전방 불안정성 견관절에서 이두박근의 근전도 활동성

김승호,하권익,김현숙,김선우,박종혁,김영민 대한견.주관절학회 2000 大韓肩.주關節學會誌 Vol.3 No.2

子宮內膜搔爬術 21例의 鍼麻醉效果에 대한 臨床的 硏究

金在圭,金相佑,李宜暻,金建植,金相佑,南相永 경희대학교 1997 慶熙醫學 Vol.13 No.1

Proteomic Approach to Analyzing Flooding Stress in Soybean (Glycine max(L.) Merr.)

Kim,Tae-Seon,Cho,Seong-Woo,Lee,Jae-Eun,Kim,Yook-Han,Cho,Kun,Choi,Jong-Soon,Chung,Keun-Yook,Song,Beom-Heon,Jong,Seung-Keun,Kim,Hong-Sig,Woo,Sun-Hee 한국작물학회 2005 한국작물학회 학술발표대회 논문집 Vol.2005 No.-

Multi‑tissue lipotoxicity caused by high‑fat diet feeding is attenuated by the supplementation of Korean red ginseng in mice

Seon‑A Jang,Seung Namkoong,이성률,Jin Woo Lee,Yuna Park,Gyeongseop So,Sung Hyeok Kim,Mi‑Ja Kim,Ki‑Hyo Jang,Alberto P. Avolio,Sumudu V. S. Gangoda,Hyun Jung Koo,Myung Kyum Kim,Se Chan Kang,Eun‑Hwa Sohn 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.1

Background Excessive intake of fat, one of the causes of obesity, is associated with low-grade infammation in various susceptible organs and eventually causes tissue toxicity. This study examines the multifaceted suppressive efects of Korean red ginseng extract (KRG) on high-fat diet (HFD)-induced lipotoxicity and infammatory responses in the aorta, liver, and brain. Methods Male C57BL/6 mice were fed HFD with or without KRG for 12 weeks. The improvement efect in KRG on lipotoxicity and infammatory potential was determined in the blood and the aorta, liver, and brain tissues. Results KRG signifcantly inhibited 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity by >20% in vitro. KRG supplementation suppressed HFD-associated body weight gain, lipid profle changes, and excessive fat deposition in the liver and increased leptin, insulin, and ALT levels in the blood. Infammatory markers in the aorta, liver, and brain were also signifcantly reduced by KRG treatment. In microvascular endothelial cells, the 15% cyclic stretch-mediated upregulation of ICAM-1 and vascular cell adhesion protein-1 (VCAM-1) expression was signifcantly attenuated in the presence of KRG. Conclusion KRG supplementation attenuates HFD-mediated body weight gain, lipid profle changes, and multi-tissue infammatory responses.

핵전달 방법에 의한 Aspergillus 속 종간잡종 형성에 관하여

김선우,이숙영,양영기 조선대학교 기초과학연구소 1990 自然科學硏究 Vol.13 No.1

핵전달 방법에 의해 A. nidulans FGSC 514와 A. niger의 종간잡종을 얻었고 그들 특성을 관찰한 결과는 다음과 같다. 1. A. nidulans FGSC 514의 원형질체 형성은 삼투안정제인 0.6M KCI과 0.5% Novozym 234를 2시간 처리하여 2x10^7 원형질체를 얻었다. 2. A. niger의 원형질체 형성은 삼투안정제로 0.6M KCI, 0.5% Novozym 234, 0.3% β-glucuronidase와 0.4% BSA를 2시간 처리하여 6x10^7 원형질체를 얻었다. 3. A. niger의핵을 추출하여 A. nidulans FGSC 514 원형질체를 형질전환시켜 3.5%의 형질전환률을 얻었다. 4. 형질전환체의 섬유소 분해효소 활성은 CMCase, avicelase와 pectinase에서 대체적으로 증가하였고 β-glucosidase에서는 모균주보다 약간 감소하는 경향을 보였다. 5. 형질전환체의 건조균체량은 모균주에 비해 대체적으로 높게 나타났고 배양 10시간부터 25시간까지 급격한 증가를 보였으나 40시간 후에는 거의 증가하지 않았다. 6. 형질전환체의 DNA 함량은 1.5∼2.23㎍/10^7pts로 모균주에 비해 약 1.4∼2.0배 증가하였다. 7. 세포외 수용성 단백질은 전기영동 양상은 형질전환체들은 유사하나 모균주의 A. nidulans FGSC 514와 A. niger과는 양상이 다른 중간형태를 나타내었다. The properties of interspecific hybrids obtained by nuclear transfer method between A. nidulans FGSC 514 and A. niger wild type were analyzed by enzyme activity, protein determination, dry weight, DNA content, and total solube protein. The protoplast formation from A. niger was 6x10^7 pts with the treatment of 0.6M KCI, osmotic stabilizer, 0.5% novozym 234, 0.3% β-glucuronidase and 0.4% BSA for 2 hrs. When the isolated nuclei of A. niger wild type were transformed the protoplasts of A. nidulans FGSC 514, transformation frequency was 3.5%. It was found that the enzyme activities in degradation of cellulose and polysaccharide were increased in CMCase, avicelas and pectinase but decreased in β-glucosidase. The strains in which enzyme acttivity in general had a tendency of increasing apecific activity and protein content. The dry weight of transformants were increased more than the parent and increased fast from 10 to 25 hrs. The DNA content of transformants were 1.5∼2.23㎍ and 1.42∼2.04 times more than that of both A. nidulans FGSC 514 and A. niger wild type. Electrophoretic patterns of extracellular solube protein were different between parental and transformant strains.

1920년대 초 '開闢' 誌에 등장하는 주택개량론의 성격

김명선,심우갑 대한건축학회 2002 대한건축학회논문집 Vol.18 No.10

This study investigates the discourses of "Housing Improvement" in 「KaeByeok」 from 1920, June to 1926, August. They are produced by "Culture Movement" advocates, whose main concern was in improvement of housing as a social residence from traditional to modern style, rather than spacial improvement of housing itself. So the discourses dealt with housing as a installation or a supplement for modernization of family life, and insisted on improving traditional housing to support modern life style. These situation are understood as unique character of housing improvement discourses of early 1920s as well as distinguished property from those produced by architects after mid 1920s.

사무공간의 통합유니트 구축을 위한 조명유니트 도출에 관한 연구

김선숙,신선준,김지현,양인호,김광우 대한건축학회 2005 대한건축학회논문집 Vol.21 No.1

The purpose of this study is to develop the lighting units for workspace and to propose their application method at the architectural design stage The lighting unit is one of the components of a workspace integrated unit, which can be defined as the planning unit satisfying the environmental comfort criteria of workspace According to the survey of current domestic and foreign office buildings, the luminaire layout types could be classified into the line, spot and square type Various simulation cases were set up considering these layout types, architectural modules, and luminaire types Finally, forty-four lighting units were developed by RADIANCE simulation With these lighting units, the designer would be able to easily predict how his decision about architectural modules influences the lighting design, and can consider other alternatives

응력경로에 따른 섬진강 모래의 탄성특성

김문규,남선우,정현철 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.2

Usually, Many Engineers have used predicting the settlement and displacement using linear elastic modulus(E_(50) calculated by the conventional triaxial test results. But it is results are very diffemt from those of field, which follows deffent stress path and nonlinear behaviour. Therefore, to investigate the dependence of stress path with the variation of confining pressure and relative density on Sum-Jin River sand, local strain triaxial tests are preformed. It is showed as follows ; For the same stress path condition, the stiffness increased with increasing confining pressure and relative density, especially it is more affected by confining pressure. From the relations between Young's modulus and axial strain showed all nonlinear-elastic behaviour and independent on testing conditions, such as, confining pressure, relative density and stress path, etc. The mount of stiffness at very small strain range showed very high value and linear elastic behaviour. And compression results are rather overestimated than that of extention.

상피성장인자가 치주인대세포 및 치은섬유아세포의 증식능에 미치는 영향

김선우 慶北大學校 齒科大學 1996 慶北齒大論文集 Vol.13 No.-

성장인자 가운데 미분화중배엽세포의 활성을 조절하는 것으로 알려진 상피성장인자를 초기배양한 치주인대세포와 치은섬유아세포에 각기 다른 농도와 시간에 따라 주입하였을 때 두 세포의 세포증식능에 미치는 영향을 알아보고 각 조건에 따른 두 세포간의 증식능을 상호 비교해 보고자 본 실험을 실시하였다. 교정치료를 목적으로 내원한 환자의 제 1 소구치 부위의 정상치은을 절제하고, 건강한 제 1 소구치를 발거하여 치은섬유아세포와 치주인대세포를 분리, 배양하여 상피성장인자를 주입시키지 않은 군을 대조군으로 하고 상피성장인자를 각각 1, 10, 50, 100, 200ng/ml로 실험군으로 하여 24시간, 48시간, 72시간 동안 배양하였으며, 각 시간별 배양 6시간전에 10μl/200 ml 5-Bromo-2'-deoxy-uridine을 첨가하여 5-Bromo-2'-deoxy- uridine이 DNA내로 편재되는 양으로써 두세포군의 증식능을 측정하여 다음과 같은 결과를 얻었다. 치은섬유아세포에 상피성장인자를 주입후 24,48,72시간 동안 배양시 각시간별로 농도의존형으로 증가하는 경향을 보였으며 48시간까지 세포의 증식능이 증가 하였다가, 72시간 배양시에는 24,48시간 배양시에 비해 증식능이 전반적으로 감소하는 경향을 보였다. 24시간 배양시에느 1,10Πg/ml를 제외한 모든 실험군에서, 48시간 동안 배양시에는 1Πg/ml를 제외한 모든 실험군에서, 72시간 배양시에는 1, 10, 100Πg/ml를 제외한 모든 실험군에서 대조군에 비해 통계적으로 유의한 차이(P<0.01,P<0.05)를 나타내었다. 치주인대세포에 상피성장인자를 주입후 24,48,72시간 동안 배양시 24시간에서는 농도의존적으로 미약한 증가를 보이다가 200Πg/ml에서 감소하는 경향을 보였으며, 48시간에서는 농도의존적으로 증가하다가 72시간에서는 24시간과 유사한 양상을 보였다., 24, 72시간 배양에서는 48시간 배양에 비해 증식능이 감소하는 경향을 보였다. 24,48,72시간 배양시 모두에서 1Πg/ml를 제외한 모든 실험군에서 대조군에 비해 통계적으로 유의한 차이(P<0.01,P<0.05)를 나타내었다. 치주인대세포와 치은섬유아세포의 증식능을 비교해 보면, 24시간 배양시에 모든 실험군에서 치주인대세포가 치은섬유아세포보다 조금 더 낮은 경향을 나타애었고, 두 세포 상호간에도 모든 실험군에서 통계적으로 유의한 차이 (P<0.01)를 나타내었다. 48시간 배양시에는 모든 실험군에서 치주인대세포가 치은섬유아세포보다 증식능이 더 높은 경향을 나타내었으며, 두 세포 상호간에 통계적으로 유의한 차이 (P<0.01,P<0.05)는 나타내지 않았다. 72시간 배양시에는 1Πg/ml까지 치주인대세포가 더 낮은 증식율을 보이다가 10Πg/ml이상에서는 치주인대세포가 치은섬유아세포보다 그 증식능이 더 높은 경향을 나타내었으며, 두 세포 상호간에 50,200Πg/ml를 제외한 모든 실험군에서 통계적으로 유의한 차이(P<0.01,P<0.05)를 나타내었다. Epidermal growth factor(EGF) is one of polypeptide growth factors. EGF has been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. The purposes of this study is to evaluate the effects of EGF on the human periodontal ligament cells and human gingival fibroblast cells that promote regeneration of periodntal tissue. The mitogenic effects of epidermal growth factor on human periodontal ligament cells and human gingival fibroblasts were evaluated by dentermining the incorporation of 5-Bromo-2'-deoxy-uridine into DNA of the cells in a dose dependent manner. The prepared cells were the primary cultured gingival fibroblast and periodontal ligament cells from humans, the fourth or sixth subpassages were used in the experiments. Cells were seeded in DMEM containing 10% FBS. 1, 10, 50, 100, 200Πg/ml and epidermal growth factor were added to the quiescent cells for 24 hours, 48 hours and 72 hours. They were labeled with 10μl/200μl 5-Bromo-2'-deoxy-uridine for the last 6 hours of each culture. The results of the five determinants were presented as mean and S.D.. The results were as follows: The DNA synthetic activity of human gingival fibroblasts were increased dose dependently by epidermal growth factor at 24 hours, 48hours and 72 hours. The mitogenic effects were similar at the 24 and 48 hours of epidermal growth factor, but the DNA synthetic afctivity of human gingival fibroblasts generally decreased at 72 hours. The DNA synthetic activity of human periodontal ligament cells were increased dose dependently by epidermal growth factor at 24 hours but the DNA synthetic activity decreased at 200Πg/ml of each hour. Generally the maximum mitogenic effects were observed at the 48 hours application of epidermal growth factor. The DNA synthetic activtity of human periodontal ligament cells generally decreased lower at 24,72 hours than at 48 hours the application of epidermal growth factor. In the comparison of DNA synthetic activity between human gingival fibroblasts and human periodontal ligament cells, human periodontal ligament cells had slightly higher proliferation activity than human gingival fibroblasts for a longer time at the high dosage of the epidermal growth factor. In conclusion, epidermal growth factor have important roles in the stimulation of DNA synthesis in human periodontal ligament cells and human gingival fibroblasts, and thus may be useful for clinical applications in periodontal regenerative procedures.

GC/NPD를 이용한 다성분 잔류농약의 동시분석

김우성,이선화,김상엽,정동윤,김재이,이영자,이홍재,정성욱,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.10

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with fiorisil, and finally analyzed simultaneously the organophosphorus pesticides using GC/NPD, Ultra-2 and Ultra-1 fused silica capillary columns were used to separate and identify the products. Recovery of most analytes from soybean sample, taken from pesticide residues well, was greater than(80%) for all except(6) analytes. This method can simultaneously determine multiple pesticides with a high degree of accuracy and precision.