인삼농축액의 잔류농약 제거기술 개발에 관한 연구

신영민(Yeong-Min Sin),손영욱(Yeong Uk Son),이선화(Seon Hwa Lee),정지윤(Ji Yoon Jeong),원영준(Young Jun Won),이창희(Chang Hee Lee),김우성(Woo Seong Kim),채갑용(Kab-Ryong Chae),홍무기(Moo Ki Hong) 한국농약과학회 2005 농약과학회지 Vol.9 No.1

해산물식품 중 식중독원인균의 오염패턴 및 저감화 방안

김순한,신영민,이명자,신필기,김미경,조정숙,이창희,이영자,채갑용,KIM Soon Han,Sin Yeong-Min,Lee Myeong Ja,Shin Pil Ki,Kim Mi Cyeong,Cho Jung Sook,Lee Chang Hee,Lee Young Ja,Chae Kab Ryoung 한국생명과학회 2005 생명과학회지 Vol.15 No.6

The contamination frequency of major foodborne pathogenic bacteria was investigated from 213 seafood samples including sliced raw fish and shellfish in Busan and CyeongNam province area. Tested microorganisms were Salmonella spp. Staphyloroccus aureus, Vibrio parahaemolyticus, Escherichia coli O157:H7, Bncillus cereus, Listeria monocytogenes and Campylobacter jejuni. The frequency of isolated microorganisms was V. parahaemolyticus (30.5%), B. cereus (9.9%), S. aureus (3.8%) and other pathogenic bacteria (1.4%). from July to October, total isolation rates were greater than 50% and V. parahaemolyticus was dominant among the microorganisms isolated. The bacteria isolation rate (49.2%) in raw seafoods including shellfishes was higher than one (28.9%) in sliced raw fish. V. parahaemelyticus isolates were resistant to ampicillin (96.9%), amikacin (29.2%) and tetracycline (27.7%), and B. cereus isolates were resistant to ampicillin (100%), Penicillin G (100%), rifampicin (71.4%) and tetracycline (14.3%). The growth of V. parahaemolyticus and B. rereus was greatly inhibited below $10^{\circ}C$, but increased at ambient temperature. Washing seafood with tap water showed to reduce total count of remaining V. parahaemolyticus. Thus temperature control under $10^{\circ}C$, sufficient washing and prompt eating appeared to reduce the risk of food poisoning by these bacteria in seafoods. 부산 및 경남일대에서 널리 유통되고 있는 생선회, 패류 등의 횟감류 213건을 대상으로 주요 식중독 원인균인 Salmonella spp., Staphylococcus aureus, Vibrio parahaemolyticus, Escherichia coli O157:H7, Bacillus cereus, Listeria monocytogenes 및 Campylobacter jejuni의 오염실태를 조사하였다. V. parahaermolyticus가 65건(30.5%), B. cerus가 21건(9.9%), S. aureus가 8건(3.8%) 및 기타 식중독균이 3건에서 검출되었다. 이를 월별 검출율로 비교해 보면, 7~10월에서 식중독균의 높은 검출율을 나타내었으며, 이러한 높은 검출율은 주로 V. parahaemolyticus에 기인된 것으로 조사되었다. 아울러 생선회에서의 검출율(28.9%) 보다는 패류 등의 기타 횟감류에서 더욱 높은 검출율(49.2%)을 나타내었다. V. parahaemolyticus의 경우 Ampicillin (96.9%), Amikacin (29.2%) 및 Tetracycline (27.7%)에 대해 내성을 나타내었으며, B. rereus의 경우는 Ampicillin (100%), Penicillin G (100%) 및 Rifampicin (71.4%)에 대해 높은 내성을 나타내었다. V. parahaemolyticus와 B. cereus의 생육은 보관온도 및 시간에 크게 영향을 받는 것으로 나타났으며, 수돗물 세척이 해산물에 잔존하는 V. parahaemolyticus의 균수를 크게 감소시키는 것으로 조사되어, 여름철 해산물 섭취로 인한 식중독 발생의 예방을 위해서 $10^{\circ}C$이하의 저온보관과 수돗물로써의 철저한 세척이 필수적인 것으로 판단되었다.

퍼지앤트랩 - 기체크로마토그래피(PT - GC)를 이용한 식품첨가물 중 1,4 - 디옥산 및 클로로히드린류 분석

조태용(Tae Yong Cho),신영민(Yeong-Min Sin),반경녀(Kyeong Nyeo Bahn),오세동(Se Dong Oh),이창희(Chang Hee Lee),이영자(Young Ja Lee),문병우(Byung Woo Moon) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.7

폴리소르베이트류, 염화콜린, 주석산수소콜린, 변성전분 및 향신료 중 1,4-디옥산 및 클로로히드린류의 분석법을 개발하고 확립된 방법으로 잔류량을 조사한 결과는 다음과 같다. 분석에 사용된 트랩으로는 Vocarb 3000이 적합하였고 이때의 검출한계는 DOX 1.38 μg, EPC 0.23 μg, PCH 3.30 μg, ECH 3.97 μg, DCP 20.43 μg이었다. 검체 온도증가와 무수 황산나트륨 농도 증가에 따라 분석감도는 증가하였으나 60℃ 이상에서는 상대표준편차(RSD, %)가 6%를 초과하여 재현성이 떨어져 분석온도를 50℃로 설정하였다. 폴리소르베이트류에서의 회수율은 염을 첨가하지 않고 측정하였을 때 EPC(66%)를 제외하고는 90%이상을 나타내었다. 염화콜린 및 주석산수소콜린의 경우에는 대부분 회수율이 100% 이상이었으나 주석산수소콜린의 EPC는 20%로 낮게 나타났다. 히드록시프로필인산이전분, 히드록시프로필전분 및 찰옥수 수전분에서는 검체를 가수분해한 후 회수율을 측정한 결과 검체종류별 큰 차이없이 회수율이 90% 이상을 나타내었다. 향신료의 분석에서는 무수황산나트륨을 첨가하여 분석한 결과 회수율이 90%이상이었다. 구매한 폴리소르베이트류, 염화콜린, 주석산수소콜린, 향신료 중 1,4-디옥산 및 클로로히드린류를 분석한 결과 트윈 80에서 DOX가 2.5 ppm 검출되었으나 이 외의 검체에서는 모두 불검출되었고 직접 제조한 히드록시프로필전분에서도 모두 불검출되었다. This study has been performed to develope a method for the simultaneous determination of 1,4-dioxane (DOX), epichlorohydrin (EPC), propylene chlorohydrin (PCH), ethylene chlorohydrin (ECH) and 1,3-dichloro-2-propanol (DCP) in polysorbates, chloline chloride, choline bitartrate, modified starch and spices by purge and trap-gas chromatography. Experimental design was used to select a suitable trap by measuring the limit of detection (LOD) and to investigate the effect of temperature and salt of extraction, and the percentage of recovery in various matrix. The LOD of DOX, EPC, PCH, ECH and DCP were 1.38 μg, 0.23 μg, 3.30 μg, 3.97 μg, 20.43 μg respectively, by means of using Vorcarb 3000 trap with 50℃ sample sparger. Excluding EPC, the recoveries of target compounds were above 90% in all matrix. Target compounds in polysorbates (17), choline chloride (5), choline bitartrate (5), modified starch (8) and spices (25) were not detected. But 2.5 ppm of DOX was detected in Tween 80.

병원성 Escherichia coli O157 : H7의 특이 항체 생산을 위한 Lipopolysaccharide 분리 및 정제

최학선(Hack-Sun Choi),신영민(Yeong-Min Sin),정숙현(Sook-Hyun Chung),박영민(Young-Min Park),안원근(Won Gun An) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.3

대장균 O157:H7의 백신 생산을 위해서 purity가 높은 lipopolysacharride 분리, 정제를 위한 실험을 실시하였다. 대장균 O157:H7 균주를 확인하기 위해서 shiga toxin을 생산할 수 있는 60 MDa plasmid를 분리하였고, PCR법에 의해 E. coli O157:H7 shiga-like toxin(Stx) 1, 2의 stx gene을 증폭하여 E. coli O157:H7의 특징(130 bp, 346 bp)을 확인하였다. E. coli O157:H7 LPS의 분리 정제는 페놀추출, 에탄올 분획 및 gel filtration의 간단한 방법을 사용하였다. 마지막으로 SDSPAGE와 silver nitrate 염색을 이용하여 LPS의 purity를 확인하였다. Escherichia coli O157:H7 cause hemorrhagic colitis and the extraintestinal complication of hemolytic-uremic syndrome, with their higher incidence occuring in children. Lipopolysaccharide (LPS) of E. coli O157:H7 is very important to make IgG anti-LPS with bactericidal activity. To identify the characteristic of E. coli O157:H7, we isolated 60 MDa plasmid and amplified stx genes of shiga-like toxin (Stx) 1, 2 of E. coli O157:H7 by polymerase chain reaction (PCR) method. Using the simple purification method which contained phenol extract, ethanol precipitation and gel filtration steps, the LPS of E. coli O157:H7 was isolated and purified. Finally, we confirmed the purity of LPS through SDS-PAGE and silver nitrate staining.

기체 크로마토그래피를 이용한 잔류농약 동시분석에 관한 연구

이동호(Dong Ho Lee),송병권(Byung Kwon Song),신영민(Young Min Sin),김형수(Hyung Soo Kim),김형일(Hyung Il Kim),손영욱(Young Wook Son),김재이(Jae I Kim),김우성(Woo Seong Kim) 한국환경분석학회 2000 환경분석과 독성보건 Vol.3 No.2

N/A

소화성 궤양에 있어서 혈청 Pepsinogen I 의 의의에 관한 연구

이선희(Sun Hee Lee),이동필(Dong Pil Lee),신영민(Young Min Sin),황성윤(Sung Yoon Hwang),송근암(Kun Am Song),조몽(Mong Cho),양웅석(Ung Suk Yang),문한규(Han Kyu Moon) 대한내과학회 1995 대한내과학회지 Vol.49 No.1

N/A Objectives: Peptic ulcer is heterogenous disease which has genetic or acquired etiologic factors and serum pepsinogen I (PG I) was reported as a marker of the genetic factor. In this study, the correlations of serum PG I level and natural course of peptic ulcer disease such as site, stage, recurrence and acquired factors such as smoking, H. pylori were evaluated. Methods: Fasting serum PG I levels were tested in 71 patients with peptic ulcer (Gastric ulcer 31, Duodenal ulcer 40) who had been confirmed by endoscopy, biopsy and 58 healthy control from January, 1991 to August, 1993 at Pusan National University Hospital. Serum PG I levels between ulcer and control group were compared and correlations of serum PG I level and natural course such as site, stage, complicaton, recurrence and acquired factors such as smoking, NSAID, H. pylori in ulcer group were observed. Results : 1) The patients with peptic ulcer showed significantly higher mean levels of serum PG I than those of the control group. Especially the mean levels of PG I of antral ulcer and duodenal ulcer were higher than ulcer of gastric body and angle. 2) The patients with active ulcer showed higher mean levels of serum PG I than healing stage ulcer. but there weas no statistical significance. 3) The patients with recurrence, smoking history, complications showed higher mean levels of PG I than those without recurrence, smoking history, complications. but there was no statistical significance. 4) The CLO test positive group of the duodenal ulcer patients showed higher mean levels of serum PG I than those of CLO test negative group. but there was no statistical significance. Conclusion: The above results suggest that serum pepsinogen I may be useful as a parameters of activity and recurrence and as a subclinical markers for acquired risk factors of the peptic ulcer.

라즈베리파이를 이용한 스마트 미러

이용현(Yong-Hyeon Lee),서주희(Joo-Hi Seo),신영민(Yeong-Min Sin),송영준(Young Joon Song) 대한전자공학회 2018 대한전자공학회 학술대회 Vol.2018 No.11

유통 한약재의 이산화황 함유량에 관한 연구

신영민,조태용,이광수,김성훈,박흥재,임동길,이창희,김우성,채갑용,이영자,최수영 한국환경과학회 2004 한국환경과학회지 Vol.13 No.12

This study was investigated the residual contents of sulfur dioxide on the 373 kinds of herbal medicine distributed from Korea, China, and Japan. A modified Monier-Williams method was described for the determination of SO₂ contents in herbal medicines. The residual contents of SO₂ were not detected at 221 products(59.8%) in total 373 products. Regardless of region, SO₂ contents were not found at Farfarae Flos, Zizyphi Spinosi Semen, Castanea crenata, and Strychni Ignatii Semen. But it's found at Asparagi Radix, Codonopsitis Radix, Lilii Bulbus, and Kaempferiae Rhizoma of every region collected the samples. Also, SO₂ contents were not detected at the herbal medicines which collected cultural fields of dometic. SO₂ contents ranged 11~3990 mg/kg(mean 152 mg/kg) at domestic samples, 11~3440 mg/kg(mean 603 mg/kg) at imported samples. After treated with water wash and hot water extraction, the reduction rates of sulfur dioxide were appeared with 24.4%~68.7% and 83.8%~100.0%, respectively. These data will be used to establish a criteron of residual sulfur dioxide in herbal medicines.

성인병에 대한 한방치료법(증치의학과 사상의학)에 관한 연구 : 고혈압에 대한 한방치료법(중치의학과 사상의학)에 관한 연구

박동일,김영균,안창범,이인선,김종원,권정남,장경전,이인선,이성근,장용우,신영민 동의대학교 한의학연구소 1999 동의한의연구 Vol.3 No.-

We had a result of the treatment as below when is devided Korean medicine Tx.. Western medical Tx., Cooperative Tx.. 1) At the improvement of BP control, an average in Korean medicine Tx. (from 170㎜Hg/100㎜Hg to 150㎜Hg/90㎜Hg), an average in Western medical Tr. (from 170㎜Hg/100㎜Hg to 130㎜Hg/80㎜Hg), an average in Cooperative Tx.(from 180㎜Hg/110㎜Hg to 130㎜Hg/90㎜Hg), generally all pars had improvements of BP control. But it is not significant of each case. 2) At the improvement of symptoms by the apologetics, Cooperative Tx. is profitable in cases of 'GanHwa',' DamEum', 'EumYangYangHer', 'EumHer'. Korean medicine Tx. is superior in case of 'GiChe'. 3) At the improvement of symptoms by a questionnaire, Korean medical Tx., Western medical Tx., Cooperative Tx. groups had improvements, but each practice group didn't have specific significance. Only it was somewhat profitable to Korean medical Tx. in the 210㎜Hg/110㎜Hg, Western medical Tx. in the 180㎜Hg/110㎜Hg, Cooperative Tx, in the 170㎜Hg/90㎜ Hg. 4) There are the apparent improvements in patients whose BP are over 200㎜Hg of Korean medical Tx. group, whose BP are over 190㎜Hg of Western medical Tx. group and whose BP are over 170㎜Mg of Cooperative Tx. group. There are the improvement of diastolic BP in 110㎜Mg(Korean medical Tx., Western medical Tx.) and 90㎜Hg(Cooperative Tx.). 5) At the improvement of Pulse pressure, generally Pulse pressure are decreased. There are similar improvements in all of Korean medical Tx., Western medical Tx., and Cooperative Tx.. 6) At the Symptomatic approvement according to ages, Korean medical Tx., Cooperative Tx. are somewhat good for his twentieth, and Western medical Tx. is somewhat good for his forties. 7) the correlation of Obesity-grade and BP, as Obesity-grade is higher as BP is higher, but there are no similarity in the improvement. 8) At the EAV improvements, as examination into correlation with the point of 1~3th, we could get results as below. There are high improvements of DRHTM, DRALM, DLLYM, DLLIM in Cooperative Tx. There are high improvements of DRPASI, DLLARI in the Korean medical Tx. group. There are high improvements of DRFADM in Western medical Tx. group.