고압산소(高壓酸素)가 폐조직(肺組織)에 미치는 영향(影響)과 DL-α-Tocopherol의 내성효과(耐性效果)에 관(關)한 연구(硏究)

이원창,윤화중,Lee, Won Chang,Yoon, Wha Joong 대한수의학회 1975 大韓獸醫學會誌 Vol.15 No.1

Experimental studies were performed to observe the effect of exposure to 100% oxygen in 2 atmospheres on the lung tissue of rats, and to examine the resistant effect of DL-${\alpha}$-tocopherol. The following results were made through this experiment: 1. Half-lethal time by oxygen poisoning was longer in tocopherol treated group than not treated group. 2. Ratio of lung weight to body weight was significantly higher in fatal group within half-lethal time than survival group (p<0.01). 3. Histopathological changes of the lung by oxygen toxicity were vascular congestion, pulmonary edema, hemorrhage and emphysematous change. The degree of changes were rather marked in experimental group than tocopherol untreated group. Those were regard as the changes being occurred during tolerance process by prolonging half-lethal time.

The role of hLHX6-HMR as a methylation biomarker for early diagnosis of cervical cancer

정삼일,정동준,김진선,Lisha Yi,구근호,이재혁,이순덕,박진화,BOOGI CHANG,김창환,김창진,이명숙 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

The role of hLHX6-HMR as a methylation biomarker for early diagnosis of cervical cancer

정삼일,정동준,김진선,Lisha Yi,구근호,이재혁,이순덕,박진화,BOOGI CHANG,김창환,김창진,이명숙 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

톨루엔을 포함한 유기용제의 직업적 폭로로 인한 신경내분비계 영향

이채언,이종태,정의화,손혜숙,문덕환,전진호,강정학,이창희,김휘동,김종한,정귀옥 大韓産業醫學會 1995 대한직업환경의학회지 Vol.7 No.2

Long term occupational exposure to solvent mixtures may cause adverse effects to the central nervous system with neurobehavioral symptoms. And some organic slovents have been suggested to cause impairment of tuberoinfundibular dopaminergic activity and neurochemical mechanisms controlling pituitary secretion. For the purpose of assessing neuroendocrine effects in occupational solvents exposure, hormonal study settings (shoes-manufacturing industry & fishing products industry) and compared with nonexposed controls(33 men, 85 women). Male workers exposed to solvent mixtures had significantly lower plasma level of follicle-stimulating hormone(FSH) than nonexposed male controls. While female workers exposed to solvent mixtures had significantly lower plasma levels of growth hormone(GH), and thyroid-stimulating hormone(TSH) than nonexposed female controls. The results of significant decrease in plasma concentrations of pituitary hormones(FSH, GH, and TSH) in workers exposed solvent mixtures indicate that occupational exposure to solvent mixtures may induce neuroendocrine effects through an effect on hypothalamic pituitary axis.

Coproporphyrin 簡易 定量法의 改善에 關한 硏究

李昌和,宋東彬,車喆煥 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.1

Rimington (1971) developed a satisfactory method for quantitative determination of urinary coproporphyrin (CP), which is essentially needed for diagnosis of lead poisoning in suspected persons. But it is found to be unsuitable for a mass screening because it is Iaborious and complex in its procedures. Soulsby (1974) modified the method into a simple and convenient one by reducing the steps in extraction procedure. In spite of its simplicity and usefulness for screening purpose, aythor experienced that CP determination by the method was inconsistent and erroneous. Thus, it was not satisfactory and acceptable if accurate determination of CP is needed as in screening test for lead poisoning. The present study was performed to find a simple and accurate method that is applicable for the quantitative determination and mass screening by modifying Soulsby’s method. The results were as follows: 1. The amount of CP extracted with diethylether was shown to be highly dependent on urine pH, when extraction was performed one time instead of three times. The optimal pH was 5.4 where 98% of CP was recovered with one time extraction. 2. When the diethylether layer was washed with distilled water, it was possible to avoid interference in UV-spectrophotometry caused by contaminated organic materials. 3. With modification as above, average recovery rate of CP from urine was 99.5% with a coefficient of variance 2.1% on vaiidity test. 4. The results suggested that Soulsby’s method can be improved to a method with both simplicity and accuracy by simple modifications, i.e., one time extraction with adjusting the urine pH to 5.4 and washing the diethylether later containing CP with distilled water.

홍삼추출물 투여가 Paraquat 투여 흰쥐 체내 혈청철에 미치는 영향

이화재,장재철 群山大學校基礎科學硏究所 1998 基礎科學硏究 Vol.13 No.-

We investigated in the contents of TIBC, SI and UIBC in the mouse blood after treatment with paraquat and/or ginseng extracts. Blood TIBC were mild decreased less than 10% both of 24 hours and 96hours paraquat group than control group. And administration of lipophilic extracts were highest elevated effect on TIBC level. Lipophilic extracts were best increased(p<0.01) on SI level. But alcohol soluble extract were rapidly normalization stabled by crisis of paraquat on SI level. And total saponine were not appeared specific effect on blood TIBC, SI, and UIBC than other ginseng extract in rats. Water soluble extract were highest increase(p<0.01) on UIBC. And blood UIBC were increased p<0.05 in 24 hours paraquat group but after 96 hours late were decreased p<0.01. That the result of SI were very intensely reacted decrease until 50% SI(p<0.01) in initial 24 hours paraquat but 96 hours later were too much increased about 306%(p<0.01) on serum level than 24hours initial paraquat.

대장균군 검사용 간이 시험지 개발

이인애,김재화,이희구,성창근,최인성,정태화 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-

대장균군 검사용 간이 시험지는 본 실험실에서 국내 최초로 고안, 개발하였으며 이 간이 시험지법은 현장 검사법의 하나로 대장균군이 내는 succinic acid dehydrogenase 때문에 tetrazolium salt 가 환원되어 적색 반점을 형성하는것을 이용한 방법으로서 이 간이 시험지의 제조는 대체로 종래의 표준 평판법과 거의 동일한 조성의 배지와 시약을 사용하여 여지에 흡착시킨 후, 건조시켜 (60℃) 멸균한 것으로 표준 평판법과 어떤 상관관계가 있는가를 검토하였다. 이 간이 시험지의 제조에서는 bile salt No. 3를 deoxycholate로 대체하여 제조 원가를 절감하였고, 또한 일본에서 현재 시판되고 있는 제품과 품질 비교시험을 하여 더 좋은 결과를 얻었으며 종래의 표준 평판법과 비교하였을 때도 오히려 표준 평판법(24-48시간 배양)보다 빠른 시간(16-20시간 배양)내에 판정할 수 있는 이점이 있으며, 표준 평판법에서는 없어서는 안될 배지나 배양 접시, pipette등의 자료및 기구가 일체 필요없고 언제 어디서나 현장에서 직접 시험할 수 있어 매우 간편하며 또한 저렴한 가격으로 제조할 수 있는 경제성이 높은 이점을 갖고 있다. The objective of this study was to develop a paper strip which could determine E. coli qualitatively and quantitatively in water, wastewater, drinks, or food. This paper strip method was a simple and rapid test method that determine E. coli by visual identification. In this study, nutrient culture media were formulated and characterized for optimum conditions. Paper strips were then prepared by impregnating into the media and dried at 60℃. The test procedure is quite simple to use. The paper strip was dipped into a sample, and excess sample was removed. The strip was then incubated at 37℃ for 16 to 20 hours and the number of colonies on the strip was counted. The color of the colony spots produced by microorganisms varied depending on the media formulation. Violet-red spots were produced by E. coli. The test method was simple, rapid and no special laboratory equipment was necessary for visual identification. Therefore, this test method is applicable to on-site tests such as field tests or home tests. The paper strip method was compared with the standard agar plate method and Japanese commercial product. The method of the economical preparation of test strips was studied for production on industrial scale.

Propylthiouracil 투여에 의한 갑상선 기능저하 흰쥐 조직에서 알코올 탈수소효소 활성의 변화

백상현,김동선,이창범,박용수,안유헌,김태화,기춘석,강주섭 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.2

Background: Thyroid hormone has been known to affect hepatic alcohol dehydrogenase (ADH) activity. Although the liver is the principal site of ethanol metabolism, stomach is also responsible in part for ethanol oxidation. The effects of thyroid hormone on ADH activity in gastric mucosa and other tissues of rats had not been previously examined. Method: The effects of thyroid hormone on liver, stomach, lung, and kidney ADH activities (nM of NADH/min/mg of cytosolic protein) have been investigated in male Sprague Dawley rats treated with propylthiouracil (50 mg/kg) for 14 days. Results: Whereas hepatic ADH activities were not changed by treatment with PTU(42.9(8.6 vs 45.2 (10.1), gastric ADH activities in PTU-treated rats increased by 258.8% of control rat (6.3 ( 0.6 vs 2.2 ( 1.2, p〈0.001). In the activities of other tissues, PTU treatment decreased lung ADH activity by 59.7% of control, and increased kidney ADH activities by 247.1% of control rats. Conclusion: These data suggest that hypothyroidism causes an increase of gastric alcohol metabolism, and that the increase of gastric ADH activity can partly restore the first pass metabolism of ethanol in hypothyroid rats.

대장균군 검사용 간이 시험지 개발

이인애,정태화,김재화,성찬근,이희구,최인성 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.1

대장균군 검사용 간이 시험지는 본 실험실에서 국내 최초로 고안, 개발하였으며 이 간이 시험지법은 현장 검사법의 하나로 대장균군이 내는 succinic acid dehydrogenase 때문에 tetrazolium salt 가 환원되어 적색 반점을 형성하는것을 이용한 방법으로서 이 간이 시험지의 제조는 대체로 종래의 표준 평판법과 거의 동일한 조성의 배지와 시약을 사용하여 여지에 흡착시킨 후, 건조시켜 (60℃) 멸균한 것으로 표준 평판법과 어떤 상관관계가 있는가를 검토하였다. 이 간이 시험지의 제조에서는 bile salt No.3를 deoxycholate로 대체하여 제조 원가를 절감하였고, 또한 일본에서 현재 시판되고 있는 제품과 품질 비교시험을 하여 더 좋은 결과를 얻었으며 종래의 표준 평판법과 비교하였을 때도 오히려 표준 평판법(24-48시간 배양)보다 빠른 시간(16-20시간 배양)내에 판정할 수 있는 이점이 있으며, 표준 평판법에서는 없어서는 안될 배지나 배양 접시, pipette등의 자료및 기구가 일체 필요없고 언제 어디서나 현장에서 직접 시험할 수 있어 매우 간편하며 또한 저렴한 가격으로 제조 할 수 있는 경제성이 높은 이점을 갖고 있다. The objective of this study was to develop a paper strip which could determine E. coli qualitatively and quantitatively in water, wastewater, drinks, or food. This paper strip method was a simple and rapid test method that determine E. coli by visual identification. In this study, nutrient culture media were formulated and characterized for optimum conditions. Paper strips were then prepared by impregnating into the media and dried at 60℃. The test procedure is quite simple to use. The paper strip was dipped into a sample, and excess sample was removed. The strip was then incubated at 37℃ for 16 to 20 hours and the number of colonies on the strip was counted. The color of the colony spots produced by microorganisms varied depending on the media formulation. Violet-red spots were produced by E. coli. The test method was simple, rapid and no special laboratory equipment was necessary for visual identification. Therefore, this test method is applicable to on-site tests such as field tests or home tests. The paper strip method was compared with the standard agar plate method and Japanese commercial product. The method of the economical preparation of test strips was studied for production on industrial scale.

한국 여성운동과 걸스카우트운동 : 과거·현재·미래 Yesterday, Present and Tomorrow

정세화,이동원,김미숙,박진숙,장화경,홍승아 이화여대 한국여성연구소 1988 여성학논집 Vol.5 No.-

One of the major implications of women's movements seems to lie in the fact that it tends to promote women's consciousness-raising and, thus, heighten social status of women in general. It would be very much the case when discussion centers around the emergence of Korean women's movements in modern sense. Due to the socio-political atmospheres of the lathe 19th century Korean society, its basic characteristics owe to strong tones of patriotic nationalism under the slogan of "learning is might enough to deal with the imperialistic foreign powers of the time." As such, ever since the introduction of women's enlightenment movements of the time, Korean women's movements have provided for the advancement of egalitarian society. However, assessing the effectiveness of programs that are practiced by the existing women's organizations in Korea, it deserves mention in that most programs are for the adult female members, assuming it would only be applicable to those adults. In this regard, it can be said that the Korean Girl Scout organization has worked a unique role to make up such voids in women's movements - most programs developed and exercised by the Girl Scout are reserved for those young girls. For instance, special activities like social services, field campaigns, and international gatherings would definitely work a profound impact in the formation of gender-role stereotypes of the youth. The present study tries to examine the Korean Girl Scout movement being the case of women's movement organization. Discussion will cover such topics as; historical reviews of Korean Girl Scout movement, principal organizational goals and its practice in real life, and internal dynamics of members between "staffs" and the ordinary members themselves-on perceptions of the movement, evaluation of leaderships, and the future orientations of the Girl Scout, etc. Various research methods are employed. Book reviews, in-depth interviews, questionnaires, and participant observations are utilized, when the respective measurement is required. Major findings of the study can be summarized as follows: (1) Numbers of Girl Scout membership have declined since 1980, while revealing its utmost pick upto mid-70s. In terms of regional distributions of the members, both Seoul and Kyung-Nam Branches are the most prosperous. Whereas commemoration ceremonies and camping works are considered most impressive programs, membership trainings and field works are the lowest. About 70 percent of the members show their length of activities in Girl Scout less than one year. Most members are involved in the activities based on self-decision in order to have social services, club memberships, or self actualizations. They are satisfied with most of the programs, especially when participating in the social service programs. (2) As for the staff members, about half of the leaders in the headquarters are newly appointed. Whereas majority of the 'field' staffs are young (in their 20s, or 30s), other leaders are considered old (most of them are in their 40s, 50s, or 60s). Half of the leaders are involved in the activities for less than 5 years. They are recruited by either colleagues' or office seniors' recommendations. Reasons to participate in the movement are to have self-actualized or to have opportunity for social services. All in all, leaders are satisfied with the Girl Scout activities mainly because they can see their youth members progressing and developing. (3) Several issues for the better organizational managements seem to be related. prevalence of aspirational differences between leaders and members tends to cause psychological burdens. Other problematics such as lack of free communication networks, tensions due to cohort differences, authoritarian leadership styles, and financial difficulties would hinder Girl Scout movement more promising and popular to all. Based on the research findings, two major suggestions might be in order. First, underlying rationales of the Girl Scout movement should be reoriented. A special emphasis on the creation of self-supportive 'androgynous individuals' would be the case (in this regard, an intensive program targeted for those pre-school-aged members would be the most effective). As a way to achieve the strategic reformulation, reliance on the interdisciplinary program (for example, coordination with the university) will be one possibility. Secondly, an organizational management should be sufficiently considered. provisions (tangible and intangible) for the 'field leaders' being the key leader group in the Girl Scout movement should be afforded enough to keep their participations voluntary and continuous.