cAMP 농도에 영향을 미치는 수종약물이 파골세포형성에 미치는 영향

소영,고성희,백정화,김관식,정동균 대한구강생물학회 1992 International Journal of Oral Biology Vol.16 No.2

To study the effect of cAMP on the generation of osteoclasts from their precursor cells, the bone marrow cells were isolated from 7 to 9 week-old male mice. The femur and tibia were dissected aseptically and the marrow cavity was flushed with 1 ㎖ of α-minimum essential medium by slow injection. Collected marrow cells were cultured at 1.5-2.0×10^6 cells/well in 24-well plate for 8 days. In experimental group, PGE_2(5×10^-6, 10^-5M), forskolin(10^-5M) or IBMX(10^-4, 10^-5M) were added singly or in combination from the 1st day culture. After cultures, staining for tartrate-resistant acid phosphatase(TRACP)-marker enzyme of osteoclast-was performed according to the modified method of Burstone. The TRACP-positive multinucleated cells(MNC), which have 3 or more nuclei, were counted. The observed results were as follows. 1. In control group. TRACP-positive mononuclear cells were present, but no TRACP-positive multinucleated cells appeared. 2. PGE_2(5×10^-6M) or forskolin(10^-5M) significantly stimulated the formation of TRACP-positive MNC. Moreover, forskolin potentiated the TRACP-positive MNC formation induced by PGE_2 when added simultaneously. 3. IBMX(10^-4, 10^-5M), when added alone, significantly stimulated the formation of TRACP-positive MNC. However, IBMX(10^-5M) added in combination of PGE_2(10^-5M) partially inhibited the TRACP-positive MNC formation induced by PGE_2.

건축설계과정에서 나타나는 협업프로세스에 관한 연구 : 파빌리온 설계사례를 중심으로

김정훈,김소희,임병민,김진아,송정화,오건수 남서울대학교 2007 남서울대학교 논문집 Vol.13 No.4

This study aims to deduce the effective collaborative process of architectural design process based on the specific pavilion design case. The study schematizes the collaborative procedure which is generated at the early stage of design process, analyzes the decision support system and suggests the method for reaching a compromise.Finally, this study suggested collaborative process method of architectural design and provided the basic concept that can be used for the similar projects. Keywords : Collaborative Design, Design Process

여호와의 증인 환자에서 산과적 출혈 후 무수혈 치료 2예

김재령,여소진,이해혁,김정식,김태희,남계현,이권해,이임순,박진화,황경호 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Jehovah's Witness comprise a unique obstetric population. Their refusal of blood stems from an interpretation of a literal translation of the Bible, and it is this belief that puts them at an increased risk of morbidity and mortality if hemorrhage occurs. We report two cases of a Jehovah's Witness who bled massively due to obstetric hemorrhage, refused blood transfusion and had profound anemia. The patients were treated with recombinant human erythropoietin, parenteral iron and oxygen. And they were treated on an intensive care unit with intermittent positive pressure ventilation. We reviewed with literature considering the therapy for acutely anemic patients who refuse transfusion to decrease the duration of the most severe anemia.

녹차 및 결명자 추출물의 교정용 브라켓과 치면 사이의 경계부에서 분리된 mutans streptococci에 대한 항균작용

임성훈,서정순,윤영주,김광원,유소영,김화숙,국중기,이병래,차종희,박재윤 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.5

본 연구는 치아우식증에 관련된 Mutans streptococci 표준균주 및 임상분리 균주의 성장억제를 유도할 수 있는 천연 생약추출물을 검색하기 위하여 실시되었다. 녹차추출물은 CHMC-2032를 사용하였으며, 결명자 추출물은 50% 에탄올을 이용하여 얻었다. 이들 추출물의 Mutans streptococci 표준균주 및 교정환자들에서 브라켓과 치면 사이의 경계부에서 분리된 각각 10 균주씩의 S. mutans 및 S. sobrinus에 대한 최소성장억제농도를 액체배지 희석법으로 구하였다. 그 결과 CHMC-2032의 S. mutans 및 S. sobrinus의 표준 균주, S. sobrinus의 대부분 임상분리 균주에 대한 최소성장억제농도는 5 mg/ml이었다. 그러나 결명자 추출물에 의한 S. mutans 및 S. sobrinus에 대한 세균 성장억제 효과는 미미하였다. 본 연구 결과 치아우식증의 예방적 측면에서 결명자차보다는 녹차를 마시는 것이 유리하며, CHMC-2032를 이용하여 구강양치용액을 제조하여 사용할 경우 교정환자를 포함하여 대부분의 사람에게서 치아우식증 예방 효과가 있으리고 추정된다. Mutans streptococci is the major causative factor in dental caries. Especially, orthodontic patients with fixed appliance are a risk group for dental caries. Because fixed appliances attached on teeth may change the environment of dental plaque, the enamel decalcification or dental caries around the bracket and band is a major side effect of orthodontic treatment. The aim of this study was to search plant extracts that have antimicrobial effect on mutans streptococci. Seed-extract of Casio tora were prepared with ethanol and CHMC-2032, the leaf-extracts from Camellia sinensis extract, was obtained extract, 2 type strains and 20 clinical isolates of mutans streptococci isolated from the interface between orthodontic brackets and tooth surfaces in the orthodontic patients were used in this study. The minimal inhibitory concentration of CHMC-2032 was 5 mg/ml on the S. mutans KCTC 3065, S. sobrinus KCTC 3088, and 8 clinical isolates of S. sobrinus. However, there was no antibacterial effect of seed-extract of C. tora on mutans streptococci. These data suggest that green tea may be more effective than the tea prepared from C. tora in the prevention of enamel decalcification or dental caries around brackets.

항문주위 괴사근막염 수술 후 발생한 두덩결합 골수염의 경험

김지연,김태형,추은주,전민혁,이은정,김종화,박의주,이재철,진소영 대한감염학회 2009 감염과 화학요법 Vol.41 No.3

Osteomyelitis of the pubic symphysis is not common and has often been reported to occur after urological or gynecological procedures, It can be spontaneous in origin but it also is associated with trauma, athletic exertion, pregnancy, and parturition. The early symptoms of osteomyelitis of the pubic symphysis mimic those of osteitis pubis and therefore, the differential diagnosis between these two entities is of clinical importance. A fifty nine-year-old man who had previously received debridement and wide excision of perianal necrotizing fasciitis visited our hospital with pain on both inguinal areas. The core biopsy of the pubic symphysis and aspiration culture were performed, from which Pseudomonas aeruginosa was recovered. He was treated with parenteral ceftazidime for 5 days followed by cefepime for 7 weeks and showed favorable clinical response. To our knowledge this is the first report on osteomyelitis of pubic symphysis resulting from debridement and wide excision of perianal necrotizing fasciitis in Korea.

Development and Evaluation of Non-Hydrous Skin Anal Liquid Crystal using Thermo-Sensitivity Smart Sensor

Kwang-Ho Yoo,Jae-Hwa Hong,So-Hee Eun,Tae-Hwa Jeong,Kwan-Young Jeong 한국유화학회 2014 한국응용과학기술학회지 Vol.31 No.3

In this study, skin permeation enhancement was confirmed by designing it to have a structure and composition similarity to the intercellular lipids that improve miscibility with skin by cross-linked lipids poloxamer. The cross-linked lipids poloxamer was synthesized and analyzed by 1H NMR that structure dose had conjugated pluronic with ceramide3. Active component is released by modification of liquid crystal structure because PPO part, large-scale molecule block of pluronic, has hydrophobic nature at skin temperature of 35℃. Conjugated pluronic with ceramide3 was synthesized using Pluronic F127 and p-NPC (4-nitrophenyl chloroformate) at room temperature yielded 89%. Pluronic(Ceramide 3-conjugated Pluronic) was synthesized by reaction of p-NP-Pluronic with Ceramide3 and DMAP. The yield was 51%. This cross-linked lipids poloxamer was blended and dissolved at isotropic state with skin surface lipids, phospholipid, ceramide, cholesterol and anhydrous additive solvent. Next step was preceded by α-Transition at low temperature for making the structure of Meso-Phase Lamella, and non-hydrous skin analogue liquid crystal using thermo-sensitivity smart sensor, lamellar liquid crystal structure through aging time. For confirmation of conjugation thermo-sensitivity smart sensor and non-hydrous skin analogue liquid crystal, structural observation and stability test were performed using XRD(Xray Diffraction), DSC(Differential Scanning Calorimetry), PM (Polarized Microscope) And C-SEM (Cryo-Scanning Electron Microscope). Thermo-sensitivity observation by Franz cell revealed that synthesized smart sensor shown skin permeation effect over 75% than normal liquid crystal. Furthermore, normal non-hydrous skin analogue liquid crystal that not applied smart sensor shown similar results below 35℃ of skin temperature, but its effects has increased more than 30% above 35℃.

Analysis of Rabies fluorescent antibody virus neutralization test (FAVN) Results from Dogs and Cats: Regarding PetQuarantine regulation change from 1 December 2012

Jeong Hwa So,Ju Yeon You,Young Wook Kim,Jin Kyoung Ha,Deok Ho Kang,Dae Gyun Kim 대한수의학회 2015 대한수의학회 학술대회발표집 Vol.2015 No.-

Prevalence of Enterohemorrhagic Escherichia coli isolated from Bovine Slaughterhouses in Korea

Jeong Hwa So,Jun Man Kim,So Hyun Kim,Sook Shin,Jung Ho Youn,Sang Yup Kim,Kyung Young Choi,Hye Cheong Koo,Yong Ho Park 대한인수공통전염병학회 2008 창립총회 및 학술대회 초록집 Vol.2008 No.1

Expert Consensus on Measures to Promote Physical and Psychological Health among COVID-19-Related Healthcare Workers in Korea using Delphi Technique

Lee So Hee,Noh Jin-Won,Kim Yeonjae,Ryoo Hyun Wook,Park Kyung-Hwa,Park Kyung-Hwa,Lee Shinwon,Cheong Hae Suk,Heo Sang Taek,Hong Ki Jeong,Kim Kyoung-Beom,Kwon Ki Tae 대한감염학회 2022 Infection and Chemotherapy Vol.54 No.2

Background The coronavirus disease 2019 (COVID-19) pandemic has caused health problems and distress among healthcare workers (HCWs), so supportive measures to promote their health and relieve distress are needed. Materials and Methods We conducted two rounds of Delphi surveys with 20 COVID-19-related frontline healthcare professionals and public officials. The surveys evaluated means of supporting HCWs’ health by improving health care systems and working environments in terms of effectiveness and urgency. The validity of the measures was assessed by calculating the content validity ratio. Results The top-priority measures to support HCWs were “secure isolation units capable of treating severe cases” in the facility infrastructure category, “secure nursing staff dedicated for patients in the intensive care units” in the personnel infrastructure category, “improve communication between central office and frontline field” in the cooperation system category, “support personal protective equipment and infection control supplies” in the aid supplies category, and “realization of hazard pay” in the physical/mental health and compensation category. Conclusion There was consensus among the experts on the validity and priorities of policies in the facility, personnel, cooperation, supplies, and compensation categories regarding measures to promote COVID-19 related HCWs’ health. Background The coronavirus disease 2019 (COVID-19) pandemic has caused health problems and distress among healthcare workers (HCWs), so supportive measures to promote their health and relieve distress are needed. Materials and Methods We conducted two rounds of Delphi surveys with 20 COVID-19-related frontline healthcare professionals and public officials. The surveys evaluated means of supporting HCWs’ health by improving health care systems and working environments in terms of effectiveness and urgency. The validity of the measures was assessed by calculating the content validity ratio. Results The top-priority measures to support HCWs were “secure isolation units capable of treating severe cases” in the facility infrastructure category, “secure nursing staff dedicated for patients in the intensive care units” in the personnel infrastructure category, “improve communication between central office and frontline field” in the cooperation system category, “support personal protective equipment and infection control supplies” in the aid supplies category, and “realization of hazard pay” in the physical/mental health and compensation category. Conclusion There was consensus among the experts on the validity and priorities of policies in the facility, personnel, cooperation, supplies, and compensation categories regarding measures to promote COVID-19 related HCWs’ health.

실험연구 : 쥐의 척수에서 Zaprinast의 항통각효과에 대한 아드레날린성 및 콜린성 수용체 역할

윤소정 ( So Jeong Yoon ),김여옥 ( Yeo Ok Kim ),황란희 ( Lan Ji Huang ),최금화 ( Jin Hua Cui ),허봉화 ( Bong Hwa Heo ),정성태 ( Sung Tae Jeong ),윤명하 ( Myung Ha Yoon ) 대한마취과학회 2007 Korean Journal of Anesthesiology Vol.53 No.1

Background: Spinal zaprinast, phospodiesterase inhibitor, has been shown to have an antinociception through an increase of cGMP. The aim of this study was to examine the role of spinal adrenergic and cholinergic receptors on the antinociceptive action of intrathecal zaprinast. Methods: Rats were implanted with lumbar intrathecal catheters. After formalin injection, formalin-induced nociceptive behavior (flinching response) was observed for 60 min. After observing the effect of intrathecal zaprinast, antagonism of intrathecal prazosin, yohimbine, atropine and mecamylamine for the effect of zaprinast were evaluated. Results: Intrathecal zaprinast produced a dose-dependent suppression of formalin-induced flinches in both phases of the formalin test. Intrathecal prazosin reversed the antinociception of zaprinast in phase 2, but not phase 1. Intrathecal yohimbine reversed the antinociception of zaprinast in both phases. Neither atropine nor mecamylamine reversed the antinocicetive action of zaprinast. Conclusions: Intrathecal zaprinast is against the nociceptive state evoked by formalin stimulus. Alpha 2 or alpha 1 adrenergic receptor, but not cholinergic receptors, may be related to the action of zaprinast in the spinal cord. (Korean J Anesthesiol 2007; 53: 85~90)