가면극과 인형극의 대비연구

김연숙 서강어문학회 1988 西江語文 Vol.6 No.1

「 삼국가시 」소재 설화 연구 : 「 온달(溫達)」,「 도미처(都彌妻)」, 「 설씨녀(薛氏女)」 설화에 나타난 ' 열 ' 사상

김연숙 서강어문학회 1994 西江語文 Vol.10 No.1

5-플루오로우라실에 의한 사람 구강암세포주의 단백질 발현 변화에 대한 면역침전 고성능 액체크로마토그래피 분석

김연숙 대한구강악안면병리학회 2017 대한구강악안면병리학회지 Vol.41 No.6

5-fluorouracil (5-FU) is a pyrimidine analog which can work as antineoplastic antimetabolite by blocking thymidylate synthetase conversion of deoxyuridylic acid to thymidylic acid in DNA synthesis. This study is aimed to know the anticancer effect of 5-FU on the expressions of important signaling proteins in KB cells through immunoprecipitation high performance liquid chromatography (IP-HPLC). KB cells were treated with 5 µM 5-FU and cultured for 12, 24, 48, 72, and 96 hours, and followed by IP-HPLC analysis using 32 antisera. 5-FU suppressed the proliferation of KB cells by decreases in the expressions of proliferation-related proteins, Ki-67, PCNA, CDK4, and MPM2 to 82.6%, 92.4%, 95.2%, and 95.9%, respectively, but increases of antiproliferation-related proteins, p16 and p21 to 106.7% and 125.5%, respectively, during 96 hours of experiment. This proliferation reduction was also negatively regulated by cMyc/MAX/MAD network signaling. The cellular protection and survival were consistently arrested by 5-FU treatment in KB cells. The expressions of NFkB, MDR, p-mTOR, and TNFα were decreased to 95.1%, 92.8%, 93.4%, and 90.3% in 48-72 hours, respectively, while cellular stress was increased by upregulation of p38 to 111.3% in 48 hours. And the expressions of pAKT1/2/3, hTERT, and AMPK were also decreased to 93.3%, 97.4%, and 89.3% in 24-48 hours, respectively, while the cellular transformation might be undergone by upregulation of TGF-β1 to 117% until 96 hours. Particularly, 5-FU treatment greatly induced the cellular apoptosis in KB cells by increased expressions of PARP, cPARP, caspase 9, c-caspase 9, caspase 8, and caspase 3 in the lack of p53/BAX and FASL/FAS signaling. The expressions of PARP and c-PARP were increased maximum to 119.2% in 24 hours, and followed by increases of caspase 9, c-caspase 9, caspase 8, and caspase 3 to 111.2%, 125.9%, 108.6%, and 116.3% in 72-96 hours. Therefore, it is presumed that 5-FU induced cellular apoptosis in KB cells may be derived from the overexpression of PARP due to the increased DNA defect caused by 5-FU, which can lead to ATP depletion and subsequent cellular apoptosis.

Immunoprecipitation-Based High Performance Liquid Chromatography Analysis of Human Mixed Saliva

김연숙 대한구강악안면병리학회 2009 대한구강악안면병리학회지 Vol.33 No.6

As the human mixed saliva plays important roles for the protection, regeneration, immunity, and molecular transfer/signaling in the oral and gastro-intestinal mucosa, the salivary contents have great implications for the general health of human body. Nevertheless, the analysis method of human saliva has not been well developed up to date, because the proteins of mixed saliva are rapidly interacted with each other and easily degraded by proteolytic enzymes and microorganisms. This study aims to develop an immunoprecipitation-based high performance liquid chromatography (IP-HPLC) for the analysis of human mixed saliva. The representative IP-HPLC analyses were performed to compare among different subjects in variable general conditions. Compared to the normal control the subjects suffered from bacterial infections of gastro-intestinal enteritis, chronic periodontitis, and acute necrotizing gingivo-stomatitis showed dramatic increase of LL-37 level depending on the severity of diseases, while the subject suffered from Herpes stomatitis, a viral infection showed great increase of β-defensin 2. These data indicate that LL-37 in human mixed saliva is more responsible to the bacterial infections of gastro-intestinal enteritis, chronic periodontitis, and acute necrotizing gingivo-stomatitis, while β-defensin 2 is more responsible to the viral infection of Herpes stomatitis. This study also suggeststhat the IP-HPLC be easily applicable to the wide range of biological samples for the quantitative analysis of an objective protein.

면역침전 고수행 액체 크로마토그래피를 이용한 타액분석 방법의 개발

김연숙 대한구강악안면병리학회 2009 대한구강악안면병리학회지 Vol.33 No.6

As the human mixed saliva plays important roles for the protection, regeneration, immunity, and molecular transfer/ signaling in the oral and gastro-intestinal mucosa, the salivary contents have great implications for the general health of human body. Nevertheless, the analysis method of human saliva has not been well developed up to date, because the proteins of mixed saliva are rapidly interacted with each other and easily degraded by proteolytic enzymes and microorganisms. This study aims to develop an immunoprecipitation-based high performance liquid chromatography (IP-HPLC) for the analysis of human mixed saliva. The representative IP-HPLC analyses were performed to compare among different subjects in variable general conditions. Compared to the normal control the subjects suffered from bacterial infections of gastro-intestinal enteritis, chronic periodontitis, and acute necrotizing gingivo-stomatitis showed dramatic increase of LL-37 level depending on the severity of diseases, while the subject suffered from Herpes stomatitis, a viral infection showed great increase of β-defensin 2. These data indicate that LL-37 in human mixed saliva is more responsible to the bacterial infections of gastro-intestinal enteritis, chronic periodontitis, and acute necrotizing gingivo-stomatitis, while β-defensin 2 is more responsible to the viral infection of Herpes stomatitis. This study also suggeststhat the IP-HPLC be easily applicable to the wide range of biological samples for the quantitative analysis of an objective protein.

인공피부모델을 이용한 피부자극시험

김연숙,이수현,류양환,정행선 한국동물실험대체법학회 2010 한국동물실험대체법학회 학술대회집 Vol.2010 No.1

‘도덕․윤리 교육의 모학문으로서 철학(윤리학)과 도덕․윤리 교과교육학의 편제’에 대한 토론

김연숙 한국윤리교육학회 2008 한국윤리교육학회 학술대회 Vol.2008 No.1

IP-HPLC Analysis of Human Salivary Protein Complexes

김연숙,이석근 대한구강악안면병리학회 2015 대한구강악안면병리학회지 Vol.39 No.5

In order to know the characteristic roles of salivary protein complex (SPC) the gel-filtration chromatography was performed using the unstimulated and the stimulated whole saliva separately. The first and second dominant SPC peaks were fractionated and analyzed by immunoprecipitation HPLC (IP-HPLC) using antibodies against the essential salivary proteins including α-amylase, mucin-1, proline rich proteins (PRPs), histatin, cystatin, LL-37, lysozyme, lactoferrin, -defensin-1, -2, -3, IgA, transglutaminase 4 (TGase 4), mucocidin, α1-antitrypsin, cathepsin G. In the gel-filtration chromatography the stimulated whole saliva showed much reduced amount of SPCs than the unstimulated whole saliva, but the proportional patterns of both whole saliva were almost similar each other. Through IP-HPLC analysis both of the first and second dominant SPCs were variably positive for the essential salivary proteins, however, α-amylase, mucin-1, PRPs, lysozyme, and cathepsin G were predominant in the first dominant SPC, while cystatin, lactoferrin, β-defensin-1, -2, -3, IgA, mucocidin, TGase 4, and α1-antitrypsin were predominant in the second dominant SPC. And more, the α1-antitrypsin and cathepsin G which were mostly derived from gingival crevicular fluid were also consistently found in the SPCs. These data may suggest that the first dominant SPC, rich in α-amylase, mucin-1, PRPs, lysozyme, and cathepsin G, may play a role in food digestion, protein degradation, and mucosa lubrication, while the second dominant SPC, rich in cystatin, lactoferrin, β-defensin-1, -2, -3, mucocidin, IgA, TGase 4, and α1-antitrypsin, may play a role in the mucosa protection and antimicrobial defense.

High Performance Liquid Chromatography Analysis of Human Salivary Protein Complexes

김연숙,이석근 대한구강악안면병리학회 2014 대한구강악안면병리학회지 Vol.38 No.6

Salivary proteins include numerous functional proteins which play important roles not only for the food-intake but also for theprotective and defensive mechanisms. In the present study the compositions of salivary proteins were analyzed by different methods,including electrophoresis and high performance liquid chromatography (HPLC). In hydrophobic protein HPLC analysis the parotid salivagradually produced macromolecular complexes when agitated in refrigerator until 30 minutes. These salivary protein complexes weredigested by neuraminidase, and then migrated more rapidly in native tris glycine gel than the control. Therefore, it was assumed thatthe glycosylated proteins of parotid saliva became gradually aggregated to form salivary protein complexes similar to those of wholesaliva. The salivary protein complexes were easily degenerated in different experimental buffers, i.e., SDS buffer, tris glycine buffer,methanol, etc., and resulted non-specific patterns in electrophoresis and HPLC. Therefore, it was presumed that the salivary proteincomplexes was made by the hydrophobic interaction as well as electrostatic attraction between salivary proteins. These data indicatedthat to know the real pattern of salivary protein complexes in vivo the whole saliva should be analyzed by HPLC using non-adheringcolumn with isoelectric buffer. Consequently, the whole saliva was analyzed by HPLC using reverse phase SuperoseTM column with20 mM potassium phosphate buffer, and two prominent peaks of salivary protein complexes were consistently found in every people. These salivary protein complex peaks were relatively stable up to 6 hours after saliva collection when the whole saliva was keptin refrigerator during experiment. Therefore, it is suggested that the salivary protein complex patterns are characteristic macromolecularstructures of whole saliva, which are also applicable as a diagnostic point in different saliva-associated diseases.

항암화학요법중 호중구 감소증이 발생한 저위험군 발열 환자에서 경구 ciprofloxacin 요법의 임상적 유용성

김연숙,김춘관,김신우,김성민,백경란,김원석,윤성수,강원기,이홍기,박근칠,박찬형,송재훈 대한내과학회 1999 대한내과학회 추계학술대회 Vol.57 No.-