000ng/ml (lopinavir), 50∼10000ng/ml (indinavir), 50∼2000ng/ml (ritonavir), correlation coefficient(r²) of 4 antiretroviral agents were higher than 0.998. Accuracies and intra-run precisions ranged within 86.60 and 113.29%, 1.06 and 11.16% for all 4 drugs analysed. This analytical method used to determine these drugs was fast and easy to perform, with minimal sample preparation, and without compromising precision and accuracy. The developed method was successful to determine antiretroviral agents in human plasma, and proved suitable for clinical pharmacokinetic study.