연 X-선 현미경을 이용한 금 나노입자 세포영상

권영만,김한경,김경우,김선희,윤홍화,천권수,강성훈,박성훈,정선관,윤권하 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

연 x-선 현미경은 ‘물의 창’ 영역(2.3~4.4 nm)의 파장을 이용하여, 수십 nm의 분해능으로 세포를 파괴하지 않고 살아있는 상태에서 세포의 내부구조를 관찰할 수 있어 가시광선현미경과 전자현미경을 단점을 보완하는 특징을 갖는 세포 생물학 연구에 적합한 현미경이다. 그러나 기존 연 x-선 현미경은 광원으로 방사선 가속기를 이용하기 때문에 사용이 제한적이었다. 이에, 본 연구에서는 2.88 nm의 연 x-선을 광원으로 사용하는 소형 연 x-선 현미경을 이용하여, 내포작용에 의해 금 나노입자를 포획한 HT1080과 MDA-MB 231 세포의 영상을 약 60 nm 분해능으로 획득하였다. 금 나노입자의 세포에 대한 독성을 제거하기 위하여 폴리에틸렌 글리콜을 캡핑하였고, 2.88 nm 파장의 연 x-선에 대하여 충분한 조영효과로 인하여 세포영상에서 뚜렷한 대조도를 나타내었다. 내포작용에 의해 액포에 포함되어 있는 다양한 크기의 금 나노입자 군집을 확인하였으며, 세포내부의 액포의 분포상태도 관찰할 수 있었다. 따라서 고분해능을 가진 소형 연 x-선 현미경을 이용하여 금 나노입자를 세포내의 미세기관이나 특정 단백질에 표지하면 연 x-선에 대한 조영효과의 증가에 의하여 더욱 유용한 정보를 획득할 수 있을 것으로 생각한다. A compact soft x-ray microscope operated in the ‘water window’ wavelength region (2.3~4.4 nm) was used for observing cells with nano-scale spatial resolution. To obtain cellular imaging captured with colloidal gold nanoparticles using a compact soft x-ray microscope. The colloidal gold nanoparticles showed higher contrast and lower transmission more than 7 times than that of cellular protein on the soft x-ray wavelength region. The structure and thickness of the cell membrane of the Coscinodiscus oculoides (diatome) and red blood cells were seen clearly. The gold nanoparticles within the HT1080 and MDA-MB 231 cells were seen clearly on the soft x-ray microscopy. The gold nanoparticles were aggregated within vesicles by endocytosis.

핸드스프링 몸펴 앞공중1회 비틀기 동작의 소요시간 및 각운동량 분석

권오석,윤양진,서국웅 한국운동역학회 2002 한국운동역학회지 Vol.12 No.2

남자 기계체조 국가대표 선수 3명과 대학선수 3명을 대상으로 핸드스프링 몸펴 앞공중1회 비틀기 동작을 구간 및 국면별로 분석하고 동작의 숙련도에 따라 역학적 변인이 집단 간 어떠한 차이를 보이는지를 구명하기 위하여 Kwon3D 프로그램을 활용하여 분석한 결과 다음과 같은 결론을 얻었다. 시간요인에서 보폭을 크게 딛는 것이 손을 빠르게 지지 할 수 있고, 핸드스프링 체공시간은 짧게 하여야 발구르기의 압력을 높여 비틀기동작에서 체공 소요 시간이 길어지므로 비틀기 수행의 완성도를 높일 수 있는 것으로 나타났다. 각운동량 요인에서 기술수행 시 전체적으로 좌우(X)축에 대한 각운동량이 전후(Y)축과 수직(Z)축에 대한 각운동량에 비해 더 큰 값을 보였다. 좌우 축 각운동량은 지면에서 이지되어 동작을 수행하는 핸드스프링회전과 앞공중1회 비틀기에서 숙련자가 상대적으로 더 큰 각운동량 보이는 것으로 나타났다. The purpose of this study were a two-fold: ⑴ to analyze event and phase of handspring salto forward stretched with turn; ⑵ to know the differences in the kinematic variables between two groups. A Kwon3D program served for the estimation of this study. The group was divided into three National representative and three well-trained calisthenics in this study. The results of this study revealed that ⑴ the forward somersault performance was increased when duration time in the air was long during the salto forward stretched that the duration time of Handspring is short, the pressure at takeoff is high, the stride is large, and hands are supporting on the ground quickly; ⑵ comparing the angular movement of anterior and posterior y axis and vertical z axis, the angular movement of right and left x axis was higher during the performance. As a result, the national representative players showed better performance in Handspring salto forward stretched with turn.

미들박스 링크 비용을 고려한 라우팅 비용 변화에 대한 연구

권수경(Su-Kyung Kwon),김태준(Tae-June Kim),김정구(Jeong-Goo Kim),최윤호(Yoon-Ho Choi) 한국통신학회 2015 한국통신학회 학술대회논문집 Vol.2015 No.06

현행 중등학교 과학 실험 , 실습 교육 실태 조사 및 그 운영 진단 (Ⅱ) : 고등학교 과학 실험 , 실습 교육을 중심으로

김영호,이윤종,정원우,강용희,양승영,윤성효,안병호,윤일희,권용주,임성규,오철한,기우항,전명남,김중욱 한국과학교육학회 1998 한국과학교육학회지 Vol.18 No.3

본 연구는 96년도에 실시한 우리 나라 중학교 과학 실험·실습 교육의 실태 조사 연구에 따른 계속 연구의 일환으로 고등학교 과학 실험·실습 교육의 실태를 조사하기 위한 것이다. 연구내용은 고등학교 과학 교과서의 분석, 실험·실습 실시 현황 분석, 현행 실험·실습 교육의 문제점 분석, 현행 학교 구성원들의 실험·실습 교육 운영 실태 조사 및 현행 실험·실습 교육의 개선을 위한 연구과제 도출을 주요 연구내용으로 다루었다. 연구방법은 전국의 고등학교 80개교를 대상으로 이들 학교의 학생 1,977명, 교사 165명, 학교장 80명에게 적용하였다. 본 연구에서 밝혀진 결과를 중심으로 우리 나라 고등학교 과학 실험 실습 교육의 정상화를 위한 실험 내적인 연구과제를 제시하면 다음과 같다. (1)교재 개발에 대한 연구, (2)실험 킷트 개발에 대한 연구, (3)교사교육 및 재교육 프로그램 개발에 대한 연구, (4)교실 밖 실험·실습 활동 프로그램 개발에 대한 연구, (5)과학 실험·실습 소재의 개발에 대한 연구. This study was accomplished to analysis and survey on the experimental and practical science education of high school in korea for the consecutive study of the an analysis and survey on the experimental and practical science education of middle school in korea(Lee, Yoon-Jong et al., 1997). The status of facilities, management for the experiment, practices, teaching methods in high schools have been investigated. The present status and reasonable management of the high school science education have been grasped from the questionaires. To do this 165 high school science teachers, 1977 students and 80 principals of high schools in Korea are administered questionaires of Science Education Research Institute of Kyungpook National University(1997). The results of this study are as follows : The reasonable management for experiments and practices of science education were scanty in the high school around the urban and rural school owing to the shortage of facilities and equipments, crowded class, excessive class works for teacher, excessive contents of present textbooks and insufficiency of the administrative supports etc. The current teaching method of high school science has emphasized knowledge. This fact does not satisfy the objective of learning due to lack of the teaching method. Desirable directions for the improvement of present status of high school science education were proposed in this paper.

관상동맥 스텐트 내 재협착의 발생에 미치는 임상적 관동맥 조영술적 요인들

윤혁준,김기식,박형섭,이영수,현대우,한성욱,허승호,김윤년,김권배,박남희,원경숙 대한순환기학회 2003 Korean Circulation Journal Vol.33 No.12

六君子湯이 Cyclophosphamide로 損傷된 脾臟組織의 恢復에 미치는 影響

權五成,姜允皓 동국대학교 한의학연구소 1993 東國韓醫學硏究所論文集 Vol.2 No.1

The purpose of this study is to invesigate the effect of Yook- Gun- Ja-Tang on the side effect of cyclophosphamide to splenical tissue in the rat. The experimental animal were divided into normal group, control group, sample group by way of method treatment of the drug. Each group was sacrificed and stained in accordance with the schedule and observed under light microscope. The results of this study were as follow : 1. After treatment of Yuok-Gun- Ja-Tang, rat's weight and volume were more increased than normal group and control group. 2. The decrease of the numbers of the splenical tissue after administration of cyclophosphamide were recovered with prescription of the Yook- Gun- Ja-Tang : The decreases of white pulp, red pulp, marginal zone, central artery were recovered. 3. Increased macrophages in red pulp of splenical tissue of rats with administration of cyclophosphamide were decreased after treatment of Yook-Gun-Ja-Tang. These results appeared to suggest that Yook-Gun-Ja-Tang might be effective on the side effect of cyclophosphamide to splenical tissue of rat's and applied to the prescription for the recovery of the side effect of drug.

탈석회화골분진과 폴리에틸렌을 이용한 대용골에 관한 실험적연구

권성택,이윤호,김진환 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.6

Advances in craniofacial surgery have stimulated the interest in physiology of osseous transplantation. Numerous works have been made to understand physiology of bone graft. In the bone graft, many researches have been focused to solve the following problems; First, the shortage of donor site. Second, variable degree of resorption. Third, limitation of shaping of bone graft. This study was undertaken to evaluate the morphogenic properties of bone substitute and its fate after 6 months of implantation to the back of experimental animal. Bone substitutes were prepared by filling of demineralized bone power to the inner space of porous polyethylene block. Size of the porous polyethylene block was 10 ×10 ×7mm and that of inner space was 7 × 7 × 4mm. Experimental group consisted of implantation of bone substitute into the musculatoure of back of adult New Zealand White rabbits. Control groups were divided into 2 groups : namely group 2 which has inner space of porous polyethylene block without demineralized bone powder and group 3 which has neither innerspace nor demineralized bone powder but only porous polyethylene block. Rib bone between each implant was considered as an another group (group 4) for a reference of isotope uptake. ????Tc-MDP uptake and histomarphometric evaluation with light microscope showed induced osteogenesis of demineralized bone powder within the inside of porous polyethylene. At 1 month after implantation, composites didn't make significant difference from control groups. From 2 months to 6 months, radioisotope uptake increased with sharp curve(1.69 at 2 months, 3.35 at 4 monts and 4.53 at 6 months) and number of osteocytes, osteoblasts, chondrocytes and chondroblast also increased with steep line(3.3 at Zmonths, 27.6 at 4months, 95.4 at 6 months), while control groups could not show any evidences of induced osteogenesis and better incorporartion. The results of this study suggested that creation of osteogenic composites of porous materials and osteoinductive bone powder can be one of the rational approach to solve the problems of bone graft.

유용성 도료와 수용성 도료의 유해성 비교에 관한 연구 : 자동차 보수용 도료를 중심으로

권은혜,김광식,오정룡,최정근,정윤석,이유진,김은아,송세욱,정호근 한국산업위생학회 2001 한국산업보건학회지 Vol.11 No.1

The purpose of this study is to substitute water-based painting materials for the current solvent-based ones used in motor-repairing process to minimize the exposure of organic solvents to the painters. This study assessed the exposure of organic solvents to the painters using water-based and solvent-based painting materials and compared compositions, painting processes and the health hazards of the application of these alternative painting materials. The results of this study are as follows. 1. solvent-based painting materials used in motor-repairing process consist of various organic solvents, which consist primarily of toluene, xylene, ethyl benzene, ethyl methyl bezene, trimethyl bezene, ethyl acetate, butyl acetate, methyl isobutyl ketone, 2-ethoxy ethanol, 2-ethoxy ethyl acetate and toluene-2.4-diisocianate and the others. These organic solvents are know as health-hazardous substances. But water-based painting materials are high-solid, low-solvent ones and consist of such two organic solvents as 2-butoxy ethanol and 2-heptanone and the others. 2. The painters using solvent-based painting materials in motor-repairing process are exposed to various organic acetate, methyl isobutyl ketone, trimethyl benzene, 2-ethoxy ethanol, and 2-ethoxy ethyl acetate. But the painters using solvent-based ones are only exposed to 2-butoxy ethanol and 2-heptanone. 3. By using water-based painting materials in stead of solvent-based painting materials containing health-hazardous organic solvents, the exposure of such organic solvents in the painter's breathing zone can be largely prevented. 4. This study recommends water-based painting materials as substitutes for the current solvent-based ones used in motor-repairing process to minimize the exposure of organic solvents to the painters.

비피리딘과 금속이온을 이용한 기능성 포접체의 생성에 관한 연구

권윤정 건국대학교 산업기술연구원 2000 건국기술연구논문지 Vol.25 No.-

The crystal structure of the title complexes was determined by X-ray diffraction measurements. Tightly packed porphyrin molecules stack to form arrays of parallel channels in which a remarkable variety of guest species are accommodated. Construction of inner cavities in the solid composed mainly of organic compoments is attractive since function of the inner cavity become designable. This paper reports two kinds of complexes composed of metallic ion and bipyridine(bpy) when Cd2+ was treated with bay in different composition of solvents. Identification of the features of the molecular structure responsible for the versatility of tetraarylporphyrin-based clathrate lays the groundwork for the development of new microporous materials.

제지 폐수의 질소·인산 동시 제거를 위한 생물학적 처리기술 개발

권기석,노윤숙 안동대학교 환경연구소 2001 환경연구 Vol.1 No.-

주변에서 원유로 오염된 토양과 난분해성 화합물을 포함하고 있는 폐수 등에서 균주를 분리하여 40여종의 분해균주 중 가장 분해력이 우수한 분해균주 4종을 LB(Luria Bertani)배지로 최종분리하였다. 자연계의 균원 시료로부터 분리된 균주 중 점성을 갖는 균주를 선별하여 그 중 표준 점토광물인 kaolin clay에 대한 응집활성이 뛰어난 34종류이 균주를 선택하였으며, 폐수 내에서 응집활성이 높은 응집균주 4종을 최종 분리하였다. 균주 #46(Bacillus circulans), O24(Pseudomonas sp.)2종을 제외한 나머지 균주들의 동정결과는 #24T3, #24P3, #24B3는 Bacillus sp.로 동정되었고 #81, #152, 29은 미동정 되었다. 분해균주의 경우 TOC가 84%, T-N이 98%, T-P가 68%의 처리효율을 보였다. 응집균주의 경우 TOC가 80%, T-N 98%, T-P가 71%의 처리효율을 보였다. 분해+응집균주가 microcosom을 형성한 경우 TOC가 85%, T-N의 경우 98%이상, T-P의 경우 70%을 동시에 처리할 수 있다. From the enrichment culture, 40 strains were isolated, Among them, four strains were screend as having significant biodegradation activity of crude oil and toxic compounds containing wastewater. Also, for the screening of the microorganims, 34 bacterial strains were selected. Among them, four strains were screened as producing the most excellent flocculating material. The strains were identified that 46 was Bacillus circulates, 024 was Pseudomonas sp., 24T3, 24B3, 24P3 were identified Bacillus sp., respectively, And 81, 151, 29 were non-identification. The optimum cultural conditions were as follows; Bioflocculant producing strains : Glucose 20.0g, NH_(4)NO_(3) 2.0g, K_(2)HPO_(4) 0.8g, KH_(2)PO_(4) 0.6g, MgSO_(4)·7H_(2)O 0.05g, MnSO_(4)·4H_(2)O 0.05g, Soytone 0.lg, Tryptone 0.lg Yeast extract 0.lg, CaCO_(3) 0.3g in 1 liter of distilled water, 30℃ for temperature and pH 7.0. Biodegrade strains : LB(Luria Bertani) medium, 30℃ for temperature and pH 6.8∼7.5. The removal rates of total nitrogen and total phosphate by bioflocculant producing strains against pulp wastewater were T-N 98% and T-P 71%, respectively. In the case of biodegrade strains shown that the removal rates were T-N 98% and T-P 68%, respectively. In the case of mixed biodegrade strains and bioflocculant producing strains shown that the removal rates were T-N 99% and T-P 76%, respectively. The strains will be apply simultaneously T-N 98% and T-P 70% from pulp waserwater.