corrigendum

한국조직공학과재생의학회 한국조직공학과 재생의학회 2011 조직공학과 재생의학 Vol.8 No.6

발생과정에서 연골형성의 조절과 이의 연골조직공학에의 응용

김소영 ( So Young Kim ),임군일 ( Gun Il Im ) 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.4

연골은 중배엽성 기원의 조직으로서 성체에서 관절의 윤활 및 충격흡수기능을 담당한다. 관절연골은 손상 후 잘 재생이 되지 않으므로 조직공학적인 방법을 통하여 연골의 재생을 시도하려는 연구가 많이 진행되고 있다. 발생과정에서 연골이 생성되는 과정을 잘 이해한다면, 즉 연골분화과정을 조절하는 외인성 요인(환경적)및 내재성 요인(유전적 조절)에 관한 정확한 기전을 안다면 이를 연골조직공학에 적용하여 보다 성공적인 인공연골을 개발할 수 있을 것이다. 본고에서는 발생과정중의 연골분화과정 중에 발생하는 형태유전적인 변화 및 조절기작과 연골의 전사조절인자 및 연관된 유전자의 역할, 간엽 응축 및 연골의 성숙에 있어 세포외기질의 역할을 정리하고 발생과정의 연골분화를 모방할 것으로 추정되는 성체연골모전구세포에 대한 최신 지견을 요약하며 또한 연골재생에 최근에 적용되고 있는 새로운 생체재료들과 그 적용에 대해서 논의하고자 한다.

골조직공학에서의 생체재료 지지체의 적용과 혈관생성

김소영,임군일 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.14

골조직공학은 지지체를 이용한 골결손의 치유와 재생을 목적으로 생분해성 지지체에 골형성 세포 (osteogenic cells)를 이식하는 다양한 기술을 근간으로 한다. 본 총설에는 지지체의 재료 개발의 관점 과 신생혈관생성의 관점에서 골조직공학의 발전에 대한 최신지견을 소개하고자 한다. 우선, 골조직공학 의 원리 및 생체재료의 주요 구성성분 그리고 조직공학적 골형태의 지지체 제작에 사용된 새로운 재료의 개발(합성폴리머와 칼슘인산염의 혼합체 등)에 대해서 소개하고 이들 재료의 다공성, 기공의 크기, 상 호결합성, 기공벽의 세부구조 등의 평가를 통해 이들의 장점과 한계에 대하여 논의하고자 한다. 또한 실 험실의 결과를 성공적으로 임상에 적용하기 위하여는, 미세혈류(microcirculation)의 유도가 필수적인데 본고에서는 골조직공학에서의 혈관망(vascular network)의 중요성에 대하여 논의하고 최근 연구되고 있 는 혈관형성의 촉진 방법을 소개하고자 한다.

줄기세포를 이용한 조직공학적 치아재생 동향

전소영 ( So Young Chun ),이효정 ( Hyo Jung Lee ),최영애 ( Young Ae Choi ),김재영 ( Jae Young Kim ),조제열 ( Je Yeol Cho ),신홍인 ( Hong In Shin ),박의균 ( Eui Kyun Park ) 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.1

This review focuses on the current understanding of tooth regeneration using embryonic tooth budderived tissues/cells and adult stem cells, and tissue engineering technologies combined with biomaterials. Tooth development is achieved by sequential and reciprocal interactions between the oral epithelium and the mesenchyme. Especially growth factors, transcription factors and extracellular matrix proteins play key roles during tooth development process. To achieve successful bio-engineered tooth, several different sources for epithelium and mesenchymal stem cells are needed to be assessed their potential efficacy. Next, discussion focuses on cell culture and implantation methods, particularly, medium supplements, gene transfection, chimeric tooth bud and optimized implant regions. At the end, we introduce the various biomaterials designed via biomimetic strategies for tooth regeneration applications. Although currently these approaches have several limitations including abnormal morphology of bio-engineered tooth, the tissue engineering approaches for dental regeneration using stem cells is the significant challenge in dental field. Therefore, the knowledge of the stem cells as well as the biomaterials will help our understanding for the tooth regeneration.

인간배아줄기세포와 기형종: 전분화능의 증거와 조직공학 리서치 툴로서의 가치

이유진,김한수 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.14

Human embryonic stem cells (hESCs) and recently introduced induced pluripotent stem cells (iPSCs) are stem cells with an indefinite self-renewal and the potential to generate any mature cell type. Because of these properties, pluripotent stem cells hold great promise for regenerative medicine. One of the critical issues, however, is the risk of teratoma formation after transplantation of derivatives of these pluriopotent stem cells. Experimentally, transplantation of hESC and iPS cells into immunodeificient animal led to the formation of tumors composed of all three germ layers, i.e. teratoma. This property is considered the gold standard for demonstrating pluripotency of a given cell type. At the same time, the same type of assay hold promise as a standard for assessing safety of the therapeutic cells intended for clinical applications. This review article describes the key aspects of pluripotent stem cellderived teratoma formation and utilization of teratoma as a research tool for human embryonic development or disease pathogenesis. The promises and limitations of current ES cell-based therapy are also discussed. Human embryonic stem cells (hESCs) and recently introduced induced pluripotent stem cells (iPSCs) are stem cells with an indefinite self-renewal and the potential to generate any mature cell type. Because of these properties, pluripotent stem cells hold great promise for regenerative medicine. One of the critical issues, however, is the risk of teratoma formation after transplantation of derivatives of these pluriopotent stem cells. Experimentally, transplantation of hESC and iPS cells into immunodeificient animal led to the formation of tumors composed of all three germ layers, i.e. teratoma. This property is considered the gold standard for demonstrating pluripotency of a given cell type. At the same time, the same type of assay hold promise as a standard for assessing safety of the therapeutic cells intended for clinical applications. This review article describes the key aspects of pluripotent stem cellderived teratoma formation and utilization of teratoma as a research tool for human embryonic development or disease pathogenesis. The promises and limitations of current ES cell-based therapy are also discussed.

토끼 모델에서 탈미네랄화된 골분을 함유한 PLGA 지지체를 이용한 조직공학적 골 재생

장지욱 ( Ji Wook Jang ),박기숙 ( Ki Suk Park ),김순희 ( Soon Hee Kim ),박종수 ( Chong Soo Park ),김문석 ( Moon Suk Kim ),한창환 ( Chang Whan Han ),이종문 ( John M. Rhee ),강길선 ( Gil Son Khang ),이해방 ( Hai Bang Lee ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.1

Demineralized bone particle (DBP), one of the significant natural bioactive materials, has a powerful inducer of new bone growth. It has been recognized that DBP contains many kinds of osteogenic and chondrogenic cytokines as bone morphogenic protein (BMP). BMP acts as local mitogen to stimulate proliferation of mesenchymal stem cell. Bone marrow stromal cell (BMSCs) can be differentiated in culture into osteoblasts, chondrocytes, and myoblasts with controlling of the environment of cell growth. In this study, we developed the DBP loaded poly(L-lactide-co-glycolide) (PLGA) scaffolds for the possibility of the application of the tissue engineered bone. PLGA/DBP scaffolds were prepared by solvent casting/salt leaching method and characterized by porosimeter, scanning electron microscopy, and differential scanning calorimetry. BMSCs were isolated from femur of New Zealand white rabbit and cultured with osteo-medium for osteogenic differentiation. DBP impregnated PLGA scaffolds with BMSCs were implanted into the head and femur of New Zealand white rabbit to observe the effect of DBP on the osteoinduction compared with control scaffolds. Thin sections were cut from paraffin embedded tissues and histological sections were stained hematoxylin & eosin and safranin-O. It can be observed that the porosity was above 94.6% and the pore size was above 69.8 ?m. In vivo study, we could observe that similar to bone tissue region in PLGA/BMSCs and PLGA/BMSCs/DBP groups, but bone tissue region did not occur almost in control scaffolds. From these results, it seems that DBP play an important role for bone induction on BMSCs.

조직공학용 나노섬유의 제조 및 응용

김이슬,이주영,이봉,전흥재,김천호,김재호,이해방,김문석,강윤미 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.4

Abstract : The simplicity of the electrospinning process make this technique attractive for various tissue engineering applications. The nanofibers prepared by electrospining have been investigated as promising scaffolds since they mimic the nanoscale properties of native extracellular matrix. The current review presents the preparation of electrospun nanofibers by providing a brief description of the theory in the process and electrospun materials such as biodegradable, non-degradable, and natural materials. Abstract : The simplicity of the electrospinning process make this technique attractive for various tissue engineering applications. The nanofibers prepared by electrospining have been investigated as promising scaffolds since they mimic the nanoscale properties of native extracellular matrix. The current review presents the preparation of electrospun nanofibers by providing a brief description of the theory in the process and electrospun materials such as biodegradable, non-degradable, and natural materials.

인간 디스크세포와 PLGA/DBP 지지체를 이용한 생체조직공학적 바이오 디스크

고연경,김순희,하현정,김문석,한창환,손영숙,이종문,이해방,강길선 한국조직공학과 재생의학회 2007 조직공학과 재생의학 Vol.7 No.1

We fabricated poly(lactide-co-glycolide)(PLGA) scaffolds impregnated demineralized bone particle(DBP)(PLGA/DBP) to investigate the effect of cell viability, proliferation and characteristic maintenance of intervertebral disc(IVD) cells. DBP-loaded PLGA scaffolds were prepared by solvent casting/salt leaching. Human IVD cells were seeded in PLGA/DBP scaffold, and then cell viability and proliferation according to DBP content were analyzed by 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide(MTT) assay. In MTT assay results, cell viability in scaffolds impregnated 20 and 80 wt% of DBP were higher than other scaffolds. Reverse transcript polymerase chain reaction(RT-PCR) was assessed to measure mRNA expression of the disc target genes(aggrecan and type II collagen) of human IVD cells about various content of DBP. After implantation, thin sections were cut from paraffin embedded tissues and histological sections were stained H&E staining for observation of disc cell distribution. We concluded that the using of DBP(especially 20 wt% of DBP) in terms of scaffold fabrication for bio-disc with human IVD cells helps growth of disc cells maintained their phenotypes.

지방조직 재생의 조직공학적 접근법의 최근 동향 분석

이은경 ( Eun Kyung Lee ),손영숙 ( Young Sook Son ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.4

조직공학적 바이오혈관의 기술동향과 전망

김상헌 ( Sang Heon Kim ),김수현 ( Soo Hyun Kim ),김영하 ( Young Ha Kim ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.1