연부조직 육종에서 Cyclin D_(1) 유전자의 증폭에 대한 연구

유재훈,정동준,이정은,김창진 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The molecular mechanisms of the soft tissue sarcoma is not elucidated. In general, deregulations of the several genes such as oncogenes, antioncogenes, genes regulating apoptosis and cell cycle are known to take part in the carcinogenesis of most carcinomas. Moreover, deregulation of the cell cycle is one of the major carcinogenic mechanisms. The cyclin D_(1) is the most important gene regulating the transition, G_(1). to S, which is the most importan check point in cell cycle. The cyclin D_(1) gene amplification was reported in various carcinomas, but not in sarcomas. In this study, we studied the cyclin D_(1) expression in 10 liposarcomas, 5 rhabdomyosarcomas and 5 malignant fibrous histiocytomas by imunohistochedmistry, and real-time polymerase chain reaction. The results were as follows; 1. Immunohistochemically, liposarcoma and malignant fibrous histiocytomas expressed moderate degree of cyclin D_(1), however expression was noted in few rhabdo- myosarcomas. 2. The cycline D_(1) gene amplification was not different between normal and sarcomas(p>0.05). 3. There was no positive correlation between cyclin D_(1) expression by immunohistochemistry and cylin D(1)gene amplification. Above results suggest that cyclin D_(1) gene amplifacation does not contribute to the oncogenesis of the sarcomas, and cyclin D_(1) expression by immunohistochemistry does not correlate with the gene amplification. As the sample cases are limited in this study, forther studies with more samples are needed.

최근 5년간 급성 A형 간염으로 입원한 환자들에 대한 입원 내용 분석

장재영,유병욱,조용진,오정은,홍성호,조주연 순천향대학교 의학연구소 2009 Journal of Soonchunhyang Medical Science Vol.15 No.1

Abstract Background : Due to improvements in Korea's sanitary conditions, antibody rates to the hepatitis A virus is decreasing rapidly and admission due to the disease is increasing, Patients who were admitted for hepatitis A during the last 5 years were selected and yearly differences in admission, clinical picture, medical fees, and differences according to age and gender were analyzed. Methods : Patients who were admitted to the Seoul Soonchunhyang University Hospital during the 5 year period between May of 2003 and April of 2008 and tested positive for IgM anti-HAV were selected and their medical records retrospectively analyzed. Results : 156 subjects [91 male (58%), 65 female (42%) were selected. The average age was 29.2 years (range; 21.4-37), average admission period 12.6 days (range; 12.5-1 7.7) days, and average medical fees 2,295,151 (range; 932,097-3,658,205) KRW(Korean Won), Yearly incidence rates were as follows : 10 for May, 2003 to April, 2004, 14 for May, 2004 to April, 2005, 23 for May, 2005 to April, 2006, 69 for May, 2006 to April, 2007, and 41 for May, 2007 to April, 2008, The maximum value median of AST, ALT, and bilirubin during admission was 989 IU/L, 1999 IU/L, and 5.6 mg/dL each, Each factors were all positively correlated with the patient's age. Conclusions : Recent decrease in antibody positivity of the hepatitis A virus and subsequent surge of acute hepatitis A is a cause of rising socioeconomic costs. A large scale epidemiologic research on whether HAV vaccination should be made mandatory and health insurance applied seems to be needed. Key words : Hepatitis A, Age, Medical fees, Vaccination

황사의 지속시간에 따른 대기 중 농도변동 및 종관기상장 특성

김유근,송상근,강재은 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.4

We determined Asian dust days by constructing the standard of Asian dust using PM_10 concentrations (≥150 ugm-³, 24-hr average) and TOMS aerosol index (≥0.7) for 5 years (1998-2002), and grouped into long-lasted cases (LLCs, ≥4 days) and short-lasted cases (SLCs, ≤2 days) concerning the mean lasting time (about 3 days) of Asian dust. Further we performed the specific analyses associated with concentration variations and synoptic conditions by using PM_10 and TOMS data, weather maps during the dusty cases (LLCs and SLCs). As a result, the LLCs (9 cases) had large variations of PM_10 concentration as the mean of 131 ugm-³ and the maximum mean of 379.8 ugm-³, and showed dominant features the continuous passage of deep trough caused by blocking effect and weak trough (56%, 5 cases) over Korea. The SLCs (11 cases) had relatively small variations of PM_10 concentration as the mean of 133.3 ugm-³ and the maximum mean of 247.2 ugm-³, and showed passage of one weak trough (64%, 7 cases) over Korea. Thereafter, the case studies (April 7-13, 2002 of LLC and March 23-24, 2000 of SLC) performed by the simulation of MM5 with meteorological variables of the horizontal wind, potential temperature, isentropic potential vorticity, and helped to better understand the features of synoptic conditions in connection with the concentration variations for each case.

운동이 정신분열증 환자의 세로토닌과 도파민 분비에 미치는 영향

박은경,김용권,유재현,진영수 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of exercise training on Serotonin and Dopamine level in schizophrenic patients. The subjects consisted of 12 schizophrenic patients and 6 normal individuals between 20 and 30 years old, were invided into three groups: 1) Experimental group(N=6), 2) Control group(N=6),3) Compared group(N=6). Subjects participated in aerobic and strength exercise program for 8 weeks according to the exercise prescriptions. They performed exercise for 1 hours per day, three times a week at 50-60% of HRpeak. The changes of serotonin, dopamine level in the subjetcs before and after the treadmill exercise program have been measured. The results were been compared to serotonin, dopamine between all groups. The results were as follows; 1. Effects of exercise training on serotonin level in three groups were not changed significantly and changed significantly within those groups after acute exercise and resting. Interaction effect within group and treatment time was not significantly changed. 2. Effects of exercise training on dopamine level in three groups were not changed significantly and changed significantly within those groups after acute exercise and resting. Interaction effect within group and treatment time was not significantly changed.

혈당 측정에 있어서 반사광 비색법과 전기 화학 전극법의 비교

황유성,이도훈,우재근 충북대학교 의과대학 충북대학교 의학연구소 1995 忠北醫大學術誌 Vol.5 No.1

당뇨병환자에서 적절한 혈당을 유지하기 위한 목적으로 시험지봉 검사 방법이 1970년대 초 처음으로 개발된 이후 많은 자가 혈당기들이 고안되어 사용되고 있으며, 대부분이 반사광 비색법을 이용한 자가 혈당 측정기로 현재 국내에는 Glucometer(Ames), Glucoscan(Eiken), One TouchTRM Ⅱ(Lifescan), Accu-check Ⅲ(BM)등이 사용되고 있다. 저자들은 본원에서 이미 사용하고 있었던 반사광 비색법을 이용한 시험지봉으로 LIFESCAN사의 One TouchTM Ⅱ와 전기 화학 전극법을 이용한 Medisense사의 CompanionTM 2에 대하여 정밀도, 직선성, 비교방법과의 상관관계 및 혈액량과 헤마토크리트에 의한 영향, 관찰자간의 변이, 항응고제가 미치는 영향 등 측정에 영향을 미치는 여러 인자들에 대하여 평가를 시행하였다. 검사일내 정밀성(within day precision)은 두 기기 모두 5% 정도이었고, 검사일간 정밀성(day-to-day precision)은 CompanionTM 2가 3.14%∼6.53%, One touchTM Ⅱ가 4.69%∼8.13%의 변이계수를 보였다. 두 기기 모두 직선성이나 비교방법과의 상관관계가 우수하였다. CompanionTM 2와 One touchTM Ⅱ사이의 상관관계는 y=1.040x+21.679, r=0.9716이었다. 두 기기 모두 혈액량, 관찰자, 항응고제에 의해서는 영향을 받지 않았으며, 헤마토크리트에 의해서는 헤마토크리트가 낮을 때는 높은 혈당치를, 헤마토크리트가 높을 때는 낮은 혈당치를 나타냈다. The purpose of this study is to assess two monitors of blood glucose : One touch?? Ⅱ(Lifescan Inc.), which is using reagent strips by reflectance photometry and CompanionTM 2(Medisense Inc.), which is using electrochemical electrode method. Glucose was determined on capillary and venous blood samples from 70 adult diabetics by three different methodologies : One touchTM Ⅱ, CompanionTM 2 portable glucose meters (capillary) and the Hitachi 7150 analyzer(venous), which is using hexokinase method. In within-run precision studies for One touchTM Ⅱ and CompanionTM 2, coefficient variations (CVs, %) were 1.92∼5.09, 2.95∼4.79 at three different levels, respectively. In between-day percision studies for One touchTM Ⅱ and CompanionTM 2, CVs(%) were 4.69∼8.13, 3.14∼6.53 at three different levels, respectively. The linearity studies revealed r=0.9996 in One touch?? Ⅱ, r=0.9977 in CompanionTM 2, which were satisfactory. Correlation equation and coefficient for results by One touchTM Ⅱ and CompanionTM 2 vs those by hexokinase method(x) were : y=0.8087x-8.9566(r=0.9824) for One touchTM Ⅱ, y=0.8885x+4.4992(r=0.9807) for CompanionTM 2, which were satisfactory. The glucose levels were falsely high at low hematocrit concentration and falsely low at high hematocrit concentration in both monitors. The volume of blood applied to the test strips is not critical. We concluded that "One touchTM Ⅱ" using reagent strip by reflectance photometry and "Companion?? 2" using electrochemical electrode method is suitable for self monitoring of blood glucose.

느타리버섯의 갈반병 원인균에 대한 길항세균 선발에 관한 연구

조재선,이은관,유승오,전억한 경희대학교 생명자원과학연구원 1997 硏究論文集 Vol.18 No.-

갈반병이 이병된 느타리 버섯과 버섯 재배장 주변 토양으로부터 채집한 균주로부터 갈반병 원인 균주 4개와 갈반병 길항 균주 3개를 선발 하였다. 선발된 균주를 API 20E kit에 의해 동정한 결과 갈반병 원인 균주 P-24, P-28은 P. putida로 P-27, P-33은 lane 1: PCR marker lane 2: P.fluorescens P. tolaasii로 동정 되었으며, 길항 균주중 A-11, A-20은 P. fluorescence로 A-29는 Pseudomonas sp.로 동정 되었으나, RAPD PCR 방법에 의한 유전적 유사성은 모든 균주가 다른 양상을 보였다(Fig. 5). 이는 생화학적 방법에 의한 Pseudomonas 계통의 균주 동정의 기술이 확립되지 않은 점도 있으며, 유전적 유사성은 달라도 같은 속의 균주가 많은 것으로 사료 된다. 길항성은 갈반병 원인 균주 대 길항 균주가 1:1의 농도로 길항성이 양호하여 Nair등(1972)의 보고 보다 우수한 길항성을 보였다. Screening experiments were carried out in order to select bacteria causing brown blotch disease on the mushroom. especially, Pleurotus ostreatus. Four brown blotch disease causing bacteria were isolated from the Pleurotus ostreatus and the soil around mushroom farm. Three other strains, A-11, A-20, A-29 showing antagonism against four brown blotch causing bacteria through pitting test and cross checking and found to be identified as Pseudomonas fluorescence for A-11 and A-20, and Pseudomonas sp. for A-29, respectively. Colony morphology test also showed that A-11 and A-29 were appeared as transparent gel with green color and A-20 colony showed opaque gel with light green color.

국내 상급종합병원과 종합병원 간호사의 정맥주입간호실무지침의 확산정도

은영(Young Eun),구미옥(Mee Ok Gu),조용애(Young Ae Cho),정재심(Jae Shim Jeong),권정순(Jeong Soon Kwon),유정숙(Cheong Suk Yoo5),정영선(Young Sun Jeong),정인숙(In Sook Jung),김경숙(Kyeong Sug Kim),이선희(Seon Heui Lee),서현주(Hyun Ju S 한국근거기반간호학회 2015 근거와 간호 Vol.3 No.1

Purpose: This study was conducted to investigate the extent of diffusion of Intravenous infusion nursing practice guideline among nurses in advanced general hospitals and general hospitals in Korea. Methods: The subjects were 234 nurses who practice the intravenous infusion in 24 advanced general hospitals and general hospitals. Data were collected between October 5 and November 2, 2015 by mail (return rates: 97.5 %). Data were analyzed using descriptive statistics, t-test, and ANOVA with SPSS/ WIN 21. Results: The average guideline diffusion score and levels of diffusion was 3.39±0.58 (level of “use sometimes”). 44 recommendations (46%) were in level of “use always” and 50 recommendations (53%) were in “use sometimes”. Extent of diffusion were significantly different according to present status (F=2.81, p=.040) and education (F=4.35, p=.014). The facilitating factors to use the guideline were education by department of nursing service, convenient composition of guideline and barrier factors were “no time to use the guideline”, “don’t know the guideline” and “there is no guideline at ward”. Conclusion: Extent of diffusion of Intravenous infusion nursing practice guideline among nurses was moderate level. The strategies for promoting the implementation of guideline in clinical practice is needed. Further study of outcome evaluation of guideline implementation will be needed.

Acriflavine-Guanosine 복합제 (AG60)의 조직내 분포에 대한 형광현미경적 연구

고정식,임수재,안의태,김진국,김상건,이경영,강종구,홍은경,정영신,유보림,한영복 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

선형유도전자펌프내의 자기유체 유동에 관한 수치적 연구

최훈기,유근종,박태봉,은재정,장남영 국립7개대학공동논문집간행위원회 2003 공업기술연구 Vol.3 No.-

Numerical analysis is performed for magnetic and MHD flow fields in Electro-Magnetic(EM) pump. A finite volume method is applied to solve magnetic field governing equations and the Navier-Stokes equations. Vector and scalar potential methods are adopted to obtain the electric and magnetic fields and the resulting Lorentz force in solving Maxwell equations. The magnetic field and velocity distributions are found to be influenced by the magnitude of the Reynolds number and the phase of applied electric current. Computational results indicate that the magnetic flux distribution with changing phase of electric current is characterized by a pair of counter-rotating closed loops. The velocity distribution of the flow field is influenced on the intensity of Lorentz force.

Calcofluor White 염색법을 이용한 주폐포자충 폐렴의 조기 진단

고윤석,최정은,송재훈,김유겸,김원동,조영주,유빈,김양수,주용선 대한감염학회 1993 감염 Vol.25 No.2

To evaluate the clinical efficacy and usefulness of Calcofluor white stain in the diagnosis of Pneumocystis carinii pneumonia (PCP), we performed Calcofluor white stain in bronchoalveloar lavage fluid from 10 compromised patients with fever , dyspnea, and diffuse pulmonary infiltrates. Four of 10 cases showed positive staining for P.carinii with Calcofluor white staining. Underlying diseases were ALL, AML, AIDS, and Pinealoblastom, respectively. These patients had been treated with Bactrim for 2 or 3 weeks with complete cure. Calcofluor white stain has clear advantages over conventional staining methods such ad Gomori methenamine silver, Toluidine blue O, or Giemsa stain in terms of speed and simplicity of the technique. This method is believed to be suitable for early diagnosis of PCP. Application of this rapid diagnostic method to suspected cases with febrile pneumonitis syndrome will increase the detection rate of PCP in Korea.