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林采旿,鄭圭和,宋相鎬 여수대학교 1991 論文集 Vol.5 No.-
Microinjection of foreign DNA into soybean protoplasts was attempted. Among ten enzyme mixtures evaluated for the isolation of protoplasts from immature cotyledon of soybean, the mixture of 0.1% pectolyase Y23, 0.2% cellulase R10, and 1% hemicellulase gave the highest protoplast yield. DNA from a bacterial plasmid can be successfully transferred into the protoplasts by capillary microinjection. However, injected cells were not divided in culture.
Screening for Highly Susceptible Soybean Genotypes to Agrobacterium tumefaciens
LEE, SUNG HO,LIM, CHAE HO,CHUNG, GYU HWA,WON, JONG LAC,PARK, JOONG SUK,KIM, ZHOO HYEON 경상대학교 유전공학연구소 1989 遺傳工學硏究所報 Vol.8 No.-
This experiment was conducted to screen the highly susceptible soybean genotypes to Agrobacterium tumefaciens. Plants from 703 lines and 20 cultivars were inoculated with Agrobacteriun tumefaciens Nopaline type strain c58 and Octopine type strain 15955. About 97% of tested lines were infected by Agrobacteriun tumefaciens strain c58, which only two lines, B678 and B679 responded to Agrobacteriun tumefaciens strain 15955. The number of highly susceptible lines with higher than 50% tumorigenesis and greater than 1 mm tumor size was 53, while 20 lines were not infected at all. Among the highly susceptible group, the lines 53, 79, 463, 551, 674, 675, 677, 678, and 679 could be used for transformation studies due to their high tuomrigenesis and large tumor size. The lines selected in the field test according to the degree of susceptibility showed the same responses to Agrobacterium infection in vitro.
전통발효식품으로부터 Chitin 분해 미생물의 분리 및 특성 규명
고보경,최인순,이상현,임채오,이성호,갈상완,최영주 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-
A bacterial strain CJ-3 which produced chitinase was isolated from Korean traditional soy sauce. Using 16S rDNA analysis, the strain CJ-3 was identified as Bacillus atrophaeus. The approximate molecular weight of the putative chitinase enzyme was 31.0 kDa and the enzyme activity was remarkably induced by addition of colloidal chitin (0.5, 1.0, 2.0%). The antioxidant activity was increased 53% by the browning reaction products of B. atrophaeus CJ-3. Escherichia. coli lipopolysaccharides (LPS)-induced production of nitric oxide (NO) was reduced up to 45% by the browning reaction product in RAW264.7 macrophage. Inhibition of cell viability in the presence of LPS was recovered to normal level by the browning reaction product. These results suggest that browning reaction of B. atrophaeus CJ-3 plays an important role for activation of immune system. B. atrophaeus CJ-3 exhibited optimum temperature and pH of 37℃ and pH 7.8~8.0, respectively. The major intracelluar free amino acid was determined to be glutamate.
Jung Eun Hwang,임채오,Chan Ju Lim,Huan Chen,제지현,송치은 한국분자세포생물학회 2012 Molecules and cells Vol.33 No.2
Dehydration-responsive element-binding proteins (DREBs) regulate plant responses to environmental stresses. In the current study, transcription of DREB2C, a class 2 Arabidopsis DREB, was induced by a superoxide anion propagator, methyl viologen (MV). The oxidative stress tolerance of DREB2C-overexpressing transgenic plants was significantly greater than that of wild-type plants, as measured by ion leakage and chlorophyll fluo-rescence under light conditions. The transcriptional activity of several ascorbate peroxidase (APX) genes as well as APX protein activity was induced in DREB2C overexpressors. Additionally, the level of H2O2 in the overexpressors was lower than in wt plants under similar oxidative stress conditions. An electrophoretic mobility shift assay and transient activator-reporter assay showed that APX2 expression was regulated by heat shock factor A3 (HsfA3) and that HsfA3 is regulated at the transcriptional level by DREB2C. These results suggest that DREB2C plays an important role in promoting oxidative stress tolerance in Arabidopsis.
김선호,이경희,김경은,정미순,임채오,이신우,정우식,Kim, Sun-Ho,Lee, Kyung-Hee,Kim, Kyung-Eun,Jung, Mi-Soon,Lim, Chae-Oh,Lee, Shin-Woo,Chung, Woo-Sik Korean Society of Life Science 2007 생명과학회지 Vol.17 No.9
칼모둘린은 칼슘과 결합하는 센서로써 다양한 칼모둘린 결합 단백질들과의 상호 작용을 통하여 세포 내에서 여러가지 기능을 조절한다. 진핵 생물들은 많은 종류의 칼모둘린 결합 단백질을 가지고 있기 때문에 이러한 단백질들의 분리와 특성 규명이 중요하다. 이미 여러 가지 방법들을 이용하여 칼모둘린 결합 단백질들이 분리되었고 이미 알려진 단백질의 구조적인 유사성을 토대로 더 많은 단백질들이 예측되었다. 우리는 애기장대에서 칼모둘린 결합 단백질의 분리와 특성 규명을 위해 형광 단백질과 융합된 칼모둘린 과발현 형질 전환체를 제조하여 공촛점 현미경과 Western blot 을 이용하여 과발현 형질 전환체를 선별하였다. 또한 형질 전환체 내의 칼모둘린이 칼모둘린 결합 단백질과 상호 작용함을 pull-down 분석을 통해서 확인하였다. 이러한 결과들을 토대로 칼모둘린 과발현 형질 전환체를 이용하여, 칼모둘린과 상호 작용하는 여러 가지 칼모둘린 결합 단백질들을 분리할 수 있을 것으로 기대된다. Calmodulin (CaM), a ubiquitous calcium-binding protein, regulates diverse cellular functions by modulating the activity of a variety CaM-binding proteins (CaMBPs). Because eukaryotes have multiple CaMBPs, it is important to isolate and characterize them in different tissues and conditions. So far a number of CaMBPs have been identified through classical screening methods. Many classes of proteins have been predicted to bind CaMs based on their structural homology with already known targets. In an effort to develop a method for large-scale analysis of CaMBPs in Arabidopsis, we have generated a transgenic plants overexpressing AtCaM2-GFP. We performed protein pull-down assay to test whether exogenously expressed AtCaM2-GFP proteins can interact with CaMBPs. The exogenously expressed AtCaM2-GFP could strongly interact with a CaMBP, AS1 protein. This result suggests that AtCaM2-GFP in transgenic plants may interact with many CaMBPs in plant cell. Therefore, we will be able to isolate kinds of CaMBPs by using these transgenic plants in many different tissue and environments.