둥근마 에탄올 추출물의 항염증 효과

김정환ㆍ김대용(Jeung Hoan KimㆍDae Yong Kim) 한국인체미용예술학회 2016 한국인체미용예술학회지 Vol.17 No.1

Dioscorea bulbifera has been cultivated in Korea, China and Japan as a food, and also widely used as a traditional medicine for a long time. This study was conducted to determine the anti-inflammatory effect of Dioscorea bulbifera ethanol extracts. The inhibitory effect of Dioscorea bulbifera ethanol extracts were evaluated regarding production or expression of inflammatory mediators in macrophage cells. Dioscorea bulbifera ethanol extracts significantly inhibited the expression of IL-4, IFN-γ and PGE2 and expression of COX-2, iNOS, TNF-α, in LPS-stimulated RAW264.7 cells. In addition, we evaluated the inhibitory effect of Dioscorea bulbifera ethanol extracts on an atopic dermatitis-like mouse model. An atopic dermatitis-like skin lesion was induced by repeated treatment of DNCB on the ear skin of ICR mice. The efficacy of Dioscorea bulbifera ethanol extracts was tested by histophathologic analysis. Histophathologic analysis revealed a reduction in the ear thickness in Dioscorea bulbifera ethanol extracts treated mice. These results show that Dioscorea bulbifera ethanol extracts have an anti-inflammatory effect. Therefore, this research suggests that Dioscorea bulbifera may be a potential agent for ameliorating inflammation.

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

저체중 출생이 학동기 및 청소년기 소아 천식의 중증도에 미치는 영향에 대한 고찰

정훈 ( Hoon Jeung ),김형대 ( Heung Dae Kim ),이윤진 ( Yun Jin Lee ),이균우 ( Kyun Woo Lee ) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2006 소아알레르기 및 호흡기학회지 Vol.16 No.1

목 적 : 본 연구는 저출생 체중아로 출생한 소아에 있어서 학동기 및 청소년기 소아 천식의 중증도에 미치는 영향에 관해서 후향적으로 검토하였다. 방 법 : 대동병원 소아과와 호흡기내과에 통원 중에 있거나 부산 동래구 및 연제구 소재 개인의원에서 대동병원으로 전원 된 천식 환자 중에서 8세 이상 18세 이하의 소아 및 청소년을 대상으로 출생체중을 기준으로 1,500 g 이상 2,500 g 미만인 15명을 추출하여 연구군으로 하고 출생체중 2,500 g 이상이며 연구군의 나이에 일치하는 순서대로 2명씩 추출하여 이 30명을 대조군으로 하였다. 결 과 : 양군을 비교한 결과 천식 중증도는 연구군에서 유의하게 높았다.(P=0.015) 또 흡입 스테로이드 약의 사용 빈도(증례군 13명/15명, 대조군 4명/30명 : P=0.0001)와 사용 기간에 있어서도 연구군에서 유의하게 높게 나왔다.(P= 0.009) 혈중 총 IgE치와 아토피피부염의 유병률은 대조군에서 높았다.(각각 P=0.007, P=0.0001) 폐기능 검사와 PC(20), 특이 IgE 항체는 두 군 사이에 유의한 결과를 볼 수가 없었다.(P>0.05) 결 론 : 천식의 중증도와 흡입 스테로이드 사용의 빈도와 기간 등으로 미루어 볼 때 저출생 체중은 소아 천식의 중증도를 증가시키는 요인 일 수 있음을 시사한다. Purpose : Over the past several decades, the survival rates of low birth weight(LBW; birth weight <2,500 g) infants have been improving. Many of these infants have had bronchial asthma(BA) at childhood and aldolescence. The aim of this study is to investigate whether LBW influenced the severity of BA in prealdolescent children and aldolescence by a retrospective case control study. Methods : 15 LBW children(1,500 g<birth weight<2,500 g) between 8 year-old and 18 year-old with BA visiting Dae-dong hospital as the case group were compared with 2 BA children to each case as the control group(total 30 children) on the basis of following criteria : the same age, he/she attended Dae-Dong hospital or local medical center after 8 years old, birth weight was more than 2,500 g. Asthma severity was modified from 2003 Guideline for management of childhood asthma at The Korean Academy of Pediatric Allergy and Respiratory Disease. PFT(Pulmonary function test) were measured using a portable spirometer. The value are expressed as percentage predicted using standard reference values. Total IgE and specific IgE Ab. were assessed by the PRIST method and MAST Allergy Test. Methacholine challenge test and PC20 was assessed by the Masterlab. Results : LBW group had : a significantly more severe symptoms between 8 year-old and 18 year-old than control group.(P=0.015) A significantly more used to ICSs(Inhaled corticosteroids) and add-on therapy than control group.(P=0.009)(P=0.0001) There were no significant differences in the PFT and PC(20) and specific IgE Ab. between LBW group and control group. But, total IgE level is higher in the control group than in the LBW group. Conclusion : These results suggest that LBW is a risk factor for the severity of BA. We suggest that infants with birth weight of less than 2,500 g have a more severe risk of asthma during prealdolescent children and aldolescence than who were birth weight of more than 2,500 g. [Pediatr Allergy Respir Dis(Korea) 2006;16:38-46]

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

한우거세우의 도체 육질등급과 쇠고기 이화학적 성질과의 상관관계

김대곤,성삼경,정근기 대구산업정보대학 2000 논문집 Vol.14 No.2

The study was conducted to investigate the effect of castration on the carcass grade of Hanwoo reared under the same feed and feeding condition. The carcass grading scores were measured by the Korean carcass grading standards. The results were compared with respect to Hanwoo by sex. Hanwoo steers showed significantly higher marbling degree than Hanwoo bulls. In Hanwoo steers, moisture content was lower while crude fat was higher than in Hanwoo bulls. Water holding capacity and L value of rib loin were higher in steers than in bulls. Collagen content was higher in bulls than in steers. The degree of hardness and chewiness in steers were significantly lower than those in bulls. Moisture contents and cooking loss of water were decreased where crude fat contents and water holding capacity were increased as marbling degree increased.

갑상선 유두상 암종과 유두상 증식에 대한 S-100 단백 및 EMA 발현에 대한 연구

강대영,김경희,최정목,송규상,강동욱 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

The diagnosis of papillary carcinoma of the thyroid is readily achieved based on a defined aggregate of histopathologic features. A papillary architecture is an imporant but not pivotal component of the diagnosis. The classic nuclear features are the essential diagnostic element. However, both papillary architecture and nuclear features may be encountered in other conditions and produce problems in histopahologic interpretation. A papillary architecture may be found within a follicular or an oncocytic neoplasm of within the nodules of hyperplastic nodular goiter. Moreover, there may be scattered cells within several thyroid lesions that display some of the nuclear characteristics of papillary carcinoma. The distinction of these lesions from papillary carcinoma is important for differential diagnosis. Thus, the availability of supportive diagnosic evidence would be helpful. Immunoreactivity for S-100 and epithelial membrace antigen was investigated in 16 papillary thyroid carcinomas(PTC), including three examples of the follicular variant, 16 cases of papillary hyperplasis, 8 follicular adenomas, and 6 follicular carcinomas. The degree of reaction was rated on a four point scale: +++, most cells(>51%) have positive staining; ++, 11-50% of cells have positive staining; +, less than 10% of cells have positive staining; -, no positive staining is present. The results are summarized as follows: 1) All the papillary carcinomas showed intense nuclear and cytoplasmic expression of S-100 (+++; 7 cases, ++; 9 cases), and EMA was expressed(++; 7 cases, ++; 6 cases, +; 3 cases). 2) In the cases of papillary hperplasia, papillary foci were generally negative for S-100 and EMA(12/16 ; -). In some areas, they were focal, of weak intensity, and showed preferential apical cytoplasmic location of oncocytic change. 3) Four of eight follicular adenomas expressed S-100(++;2 cases, +; 2 cases), whereas four were completely negative. EMA was also expressed(++; 2 cases, +; 3 cases). 4) Follicular carcinoma showed variable expression of S100 and EMA Five of six follicular carcinomas expressed S-100(++; 2 cases, +; 3 cases), whereas one was negative. EMA was also expressed(++; 2 cases, +; 1 case). In conclusion, in the distinction of papilary carcinoma from papillary hyperplasia, we would recommend an initial histological appraisal, with immunohistochemical staining of S100 and EMA in difficult cases.

조혈모세포이식환자에서 조혈기능의 회복과 관련된 인자들에 관한 연구

정기주,강명수,권기두,김경하,이상철,김현정,배상병,김찬규,이남수,이규택,원종호,홍대식,박희숙 순천향대학교 의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.1

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퉁퉁마디로부터 염에 의하여 유도되는 Aldolase 유전자의 분리 및 발현분석

차준영,네티 엘마와티,김순길,이증주,임채오,정우식,이곤호,손대영 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Soil salinity is one of the most serious abiotic stresses limiting the productivity of agricultural crops. To cope with salt stress, plants respond with physiological, developmental and biochemical changes, including the synthesis of a number of proteins and the induction of gene expression. Salicornia herbacea is a halophytic plant that grows in salt marches and on muddy seashores. In order to understand the biochemical and molecular mechanisms of salt tolerance in S. herbacea, we isolated several genes that involved in the salt tolerance by mRNA differential display.Here we report the cloning of a cDNA encoding fructose-1, 6-bisphosphate aldolase, named ShADL, which is 1293 bp long and contains an open reading frame consisted of 359 amino acids with calculated molecular mass of 39 kDa. ShADL protein showed 86% identity with Arabidopsis and 78% with aldolase of common ice plant. Northern blot analysis revealed that the transcript of ShADL gene was increased dramatically depending on the NaCI concentrations.

個人所藏 銀製葡萄獸禽文小甁에 관한 硏究

서정호,송현정,김대환 공주대학교 문화재보존과학연구소 2004 문화재과학기술 Vol.3 No.1

銀製葡萄獸禽文小甁 新例는 통일신라의 대표적인 문양으로 구성된 것이며, 그 제작기법이 우수하고 예술성도 뛰어나 금속세공기술 연구에 있어서 중요한 자료이다. 또한 출토사례가 많지 않아 매우 희귀한 예라고 할 수 있다. 이 병은 개인 소장품으로 출토당시부터 어깨부 한쪽이 부식되어 박락되어 있었다. 장기 보존을 위해서나 전시를 위해서는 추가 부식을 방지하기 위한 保存方案이 필요하다. 유물의 내부구조와 상태를 파악한 후에 유물이 최대한 원형을 유지할 수 있도록 적절한 처리가 이루어져야 할 것이다. The new case of Silver Bottle is composed of representative design of Unified Silla, and because the production technique is superior and the artistic as well, it is the important material for the study of metalwork technique. And it is the rare case, for there aren‘t many excavated cases. This bottle is personal possession and one side of shoulder is corroded and exfoliated at the time of excavation. For the long-term preservation or display, conservation method which prevents additional corrosion is needed. After catching the inner structure and state, proper treatment should be done in order that remains can retain its original form.

TiN 박막 형성에 미치는 ion beam 조사 효과

안병건,추관식,문두수,안정식,김영대,김형자,이규용 釜慶大學校 2002 釜慶大學校 論文集 Vol.7 No.-

Low energy high current N_2 ion beam was used to modify the morphology and chemical composition of cemented carbide WC-Co(Co:10 wt%, TiC + TaC:15 wt%, WC:bal) hard material surface for the fabrication of the TiN thin film by sol-gel method. The effects of ion beam treatment on preparation of the TiN thin film by sol-gel mothod were investigated by XPS, SEM, AFM and GXRD. According to the N_2 ion beam bombardment, the surface roughness was increased and TiC binders in WC were dissolved. The dissolved Ti was recombind with nitrogen ion to form the TiN nucleuses which were acted as seeds for the forming of the TiN thin film on the cemented WC-Co surface. Besides, the sputter deposited Ti interlayer prevented the diffusion of TiO_2 sol into the porous WC-Co during the formation of TiN thin film.