형광검출 역상 액체크로마토그래피에 의한 Histamine의 정량

유희춘,김형룡,김상현,김대기,이영미,김형민,안년형,신태용 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

Histamine을 정확하고 신속하게 정량하기 위해 9.Fiuorenylmethyl chloroformate를 형광유도체화제로 하여 역상 HPLC법으로 정량하였다. 히스타민을 형광유도체화할 때 반응액의 pH, 반응시간, 형광유도체화제의 농도 등 최적 반응 조건을 검토하였다. 이 방법으로 히스타민을 분석한 결과 0.1~0.5ug/ml의 농도범위에서 상관계수가 0.922인 양호한 직선성을 나타내었으며 검출한계는 0.01??/ml였다. A rapid and simple method for the determination of histamine by reverse-phase high performance liquid chromatography with fluorescence detection was established. 9-Fluorenylmethyl chloroformate(FMOC) was used as fluorescent derivative reagent. The optimum conditions for the derivatiation such as pH, reaction time and he concentration of FMOC were investigated. Linearity of calibration curve was obtained between 0.1ug/ml/and 0.5ug/ml(r=0.922) and the limit of detection was 0.01ug/ml.

거대 난소 종양의 염전 1예

김미영,이해혁,유애리,박정희,이임순,정수호,고은석,레앙소팥,배동한,김태희,이권해 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.1

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겔여과크로마토그라피에 의한 질트리코모나스의 항원분획 분석

임미혜,심재찬,민득영,안명희,류재숙,최영길 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

This study was aimed to analyze the antigenicity of T. vaginalis using a high performance gel filtration chormatography(HPLC), enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunoelectotransfer blot (EITB). Soluble antigen was prepared using a bead beater. Antiserum was collected from rabbit immunized previously. The results obtained are as follows: the crude antigen of T.vaginalis was separated into 7 peak by HPLC and the fraction 3 revealed the highest reactivity in DLISA. The immunogenic bands of fraction 3 were visualized at 38, 45, 47, 55, 64, 78, 94, 115, 126 and 180 KDa and the bands at 38KDa and 180KDa showed stronger reactivity than that of equivalents of the crude antigen in EITB. In conclusion the fraction 3 of the crude antigen of T. vaginalis obtained by chromatographic separation may have a high antigenicity and proteins ay 38KDa and 180KDa of the fraction 3 may play an important role in antigen-antibody reaction in T. vaginalis infection.

절편모충(Trichomonas vaginalis)에 대한 단일클론항체의 생산과 그 특성

임미혜,류재숙,안명희,최영길,민득영 한양대학교 의과대학 1999 한양의대 학술지 Vol.19 No.1

Trichomonas vaginalis, mucosal protozoan parasite of the urogenital tract, is responsible for the most common clinically recognized sexually transmitted disease. Surface antigen of parasitic protozoa play the important roles in disease pathogenesis by serving as attachment factors, direct virulence factors, or mediating evasion of the host immune response through direct suppressoin or antigenic variation. In this study seven monoclonal antibodies against plasma membrane of T. vaginalis HY-1 were prepared and were investigated the effector function of them. Seven monoclonal antibodies were produced and their isotypes were Ig G1 (Tv1, Tv7), Ig G2a (Tv4), Ig M(Tv2, Tv3, Tv5, Tv6). All of the seven monoclonal antibodies recognized the determinants located on the surface of live organism by IFAT. Although these surface reactive seven monoclonal antibodies agglutinated T. vaginalis trophozoites, especially MAb Tv1 and Tv4 could agglutinate eight isolates of T. vaginalis. In immunoblot assay, MAb Tv1 & Tv4 detected single peptide with molecular weight of 60 kDa, and Tv7 detected 80 kDa. On the other hand Tv2 and Tv6 detected two (40 kDa & 120 kDa) or more peptides (62 kDa, 64 kDa & 120 kDa), and Tv3 and Tv5 did not bind any pepetide. From these results, MAb Tv1 reacted with 60 kDa, major surface membrane protein of T. vaginalis and agglutinated trichomonads.

(R)과 (S)형의 N-(4-{3-[1-(4-tert-Butylpheny)ethyl] thioureidomethyl}-phenyl)-methanesulfonamide 의 합성 및 바닐로이드 수용체 길항효과

장미정,유정현,정정화,김희두 숙명여자대학교 약학연구소 2003 약학논문집-숙명여자대학교 Vol.19 No.-

(R) & (S)-form of N-(4-{3-[l-(4-tert-Butylphenyl)ethyl]thioureidomethyl} phenyl)methanesulfonamide was designed, synthesized and evaluated as a vanilloid receptor antagonist.

일부 임상간호사들의 직무스트레스와 우울과의 관련성

이미옥,류소연,변익건,박정희 朝鮮大學校 附設 醫學硏究所 2004 The Medical Journal of Chosun University Vol.29 No.2

Objective : This study was performed to identify the relationship between job stress and depression of nurses who work at hospitals in Gwangju city. Methods : The data were collected from October 2 to 22, 2003 using the self-administered questionnaire which was composed demographic characteristics, job-related factors, health-related factors, family-related factors, job stress, self-esteem and Korean Beck's depression inventory. The statistic methods were t-test, analysis of variance (ANOVA), Pearson's correlation analysis and multiple regression analysis. Results : 1. The score of job stress of clinical nurses was 169.23 (24.44) , and depression point was 11.37 (7.71). By the depression criteria, the prevalence of depression were 11.2% in pre-depression, 5.7% in moderate and 4.6% in severe depression. 2. It was found that the characteristics that had a statistically significant relation with depression scores were age, marital status, educational background, career, income, drinking status, satisfaction in living and self-esteem. And job stress was statistically significant positive correlation with depression scores. 3. As a result of multiple regression analysis, the job stress had not a statistically significant relation with depression with controlled other related factors. Conclusion : It is necessary to prepare the program about the control and prevention of depression In clinical nurses. Further study need to identify the relationship between depression and job-related factors in nurses working at the hospitals.

골반 초음파 감시 하에 16 Gauge Angioneedle™을 사용하여 치료적 더글라스와 천자를 시행하여 치유된 복강 내 출혈 1예

김미영,이해혁,유애리,박정희,이임순,정수호,레앙소팔,배동한,김태희,이권해 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.1

Culdocentesis is a procedure that checks for abnormal Quid in the space just behind the vagina (cul-de-sac). First, a pelvic examination is done. Then, after exposing the postehor vaginal fonix with a bivalve vaginal speculum the posterior lip of the cervix is grasped with a tenaculum. The cul-de-sac is then entered through the postehor vaginal wall with an 18-20 gauge needle with a syringe inserted. As the cul-de-sac is entered, suction is applied, and the intraperitoneal contents are aspirated. In the recent past, culdocentesis was commonly used in the evaluation of a vahety of common intraperitoneal conditions, principally, the hemorrhage associated with ectopic gestations and ruptured ovarian cysts the presence and/or drainage of purulent associated with pelvic inGammatory disease, and as a screening procedure to facilitate early diagnosis of ovarian neoplasms. Usually 16-18 gauge needle with a syhnge attached was used for culdocentesis. But we have experienced a case of therapeutic culdocentesis with 16 gauge angioneedle in patient of hemoperitoneum. So, report it with a bhef review of literature.

ASTM F2027 조직공학적 의료용제품(TEMPs)을 위한 기질의 특성결정 및 테스트를 위한 표준 지침서

백미옥 ( Mi Ock Baek ),소정원 ( Jung Won So ),김순희 ( Soon Hee Kim ),노혜원 ( Hye Won Roh ),이나리 ( Na Ri Lee ),김문석 ( Moon Suk Kim ),유규하 ( Gyu Ha Ryu2 ),조양하 ( Yang Ha Cho ),이승진 ( Seung Jin Lee ),강길선 ( Gilson Khang 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.4

Tissue engineering medical products are those protocols and products developed for use in the human body as biological substitutes to restore, maintain, or improve tissue function. The purpose of this standard is to locate relevant existing guideline and test methods and to provide guidance for interim use of materials for which a standard does not exist. The standard may be use as guideline in tissue engineered research.

ASTM F813-07 의료용디바이스를 위한 생체재료의 직접촉 세포배양측정에 대한 표준지침서

김순희 ( Soon Hee Kim ),백미옥 ( Mi Ock Baek ),소정원 ( Jung Won So ),노혜원 ( Hye Won Roh ),이나리 ( Na Ri Lee ),김문석 ( Moon Suk Kim ),유규하 ( Gyu Ha Ryu2,),조양하 ( Yang Ha Cho ),이승진 ( Seung Jin Lee ),강길선 ( Gilson Khang 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.4

We provided standard practice for direct contact cell culture evaluation of materials for medical devices in this report. This practice introduced a reference method of direct contact cell culture testing. This method of direct contact cell culture may be used in the construction of medical materials and devices for test the cytotoxic potential of materials. Researcher for medical devices could use this practice to compare other cytotoxicity tests or directly to evaluate materials. Cell or materials could be sometimes modified depending on use in offered extend of this practice.

Non-typhi Salmonella 균혈증의 임상양상과 항균제 내성빈도

이상오,이미숙,양성연,조용균,김은옥,김양수,우준희,류지소,배직현 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.1

목적 : Non-typhi Salmonella는 대부분 발열과 설사를 동반한 급성 위장관염을 일으키며 수일에서 수주후 자연 치유된 다. 그러나 때로는 항균제 치료를 요하는 균혈증을 일으키기도 한다. 이에 저자들은 non-typhi Salmonella 균혈증이 혈액 배양에서 증명된 환자들에서 연령 및 기저질환의 유무에 따른 임상양상의 차이와 내성균 발생빈도에 대해 고찰하고자 한다. 방법 : 1989년 6월 1일부터 1996년 5월 31일까지 서울중앙병원에 입원했던 non-typhi Salmonella 균혈증 환자 66예를 대상으로 후향적으로 조사하였다. 임상미생물 기록을 조사하여 연도별 각 혈청군의 분포와 항균제 내성빈도를 분석하였다. 이들 중 56예의 의무기록을 검토하여 소아와 성인, 기저질환의 유무에 따른 임상양상의 차이와 치명율을 비교하였다. 결과 : 대상 환자 56예중 남자가 30예, 여자가 26예이었고, 소아가 16예(평균 20±1.9세, 범위 21일-7세), 성인이 40예(평균 45.7±19.1세, 범위 18세-19세)이었다. 소아에서는 4예(25.0%)에서만 기저질환이 있었고 사망환자는 한 예도 없었으나 성인에서는 27예(67.5%)에서 다양한 기저질환이 있었고(p<0.01) 4예의 환자가 패혈성 쇽으로 사망하였다. 이는 성인군에서는 10%, 성인 기저질환군에서는 14.8%(p=0.06)의 치명율을 나타내는 것이었다. 균혈증의 원발부위에 따라 분류하여 보면 위장관염 기원이 총 56예 중 31예(55.4%)로 가장 많았고 소아군에서는 기저질환이 없는 12예 중 11예(91.7%), 성인군에서는 13예 중 10예(76.9%)였으며 이들 중 사망은 한 예도 없었다. 원발성 균혈증으로 나타났던 성인 19예(47.5%) 중 18예(94.7%)에서 기저질환이 있어서(p<0.01) 기저질환이 있는 성인 환자에서 주로 원발성 균혈증이 발생함을 알 수 있었다. 국소형 감염에서 기원한 균혈증은 2예(3.6%)로 각각 담도염과 담낭염에서 발현하였다. 각 혈청군별 분포는 총 66예 중 A군 11예(16.7%), B군 25예(37.9%), C₁군 3예(4.5%), C₂군 2예(3.0%), D군 24예(36.4%), E군 1예(1.5%)이었다. 1993년 이전에는 B군이 전체의 51.6%로 다수를 차지하였으나 1994년 이후로는 D군이 57.1%로 주종을 이루었다. 한 개 이상의 항균제에 내성을 보인 내성균 발생빈도는 66예 중 18예로 27.3%를 보였다. 혈청군별로는 B군이 D군에 비해 더 높은 내성율을 보였다(44.0% ; 8.3%, p<0.01). 항균제별 내성율은 ampicillin, piperacillin이 각각 15.1%, ampicillin/sulbactam, trimethoprim/sulfamethoxazole이 각각 6.0%, tetracycline, aztreonam이 각각 3.0%, ticarcillin, gentamicin이 각각 1.5%를 보였다. 그러나 3세대 cephalosporin계와 quinolone계에는 내성을 보인 예가 없었다. 결론 : Non-typhi Salmonella 균혈증은 지저질환이 없는 소아나 성인에서는 급성 위장관염의 합병증으로 일시적인 균혈증을 보일 수 있으나 양호한 예후를 보인다. 그러나 면역기능 저하를 동반하는 기저질환을 가지고 있는 환자에서는 주로 원발성 균혈증으로 나타나며 중증의 임상 경과와 사망을 초래 할 수 있다. 항균제 내성율은 전체적으로 27.%였고 3세대 cephalosporin계와 quinolone계에는 내성을 보인 예가 없었다. Objectives :Non-typhi Salmonella causes acute gastroenteritis accompanied with fever and diarrhea, but it's usually cured spontaneously several days or weeks later. Sometimes, however, it can result in bacteremia which requires antibiotic treatment. In this paper, we're going to discuss differences in clinical manifestation depending on the age and existence of the underlying diseases and incidence of the resistant strains in the patients who suffer from bacteremia with non-typhi Salmonella proved in the blood culture. Methods : We retrospectively studied 66 cases of bacteremia with nontyphi Salmonella treated at Asan Medical Center from June 1, 1989 to May 31, 1996. The clinical microbiology records were examined and the yearly distribution of each scrogroup and the rate of resistance of antibiotics were analysed. We reviewed the medical records of 56 cases and compared the differences in clinical manifestation and fatality rate among children and adults, and the existence of the underlying diseases. Results : Of 56 ones for this study, there were 30 males, 26 females, and 16 children (mean age:2.0±1.9, range:21days-7years old) and 40 adults(mean age:45.7±9.1, range:18-91years old) were involved. Only 4 cases(25.0%) of children had underlying diseases with no dead cases, however, in adults, 27 cases(67.5%) had various underlying diseases(p<0.01), and 4 cases died of softie shock. In result, the fatality rate was 10% in adults group and 14.8%(p=0.06) in adults with underlying diseases. The origin of bacteremia of 31 cases(55.4 %) was gastroenteritis. Eleven cases(92.7%) of 12 children and ten(76.9%) of 13 adults without underlying diseases were from gastroenteritis and there were no dead cases. Of the cases of aults, the primary bacteremia were 19 cases(49.5%) and 18 cases(94.7%) of them had various underlying diseases(p<0.01). So we found that the bacteremia with non-typhi Salmonella occurred mainly as the primary bacteremia in adult cases with underlying diseases. Only two cases of bacteremia were originated from localized infection and each of them manifested at cholangitis and cholecystitis. Related to the serogroup distribution of total 66 cases, there were 11 cases(16.7%) in A group, 25 cases(37.9%) in B, 3 cases(4.5%) in C₁, 2 cases(3.0%) in C₂, 24 cases(36.4%) in D, and one case(1.5%) in E group. Until 1993, B group was a majority(51.6%), but D group became a majority(57.1%) since 1994. The rate of resistance of one or more antibiotics was 27.3%(18 cases of 66 cases). B group showed higher rate of resistance than D group(44.0% vs. 8.3%, p<0.01). Related to the respective rates of resistance of antibiotics, ampicillin and piperacillin showed 15.1%, ampicillin/sulbactam and trimethoprim/sulfamethoxazole were 6.0%, tetracyclin and aztreonam were 3.0%, and ticarcillin and gentamicin were 1.5%. The third generation cephalosporins and quinotones, however, had no cases showing resistance. Conclustion : Non-typhi Salmonella can show a transient bacteremia as the complication of acute gastroenteritis to the children as well as adults without underlying diseases. In general, it can be easily cured. To the patients with underlying diseases causing immunocompromised state, however, it can be manifested to the primary bacteremia and can cause fetal disease or death. General rate of resistance of antibiotics reaches at 27.3%, but the third generation cephalosporins and quinolones had no cases showing resistance.