Lactobacillus pentosus 발효에 의한 담쟁이덩굴 줄기 추출물의 항산화 및 세포보호 효과

박소현 ( So Hyun Park ),성준섭 ( Joon Seob Seong ),이건수 ( Keon Soo Lee ),박영민 ( Young Min Park ),현송화 ( Song Hua Xuan ),차미연 ( Mi Yeon Cha ),강희철 ( Hee Cheol Kang ),박수남 ( Soo Nam Park ) 대한화장품학회 2017 대한화장품학회지 Vol.43 No.3

본 연구에서는 담쟁이덩굴 줄기 70% 에탄올 추출물과 발효균주 Lactobacillus pentosus를 이용하여 발효시킨 담쟁이덩굴 줄기 발효추출물에 대하여 항산화 및 세포보호 효과를 측정하였다. 1,1-Diphenyl-2-picrylhydrazyl (DPPH)를 이용한 자유라디칼 소거 활성(FSC₅₀)은 담쟁이덩굴 줄기 추출물 및 발효추출물이 각각 42.3 및 34.5 μ g/mL로 발효 후의 라디칼 소거활성이 약 18.4% 더 높게 나타났다. Lumiol-의존성 화학발광법을 이용한 Fe³⁺-EDTA/H₂O₂계에서의 총 항산화능(OSC₅₀) 평가에서도 담쟁이덩굴 줄기 추출물과 발효추출물은 각각 2.6 및 2.5 μ g/mL로 발효 후가 약 4.2% 정도 더 높은 총 항산화능을 나타냈다. ¹O₂로 유도된 적혈구 세포손상에 있어서 추출물 및 발효추출물의 세포 보호 효과(τ₅₀)는 50 μ g/mL에서 각각 126.4 및 173.0 min을 나타내어 발효 후 세포 보호 효과가 약 34.0% 더 높게 나타났다. 발효추출물은 지용성 항산화제로 알려진 (+)-α-tocopherol (43.4 min)보다도 3.9배 높은 세포 보호 활성을 보여주었다. 사람 섬유아세포인 Hs68을 대상으로 elastase 저해 활성을 조사하였다. Elastase 저해 활성(IC₅₀)은 담쟁이덩굴 줄기 추출물과 발효추출물에서 각각 873.6 및 687.8 μ g/mL로 발효 후에 elastase 저해 활성이 약 21.3% 더 높은 것으로 나타났다. 이상의 결과들은 담쟁이덩굴 줄기 발효추출물이 항산화 작용과 더불어 주름개선 효과를 가지는 천연 화장품 소재로써 응용 가능성이 있음을 시사한다. In this study, the antioxidant activities, cellular protective effects, and inhibitory effects on elastase of non-fermented and fermented extracts of Parthenocissus tricuspidata (P. tricuspidata) stem using Lactobacillus pentosus were investigated. The free radical scavenging activities (FSC₅₀) of non-fermented and fermented extracts were 42.3 and 34.5 μg/mL, respectively, in which the activity after fermentation was approximately 18.4% higher. Reactive oxygen species (ROS) scavenging activities (OSC₅₀) in Fe³⁺-EDTA/H₂O₂ system of non-fermented and fermented extracts were 2.6 and 2.5 μg/mL, respectively. The activity after fermentation was approximately 4.2% higher. In the ¹O₂-induced cellular damage of erythrocytes, the cellular protective effects (τ₅₀) of non-fermented and fermented extracts were 126.4 and 173.0 min at 50 μg/mL, respectively. The activity after fermentation was approximately 34.0% higher. The effect of fermented extract was 3.9 times higher than (+)-α-tocopherol (τ₅₀ = 43.4 min), known as a lipophilic antioxidant at 50 μg/mL. The inhibitory effect of elastase was investigated to predict the anti-wrinkle efficacy using Hs68 human fibroblasts cells. The elastase inhibitory activities (IC₅₀) of non-fermented and fermented extracts were 873.6 and 687.8 μg/mL, respectively, and the activity after fermentation was approximately 21.3% higher. These results indicated that fermented extract of P. tricuspidata stem has potentials as natural cosmetic ingredients with antioxidant and anti-wrinkle effect.

Subcritical water extraction (SWE) of anthocyanin from blueberries (Vaccinium corymbosum)

Hye-Ji Kang,Seon-Woo Kim,Ha-Un Yoo,Na-Yeon Kim,So-Yoon Yee,Min-Jung Ko,Myong-Soo Chung 한국산업식품공학회 2017 학술대회 및 심포지엄 Vol.2017 No.11

Subcritical water extraction (SWE) is an eco-friendly new extraction technology because it does not contain harmful organic solvent and has high extraction efficiency in a short time compared with conventional extraction methods. Blueberries (Vaccinium corymbosum) are widely known as superfood due to rich source of anthocyanin (malvidin-3-o-galgctoside) and antioxidant activity. In this study, optimal extraction condition of SWE from blueberries was determined and compared with the conventional extraction methods. SWE was carried out using a Dionex Accelerated Solvent Extractor (ASE, Model 350) under conditions of temperatures (110, 130, 150 and 170°C) and times (1, 3, 5 and 10 min). Total anthocyanin of SWE extracts was compared with hot water (60°C, 1 h) extract and pressed juice extract. The total anthocyanin content was determined by pH differential method. Considering both the extraction time and temperature conditions, the highest content of total anthocyanin content was 0.455 mg/g FW Vaccinium corymbosum at 130°C for 3 min. At high temperature and long extraction time, the anthocyanin in the blueberries will undergo thermal degradation due to low stability of anthocyanin at extreme condition. Besides, maximum yield of anthocyanin from blueberries using SWE was about 1.2 and 3.8 times more higher than hot water extract and pressed juice extract, respectively. Therefore, SWE is faster and more efficient method to extract anthocyanin from blueberries than conventional extraction methods. This study shows a possibility of SWE applied to food processing industry.

미학과 사회적 이슈를 연계한 전시디스플레이 작품 연구 -저출산에 대한 인식 개선 프로젝트 "나쁜 엄마, 최고의 아빠"의 전시디자인 프로세스를 중심으로-

강미선,박수빈,윤정원,오정은,이소원,이진민,신은별 숙명여자대학교 디자인연구소 2017 숙명디자인학 연구 Vol.23 No.-

본 연구는 전시디스플레이 디자인 분야에 미학을 기반으로 예술적, 사회적, 디자인적 측면으로 다각적으로 접근하여 전시 디자인 분야의 색다른 코딩 프로세스를 체계화하고, 동시에 기존의 전시와는 차별화되는 새로운 전시 공간 프로세스를 제안하는데 목적이 있다. 본 연구의 대상은 2016년 9월-12월까지 15주간 숙명여자대학교 미술대학 환경디자인학과<전시디스플레이 디자인>교과목에서 진행된 프로젝트 중 일부이다. 본 연구의 과정은 총 3과정으로 이루어져 있으며, 미학적 개념과 사회적 문제, 전시관련 학문에 대한 이론적 고찰을 한 뒤 이를 전시디자인에 코딩하는 작업을 선행하였다. 두 번째로는 코딩한 작업을 바탕으로 전략적 모티브를 빛으로 선정하고, 전시디자인 전략게획을 진행하였다. 마지막으로 자료를 바탕으로 만든 스토리텔링을 기반으로 전시전개구성을 하고 이를 통해 스토리가 있는 차별화된 공간구성을 하였다. 로고디자인, 포스터디자인, 리플렛디자인, 영상 디자인 등 전시디자인 E.I작업을 통해 전시를 표현하였다. 끝으로 본 연구는 '미학 + 사회문제 + 전시디자인의 코딩'이라는새로운 창의적 전시 디자인 프로세스를 제안하였고, 이는 미학과 사회문제의 이론적 고찰을 반영한 전시디스플레이 프로세스 개발에 있어 향후 기초 자료로 활용될 것으로 기대한다.

회전원통수조 실험에서 회전속도에 따른 표류각속도

소선섭,강민구,김영은,조미순 公州大學校 基礎科學硏究所 2003 自然科學硏究 Vol.10 No.-

본 논문은 Kazuo Ukaji와 Katsumi Tamaki의 논문 중 회전속도(回轉速度)와 표류각속도(漂流角速度)의 관계를 기초로 하였다. 그들에 따르면, 회전삼중수조 실험값이 수치모델값보다 열손실에 의해 약 10% 정도 작고, Ω(회전속도)값이 증가하면 ω(표류각속도)는 점차적으로 감소함을 알 수 있었다. 본 논문에서는 그것을 실험으로써 증명하고자 하였다. 온도차(∆T)를 일정하게 유지하고 회전속도를 증가시키면서 파를 관찰하였다. 그 결과, 회전속도(回轉速度)를 크게 할수록 파의 표류각속도는 점차 감소하는 경향을 나타냈다. 또 회전속도에 따른 표류각속도의 기울기는 온도차가 작은 경우에 기울기가 더 높게 나타났다. This essay was based on relation of the drift angular speed and angular velocity in the essay of Kazuo Ukaji and Katsumi Tamaki. According to them, because of heat loss, rotating fluid annulus experimental value is lower about 10 percent than numerical model value, we could know that ω is dwindling if Ω increases. In this essay, we observed to certify it as an experiment. We kept differences of temperature changelessly, and observed form of waves as increasing turning number of times. By the result, when we changed greatly volutions per minute, observed hat the drift angular speed of wave is dwindling. Also, by the result, we observed tilt of a drift angular speed by angular velocity, slant in case difference of temperature is low.

남자대학생의 흡연태도와 행동에 관한 연구

강경진,김정미,민혜영,안소진,오경숙,은경희,이현,이희선,홍주미,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

There have been so many researches about problems of cigarettes and relation of smoking and heath since 1950's and generally known that smoking is one of the worst factor of increasing disease and mortality. No matter what such many proofs about various problems of smoking have been researched, smoking rate of current male collage students has not been decreased. Besides, most of the researches about cigarette use and its addiction have been focused on the adolescent or adult women smokers or adult male, not male collage student. Therefore, the subject of this study is about smoking attitude and smoking behavior of male collage students by general features. Smoking attitude was analyzed by modifying 'Guidelines for the content of tobacco smoking surveys of the general population'(WHO, 1982). Two hundred of male collage students participated to the distributed questionnaire for one week in May. The smoking attitude was categorized into duration of smoking and number of cigarette per day(CPD), and nicotine dependency, investigated by Fagerstrom Tolerance Questionnaire(FTQ) (Fagerstorm & Schneider, 1989). All statistics were performed by procedure of SAS according to number, percentage, mean, standard deviation, and ANOVA. The results are as follows: 1. Smoking attitude level was mean 25.73±5.15 marks, ranging from 13 to 39 marks of the total 40 marks. 2. Duration of cigarettes, number of cigarette per day, and nicotine dependency were mean 5.14±2.84 years, 12.88±6.85 pieces, and 4.78±1.69, respectively. 3. Smoking attitude showed significance according to general features such as marital state and allowance, but the significance according to marital state is not valuable because the numerical difference is so big between married one and single that they cannot be compared with each other. 4. Duration of smoking was significant according to general features such as age and allowance. 5. Smoking amount showed significance the factors of age only. 6. ll1ere was no significance in nicotine dependency according to general features. As a result, smoking attitude of male collage student in this study shows moderate level. Smoking attitude and duration is significant according to allowance, and smoking amount is significant according to age. Nicotine dependency according to general features is not significant but comparing with the preceding study about adult male, male collage students in this study are respectively more nicotine-dependent than adult male smokers. In conclusion, the right allowance usage strategies and effective heath education focused on the young are needed. In addition, helpful programs and information spreading strategies are necessary for establishing higher level of smoking attitude. At last, systemic regulations in daily living for ""anti-nico-dependent"" and perspective reforming of male university student are needed for reducing nicotine dependency.

제2형 콜라겐으로 경구관용을 유도한 관절염 모델 마우스의 비장림프구내의 보조자극인자 및 STAT/SOCS 신호전달 인자의 발현 양상조사

이강은,황수연,민소연,김호연 Official Journal of the Korean society for immunol 2003 Immune Network Vol.3 No.3

상부요로계감염 환자에서 Gallium-67 Citrate 스캔 및 Tc-99m Dimercaptosuccinic Acid(DMSA)스캔의 임상적 의의

이강욱,강민규,장윤경,양종오,이상주,박기현,소영,신영태 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2

It is often not certain whether the focal defect of renal cortex of Tc-99m Dimercaptosuccinic acid(DMSA) renal scan in patients with acute pyelonephritis means the cortical scar by previous inflammation or recent active inflammatory lesion. Gallium-67 citrate(Ga-67) also has been known to be accumulated at the active inflammatory lesion. In order to evaluate the usefulness of the Ga67 and Tc-99m DMSA renal scan in patients with acute pyelonephritis, we reviewed the results of both study and clinical findings. Nintecn patients with typical clinical findings of acute pyelonephritis and admitted to our institute from Jan. 1998 to Nov. 1998 were included in this study. Tc-99m DMSA and Ga-67 scan were performed mean 3.3 and 7.3 days after admission. Eleven of 19 patients (57.9%) showed abnormally increased focal renal uptake on the Gallium-67 citrate scan and 14 of 19 patients(73.7%) had a cortical defect on the kidneys in Tc-99m DMSA scan. Seven(36.8%) patients had an abnormality in both study. Seven(36.8%) patients had an abnormality only in Tc-99m DMSA scan and 4 (21.1%) patients had an abnormality only in Ga-67 scan. Of 7 patients who had an abnormality on both scan, 5 patients had. an abnormality at same region in both scan and 2 patients had an abnormality at the different sites on the kidneys. With the above results, we speculate that Ga-67 scan and Tc-99m DMSA scan play a complementary role in the patients with upper urinary tract infection.

한국에서 메스암페타민 남용 또는 의존 환자의 인격장애에 관한 연구

김소영,권성민,조성남,권도훈,강병조,김형태 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.2

연구 목적 : 메스암페타민 남용 또는 의존이 없는 군과 있는 군 사이의 인격장애의 유병율을 알아보고, 메스암페타민 남용 또는 의존군에서 동반 인격장애가 그들의 전반적 기능 및 일반적 특성에 어떠한 영향을 미치는지 알아보고자 하였다. 방 법 : 대상은 정상대조군에서 메스암페타민 남용 또는 의존이 없는 50명과 정신병원에 입원한 메스암페타민 남용 또는 의존환자 50명이었다. 메스암페타민 남용과 전반적 기능에 대한 평가는 DSM-Ⅳ에 의거하였고, 인격장애를 평가하기 위해 대상군 모두에서 PDQ-4+를 시행하였다. 결 과 : 정상대조군은 34%에서 적어도 1개 이상의 인격장애를 나타내었으며, 한 사람 당 평균 3.94개였다. 메스암페타민 남용 또는 의존군은 64%에서 적어도 1개 이상의 인격장애를 보였으며, 한 환자 당 평균 4.72개였다. 메스암페타민 남용 또는 의존군에서 동반 인격장애의 유무는 시작 연령, 사용 용량, 그리고 입원 또는 구치 횟수와는 관련성이 없는 것으로 나타났으나, 3개 이상의 동반 인격장애를 가질 경우, 그렇지 않은 군에 비해 전반적 기능이 떨어졌다. 결 론 : 본 연구 결과, 우리나라에서도 일반인에 비해 물질사용장애 환자에서 인격장애를 동반할 가능성이 높으며, 물질사용장애 환자 중에서도 인격장애를 동반한 군이 그렇지 않은 군에 비해 전반적으로 기능이 떨어진다는 결론이 가능하게 되었다. Objectives : The purpose of this study was to examine the prevalence of personality disorders between normal control group and hospitalized methamphetamine abuse or dependent patients and, among the latter, the influence of comorbid personality disorder on their global assesment function and general characteristics. Methods : Subjects were 50 non-methamphetamine abuse or dependent persons in the general population group and 50 methamphetamine abuse or dependent patients admitted to the psychiatric hospital. Methamphetamine abuse or dependence and the global assesment function were diagnosed according to DSM-Ⅳ, and the presence of personality disorder was assessed with the PDQ-4+. Results : In the normal control group, 34% of subjects had at least one personality disorder and the average number of personality disorder was 3.94 per person. In the methamphetamine abuse or dependent group, 64% of the patients had at least one personality disorder and the average number .of personality disorder was 4.72 per patient. The presence of comorbid personality disorder were not associated with onset age, use dosage and the number of admission or arrest among the methamphetamine abuse or dependent group. But, when methamphetamine abuse or dependent patients had more than three personality disorders, the global assessment function was impaired. Conclusion : In our study, substance use disorder patients had the higher possibility of comorbid personality disorder in Korea, and among substance use disorder patients, those with comorbid personality disorders were associated with the greater global impairment.

골격근 근형질세망의 ATP유도 ^45Ca-uptake에 대한 Thapsigargin및 Vanillylnonanamide의 영향

황의강,배소현,홍장희,허강민,김진회,이재흔,석정호 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2

To investigate the effect of thapsigargin(THP) and vanillylnonanamide(VN), derivative of capsaicin, on the ATP-induced ^45Ca-uptake in the sarcoplasmic reticulum(SR) of the skeletal muscle, the SR vesicles were prepared from the back muscle of the rabbit, and ^45Ca-uptake was carried out. The results as follows: ATP-induced ^45Ca-uptake of skeletal muscular SR was significantly increased by 1 mM ATP. It was significantly blocked by 5 μM THP, but slightly decreased by 20 μM VN. The combined effect of THP and VN on the 45Ca-uptake of the SR vesicles was much potentiated than the sum of each effect of them. The above results suggest that the action of VN, being slightly influential to ATP-induced Ca-uptake but potentiating the effect of THP on the Ca-uptake, might be related with direct perturbation of the SR membrane or exposure of the THP-binding site.

교액성 폐쇄공탈장 1예

조태웅,민영돈,김경종,정권율,강성인,류소연,임성철 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.1

Obturator hernia is a rare pelvic hernia, occuring most frequently in elderly, extra-thin or debilitated women. Because of the anatomic character of the obturator foramen and the nonspecific symptoms in the presence of the hernia, the risk of strangulation of the herniated bowel is high, which leads to high morbidity and mortality rate. The authors experienced an unusual case of left obturator hernia with strangulation of the small bowel in an 80-year old female. The hernia was diagnosed by abdomino-pelvic computed tomography (CT) scan. Through the lower midline transperitoneal approach, resecting the strangulated small bowel, the hernia sac was ligated and the obturator foramen was repaired using adjacent broad ligament. We report these findings with a brief review of the literature.