Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Adult Patients with Congenital Muscular Torticollis Treated with Bipolar Release : Report of 31 Cases

Lee, Gun Sang,Lee, Myung Ki,Kim, Woo Jae,Kim, Ho Sang,Kim, Jeong Ho,Kim, Yun-Suk The Korean Neurosurgical Society 2017 Journal of Korean neurosurgical society Vol.60 No.1

Objective : We assessed the surgical results of bipolar release in 31 adult patients with uncorrected congenital muscular torticollis (CMT) and more than 12 months of follow-up. Methods : Thirty-one patients underwent a bipolar release of the sternocleidomastoid muscle (SCM) and were retrospectively analyzed. The mean follow-up period was 14.9 months (range, 12-30). The mean age at time of surgery was 30.3 years (range, 20-54). Patients were evaluated with a modified Lee's scoring system, cervicomandibular angle (CMA) measurement, and a global satisfaction rating scale using patient self-reporting. Results : The modified Lee's scoring system indicated excellent results in 4 (12.9%) patients, good in 18 (58.1%), and fair in 9 (29.0%) at the last follow-up after surgery. The improvements in neck movement and head tilt were statistically significant (p<0.05). The preoperative mean CMA was $15.4^{\circ}$ (range, 5.4-29.0), which was reduced to a mean of CMA of $6.3^{\circ}$ (range, 0-25) after surgery (p<0.05). The global satisfaction rating scale was 93.7% (range, 90-100). A transient sensory deficit on the ipsilateral lower ear lobe was noted in three cases. No significant permanent complications occurred. Conclusion : Bipolar release of the SCM is a safe and reliable technique for the treatment of CMT in adults.

개에서 결직장 연접부의 림프종 1례

이재일,이수진,김영석,김명진,조성환,신상태,이영원,김명철 충남대학교 수의과대학 동물의과학연구소 2003 動物醫科學硏究誌 Vol.11 No.-

A five years old female Shih-tzu was referred to the veterinary medical teaching hospital at Chungnam national university with soft bloody diarrhea and tenesmus. On rectal palpation, solid mass was palpated in colorectal region. In radiographs, small intestine was deviated cranially, descending colon and rectal junction were deviated caudoventrally in lateral projection of the abdomen. In ultrasonographs, hypoechoic nodulation of mesenteric lymph node in colorectal junction, mixed echoic thickening of colorectal wall and irregular margination were observed. Rectal endoscopy revealed that colorectal men was partially obstructed due to compression of the mass. Also, severe ulceration, inflammation and hemorrhage were also observed in descending colon. The mass was resected through the laparotomy. In histopathology, it was diagnosis of lymphoma in the colorectal junction.

토양환경지도 자료를 이용한 콩밭 토양의 화학성 비교

박지숙,이민진,정재원,김미혜,이서연,지윤미,한준호,김유학,공명석,한광현,노희명 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

It is important to interpret the soil chemical properties in order to effectively manage the farmland. This study was conducted to investigate the soil chemical properties of upland soybean field according to the soil series at 32 upland fields located in the parts of Gangwon-do. Soil sampling sites were selected by using the National Statistics DB and KSIS DB that is a soil environment information system provided by the Rural Development Administration. Soil samples were collected from 0~15 cm of top soil before seeding or transplanting, air-dried, passed through a 2 mm sieve, and analyzed for soil pH, EC, organic matter, available phosphorus (P2O5), exchangeable potassium, exchangeable calcium and exchangeable magnesium. The average chemical properties have exceeded the each nutrient optimum range level of RDA, except the organic matter in experimental soil 2015. And despite application of conventional fertilization, each soil series nutrient contents were change respectively. The results of this study suggest that conventional fertilization should be applied based on the analysis about the amount of accumulated nutrient contents in the soil.

Immobilization with Ketamine HCI and Tiletamine-Zolazepam in Cynomolgus Monkeys

Myung-cheol Kim,Sung-hyeok Hong,Soo-jin Lee,Young-suk Kim,Jae-il Lee 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

with Ketamine HCl and Tiletamine-Zolazepam in Cynomolgus MonkeysJae-il Lee, Sung-hyeok Hong, Soo-jin Lee, Young-suk Kim and Myung-cheol Kim*Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, KoreaReceived March 4, 2003 / Accepted July 2, 2003J. Vet. Sci. (2003), 4(2), 187-191JOURNAL OFVeterinaryScience*Corresponding author: Myung-cheol Kim Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea Tel: +82-42-821-6757, Fax: +82-42-822-4216 E-mail: mckim@cnu.ac.kr

ATHB12, an ABA-lnducible Homeodomain-Leucine Zipper (HD-Zip) Protein of Arabidopsis, Negatively Regulates the Growth of the Inflorescence Stem by Decreasing the Expression of a Gibberellin 20-Oxidase Gene

Ora Son,허윤선,김윤경,이현정,김성한,김미란,남경희,이명석,김부용,박종범,박정안,이석찬,Atsushi Hanada,Shinjiro Yamaguchi,이인정,김승기,윤대진,Eva So¨derman,천충일 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

ATHB12, an ABA-lnducible Homeodomain-Leucine Zipper (HD-Zip) Protein of Arabidopsis, Negatively Regulates the Growth of the Inflorescence Stem by Decreasing the Expression of a Gibberellin 20-Oxidase Gene

Ora Son,허윤선,김윤경,이현정,김성한,김미란,남경희,이명석,김부용,박종범,박정안,이석찬,Atsushi Hanada,Shinjiro Yamaguchi,이인정,김승기,윤대진,Eva So¨derman,천충일 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

내분비계 교란물질의 검출계를 이용한 γ-HCH의 미생물에 의한 중간대사산물에 대한 내분비계교란 활성의 평가

이행석,박주석,조은민,문명숙,太田明德,류재천 대한상하수도학회 2003 상하수도학회지 Vol.17 No.1

To develop an efficient degradation system for Endocrine disruptors (EDs), it is necessary to have a good system to evaluate rapidly and accurately endocrine-disrupting activities of suspected chemicals and their degradation products. We previously constructed a co-expression system of GAL4 DNA binding domain (DBD)-human estrogen receptorβ ligand binding domain (hERβ LBD) and Gal4p transcriptional activation domain (TAD)-co-activator SRC1 in Saccharomyces cerevisiae with a chromosome-integrated lacZ reporter gene under the control of CYC1 promoter and GAL4 binding site (Upstream Activating Sequence, UAS). Expression of this reporter gene was dependent on the presence of estrogen or endocrine disruptors in the culture medium. Furthermore, the extent of transcriptional activation by those chemicals correlated with their estrogenic activities measured by other assay systems, indicating that this assay system is efficient and reliable for measuring estrogenic activity. We applied this assay system to measure estrogenic activity of microbial degradation products of γ-hexachlorocyclohexane (γ-HCH) by Sphingomonas paucimobilis. Among the γ-HCH metabolites, 2,5-dichlorohydroquinone (2,5-DCHQ) and chlorohydroquinone (CHQ) had similar estrogenic activities to the original chemical, but hydroquinone (HQ), a metabolite at later stage, had no activity at the concentration of 10^-4M, showing the necessity of evaluation of intermediate metabolites in microbial degradation systems.

개항기 소설과 야담에 나타난 서구 인식

이윤석,정명기 洌上古典硏究會 2003 열상고전연구 Vol.17 No.-

이 연구는 개항이 시작된 19세기 중반부터 서양의 활판인쇄술을 받아들여 활판본으로 고소설과 야담집이 출판되기 시작하는 1910년대까지로 시기를 한정해서, 이 시기에 읽힌 서사물에 나타나는 서구에 대한 인식을 보는 것이다. 이 연구에서 분석의 대상으로 삼는 서사물은, 소설은 고소설과 신소설이고, 야담은 개항기 이전에 이미 알려진 야담과 개항기에 나온 단형서사물이다. 고소설과 전통 야담에는 서구에 대한 내용이 없으므로, 이들이 1910년대에 활판본으로 출간되면서 일어난 변이 가운데 서구에 대한 인식이 들어 있는 것을 찾아보았다. 그러나 이들 활판본 고소설과 야담은 전대의 것을 그대로 옮겨놓은 것이므로 야담과 고소설에서는 서구에 대한 인식을 찾아볼 수 없었다. 개항이라는 사회변화가 전통 서사물의 독자들에게는 아무런 영향을 미치지 못했다는 것을 알 수 있었다. 개항기의 단형 서사물과 신소설에는 서구에 관한 내용이 많이 나타나지만, 이들 서구에 관한 내용은 매우 피상적일뿐 아니라, 이야기의 진행에 필수적인 것이 아니다. 서구에 대한 내용이 자주 나타나는 것이 서구에 대해 긍정적인 시각을 드러내는 것 같지만, 자세히 살펴보면, 서구에 대한 언급은 통속적인 이야기의 흥미를 더하기 위한 보조 역할만을 한다. 개항기를 해명하는 데 있어서 이 시기에 읽힌 서사물은 중요한 자료가 될 것이다. 개항기 이전부터 서구에 대한 비판적 시각을 갖고 있는 한시는 말할 것도 없고 개항기의 시가에는 서구에 대한 비판적 인식이 일찍부터 제기되었는데, 왜 서시물에서는 서구에 대한 비판적 인식이 잘 나타나지 않는가 하는 문제도 앞으로 관심을 갖고 연구해야할 과제라고 생각한다. This research is about the perception of The West in Korean narrative literature from 1860's when Korea began to open its door to the West till 1910's when old novel and Yadam(野談) started to be published in movable type printing. The Narrative literature we were focusing on includes old novel(古小說) and new novel(新小說) in novel part and traditional Yadam and editorial(敍事的新聞論說) published during this period. As there is no clear mention on The West in old novel and traditional Yadam, we attempted to find some perception of The West in movable type printing in 1901's were only the copies of previous version, we could not find any of the sort. It seems that social changes had no influence upon traditional narrative literature readers in those days. We found that in editorial and new novel contain many details about The West. However, those details were not only superficial, but also not vital for threads of the stories. Details of The West were mentioned frequently in those days in affirmative manner, but as we looked through carefully on this subject we found that mentions on The West only played the role of increasing readers' interest.

산마늘로부터 단리한 kaempferol과 quercetin의 콜레스테롤 저하 활성

이성숙,문서현,이학주,최돈하,조명행 한국목재공학회 2004 목재공학 Vol.32 No.1

식용 임산자원인 산채류를 기능성 식품으로 개발하고자 산마늘을 비롯한 충 13종의 에탄을 조추출물에 대한 콜레스테롤 저하활성을 검정하였다. 즉, 콜레스테롤 생합성 과정 초기에 관여하는 3-hydroxy-3-methylglutaryl coenzyme A reductase(HMG-CoA reductase)와 후기에 관여하는 squalene synthase의 효소 활성을 조사한 결과 산마늘 잎 에탄올 추출물이 두 효소의 활성을 공히 70% 이상 저해하여 활성이 가장 우수한 것으로 나타났다. 그리고 이러한 콜레스테롤 저하활성과 관련이 있는 물질을 탐색하고자 산마늘로부터 물질 단리를 시도하여 디클로로메탄 가용부로부터 kaempferol과 quercetin을 단리하였다. 또한 유전자 레벨에서의 콜레스테롤 저하 활성을 조사하기 위해 단리물질과 분획물을 C100세포(햄스터 유래 HMG-CoA reductase 고발현 세포주)에 각각 5㎍/㎖과 10㎍/㎖로 24시간 처리하여 HMG-CoA reductase와 squalene synthase의 mRNA 발현 정도를 조사하였다. 그 결과 10㎍/㎖로 kaempferol과 quercetin을 처리한 경우 두 효소의 mRNA가 전혀 발현하지 않는 것으로 나타나 유전자 레벨에서의 콜레스테롤 생합성 저해 효과를 확인할 수 있었다. 이상의 결과 산마늘 잎 에탄올 추출물은 콜레스테롤 생합성에 관여하는 HMG-CoA reductase와 squalene synthase의 활성을 저해하며 이러한 활성 저해 효과는 kaempferol과 quercetin에 기인하는 것으로 사료되었다. 특히 kaempferol과 auercetin은 여러식물의 성분으로서 이미 알려진 화합물이지만 콜레스테롤 저하활성이 있는 것으로 밝혀진 것은 이번이 처음으로 금후 이들 물질과 이들 물질을 함유하고 있는 식물 활용에 필요한 자료를 제공하였다고 사료된다.