複合 韓藥劑 淸籬滋坎湯의 抗癌效果 및 作用機轉 糾明

Cho, Kyung-Sam,Kim, Si-Young,Park, Jai-Kyung,Choi, Seung-Hoon,Chung, Se-Young,Yoon, Hwi-Joong 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung Sam Cho¹Si Young Kim¹, Jai Kyung Park²,Seung Hoon Choi³,Se Young Chung⁴, Hwi Joong Yoon¹¹College of Medicine, ²East-West Medical Research Institute, ³College of Oriental Medicine, ⁴College of Pharmacology, Kyung Hee University,Seoul, Korea. The Anti-cancer Effect of Oriental Medicine Chung-Ri-Ja-Gam-Tang in the Mouse with Metastatic Lung Cancer. Proceedings of International Symposium on East-West Medicine, Seoul. 231-243, 1999.-The oriental medicine Chung-Ri-Ja-Gam-Tang is an herbal medicine which has been used for pulmonary diseases and known as having immune stimulatory effects. It has been known effective in lung cancer. So we studied the effects and the mechanisms of this herbal medicine it the mouse with metastatic lung cancer. The metastatic lung cancer of the mouse was produced with melnoma cell line(B16BL/6).5×10□/mouse tumor cells were injected intravenously to the CDF1 mouse via tail vein. The mice were divided 4 groups. The first group was treated with 50mg/kg extract of Chung-Ri-Ja-Gam-Tang per oral for 10 days after cancer cell injection, second group treated with saline after cancer cell injection, the third group with medicine without cancer cell, and the last group with saline only. After 10 days treatments some of the mice were scarificed and the lung and spleen was removed. The survival duration, weight change, the number of metastatic cancer nodule of the lung, the NK cell activity, the capacity of cytokines(INF-γ,INF-α,IL-2) production and the proliferation activities of mouse lymphocytes were measured. The surival times of the group 1 mice were longer and the weight loss was less than the group 2 significantly. The number of the metastatic nodule of the lung were decresed in the group 1 than the group 2. The production of INF-γ,was increase in group 2 than group 3 and 4, IL-2 production was increased in group 1 than group 2,3,4 significantly. There was no difference in TNF- α production and proliferation activity of lymphocyte in each group. The NK cell activity was significantly increased in group 1 than group 2,3,4. We conclude that the Chung-Ri-Ja-Gam-Tang has the effect of increasing the NK cell activity of the CDF1 mouse with metastatic lung cancer(B16BL/6). And it is suggested that the increased production of the IL-2 is the mechanisms of enhanced NK cell activity.

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

송곳니구름버섯(Irpex zonatus) BN2에 의한 다핵방향족 탄화수소화합물의 분해

최양순,윤경하 순천향대학교 기초과학연구소 1996 순천향자연과학연구 논문집 Vol.2 No.2

우리 나라 자연 환경에서 분리 동정된 송곳니구름버섯 (Irpex zonatus) BN2 균주의 리그닌 분해효소활성과 anthracene, phenanthrene 및 benzo(a)pyrene의 분해능을 조사하였다. 송곳니구름버섯 BN2 균주는 lignin peroxidase (LiP) 와 veratryl alcohol oxidase (VAO)를 생산하지 않고 laccase와 manganese dependent peroxidase (MnP)를 생산했다. MnP는 배양 3일부터 생산되었으나 효소활성은 매우 낮았다. 반면 laccase는 배양 초부터 지속적으로 생산되었고 활성은 대단히 높았다. 균주를 PAHs 화합물에 접종하여 배양했을 때 anthracene 분해율은 배양 1주에서 74.6%, 2주에서 92.9%, 4주에서 100%의 분해율을 접종하여 나타났다. phenanthrene 의 경우 배양 1주와 2주에서 각각 74.3%와 91.6%의 분해율을 나타냈고 4주에서 100%의 분해율을 나타냈다. benzo(a)pyrene 은 각각 41.8%, 49.0% 및 82.5%의 분해율을 나타내서 다른 PAHs 화합물보다 비교적 낮은 분해율을 보였다. 본 실험 결과 송곳니구름버섯 BN2 균주에 의한 PAHs 화합물의 분해는 주로 laccase에 의하여 이루어진다고 생각된다. The present research was undertaken to investigate the activity of ligninolytic enzymes and the biodegradation capability of polycyclic aromatic hydrocarbons (PAHs) with Irpex zonatus BN2, isolated from nature and identified. For the assay of enzyme activities, the isolate did not produce lignin peroxidase (LiP) and veratryl alcohol oxidase (VAO), but laccase and manganese dependent peroxidase (MnP). While the activity for MnP was low (61.6 n㏖/㎎·protein). its laccase activity was very high (1185.9 n㏖/㎎·protein). Moreover. laccase had appeared earlier than MnP When the isolate was incubated with 10㎎/L (final concentration) of anthracene, phenanthrene and benzo(a)pyrene, the isolate removed anthracene 74.6%, 92.9%, and 100% respectively for 7,14 and 28 days incubation. In case of phenanthrene was 74.3%, 91.6% and 100% respectively. Finally the results of benzo(a)pyrene was 41.8%, 49.0% and 82.5%, respectively. The results suggest that laccase of Irpex zonatus BN2 should play an important role in the biodegradation of these PAHs compounds.

서울지역 복지회관의 결식아동의 영양적 건강상태

최윤정;박유신;장유경 한양대학교 2004 韓國 生活 科學 硏究 Vol.- No.24

This study was carried out to investigated the growth development and nutritional health of poorly-fed children in Seoul Welfare Center. All subjects underwent a structured interview for general characteristics, anthropometric measurement and nutritional status. The results are as follows : The subjects were 30(male : 14, female : 16), among 7 to 12 years old. In higher grade boy, physical growth and development were decreased. Among the subjects, 37.7% were skipped the breakfast. In food group frequencies, subjects had animal protein such as meats(82.1%), fish(58.6%), and vegetable protein such as legumes(73.3%) 2 times and less a week. The average intakes of energy, vitamin B2, calcium were below 75% of the recommended dietary allowance. More than half of the subjects were under-nutrition (deficient of RDA, below 75%) in energy, vitamin A, vitamin B2, niacin, calcium and iron. Consequently, for balanced growth and development, it is necessary for school or community based nutritional support to supply dairy products that are major source of vitamin B2 and calcium for poorly-fed children.

한국인 제2형 당뇨병 환자의 골격근에서 인슐린 신호전달체계의 결함

최준혁,이관우,김효정,이동훈,이종우,김정은,엄현채,김경미,최성이,정윤석,김현만 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.5

연구배경: 제2형 당뇨병에서 나타나는 인슐린 저항성은 간, 지방, 근육 같은 말초조직에서 인슐린의 작용이 떨어지는 것을 말한다. 제2형 당뇨병의 발생기전에 인슐린 저항성과 인슐린 분비능 저하가 같이 관여함은 잘 알려진 사실이다. 인슐린의 세포내에서의 저항성을 규명하기 위한 인슐린의 세포내 신호전달체계에 대한 연구가 활발히 진행되고 있으며 몇몇 연구에서 인슐린 저항성을 가진 골격근육에서 IRS와 관련된 PI3-kinase의 활성감소와 Akt kinase의 활성감소를 보고하고 있으나 아직까지 명확하게 그 기작이 설명되어지지 않고 있다. 본 연구에서는 정상성인, 제2형 당뇨병 환자를 대상으로 경구당부하검사 및 인슐린 클램프검사를 시행하고 인슐린 클램프 검사시 대상인의 근육을 채취하여 인슐린 신호전달 체계(IR-β, IRS, Akt(PKB, Rac) kinase, GSK-3)를 연구하였다. 방법: 연구대상자는 한국인으로서 경구당부하검사상 정상인 및 당뇨병 환자 각각 11명, 9명을 대상으로 하였으며, 정상인은 건강인으로 과거력상 당대사에 영향을 줄 질환이 없고, 현재 당대사에 영향을 줄 약물 복용 및 다른 소견이 없는 경구당부하검사상 정성 내당능을 보이는 대상자로 하였다. 당뇨병환자는 모두 제2형 당뇨병 환자로 이환기간이 만 5년 이내인 경우로 하고, 인슐린으로 치료하는 대상자는 제외하도록 하였다. 대상자의 연령, 체질량지수, 체지방량, 공복시 총콜레스테롤, 중성지방, 고밀도지단백 콜레스테롤 및 HbA1c, 인슐린, C-peptide를 측정하였고, "HOMA model"을 이용하여 베타세포의 기능와 인슐린저항성 정도를 평가하였다. 정상혈당클램프 검사(euhlycemic hyperinsulinemic clamp test)를 시행하고 인슐린 투입 전과 인슐린 투입 30분후, 두차례에 걸쳐 대퇴부 근육생검을 실시한후 western 법으로 IR-β, IRS, Akt kinase, GSK-3의 인산화량을 측정하였다. 결과: 정상인 11명과 당뇨병 환자 9명을 대상으로 하였으며 대상자의 평균 연령, 평균 체질량지수, 체지방량, 허리/엉덩이 둘레의 비(waist hip ratio; WHR)는 두 군간에 차이가 없었다. 평균 공복혈당은 정상군 98.0±0.3㎎/dL, 당뇨병군 208.1±16.5㎎/dL(p<0.05), HbA1c는 정상군 5.4±0.5%, 당뇨병군 9.2±0.6%(p<0.05)였다. "HOMA model"을 이용한 베타세포의 기능과 인슐린저항성은 정상군 56.4±8.5%, 1.4±0.2, 당뇨병군 72.2±52.3%(p<0.01), 10.2±6.3(p<0.01)였으며 정상혈당클램프 검사상 포도당 이용률은 정상군 8.2±0.6㎎/㎏/min, 당뇨병군 3.7±1.1㎎/㎏/min(p<0.01)로 정상군과 당뇨병군간에 유의한 차이를 나타내었다. Western blot법으로 IR-β, IRS, Akt kinase, GSK-3의 인산화량을 인슐린 투입전과 인슐린 투입 30분후 측정하였을 때 IR-β에서는 정상군이 103.9±2.3에서 241.3±18.6, 당뇨군이 108.9±2.2에서 198.7±6.3으로 증가하였다(p=NS). 이는 인슐린 투입후 당뇨병군이 정상군과 비교하여 인산화량의 증가정도가 통계적인 차이가 없었다. IRS에서는 정상군이 111.6±7.3에서 295.6±17.2, 당뇨군이 114.5±6.1에서 222.0±23.2로 증가하였으며(p<0.05), 인슐린 투입후 당뇨병군이 정상군과 비교하여 인산화량의 증가정도가 약 24% 감소하였다. Akt kinase에서는 103.4±6.0에서 416.8±29.5로 증가하였으며(p<0.01), 인슐린 투입후 당뇨병군이 정상군과 비교하여 인산화량의 증가정도가 약 43% 감소하였다. GSK-3에서는 정상군이 107.7±6.7에서 595.7±28.1, 당뇨군이 104.3±4.8에서 443.3±12.9로 증가하였으며(p<0.01), 인슐린 투입후 당뇨병군이 정상군과 비교하여 인산화량의 증가정도가 약 25% 감소하였다. 결론: 제2형 당뇨병 환자에서 정상인과 비교하여 인슐린 자극후 IR-β는 인산화량의 증가정도가 큰 차이가 없었으나, IRS, Akt kinase, GSK-3에서는 인산화량의 증가 정도가 감소되는 것으로 보아 상위 인슐린 신호전단체계부터 결함이 있는 것으로 생각된다. Background: The glucose uptake rate is the limiting step in glucose utilization and storage. The failure of insulin to stimulate glucose uptake in muscle appears to be a primary defect of insulin resistance. This study was undertaken to examine the effect of physiological hyperinsulinemia on the phosphorylation of the insulin receptor (IR-β), insulin receptor substrate (IRS), Akt kinase and GSK-3 in isolated skeletal muscle, in people with type 2 diabetes(n=9) and control subjects(n=11). Methods: 75g OGTT and euglycemic hyperinsulinemic clamp test were done. And vastus lateralis muscle was obtained before and 30 min into the euglycemic clamp. Western blots were performed for tyrosine phosphroylation of insulin receptor substrate (IRS) and phosphorylation of the insulin receptor(IR-β), Akt and GSK-3. Result: There were no statistical differences in the mean age, BMI and body fat between the control subjects and diabetic patients. The fasting blood sugar and HbA_1c in controls and diabetic patients were 98.0±0.3 and 208.1±16.5ng/dl, and 5.4±0.5 and 9.2±0.6%, and 1.4±0.2 in the control subjects, and 72.2±52.3%(p<0.01) and 10.2±6.3(p<0.01) in the diabetic patients, respectively. The insulin resistance from the euglycemic hyperinsulinemic clamp test were 8.2±0.6㎎/㎏/min and 3.7±1.1ng/㎏/min in the control subjects and in the diabetic patients, respectively(p<0.01). Compared with the normal controls, insulin-stimulated IR phosphorylation was no different to that in the diabetic patients. However, insulin-stimulated IRS phosphorylation, insulin-stimulated Akt phosphorylation and insulin-stimulated GSK-3 phosphorylation were reduced in the diabetic patients compared with the normal controls by 24, 43 and 25%, respectively(p<0.05). Conclusion: In Korean type 2 diabetic patients, the insulin resistance may be due to the impairment of the upstream insulin signal molecular network. Further studies will focus on determining whether these signaling defects are the cause of the development of insulin resistance, or secondary to the altered metabolic state, associated with type 2 diabetes mellitus (J Kor Soc Endocrinol 17:685∼697, 2002).

디지털 시대의 e-마케팅 전략에 관한 연구

최윤홍,박경아 호남대학교 2005 호남대학교 학술논문집 Vol.26 No.1

This study provides marketing framework about how to operate strategic e-business successfully under rapidly changing environment. While considering the ultimate goal of e-business as profit securing through producing customer value, this study suggests future e-marketing strategy focusing in understanding and utilizing opportunities arising under rapidly changing environment. First, e-business should be customer-focused. One should escape from product-focused mear-sightedness to thinking about customer's desire and dream. Second, e-business should be future inclined. One should escape from the management that focuses too much on the existing market or present to the direction where one can utilize the future opportunity. Third, e-business should manage online and offline comprehensively so that they can produce synergy effect. Successful operation of e-business requires comprehesive and efficient utilization of value chain that online and offline are connected. Moreover, e-marketing process closely interacts with each other. It is difficult to establish effective e-business mission or model without ideas in opportunity analysis or STP strategy. Therefore, effectiveness of e-marketing process requires close interation between the processes.

Citric Acid의 分光光度定量法에 關한 硏究

崔閏壽,趙庚烈 대구효성가톨릭대학교 1984 연구논문집 Vol.28 No.2

A spectrophotometic determination of citric acid was studied using dicychohexylcarbodiimide (D.C.C) as a reagent. Citric acid was reacted with D.C.C to form active O-acyl derivative which gradually changed, yellowish coloured, inactive N-acyl derivative. Optimum conditions for quantitative analysis were investigated spectrophotometrically and the structure of N-acyl derivative was estimated by IR, NMR spectra and cryoscopic method. Molar ratio(1:3) and M.W. (803) were measured. Since N-acyl derivative was readiliy hydrolyzed in hydrous solvent, the acylation must be proceeded in the anhydrous solvent, N,N-dimethylformamide(D.M.F). The linear calibration curve of citric acid were obtained in the range from 420 to 480 ㎍/ml with λ? 430 nm (ε=2040) and from 9 to 25 ㎍/ml with λ? 290nm (ε=25680). A characteristic feature of this method is that citric acid can be determined even in presense of various carboxylic acid and amino acid.

항문 주위 피부로 전이한 위암 1례

최경현,윤기영,이상호 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Gastric adenocarcinoma can metastasize to various organ sites such as regional and distant lymph node(s), peritoneum, liver, ovary, lung, brain, bone, adrenal gland, skin and other sites via lymphatics and blood flow, or by direct seeding. But isolated perianal metastasis without other skin area involvement was rare and we could not find in the literature survey. So we are going to report this case; Patient was 48 year-old male with Borrmann type III adenocarcinoma(tubular poor type) located lesser curvature side of the Upper and Middle stomach invaded to underlying pancreas. He received total gastrectomy and Roux en Y gastrojejunostomy, distal pancreatectomy and splenctomy at Feb 26th 1999. Four out of 33 regional nodes were metastatic. He recovered farely well after operation and resumed work as full time farmer until Jul 11th 2003 follow up exams when he was diagnosed as multiple liver metastases. Tumor markers was as follow; CEA 5.2 ng/ml(he smokes a pack per day), CA 19-9 11.5 U/ml and urine pepsinogen 30.6 ng/ml(the pre-hepatic recurrence level was 8.1 ng/ml). He received 7 cycles of taxotere and cisplatin intravenous chemotherapy with complete remission on CT scan at Nov 18th 2003. Then he developed a metastatic perianal adenocarcinoma at May 4th 2004. It did not respond to radiation therapy. So it was completely resected out with two resection procedures and an end type sigmoid colostomy for fecal diversion. There are no recurrence until Jul 24th 2007 when he received IM injection of vitamin B12 and follow up exams. The tumor markers was normal limit including urine pepsinogen 11 g/ml. With this experience, all patients should be carefully followed up after gastric cancer operation for recurrence. Urine pepsinogen level can be a useful tumor marker after total gastrectomy for gastric adenocarcinoma. Don't give up patient with recurrences before various efforts to eradicate it.

이중보온 온수배관의 부식피로균열 특성에 관한 연구

최윤석,김정구,정민경,이훈 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.2

The corrosion fatigue properties of thermally insulated pipeline were investigated in synthetic groundwater by electrochemical test, corrosion fatigue test and SEM analysis. Since the potential difference between the weldment and the base metal was small, the pipeline steel was not susceptible to galvanic corrosion. No fatigue limit was apparent for corrosion fatigue testing with remarkable reduction of fatigue lifetime. The effects of insulation and stress on the corrosion fatigue resistance of pipeline steel were studied through linear polarization resistance (LPR) and electrochemical impedance spectroscopy (EIS) during corrosion fatigue testing. The result of LPR measurement indicated that the corrosion rate was determined not by the water content of PUR foam but by the magnitude of applied stress. The better corrosion fatigue property of the insulated steel was attributable to the formation of a protective rust layer under the insulation, which reduced the acceleration of corrosion process and the propagation of fatigue crack. This was confirmed by the results of EIS measurements and SEM observation.

Ascorbic Acid의 分光光度 定量法에 關한 硏究(第一報)

崔閏壽,趙庚烈 대구효성가톨릭대학교 1981 연구논문집 Vol.23 No.3

A new spectroscopic method was established for the determination of ascorbic acid with indole as a reagent. Indole was reacted with ascorbic acid to form complex compound if the presence of Cu(Ⅱ) ion. The maximum absorbance of the complex compound was at 480nm. Various conditions, (eg, PH, amounts of reagents, temperature of reactants and standing time) were investigated for the maximum colour intensity. Calibration curve for ascorbic acid was linear over a sample of each ascorbic acid in the range of 100㎍/ml. Extinction coefficient was measured, ε=12,592.