Choline dehydrogenase interacts with SQSTM1/p62 to recruit LC3 and stimulate mitophagy.

Park, Sungwoo,Choi, Seon-Guk,Yoo, Seung-Min,Son, Jin H,Jung, Yong-Keun Landes Bioscience 2014 AUTOPHAGY Vol.10 No.11

<P>CHDH (choline dehydrogenase) is an enzyme catalyzing the dehydrogenation of choline to betaine aldehyde in mitochondria. Apart from this well-known activity, we report here a pivotal role of CHDH in mitophagy. Knockdown of CHDH expression impairs CCCP-induced mitophagy and PARK2/parkin-mediated clearance of mitochondria in mammalian cells, including HeLa cells and SN4741 dopaminergic neuronal cells. Conversely, overexpression of CHDH accelerates PARK2-mediated mitophagy. CHDH is found on both the outer and inner membranes of mitochondria in resting cells. Interestingly, upon induction of mitophagy, CHDH accumulates on the outer membrane in a mitochondrial potential-dependent manner. We found that CHDH is not a substrate of PARK2 but interacts with SQSTM1 independently of PARK2 to recruit SQSTM1 into depolarized mitochondria. The FB1 domain of CHDH is exposed to the cytosol and is required for the interaction with SQSTM1, and overexpression of the FB1 domain only in cytosol reduces CCCP-induced mitochondrial degradation via competitive interaction with SQSTM1. In addition, CHDH, but not the CHDH FB1 deletion mutant, forms a ternary protein complex with SQSTM1 and MAP1LC3 (LC3), leading to loading of LC3 onto the damaged mitochondria via SQSTM1. Further, CHDH is crucial to the mitophagy induced by MPP+ in SN4741 cells. Overall, our results suggest that CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition.</P>

지리산국립공원 심원계곡 생태경관 복원공사지역 식생 변화

정태준 ( Tae-jun Jung ),김연경 ( Yeon-Gyeong Kim ),김영진 ( Young-jin Kim ),정명희 ( Myung-hee Jung ),박경희 ( Kyoung-hee Park ),신창근 ( Chang-keun Shin ),박선홍 ( Seung-hong Park ),김영선 ( Young-sun Kim ) 한국환경생태학회 2021 한국환경생태학회지 Vol.35 No.3

본 연구는 지리산국립공원 심원계곡 생태경관 복원공사지역의 모니터링 결과를 분석하여 체계적인 복원관리를 도모할 수 있는 기초자료를 확보하는데 그 목적이 있다. 복원사업이 완료된 2017년도에 식생 모니터링을 위한 조사구 12개소와 대조구 4개소를 설치하였고, 2017년과 2020년의 상대우점치, 종다양도지수, 유사도 등의 변화를 분석하였다. 복원사업 시 수목이 식재되었던 조사구는 종다양도지수가 0∼1.4552로 나타났고, 대조구와의 유사도지수에서는 1개 조사구가 1.32%로 분석된 것을 제외하고 모두 0%로 나타났다. 조사구의 종다양도지수 및 유사도지수가 매우 낮은 것은 복원사업 시 식재된 수목이 고사하거나 멧돼지 피해, 유수에 의한 침식으로 유실되는 등의 영향으로 판단되었다. 반면 복원사업 시 수목이 식재되지 않은 미식재 조사구는 종다양도지수가 0.9538∼2.3222로 나타났고, 유사도지수는 최대 8.33%까지 분석되었다. 국립공원 생태경관 복원공사지역의 복원 공법 개발을 위한 장기 모니터링과, 미식재 조사구의 천이과정을 분석하는 지속적인 연구가 필요하다. This study aims to obtain basic data for systematic restoration by analyzing the monitoring results of the Shimwon Valley Ecological Landscape Restoration Project area in Jirisan National Park. In 2017, when the restoration project was completed, 12 monitoring plots and 4 control plots were installed for vegetation monitoring, and changes in the relative dominance, species diversity index and similarity between 2017 and 2020 were analyzed. The species diversity index of the surveyed areas where trees were planted during the restoration project was 0-1.4552, and the similarity index with the control group was 0% except for one survey area at 1.32%. The very low species diversity index and similarity index in the survey areas were attributed to the loss of trees planted during the restoration project due to death, damage by wild boars, or erosion by running water. On the other hand, the species diversity index was 0.9538-2.3222 in the monitoring plot where no tree was planted, and the similarity index was analyzed to be as high as 8.33%. It is necessary to continue the long-term monitoring for the development of ecological landscape restoration methods in the national park and analysis of the succession in monitoring plots where no trees were planted.

김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

당 첨가량에 따른 송이정과의 물성 및 관능 특성

박미란 ( Mi Lan Park ),최수근 ( Soo Keun Choi ),정인창 ( In Chang Jung ),변광인 ( Gwang In Byun ) 한국식생활문화학회 2006 韓國食生活文化學會誌 Vol.21 No.6

최종사용자중심의 DSS실행 성공요인에 관한 연구

박정근 京畿大學校 韓國産業經濟硏究所 1994 産業硏究 Vol.5 No.-

육상 운동 지식이 초등학생의 정의적 특성에 미치는 영향 : Focused on a long distance race in physical education for 6 grade students in elementary school

박근호,전중기 대구대학교 인문과학 예술문화연구소 2003 人文科學硏究 Vol.25 No.-

The purpose of this paper is to make a comparative study of educational effects that teachers' knowledge obtained by studying text has on physical education. This will analyze how a teacher with special knowledge from text reaches study aim in physical education, and influences students who intend to accomplish study subject. The comparative study was conducted between two types of teachers. One accumulates propositional knowledge and practical knowledge of physical education according to study aim, the other does according to their experience. The focus of this study is a long run race, an athletic sport in physical education for 6 grade students in elementary school. The study result is dealt by SPSS(7.52) and shows as below. First, the teacher who instructed students after studying text developed psychomotor domain, The experimental group consists of people who study text and instruct athlete sports. While the control group depends on its experience. The research result shows an educational effect of experimental group is more significant than the control group, regardless of sex. Second, the teacher who instructed after studying text changed affective domain positively. Third, teacher's professionalism gained through studying text made higher effects on psychomotor domain than affective domain. Forth, increasing interrelation among subordinate factors of psychomotor domain was show in the experimental group, compared to the control group

다형성 교모세포종에서 광범위 전적출술후 단기 재발된 치험 1예

박호권,강중식,조준,문창택,장상근,임정애 건국대학교 의과학연구소 2003 건국의과학학술지 Vol.13 No.-

Glioblasty/ma Multiforme(GBM) is the one of the most common malignant tumors in central nervous system(CNS). The incidence of the tumor is about 12% of all CNS tumors and about 50% of all intracranial tumors. Though the development of the microsurgical technique, radiosurgical technique, and chemotherapy works effectively, complete cure of the GBM has not been achieved. It has been known that the prognosis of GBM depends on the extent of surgical tumor removal, remained tumor volume, radiation dose, and chemotherapy. Not only patient's age but also tumor characteristics such as MRI enhancement, angiogenesis and necrosis are also very important prognostic factors. Authors experienced very aggressive GBM in aged woman, who received gross total tumor removal, but refused other therapeutic motalities postoperatively. Very rapid recurrence of GBM and global cerebral edema in aged woman should be considered fatal. This case demonstrates the effectiveness of aggressive removal and the limited usefulness of neurosurgical tools in GBM recurrence. Authors would suggest that the early diagnostic effort and the verified neurosurgical therapy for malignant tumor are still strongly requested to make better prognosis.

실신을 주소로 응급의학센터에 내원한 환자에 대한 임상적 연구

박정배,김상은,배성만,정호성,박철완,이 근 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Syncope is not uncommon problem that needs broad differential diagnosis. Correct diagnosis of the cause of syncope is important, because although some causes of syncope such as vasodepressor syncope have benign prognosis, others such as ventricular tachycardia may need prompt diagnosis and treatment. We present a retrospective study of the patients with episodes of syncope brought to Emergency Medical Center of Chung Ang Gil Hospital from January 1994 to October 1994 and the results were as followings. 1. The patients with episodes of syncope accounte for 0.5% of total Emergency Department visits. 2. The male to female ratio was 4 : 6 and mean age was about 54-years old. 3. The cause of syncope was identifiable in 48% of the case and not in 52%. The noncardiac cause(56%) was more common than the cardiac(44%) and cardiac, noncardiac, SUO(Syncope of Unknown Origin) ratio was 1 : 1.4 : 2.6. 4. 29% of patients were dignosed by history taking, physical examination, and electrocardiography when they visited the Emergency Department initially, and 88% of patients with identifiable causes were diagnosed through initial Emergency Department investigation and Holter monitioring. 5. The rate of correct diagnosis can be increased if initial investigation includes Holter monitoring, and the further research for more accurate, inexpensive and noninvasive diagnostic method for syncope is needed.

前腕部 經穴 取穴에서 骨度分寸法과 一夫法의 比較 硏究

박히준,채윤병,차웅석,박종배,이혜정,이향숙,인창식,고형균,김수영,최일환,김강식,문정배,배기태,유경환,육근영,정병주,손인철,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

Background : The cun measurement System, an essential and convenient method in locating acupoints, has been widely used in the practice of acupuncture. However, traditional cun measurement has been criticized for its lack of reliability. Objectives : The purposes of this study are to determine if one cun measured by the directional methods have a consistency with that of proportional methods and to investigate which factors are related with these differences, especially in forearm. Methods : The distance between the elbow crease and the wrist crease of forearm was compared to a reference value of one cun obtained by the directional method. In this method, one cun is one third of the distance between index finger and Small finger of a subject, measured at proximal interphalangeal joint. In addition, to investigate the factors influencing the differences between these two methods, we measured the height and body weight and calculated body mass index (BMI). Finally we analyzed the factors correlated with these lengths by linear regression test. Results : The results showed that one cun obtained by the directional methods were significantly different from one cun by the proportional methods in forearm. It was demonstrated that the length acquired with the directional method was more correlated with body weight and body mass index, while the length obtained by the proportional method was more correlated with the height.

일부 부인과 양성 질환자의 지방조직 및 혈청의 PCBs와 DDE 농도

박성균,이강숙,노영만,구정완,민선영,한진구,고원경,김미란,정재근,이종승,김진홍 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.1

목적 : 본 연구는 지방조직과 혈청의 PCBs와 DDE 농도를 측정하고, 이에 영향을 미치는 것으로 알려진 요인들과의 관련성을 평가하고자 하였다. 방법 : 유방암, 자궁내막증 등 유기염소계 화합물의 영향이 있는 것으로 알려진 호르몬 관련 질병을 제외한 부인과 환자 52명을 대상으로 지방조직과 혈청을 분석하였으며, 설문조사를 통하여 연령, 교육수준, 직업, 비만도, 흡연과 음주, 출산력, 수유력등을 조사하였다. 지방조직과 혈청의 PCBs와 DDE 농도는 가스크로마토그래피를 사용하여 측정하였다. 결과 : 지방조직의 경우, 13개 PCBs congeners 농도의 합은 중앙값이 48.29 ng/g, 기하평균값이 56.78 ng/g이었고, DDE 농도는 중앙값이 142.89ng/g, 기하평균값이 117.06 ng/g이었으며, 혈청의 경우는 PCBs congeners 농도의 합은 중앙값이 4.67 ㎍/L, 기하평균값이 4.85 ㎍/L이었고, DDE 농도는 중앙값이 1.75 ㎍/L, 기하평균값이 2.09 ㎍/L로 나타났다. 지방조직과 혈청의 DDE 농도 사이는 높은 상관성을 보였으나(r²=0.310, p=0,0002), PCBs는 상관성이 약한 것으로 나타났다(r²=0.029, p=0.2582). 영향요인을 범주화한 후 로그변환 시킨 지방조직과 혈청의 PCBs, DDE 평균의 차이를 분석한 결과, 지방조직의 DDE만이 출산자녀수(p=0.015), 초산연령 (p=0.014), 비만지수(p=0.035)와 연관성이 발견되었다 다중회귀분석의 결과 지방조직의 DDE는 출산자녀수가 적을수록, 초산연령이 늦을수록 증가하는 경향을 보였다. 결론 : 출산자녀수와 초산연령, 비만도가 지방조직의 DDE와 관련성을 보였으며, 또한 유기염소계 화합물의 장기간의 노출에 의한 영향을 평가하는데 있어서 혈청보다 지방조직이 더 유용한 생물지표임을 알 수 있었다. Objectives : This study was purposed to determine the levels of PCBs and DDE in adipose tissue and serum and to evaluate the relations with factors affecting these levels. Methods : We analyzed adipose tissues and sera from 52 gynecologic benign disease patients aged 27-78 years, except hormonal diseases such as breast cancer and endometriosis. We also surveyed age, education, occupation, body mass index (BMI), smoking, drinking, number of children, and duration of breastfeeding by questionnaires. Tissue and serum levels of PCBs and DDE were measured using gas chromatography. Results : The median and geometric mean levels of PCBs were 48.29 ng/g, 56.78 ng/g for adipose tissue and 4.67 ㎍/L, 4.85 ㎍/L for serum, and those of DDE were 142.89 ng/g, 117.06 ng/g for adipose tissue and 1.75 ㎍/L, 2.09 ㎍/L for serum, respectively. Adipose tissue and serum levels for DDE showed high correlation (r²=0.310, p=0.0002), but those of PCBs didn't (r²=0.029, p=0.2582). In analyses of the differences of the means of log transformed adipose tissue and serum PCBs and DDE levels for groups of potential covariates, only adipose tissue DDE levels were significantly associated with number of children (p=0.015), age at first birth (p=0.074) and BMI (p=0.035). In multiple regression analysis, adipose tissue DDE levels were significantly increased with decreasing number of children and increasing age at first birth. Conclusions : This study suggested that parity and adiposity were associated with levels of DDE in adipose tissue, and adipose tissue is a better biomarker than serum for evaluating the long-term exposure of organochlorines.