DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

비매너 주차 단속시스템

박상민(Sang-min Park),손병수(Byung-Soo Son),김명식(Myung-sik Kim),최병윤 한국정보통신학회 2021 한국정보통신학회 종합학술대회 논문집 Vol.25 No.1

주차장에서 일어날 수 있는 비매너 주차로 인한 충돌을 사전에 예방하기 위한 단속시스템이다. 주차장에서 일어날 수 있는 장애인, 전기차 전용 주차구역에 일반차량 주차, 2개 차선을 물고 주차하는 차량이 있다. 위와 같은 차량을 딥러닝 객체인식 기능을 통해 비매너 주차를 감지하여 알려준다. 비매너 주차 상황이 찍힌 사진이나 영상을 학습데이터로 사용하여 상황을 인식할 수 있도록 학습데이터를 제작하고 그 상황을 인식하여 비매너 주차 유무를 판단한다. 주차장의 환경을 좀 더 쾌적하게 함으로써 주차장 이용자간 충돌을 줄이는데 목적이 있다. It is a enforcement system to prevent collisions caused by unmanageable parking that may occur in parking lots. There are handicapped people who can get up in parking lots, general vehicles parked in electric vehicle parking areas, and vehicles parked in two lanes. The vehicle above is detected and notified through the deep learning object recognition function. By using a picture or video of an unmanageable parking situation as learning data, the learning data is produced so that the situation can be recognized, and the situation is recognized to determine the presence or absence of unmanageable parking. The purpose is to reduce collisions between parking lot users by making the environment of the parking lot more comfortable.

이명직의 성서강해 연구

박명수(Park Myung-Soo) 한국기독교역사연구소 2004 한국기독교와 역사 Vol.21 No.-

The purpose of this paper is to describe and analyse several Bible expositions (Matthew, Daniels, Songs of Solomon, Romans, Ruth, Hosea, Revelation) written by Lee, Myung-Chick during 1920s. Rev. Lee has been called the father of the Holiness Church for long time. He had been the chief administrator of the Holiness Church and the principal of the Kyung-Sung Bible School (now, Seoul Theological University) for several decades. However, he was also the famous Bible expositor in Korean Church. He wrote many Bible expositions in his life and published them in monthly the Living Water. His exposition of the Bible had influenced the Holiness Church as well as the Korean church in general. In 1920s, Korean society confronted secularization of western culture, communism from Russia, and liberal theology of western church. In this context, Lee had tried to maintain the Full (or pure) Gospel of the Bible. He had criticized secularization very strongly, considered communism as anti-Christ, and attacked liberal theology as a threat of Christianity. After description and analysis of the Bible exposition, the writer concludes the main characteristics of the Bible expositions by Lee, Myung-Chick as followings. First, Rev. Lee tried to read the Old Testament from Christological perspectives. He found many Christological figure in the Old Testament. Second, he stood in the tradition of the Wesleyan Holiness movement, key teaching of which is the Holiness experience by the Baptism of the Holy Spirit. Third, his framework of the Bible interpretation is premillennialism. For Rev. Lee, Chiristian should live in the shadow of the second coming of Christ. Fourth, his emphasis on holiness and the second coming of Christ led to attack secularization as anti-Christ. Christian should not follow this world. Here Holiness movement considered as anti-secularization movement. Finally, Rev. Lee explained the Bible from woman’s perspectives. Of course, Rev. Lee is not feminist. However, all Christian should be a bride of the coming Christ, the bridegroom of all believers. For him, Daniels, Ruth, and the woman of Solomon in the Songs were the examples of true bride of Christ. Church history sometimes considers as history of the Bible interpretation. The Bible interpretation principles of Rev. Lee had heavily influenced the Korean Holiness Church.

捻轉法이 竝行된 鍼刺戟 및 電鍼刺戟이 鎭痛效果에 미치는 影響

Park, Dong-Seok,Kim, Ji-Hoon,Min, Byung-Il,Oh, Soo-Myung 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Dong-Seok Park¹, Ji-Hoon Kim¹ and Byung-Il Min, Soo-Myung Oh,³¹Department of Acupuncture and Moxibustion, College of Medicine, ²Department of Physiology, College of Medicine, And ³East-West Medical Research Institute, Kyung Hee University,Seoul, Korea. Analgesic Effect of Manual Acupuncture and Electroacupuncture combined with Twirling Method. Proceeding of International Symposium on East-West Medicine Seoul. 187-200, 1999.-Twirling the needle (TN) is one of several needing methods frequently used for acupuncture in the oriental medicine. The present study was conducted to see if TN enhanced the antinociceptive effect produced by plain-or electroacupuncture in the rat tail flick test. Rats (Sprague-Dawley, 200-300g)lightly anesthetized with thiopental sodium(40mg/kg, i.p.) were used in the tail-flick test. The basal reaction time for tail-flick was 2 sec. For "plain" acupuncture(PA), a needle was inserted for 20 min into a Chock-samni (ST36) acupuncture point which (is located at the anterior tibial muscle and about 10mm below the knee joint). For electroacupuncture(EA), train-pulses(3 Hz,0.3-ms pulse width, 0.2-0.3mA) were applied to the inserted needle for the period of insertion. For combining TN with either PA or EA, TN was performed during the each acupuncture period. Two methods for TN were used; twirling 3 times for 1 second persisted for 1 min in every 5 mins(long-duration and long-interval;LDLI) and twirling 3 times for 1 second persisted for 10 sec in every 1 min(short-duration and short-interval;SDSI). PA and EA produced an increase in tail flick latency (TFL); peak increases were 21.3±5.1% and 42.1±17.3% of the pre-acupunture control, respectively, and occurred immediately after cessation of 20min-acupuncture. Performing TN combined with PA increased TFL more than PA itself by showing a greater peak increase in TFL when performed with a SDSI-TN than with a LDLI-TN(60.6±12.5% and 42.7±22.7% of the pre-acupuncture control, respectively). Performing TN combined with EA also increased TFL more than EA itself and a greater peak increase in TFL was observed with a SDSI-TN as compared to a LDLI-TN(77.5±13.8% and 67.3±14.0% of the pre-acupuncture control, respectively). These results indicate that TN enhances both PA-and EA-produced antinociception where an greater enhanced antinociception than a LDLI-TN. It is suggested that performing a SDSI-TN combined with EA seems to be one of the most effective ways to produce analgesia.

이명직 목사와 일제시대 한국성결교회의 부흥운동

박명수 ( Myung Soo Park ) 현대기독교역사연구소 2002 성결교회와 신학 Vol.7 No.-

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

발달성 고관절 이형성증 환자에 발생된 고도의 탈구에 대한 전자하부 단축 절골술을 통한 인공고관절 치환

박명식,서동주,박종혁,오상수,황병연 대한고관절학회 2004 Hip and Pelvis Vol.16 No.1

사회환경교육의 교육과정 표준화 모형 및 통합프로그램 개발연구

박석순,최돈형,성효현,허명,강명희,이숙임,남상준 이화여자대학교 환경문제연구소 1998 이화환경연구 Vol.2 No.-

This paper presents the development of standardized modules and an integrated program which will be used for social environmental education. The study objectives are to develop modules which are based on various environmental topics, to relate the regional environmental topics to the educational modules and program, and to include the demand of students in the modules and program. Literature and questionary surveys, as well as, module development method were utilized as research tools. The titles of developed modules are "The Green Plant Earth," "Unexpected Resources: Save Resources and Recycle", "Water as the Mother of All Lives", "The Blue Sky with Clean Air", and "Soil as the Home of All Living and Non-living Things". It is hoped that the developed modules and program are utilized as course materials and curriculum for social environmental education in various educational institutes. The study results will be materialized as multimedia in near futher.

글루코만난을 첨가하여 제조한 패티가 고지방 식이로 유도된 비만 흰쥐의 체중 감소 및 영양상태에 미치는 영향

박수진,강명화 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.1

This study was carried out to investigate the effect of body weight reduction of patty containing glucomannan in high fat diet-induced obese rats. Male Spraque-Dawley rats were randomly assigned to control and high fat diets groups for 4 weeks. After 4 weeks, the control and high fat diet groups were rearranged into 5 groups by different levels of patty containing glucomannan (60%). After 5 weeks, serum and whole blood levels were analyzed. Obesity index was significantly lower in the group fed patty containing glucomannan than that of high fat diet groups. The status of white blood cells in hematological examination was significantly higher in rats fed high fat diet and not significantly different from those fed patty. Serum albumin levels were not significantly different, while serum glucose levels were significantly different among groups. Serum Triglyceride and total cholesterol levels were the highest in rats fed high fat diets, while the lowest in rats fed patty containing glucomannan.

착유우 사료에 대한 Aspergillus oryzae 발효물질 첨가가 in vitro 건물, 섬유소 및 단백질 소화율과 발효액의 pH에 미치는 영향

명윤아,박덕섭,이수기,박종수,김용국 충남대학교 농업과학연구소 2002 농업과학연구 Vol.29 No.2

본 연구는 Aspergillus oryzae 배양물이 in vitro 건물, 섬유소 및 단백질 소화율과 pH에 미치는 영향을 구명하고자 실시하였다. 균종 Aspergillus oryzae는 한국 생명공학연구원에서 분양받았으며, 본 연구에 사용된 사료는 시중 유통중인 착유우용 배합사료와 TMR 사료로서, 일반성분 분석은 (주)우성사료 중앙실험실에서 실시하였고, 건물, 섬유소 및 단백질 소화율 실험은 충남대학교 실험실에서 실시하였다. 배합사료와 TMR에 Aspergillus oryzae 배양물 0, 1.0, 2.0 및 3.0%를 첨가한 후, Holstein종 착유우로부터 채취한 반추위액에 시료 2.0g 내외를 넣어 진탕배양기에서 24, 48 및 72시간 소화시켰다. 침전물에 0.2% pepsin이 들어있는 0.1N HCl 30ml를 넣고 39℃ incubator에서 48시간 소화시키고 나서, 마지막으로 침전물을 60℃의 건조기에서 48시간 건조시켰다. 실험은 3회 반복하여 실시하였다. 건물 소화율은 Aspergillus oryzae 발효물을 첨가하지 않은 대조구에 비해 24시간 배양시킨 배합사료의 경우 2.1%(63.1%), 9.7%(68.5%) 및 9.0%(68%) 개선되었고, TMR은 4.8%(60.0%), 6.4%(61.1%) 및 2.9%(58.8%)의 개선효과가 있었다. 이와 대조적으로, 48시간 및 72시간 배양시킨 시험구의 건물 소화율에 Aspergillus oryzae 배양물이 미치는 영향은 24시간 배양시보다 상대적으로 낮은 경향을 보였다. 섬유소에서 소화율에서 Aspergillus oryzae가 조섬유, ADF 및 NDF 소화율을 유의적으로 개선시킨다는 것을 알 수 있었다. 배합사료에서 3% 첨가구의 경우 24시간 배양시 13.3%(53.3), 72시간 배양시 2.4%(54.6%)까지 증가되었다. 하지만, 첨가수준에 따라 소화율이 높아졌음에도 불구하고 첨가수준별 개선효과에는 유의적인 차이가 없었다. 단백질 소화율은 Aspergillus oryzae 배양물 첨가에 의해 배합사료에서 0.4%(79.7)에서 9.4%(71.8%)의 유의적인 개선효과를 나타내었지만, 2.0%와 3.0% 첨가구간의 유의적인 차이는 인정되지 않았다. TMR의 경우에는 4.0%(70.4%)에서 6.3%(65.1%)의 유의적인 소화율 향상을 나타내었지만 2.0%와 3.0% 첨가구간의 유의적인 차이는 인정되지 않았다. 본 연구에서, pH 처리구간 유의적인 차이는 없었으나, Aspergillus oryzae 배양물 첨가에 따라 pH가 약간 감소하는 경향을 보였다. 그러므로 본 발효물은 pH에는 영향을 미치지 않으나 건물, 섬유소 및 단백질 소화율 향상에는 다소 효과가 있는 것으로 결론을 내릴 수가 있다. This study was conducted to examine the effects of an Aspergillus oryzae fermentation culture on the in vitro digestibilities of dry matter, crude fiber, acid detergent fiber (ADF), neutral detergent fiber (NDF), crude protein and pH in vitro experiment of diets for dairy cows. A fungal species, Aspergillus oryzae was supplied by Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea (KCTC 1229). The experimental diets were commercial compound feed(concentrate) and total mixed ration (TMR) for lactating cows, of which chemical analyses were determined at Research and Development Institute, Woosung Feed Co.. Ltd.. while the digestibilities were done at the laboratory of Chungnam National University. Aspergillus oryzae culture products were added to compound feed and TMR at the rate 0, 1.0. 2.0, 3.0% respectively. The experimental diet with the rumen fluid sampled from Holstein fresian milking cows were used and digested for 24 hrs, 48hrs and 72hrs in the shaking incubator. The residues of the digesta were digested for 48hrs in the incubator in which put 30㎖ of 0.1N HCI with 0.2% pepsin at 39℃. The final precipitates were dried for 48hrs in the drier at 60℃. These experimental procedures were triplicated to determine the in vitro digestibility of dry matter. crude fiber. ADF, NDF, crude protein and pH. Compared to control diet, not added Aspergillus oryzae, the DM digestibility of fungal diets were improved 2.1%(63.1%), 9.7%(68.5%) and 9.0%(68.0%) for 24 hour fermentation in compound feed while 4.8%(60.0%), 6.4%(61.1%) and 2.9%(58.8%) in TMR. On the contrary. for 48 hour and 72 hour digestibilities, the effects of Aspergillus oryzae culture on the digestibility of dry matter were relatively lowered compared to 24 hour digestibility. Referring to the digestibility of dietary fiber, Aspergillus oryzae was believed to significantly improve digestibilities of crude fiber, ADF and NDF. Those were increased up to 13.3%(53.3%) for 24 hour fermentation while 2.4%(54.6%) for 3.0% added for 72 hour fermentation in compound feed. However, there were no signifiant differences among the treatments for the inclusion rate of Aspergillus oryzae, even though the more inclusion rate, the better digestibility. The protein digestibilities were significantly unproved from 0.4%(79.7%) to 9.4%(71.8%) by adding Aspergillus oryzae into compound feed. However, there were no significant differences between the two experimental diets. 2.0% and 3.0% Aspergillus oryzae included diets. In case of TMR, the protein digestibilities were significantly unproved from 4.0%(70.4%) to 6.3%(65.1%) by adding Aspergillus oryzae. However, there were no significant differences between the two experimental diets, 2.0% and 3.0% Aspergillus oryzae included diets. In this study, there were no significant differences among the treatments in pH. On the contrary, there were slightly decrease in pH by adding Aspergillus oryzae into experimental diets but not significant. Summarizing the results of this examination, Aspergillus oryzae fermentation culture is believed to improve the digestibilities of dry matter, fiber and crude protein in cattle diets. However, more detailed research for the mechanism of the fungal culture is required to improve ruminal environment.