마우스에서 Selenium 이 Natural Killer Cell 활성능에 미치는 영향

최지선,표명윤 숙명여자대학교 약학연구소 2003 약학논문집-숙명여자대학교 Vol.19 No.-

Effect of Selenium (Se) on natural killer (NK) cell activity of spleen in mice was investigated. Se lppm or 3ppm was orally administered to ICR mice in drinking water for 18 days. Cyclophosphamide (CY) was injected i.p. to mice with a single dose of 10 mg/kg b.w. on the last day of Se-administration. Spleen cells were obtained 24h after CY-injection and used as effector cells. NK cell cytotoxicity (%) was assessed in a 4h ^(51)Cr-release assay against YAC-1 target cells. The treatment of Se alone significantly increased NK cell cytotoxicity at low dose (lppm), but decreased at high dose (3ppm) when compared with control group. The NK cell activity (LU_(30)) was significantly enhanced in Se (lppm) plus CY, but inhibited in Se (3ppm) plus CY when compared with the treatment of CY alone.

석류 과피 추출물의 라디칼 소거활성

최윤정,오영지,정동선 서울여자대학교 2010 자연과학연구논문집 Vol.22 No.-

Antioxidant activity and total phenolic contents of Punica granatum peels were investigated. The pomegranate peels were extracted with 70% methanol (ME) and then partitioned to hexane (HF), ethyl acetate (EAF) and water (WF) fractions. Total phenolic contents and radical scavenging activities of each extract and fraction from Punica granatum peels were measured. The ethyl acetate fraction showed the highest radical scavenging activity, followed by methanol extract, water fraction and hexane fraction. The IC50 values of EAF were 0.144 ㎍./mL and 0.50 ㎍./mL for DPPH and ABTS radicals scavenging activities, respectively. A strong antioxidant activity of the EAF from pomegranate peels was correlated with its high level of total phenolic contents (740.0 mg of tannic acid equivalent/g). ME and WF also exhibited relatively strong radical scavenging activities with IC50 values of 6.70 ㎍./mL and 7.25 ㎍./mL for DPPH radical, and 17.4 ㎍./mL and 18.33 ㎍./mL for ABTS radical, respectively. These results suggest that the pomegranate peels possess strong antioxidant activity, and can be a useful source of natural antioxidant.

Selenium 이 마우스 혈중 마크로파지의 탄소입자 탐식능에 미치는 영향

최지선,표명윤 숙명여자대학교 약학연구소 2004 약학논문집-숙명여자대학교 Vol.20 No.-

Effects of Selenium (Se) on the carbon clearance activity of macrophage in blood were investigated in mice. Se was orally administered in drinking water for 18 days (1ppm or 3ppm) or intraperitoneally with a single dose of 0.05, 0.25, 0.5mg'kg b.w. to normal and CY-treated ICR mice. Cyclophosphamide (CY) was injected i.p. to mice with a single dose of 20 mg/kg b.w. on the last day of Se-administration. Carbon suspension was injected i.v. at 0.01ml/g b.w. in mice 48hrs after the administration of experimental chemicals. Blood was collected from retro-orbital venous plexus of mice 5min, 10min after injection of carbon suspension and carbon clearance (t_(1/2), min) was assessed. The treatment of Se alone slightly increased carbon clearance activity of circulating macrophage when compared with control group. Carbon clearance activity was significantly enhanced in Se plus CY when compared with the treatment of CY alone. These results indicate that Se seemed to significantly restore the reduction of phagocytic activity induced by CY.

유두상 에크린 한선종 1예

최현성,이지현,윤석권,김한욱,임철완 대한피부과학회 2004 大韓皮膚科學會誌 Vol.42 No.3

Papillary eccrine adenoma is a rare benign sweat gland neoplasm. It occurs most often as a solitary nodule in women and is located predominantly on the distal portion of the extremities. This tumor is similar to tubular apocrine adenoma in histological and clinical aspects, so distinction of these tumors is still controversial. We report a case of two papillary eccrine adenomas developed on the left popliteal and calf area of a 58-year-old-man, this is the first report of the multiple papillary eccrine adenomas in Korea. (Korean J Dermatol 2004;42(3):353~355)

예비수학교사의 신념에 따른 교수학적 내용지식(PCK)과의 관련성에 관한 연구

고상숙,김은호,문정윤,배지은,정대진 이화여자대학교 교과교육연구소 2011 교과교육학연구 Vol.15 No.4

This study investigated the relationship between PCK (Pedagogical Content Knowledge) and pre-service mathematics teachers' mathematical beliefs, and the effects of PCK on their teaching. The data were collected through pre & post interviews, a questionnaire, and classroom observations of six pre-service mathematics teachers who were taking their field practica in their senior year. The results indicate that the pre-service mathematics teachers' differing beliefs about the nature of mathematics affected their PCK. This study showed the relationship between their beliefs about teaching and the learning of mathematics and PCK. It is recommended that more opportunities for PCK development, as well as for modifications in of their beliefs have to be offered in education programs for pre-service mathematics teachers. 본 연구의 목적은 예비수학교사들의 수학적 신념과 수업에서 발현된 교수학적 내용지식(PCK)의 특징을 알아보고, 이를 통해 수학적 신념에 따른 PCK의 관련성에 대해 분석하는 것이다. 이 연구는 교육실습 중인 예비수학교사 6명을 대상으로 실시한 PCK 설문지, 인터뷰 자료, 수업관찰 자료를 분석하는 질적 연구 방법을 토대로 하였다. 연구결과, 예비수학교사들의 다양한 수학 본질에 대한 신념은 PCK에 서로 다른 영향을 주었으며, 수학 교수-학습에 대한 신념과 PCK사이에는 밀접한 관련성이 있음을 볼 수 있었다. 따라서 신념과 교수학적 내용지식(PCK)을 함께 발달시킬 수 있는 예비교사 연수 및 지원 프로그램의 확충이 필요하다.

Selenium이 마우스의 일차 체액성 면역반응과 접촉성 과민반응에 미치는 영향

최지선,표명윤 숙명여자대학교 약학연구소 2005 약학논문집-숙명여자대학교 Vol.22 No.-

Effects of Selenium (Se) on the primary humoral immune response and contact-delayed type hypersensitivity (CDTH) to 2,4-dinitro-l-fluorobenzene (DNFB) in normal and cyclophosphamide (CY)-treated mice. Se was orally administered via drinking water for 18 days (1ppm or 3ppm) or via intraperitoneal (i.p.) injection with a single dose of 0.05, 0.25, 0.5 mg/kg b.w. to normal and CY-treated mice. CY was i.p, injected to mice with a single dose of 10 or 50 mg/kg b.w. on the last day of Se-administration. When only Se was administered simultaneously with or after immunization, splenic IgM plaque forming cells (PFC) and hemmaglutinin-titer (HA-titer) in mice were not changed, however, decreased in the mice which were administered with high dose of Se before immunization. In addition, they were significantly increased in the concomitant treatment of CY and Se, compared to them in the only CY-treatment. Se significantly decreased DNFB-induced CDTH and inhibited the CY-enhanced CDTH. These results indicate that Se may inhibit the immunotoxicity induced by CY.

표피낭종에 발생한 전염성 연속종 1예

문지훈,문용주,최혜자,윤석권 대한피부과학회 2003 대한피부과학회지 Vol.41 No.6

We present an unusual case of molluscum contagiosum occurring in epidermal cyst on back of a 5-year-old girl. Histology showed an intradermal cyst containing lamellated horny materials and typical molluscum cclls. In Korean literature, 5 such case were reported. We report an additional case of molluscum occurring in epidermal cyst. (Korean J Dermatol 3003;41(6) : 790~792)

조혈모세포이식 후 발생한 주폐포자층 폐렴에 대한 고찰

주지현,최정현,이동건,백지연,고윤호,이혜정,김세희,신호진,박윤희,박지영,김유진,신완식,김춘추 대한감염학회 2001 감염 Vol.33 No.4

Background : Pneumocytitis cainii pneumonia (PCP) can occur in immunocompromised hosts especially such as AIDS or cancer patients. Although recent research had focused on PCP in AIDS patients, few studies have described the clinical presentations of PCP in recipients of stem cell transplantation (SCT). We evaluated the clinical manifestations of PCP in SCT patients admitted at St. Mary's hospital, Seoul, Korea. Methods : The medical records of 17 PCP patients undergoing SCT between Feb. 1998 and Feb. 2000 were reviewed. The diagnosis of PCP was confirmed through the demonstration of Pneumocytitis cainii via either cytology of brochoalveolar lavage (BAL) or histological technique of lung biopsy. CMV disease and CMV infection were confirmed by BAL culture and antigenemia respectively . Results : Seventeen patients were all recipients of allogeneic SCT and 7 of 17 patients were performed non-sibling SCT. Patients presented with symptoms including brief period (4 ∼23 days) of fever (76%), dyspnea (70%), cough (64%), and signs such as rare(58.8%), Sixteen patients (94%) had been receiving immunosuppressive agent such as cyclosporine A (64%) or Fk506 (35%) without PCP prophylaxis. Eleven patients (64%) were treated with corticosteroid with mean dose of 16 mg/day prednisolone and mean duration of 4.6 months after post-SCT period. Twelve patients were co-infected with CMV. Another co-infected miCroorganisms were Pseudomonas aeruginosa, Mycobacterium tuberculosis, herpes simplex virus, parainfluenza virus, Average duration of treatment with trimethoprim-sulfamethoxazole (TMP/SMX) was 21 ±9 days. Four patients died, and three of them were related with PCP. Conclusion : PCP developed frequently in patients who were taking immunosuppressive drug due to graft versus host disease or were not taking TMP/SMX prophylaxis. High risk patients showing fever, cough, or dyspnea should be considered to take early bronchoscopic intervention for detection of PCP. When treat for PCP, it also be considered to the possibility of coinfection such as CMV. (Korean J Infect Dis 33:273∼279, 2001)

사회적 상호작용을 강조한 과학 탐구실험의 효과

김지영,성숙경,박종윤,최병순 한국과학교육학회 2002 한국과학교육학회지 Vol.22 No.4

사회적 상호작용을 강조한 과학탐구실험이 학생들의 학업성취와 과학탐구능력 및 학습동기에 미치는 효과에 대해 연구하였으며, 탐구실험 수업이 소집단의 구성방식에 따라 어떠한 차이를 나타내는지 알아보기 위해 인지수준이 동질인 집단과 이질인 집단의 효과를 비교하였다. 이를 위해 중학교 2학년 255명을 통제집단과 실험집단으로 나누어, 실험집단에는 사회적 상호작용을 강조한 과학탐구실험 수업을, 통제집단에는 전통적인 실험 수업을 1년간 적용한 후 과학학업성취도, 과학탐구능력, 학습동기 검사를 실시하였다. 사회적 상호작용을 강조한 탐구실험은 이끌어내기, 친숙해지기, 구성하기, 확장하기의 4단계로 구성되어 있으며, 관찰 현상을 학습과 연관시키고 실험을 설계하며 결론을 이끌어내는 과정을 통해 동료 및 교사와 상호작용을 할 수 있도록 구성되어 있다. 연구 결과 사회적 상호작용을 강조한 과학탐구실험은 학생들의 학업성취와 과학탐구능력 및 학습동기에서 유의미한 효과가 있었다. 모든 인지수준의 학생들에게 효과가 나타난 것은 아니었으나 대분분의 인지 수준에서 학업성취의 효과가 크게 나타났으며, 학업성취에 비해 과학탐구능력의 향상정도가 낮았다. 소집단 구성에 따른 효과에서는 동질집단이 이질집단에 비해 성취도와 학습동기에서 유의미한 향상이 있었다. 이러한 결과는 과학탐구실험에서 상호작용이 중요하며, 상호작용이 학생들의 성취도와 탐구능력 향상에 긍정적 영향이 있음을 시사한다. This study investigated the effects of scientific inquiry experiments emphasizing social interaction on the academic achievement, the ability for science inquiry and the learning motivation of the students. To examine the differences among the classes of scientific inquiry experiments according to the way of organizing small groups, the effects of the group in homogeneous cognitive level and the group in heterogeneous cognitive level were compared. 255 7th-graders were grouped into the treatment group and the control group. After the treatment group received the scientific inquiry experimental lessons emphasizing social interaction and the control group received traditional lessons for one year, academic achievement, ability for scientific inquiry, and learning motivation were examined. These inquiry experiments were composed of four steps which are eliciting, familiarizing, constructing and bridging. And students can interact with peers and teachers through the process of relating observed phenomena to concept, constructing experiment procedure, and eliciting results. The ANCOVA results revealed that there were significant effects of science inquiry experiments emphasizing social interaction on the academic achievement, the ability for scientific inquiry and the learning motivation of the students. The significant academic achievement of the students in most of the cognitive levels, if not all, was found and degree of improvement in the ability for scientific inquiry was lower than that in the academic achieve3ment. Concerning the effects according to the way of organizing small groups, there were significant improvement in academic achievement and learning motivation of the group in homogeneous cognitive level compared to the group in heterogeneous cognitive level. These results indicate that social interaction in learning is important and it has positive influences on the improvement in academic achievement and ability for scientific inquiry of students.

쌓기 활동에서 유아들의 친 사회적 행동 출현에 대한 맥락적 이해

박화윤,마지순,최재숙 한국영유아보육학회 2002 한국영유아보육학 Vol.0 No.31

The purpose of this ethnographic study is to explore the emergent context of prosocial behavior and to find the children's character promoting of prosocial that within the area of block. The result are as follows: 1) It appeared that emergent context of prosocial; behavior is completion of structures, participation of symbolic play using them and clean-up. 2) It appeared that children's character promoting prosocial behavior that appearence of altruism by children's perspective-taking, exchage of idea and problem-solving.