5-FU 내성 대장암 세포주에서 생물학적 지표로서 단백 분해효소발현의 유용성

조몽(Mong Cho),최석렬(Seok Reyol Choi),이주일(Joo Il Lee),이철종(Cheol Jong Lee),김영식(Young S Kim) 대한내과학회 1999 대한내과학회지 Vol.57 No.3

N/A There have been many reports that colon cancer responds poorly to chemotherapy. Several classes of matrix metalloproteinases(MMPs) have been implicated in the process of invasion of epithelial and endothelial basement membranes in several steps of tumor invasion and metastasis. This study was performed to determine the biologic behavior and the histopathological characteristics of a 5-FU resistant colon cancer cell line. Methods : We performed several biologic assays including liver colonization assay, cell adhesion assay, invasion assay and zymogram for protease activity using parental HT29 cell and 5-FU resistant HT29 cell (HT29-FU cell). Results : In liver colonization assay, HT29-FU cell revealed a 2.5-fold increase in the liver weight and tumor burden compared with HT29 cell. HT29-FU cell showed moderate increase in adhesion and invasion assays in comparison to HT29 cell. HT29-FU cell revealed increased activity of MMPs and serine protease. Xenograft tumors of HT29-FU cell formed moderately differentiated adenocarcinoma with more glandular formations of mucin. Conclusion : The increased expression of MMPs in 5-FU resistant colon cancer cell can explain poor prognosis. These are potentially poor prognostic indicators in 5-FU resistant colon cancer. Consequently, it can be suggested that modulation of MMPs is needed to prevent invasion and metastasis in colon cancer by using inhibitors of these enzymes. (Korean J Med 57:323-332, 1999)

위암과 전이된 국소림프절의 세포 Glycocojugates 에 대한 Lectin 반응에 관한 연구

조몽(Mong Cho),양웅석(Ung Suk Yang) 대한내과학회 1992 대한내과학회지 Vol.43 No.2

N/A N/A

연구논문 : 만성 B형간질환 환자의 라미부딘 내성 예측인자

허정 ( Jeong Heo ),조몽 ( Mong Cho ),조병만 ( Byung Mann Cho ),이선미 ( Sun Mi Lee ),김태오 ( Tae Oh Kim ),김광하 ( Gwang Ha Kim ),강대환 ( Dae Hwan Kang ),송근암 ( Geun Am Song ) 대한간학회 2007 Clinical and Molecular Hepatology(대한간학회지) Vol.13 No.2

연구논문 : 라미부딘 내성 만성 B형간염 환자에서 아데포비어 단독치료의 효과와 아데포비어에 대한 유전자형 내성의 발생 양상

문재현 ( Jae Hyeon Moon ),조몽 ( Mong Cho ),윤기태 ( Ki Tae Yoon ),배정호 ( Jung Ho Bae ),허정 ( Jeong Heo ),김광하 ( Gwang Ha Kim ),강대환 ( Dae Hwan Kang ),송근암 ( Geun Am Song ) 대한간학회 2008 Clinical and Molecular Hepatology(대한간학회지) Vol.14 No.4

목적: 아데포비어(Adefovir dipivoxil, ADV)는 뉴클레오타이드 유사체로 야생형 B형간염바이러스(HBV)뿐만 아니라 라미부딘 내성 HBV 변이형도 억제한다. 라미부딘 내성 환자에서 아데포비어의 내성 발생률이 높다고 알려져 있지만 이에 대한 연구는 아직 부족하다. 본 연구에서는 라미부딘 내성 HBV 감염 환자를 대상으로 아데포비어를 투여할 때 치료효과 및 유전자형 내성의 발생 양상을 조사하고자 하였다. 대상 및 방법: 이 연구는 라미부딘 내성 만성 HBV감염 환자로 아데포비어 단독치료를 시행하였던 124명을 대상으로 하였다. 아데포비어의 치료효과로 누적 혈청 ALT 정상화율과 혈청 HBV DNA 감소 정도 및 일정 수준(검출역치 2×10(4) IU/mL와 2×10(2) IU/mL) 이하로 감소하는 환자의 누적 비율을 확인하였다. HBeAg 양성환자의 경우에는 누적 HBeAg 음전율 및 혈청전환율을 확인하였다. HBV 중합효소의 변이는 직접 염기서열 분석법을 이용하여 확인하였다. 결과: 누적 혈청 ALT 정상화율은 12개월 및 24개월째 각각 69.4%와 75.5%였다. 아데포비어 12개월 및 24개월 투여 후 혈청 HBV DNA가 2×10(4) IU/mL 이하로 감소한 환자는 각각 79.8%, 89.2%였으며, 2×10(2) IU/mL 이하로 감소한 환자는 44.2%, 59.0%였다. 아데포비어 단독투여 12개월 및 24개월째 혈청 HBV DNA는 각각 평균 3.24 및 3.04 log10IU/mL로 아데포비어 단독치료 전과 비교하여 의미 있게 감소하였다(P<0.001). 아데포비어 투여 12, 24개월 후 변이형 rtN236T는 각각 25%, 42%, rtA180T/V는 각각 64%, 83%였다. 결론: 라미부딘 내성 만성 HBV 감염 환자에서 아데포비어 치료는 효과적이었지만 HBV 역전사효소의 유전자형 내성 발생률이 높았다. Background/Aims: Adefovir dipivoxil (ADV) is a nucleotide analogue that inhibits wild-type hepatitis B virus (HBV) and lamivudine (LMV)-resistant HBV mutants. The aim of this study was to elucidate the efficacy of ADV monotherapy and the incidence of genotypic resistance to ADV in patients with LMV-resistant chronic HBV infection. Methods: This study involved 124 patients with chronic HBV infection who had received ADV monotherapy due to the presence of LMV-resistant HBV mutants. The efficacy of ADV was evaluated by the normalization of serum alanine aminotransferase (ALT) level and by the reduction of serum HBV DNA level (with cutoff levels of 2×104 IU/mL and 2×10(2) IU/mL). The cumulative rate of HBeAg loss or seroconversion was assessed in HBeAg-positive patients. The development of mutations in the reverse trancriptase region of HBV DNA polymerase was evaluated by direct sequencing analysis during ADV monotherapy. Results: The mean serum HBV DNA level was 5.94 log10IU/mL. At 12 and 24 months after ADV monotherapy, the cumulative rates of serum ALT normalization were 69.4% and 75.5%, respectively, and those of serum HBV DNA reduction were 79.8% and 89.2% for a cutoff level of 2×10(4) IU/mL, and 44.2% and 59.0% for a cutoff of 2×10(2) IU/mL. The mean serum HBV DNA levels at 12 and 24 months were significantly lower than baseline, at 3.24 and 3.04 log10IU/mL, respectively (P<0.001). At 12 months after ADV treatment, the cumulative rates of HBeAg loss and seroconversion were 15.8% and 10.5%, respectively, and the rtN236T and rtA181T/V mutants in HBV DNA polymerase were identified in 25% and 64% of patients, respectively. Conclusions: Although ADV monotherapy is effective, it leads to a high rate of mutations of HBV DNA reverse transcriptase gene in patients with chronic HBV infections who have LMV-resistant HBV mutants. (Korean J Hepatol 2008;14:503-512)

간흡충 감염가토의 담관에 대한 병리조직학적 변화 및 Lectin 반응에 관한 연구

양웅석(Ung Suk Yang),조몽(Mong Cho),백태현(Tae Hyun Paik),김상현(Sang Hyun Kim),주형준(Hyung Jun Joo),이동필(Dong Phil Lee),송근암(Geun Ahm Song),문한규(Han Gyu Moon) 대한소화기학회 1993 대한소화기학회지 Vol.25 No.6

N/A Histopahological and histochemical studies were done in the bile ducts of clonorchis sinensis (CS) infested rabbits. The duration of clonorchis sinensis infestation in the rabbits was between 12 and 17 months and the number of clonorchis sinensis metacercariae which were infested orally was 50-150 per rabbit. Hematoxylin-eosin and avidin-biotin peroxidase complex lectin staining methods were used for this study. Pathological changes of the bile ducts of CS-infested rabbits were papillarv and adenomatous hyperplasia, goblet cells and mucus cells rnetaplasia, ductal wall fibrosis and infitra tion of eosinophils in the ductal wall. These pathological changes were noted both in peripheral and central protion of bile duct. The pathological changes of hyperplasia and metaplasia of ductal epithelium were more marked in the deep gland than surface one, and dysplasia of the epithelium was more marked in the suface gland than deep one. In clonorchis sinensis infested rabbits, the lectins of Soybean agglutinin (SBA), Dolichos biflours agglutinin (DBA) and Ulex europaeus agglutinin (UEA I) were labelled predominant]y in the luminal suface, supranuclear protion of epithelium, goblet cells, hyperplastic cells and metaplastic cells of deep gland . SBA and DBA were labelled weakly to intermediate and UEA I was labelled weakl y to strong, but Peanut agglutnin (PNA) was ]abelled very weakly to those sites in CS-infested rabbits. In non CS infested rabbits, lectins of SBA, DBA, and UEA I were only weakly labelled in the luminal surface of epithelium. In this study, IJEA i which binds specifically glycoprotein of fucose was more pronounced in rabbits infested with CS and so it is postulated that increased activity of fucosyl transferase should be associated with CS infestation.

대장암 및 대장선종에서 p16 유전자의 과메틸화

김도하 ( Do Ha Kim ),조몽 ( Mong Cho ),김대현 ( Dae Hyun Kim ),주광로 ( Kwang Ro Joo ),박능화 ( Neung Hwa Park ),양웅석 ( Ung Suk Yang ) 대한내과학회 2003 대한내과학회지 Vol.64 No.4

목적 : 최근 종양억제유전자 중의 하나인 p16 유전자의 불활성화가 여러 암의 발생에 관여되고 있는 것이 확인되었다. 대장암의 발암과정에서 p16 유전자의 불활성화는 p16 promoter의 과메틸화가 중요한 기전으로 보고되었지만 현재까지 국내에서는 이에 대한 보고가 없는 실정이다. 따라서 본 연구에서는 대장선종과 대장암에서 p16 유전자의 메틸화를 조사하여 대장암 발암과정에서의 역할을 살펴보고 나아가 대장암의 여러 임상 양상 및 병기에 영향을 미치는가 Background : De novo methylation and transcriptional silencing of p16 gene is one of the main pathways in inactivation of p16 gene in colon neoplasm. But there has been no study about that in Korea yet. The aims of this study were to determine p16 methyla

간세포암종의 근치적 절제술 후 조기에 발생한 다발성 간 내 재발 및 간 외 전이 환자에서 장기 생존을 보인 증례 1예

정동일 ( Dong Il Jeong ),홍영미 ( Young Mi Hong ),조몽 ( Mong Cho ),윤기태 ( Ki Tae Yoon ) 대한간암학회 2015 대한간암학회지 Vol.15 No.2

The most effective therapy in patients with hepatocellular carcinoma (HCC) is curative resection of liver cancer. However, the long-term prognosis is not good in high intrahepatic recurrence. The recurrence rate after curative resection of HCC is 70-100% and the average survival time was about 15.8 months in early relapse after curative resection. Extra hepatic metastases in HCC is associated not uncommon, in general, lungs are the most common organ, lymph nodes, bone, and adrenal gland is relatively often observed. Extrahepatic recurrence after curative resection of HCC has been reported in 14-26% of the total recurrent cancer. Most of the recurrent cancer is multiple and the median survival time of patients after the extrahepatic recurrence is about 4-5 months it found that the prognosis is very poor. Therefore, the authors report a case showed long term survival in patients with early intrahepatic recur and extrahepatic metastasis after curative resection of HCC. (J Liver Cancer 2015;15:132-135)

만성활동성간염에 대한 간추출제제의 임상효과

문한규(Han Kyu Moon),심원보(Won Bo Shim),김지원(Ji Won Kim),조몽(Mong Cho),윤명순(Myung soon Yun) 대한소화기학회 1984 대한소화기학회지 Vol.16 No.2

N/A The purified protein-free liver extracts have been used for past many years in the treatment of both acute and chronic liver diseases. To evaluate the therapeutic effect of liver extract, Adelavin, this clinical study was carried out in 25 HBsAg positive patients with histologically proven chronic active hepatitis. 4ml of Adelavin mixed with 5% dextrose in water 500 ml were infused intravenously daily for 4 weeks. The following results were obtained. 1) The subjective symptoms were improved after 4 weeks-administration by improvement rate 70% in fatigue, 60% in anorexia, 75% in nausea, 45% in indigestion, 67% in abdominal discomfort. 2) There were significant improvements in SGOT, SGPT, alkaline phosphatase, total protein after 2 weeks-administration than preadministration(p<0.01), in albumin after 4 weeks- administration (p<0.001), in prothrombin time after 3 weeks-administration (p<0. 05) 3) In serum protein electrophoresis, there was significant increase of albumin after 4 weeks- administration (p<0.001) and significant decrease of gamma globulin after 4 weeks-admini stration (p<0.001). 4) In radioimmunoassay, there was no change of Hepatitis B marker after 4 weeks-administration. 5) There was not any abnormality in complete blood count and urinalysis after 4weeks- administration, but the transient nausea was found in one patient, the mild acne in two patients, the skin rash with fever in one patient.

간 이식 후 재발한 간세포암의 Sorafenib 치료 4예 보고

홍영미 ( Young Mi Hong ),윤기태 ( Ki Tae Yoon ),조몽 ( Mong Cho ),강대환 ( Dae Hwan Kang ),김형욱 ( Hyung Wook Kim ),최철웅 ( Cheol Woong Choi ),박수범 ( Su Bum Park ),허정 ( Jeong Heo ),우현영 ( Hyun Young Woo ),임원 ( Won Lim 대한소화기학회 2015 대한소화기학회지 Vol.65 No.4

With an increasing number of patients with hepatocellular carcinoma (HCC) undergoing liver transplantation (LT), tumor recurrence remains the main limiting factor for long-term survival. Although sorafenib is available for advanced HCC, there is still a lack of data on the use of sorafenib for treatment of recurrent HCC after LT. Here, we report on four cases of the use of sorafenib for treatment of recurrent HCC after LT. The median time of recurrence from LT was 4 months (range, 1-16 months). Two of the four evaluated patients showed stable disease, which was the best response and the duration of stabilization was 11 months and 5 months, respectively. One patient also experienced stable disease and remained in stable disease without sorafenib therapy for 29 months and the total duration of stabilization was 38 months. The remaining patient showed partial response but stopped treatment due to radiological tumor progression during treatment. Although all cases were high risk group for recurrence such as above Milan criteria, vascular invasion and tumor biology, clinical outcomes showed some good results. Therefore, sorafenib may be an acceptable treatment option for recurrent HCC after LT. (Korean J Gastroenterol 2015;65:246-251)

Mallory-Weiss syndrome의 내시경적 진단과 치료

송근암(Geun Am Song),김태오(Tae Oh Kim),강대환(Dae Hwan Kang),조몽(Mong Cho),양웅석(Ung Suk Yang),배상문(Sang Moon Bae),류수형(Soo Hyung Ryu),안준협(Jun hyup An),이성훈(Seong Hwun Lee) 대한내과학회 2000 대한내과학회지 Vol.58 No.5

N/A Background : Mallory-Weiss syndrome is a laceration of gastroesophageal junction due to abruptly increased intraabdominal pressure. Bleeding from Mallory-Weiss tears stops spontaneously within 2-3 days without specific therapy in 80-90% of cases, but in some cases, aggressive treatment is required due to massive bleeding. Methods: Among two hundreds and fifteen cases of upper gastrointestinal bleeding from January 1997 to January 1999, twenty three cases (10.7%) were diagnosed as Mallory-Weiss syndrome by endoscopy. We assessed the site, number, coexisting diseases, precipitating factors and bleeding lesion according to the time interval after the tears. After supportive care or specific therapy, we performed follow-up endoscopy to evaluate the healing of the lesions. Results: The mean age was 49.1 years and male:female ratio was 4.8:1. The most common precipitating factors were nausea, vomiting and alcohol drinking. In twenty cases, coexisting diseases such as gastritis and esophageal varix were detected. As for the number of tears, one tear was the most common (69.6%), while two tears were identified in five cases and three were in two cases. Thirteen cases of the Mallory-Weiss tears were located on the gastroesophageal junction, seven cases on the lower esophagus, one case on the cardia and two cases from lower esophagus to cardia. Eighteen cases were diagnosed by endoscopy within 24 hours after bleeding, of whom fourteen cases had active bleeding. Among four cases diagnosed after 24 hours, endoscopic finding revealed active bleeding in two cases and blood clots in the other two cases. We treated thirteen cases with supportive care, one case with hypertonic saline injection and nine cases who had active bleeding or deep and long tears with endoscopic band ligation. One or two weeks later, we performed follow-up endoscopy, and no bleeding was detected in all cases. Conclusion: We diagnosed twenty three cases of Mallory-Weiss syndrome by endoscopy and treated all cases with supportive care or endoscopic band ligation successfully.(Korean J Med 58:542-547, 2000)