Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

일렬 자가조혈모세포이식을 받은 다발성 골수종 환자에서 발생한 뇌 톡소포자충증 1례

김계형,송경호,전재현,박완범,박상원,김홍빈,김남중,김인호,오명돈 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Toxoplasmosis is a rare but fatal complication in hematopoietic stem cell transplant recipients, usually associated with allogeneic hematopoietic stem cell transplantation (HSCT). We report a case of cerebral toxoplasmosis in a patient with multiple myeloma, following tandem autologous stem cell transplantation. A 55-year-old Korean male presented with weakness in both legs that had progressed to both arms. A magnetic resonance imaging scan of the brain revealed multiple, variablesized ring-enhancing lesions with surrounding edema in the cerebral hemispheres and brain stem. Stereotactic biopsy revealed bradyzoites of Toxoplasma gondii in the brain tissue. The patient received trimethoprim-sulfamethoxazole, followed by pyrimethamine and sulfadiazine, accompanying treatment for progressive multiple myeloma. Cerebral toxoplasmosis should be considered as one of the differential diagnoses in patients with neurologic signs following autologous HSCT.

Diazepam과 Lidocaine이 노인환자의 기관내삽관시 심전도 및 심맥관계에 미치는 영향

김경희,권성범,신용섭,최세진 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.1

We selected at random 30 geriatric patients who had received operation under the general anesthesia with endotracheal intubation. The patients were divided into 3 groups. Group 1 (control) had received endotracheal intubation without diazepam and lidocaine. Group 2 had received endotracheal intubation with diazepam. Group 3 had received endotracheal intubation with lidocaine. The changes of arterial blood pressure and pulse rate, and the appearance of arrhythmia were compared in each group. The results were as follows : 1. The increase of blood pressure and heart rate were less in group 2, 3 than the control group which showed significant increase of blood pressure and heart rate with endotracheal intubation. 2. The incidence of arrhythmias with endotracheal intubation was less in group 2(20%), group 3(50%) than the control group(80%). 3. The electrocardiographic change in the group 2 and 3 were not significant compared to group 1(control).

학습조직 구축요인과 조직특성의 교육훈련 효과성 연구 : 콜센터 종사자를 중심으로

김경재,정범구,조장현 충남대학교 경영경제연구소 2008 경영경제연구 Vol.31 No.2

With the development of telecommunication technology, and with combination of computer and communication, a new field, which is called a customer care call center , has been created. In the past, the customer center was just passive in the way that their role was just answering to the customers' questions. However, now this has been changed to be active and positive for fulfill customers' satisfaction. Therefore, not only enterprises, but also public institutions and government agencies are having customer care centers as one of methods to maximize customer service. Also, the role of customer care center is extended to marketing activities. The actual proof study on training of call center employees and the transfer of the training to the work has been practiced for this research. The findings of this research are summarized as follows ; first, building factors of learning organization is positively associated with the transfer of training, second, organizational characteristics has moderating effects between building factors of learning organization and training effectiveness. 본 연구는 콜센터 종사자를 대상으로 학습조직 구축요인과 조직특성의 교육훈련 효과성 관계를 알아보기 위하여 학습조직구축요인, 조직특성, 교육훈련 효과성의 개념과 이론을 정리하였고, 연구모형과 가설을 통해 실증분석결과를 제시하였다. 연구결과 학습조직 구축요인이 교육훈련 효과성에 영향을 미치는 것으로 분석되었고, 양 관계에 있어서 조직특성이 부분적으로 조절효과가 있는 것으로 나타났다.

개선된 사람 혈장중 세파클러 농도 정량법을 이용한 세파클러 캡슐의 생체이용률 측정

김태완,송옥경,한선영,Cao, Qing-Ri,박미진,강성화,신관석,Cui, Jing-Hao,이범진 한국약제학회 2005 Journal of Pharmaceutical Investigation Vol.35 No.2

After establishing improved HPLC analytical method ofcefaclor in human plasma samples, a bioavailability study of cefaclor capsules was conducted according to the guidelines of Korea Food and Drug Administration (KFDA). The standard calibration curve using an HPLC with UV detector was constructed in a range of 0.0324--16 μg/m1. The 6% perchloric acid instead of 6% trichloroacetic acid was used to precipitate plasma protein. The HPLC chromatograms were precisely and accurately resolved when spiked with human plasma spiked with cefaclor and cephalexin (internal standard). Twenty healthy male Korean volunteers received two commercial cefaclor capsules, Neocef ' capsule (Jinyang Pharm. Co., Ltd) or Ceclor" capsule {Lilly Korea. Co., Ltd.) at the 250 mg cefaclor in a 2 x 2 crossover study. There was a one-week washout period between the doses. Plasma concentrations of cefaclor were monitored for 8 hours after oral drug administration. AUC, the area under the plasma concentration-time curve from time zero to 8 hr (13 points), was calculated by the linear trapezoidal rule method. C,,,a" (maximum plasma drug concentration) and Tmax (time to reach Cma,) were compiled from the plasma concentration-time data. Analysis of variance was carried out using logarithmically transformed AUC, and Cmax. No significant sequence effect was found for all of the bioavailability parameters indicating that the cross-over design was properly performed. The 90% confidence intervals of the AUC, ratio and the Cmax ratio for Neocer/Ceclor" were 0.9049 S S < 1.0304 and 0.9776 5 S 1.226, respectively. These values were within the acceptable bioequivalence intervals of 0.80-1.25. Thus, our study demonstrated the bioequivalence of Neocef"/Ceclor" with respect to the extent of absorption.

가용화 조성물과 난용성 약물군을 함유하는 고체분산체의 용출양상

김태완,최춘영,Cao, Qing-Ri,권경애,이범진 한국약제학회 2002 Journal of Pharmaceutical Investigation Vol.32 No.3

Polymer based physical mixtures or solid dispersions containing solubilizing compositions[OA, tween80 and SLS] were prepared using a spray-dryer. Lovastatin(LOS), simvastatin(SIMS), aceclofenac(AFC) and cisapride(CSP) were selected as poorly water-soluble drugs. Dextrin, poly(vinylalcohol)(PVA), poly(vinylpyrrolidone)(PVP) and polyethylene glycol(PEG) were chosen as solubilizing carriers for solid dispersions. The solid dispersions containing solubilizing compositions without drug were prepared without using organic solvents or tedious changes of formulation compositions. This system could be used to quickly screen the dissolution profiels of poorly water-soluble drugs by simply mixing with drugs thereafter. In case of solid dispersion containing drug, organic solvent systems could be used to solubilize model drugs. The dissolution rates of the drugs were higher when mixed with drug and solid dispersions containing solubilizing compositions. However, solid dispersions of LOS, AFC, CSP simultaneously containing drug and solubilizing compositions in organic solvent systems were more useful than physical mixtures of drug and solid dispersions without drug except SIMS. Based on solubilizing capability of polymer based physical mixtures in gelatin hard capsules, optimal solid dispersion system of poorly water-soluble drugs could be formulated. However, it should be noted that dissolution rate of poorly water-soluble drugs were highly dependent on drug properties, solubilizing compositions and polymeric carriers.

가용화 조성물을 함유한 PVP형 고체분산체의 제조 및 특성 : Based Solid Dispersion Systems Containing Solubilizers

Cao, Qing-Ri,김태완,최춘영,권경애,이범진 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.1

The PVP-based solid dispersion systems (SDs) containing lovastatin (LOS) and solubilizers (sodium lauryl sulfate, tween 80 and oleic acid) were prepared to enhance dissolution rate of practically water insoluble LOS using solvent evaporation method. Two different organic cosolvents either acetone/ethanol or acetonitrile/ethanol were used for the preparation of SDs. The LOS contents were highly decreased when acetone/ethanol cosolvents were used. The decrease of LOS contents was not caused by acetonitrile or acetone, based on HPLC data. The surface morphology as investigated by scanning electron microscope (SEM) and angle of repose as an index of flowability of SDs were highly dependent of the type and amount of solubilizers used. Base on differential scanning calorimetry (DSC) and X-ray powder diffraction data, the SDs made crystalline LOS into amorphous structure or partially eutectic mixtures. The simultaneous use of the solubilizers in SDs was also useful to increase dissolution rate of LOS in gastric or intestinal fluid. The SDs containing solubilizers reached 76% and 60% in gastric and intestinal fluid, respectively but the commercial tablet gave only less that 4%. These solubilizers in SDs could be also applicable for enhancing dissolution and bioavailability of poorly water-soluble drugs.

조직공정성과 조직후원인식의 관계에 관한 연구

정범구,김경재 충남대학교 경상대학부설 경영경제연구소 1997 경영논집 Vol.13 No.2

본 연구는 조직공정성과 조직후원인식의 상호관계를 알아보기 위해서 조직공정성과 조직후원인식의 개념과 이론을 정리하였고 연구모형과 가설을 통해서 실증분석결과를 제시하였다. 연구결과 조직공정성은 조직후원인식에 정적인 영향을 미치는 것으로 나타났고 조직공정성이 종속변수에 대하여 미치는 직접효과보다 조직후원인식을 매개로 하였을 때의 매개효과가 더 큰 것으로 나타났다.

급성 지주막하 출혈에 동반된 관상동맥 연축에 의한 심근경색증 1예

부귀범,박근호,양종태,이동민,장성종,김건영,장경식 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

In many cases, acute cerebrovascular disease is accompanied by abnormal electocardiograms. Also, though rather uncommon, acute myocardial infraction is also seen. The mechanism of its occurance is uncertain but it is thought to be related to coronary artery spasms, an abnormal autonomic nervous system, and a catecholamine increase. A female patient of 34 years of age came to the hospital because of sudden headache accompanied by substernal chest pain. A subarachnoid hemorrhage due to a cerebral aneurysm rupture had occured, and there was ST segment elevation in lead II, III, and aVF of the electrocardiogram. A cardiac enzyme test, an echocardiography, and a coronary angiography were performed, and she was diagnosed to have acute myocardial infarction due to coronary artery spasm. Clipping of the aneurysm was performed. With the use of nitrate and an angiotension converting enzyme inhibitor, the condition of the patient improved.

뇌실내출혈로 인한 급성 수두증에서 관찰된 해리증후군

김광기,이경민,이정주,오종배,안태범,정상욱,남현우,박성호 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5