실험적 급성 허혈성 신부전에서 Verapamil 이 혈중 Polyamine 동태에 미치는 영향

원동준(Dong Jun Won),권현민(Hyun Min Kwon),김용섭(Yong Seop Kim),구자룡(Ja Ryong Gu),권영주(Young Ju Kwon),조원용(Won Yong Cho),김형규(Hyung Kyu Kim) 대한내과학회 1991 대한내과학회지 Vol.40 No.6

N/A It has been proposd that calcium entry from an external medium increases intracellular free calcium to toxic levels during ischemic acute renal failure, and verapamil (ARF) has been suggested as the agentblocking calicium entry into renal cells and protecting renal function during ischemic injury. Polyamines, anorganic cations that play various roles in normal cellular proliferation and differentiation, accumulate in renal failare. Alsa it has been suggested tht the synthesis and metabolism of polyamine are influence by acute changes of the general condition, such as acute renal failure, and are mediated by a calcium influx into the cells. The study was designed to study the protective effects of systemic verapamil pretreatment on renal function and the influence on polyamine metabolism in experimental ischemic ARF in cats. For these purpose, the experimental animals were divided into 2 groups. While Group I (n=5) was an ischemic ARF model by renal artery clamping for 60 minutes, Group II (n=5) was ischemic ARF with systemic verapamil (5 ml/min/kg) pretreatment. The creatinine clearance and plasma and urinary polyamine were measured in each group before and after the renal artery clamp. The results were as follows: 1) Creatinine clearance before and after the renal artery clamp were 10.64±7.18 ml/min/kg and 2.09±1. 70 ml/min/kg in Group I, 4.47±3.38 ml min/kg and 0.60±0.79ml/min/kg in Group II, respectively, So creatinine clearance decreased more significantly in Group II campared with Group I. 2) Plasma polyamine increased after ischemia in Group I. In group I, plasma levels of putrescine, spermidine, and spermine before ischemia were 4.75±0.40 nmol/ml, 0.69±0.09 nmol/ml, and 0.83±0.63 nmol/ml, were elevated to 7.17±2.91 nmol/ml, 9.83±1.46 nmol/ ml, and 2.64±1.14nmol/ml after ischemia. But in Group II, the plasma level of polyamine was not changed, and especially, spermine decreased significantly from 0.83±0.27 before ischemia to 0.49±0.23 nmol/ml after ischenmine (p=0.033). 3) Urine polyamine excretion decreased after ischemia in Group I and Group II. In Group II, urinary excretion of spermidine and spermine before ischemia, 0.13±0.10 nmol/min and 0.17±0.13nmol/min, decreased after ischemia to 0.01±0.01nmol/min (p=0.019) and 0.032±0.26 nmol/min (p=0.0257). 4) In renal tissue, spermine content vas highest. In Group II, preischemic spermine were 397.20 nmol/g and increased to 646.66nmol/g after ischema, But there were no significant changes in the polyamine contents in Group II. From these data, it was suggested that systemic verapamil pretreatment exerts no protective effect on ischemic ARF. Plasma polyamines are elevated in ischemic ARF, and verapamil may protect these elevations.

유방암의 위와 대장전이

유현아,김은영,서민지,정은,조민정,오현진,장지혜,박지찬,이정의,박석영 이화여자대학교 의과학연구소 2014 EMJ (Ewha medical journal) Vol.37 No.2

Gastric metastasis from breast cancer is rare and only six cases have been reported in Korea. Colon metastasis is more rare than gastric metastasis. We report a 63-year-old woman with gastric and colon metastases of invasive lobular carcinoma of breast. She was diagnosed as right breast cancer, received right modified radical mastectomy 10 years ago and has been treated with chemotherapy and hormone therapy. Investigating for melena and a small caliber of stool, we found gastric and colon metastases. The diagnosis of metastatic breast cancer was made through gross pathologic and immunohistochemistry staining. We report a case with gastric and colon metastases from breast cancer and a review of the associated six case reports in Korea.

The Korean Association of lnternal Medicine : Poster Session ; PS 1357 : Nephrology ; Age is the Strongest Effector for the Relationship between Estimated Glomerular filtration Rate and Coronary Artery Calcifi cation in Apparently Healthy Korean Adults

( Hyun Beom Chae ),( Eun Jung Rhee ),( Hae Jung Park ),( Min Kyung Lee ),( Won Seon Jeon ),( Se Eun Park ),( Cheol Young Park ),( Won Young Lee ),( Ki Won Oh ),( Sung Woo Park ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Cardiovascular disease (CVD) is a major clinical problem and the most common cause of morbidity and mortality in patients with chronic kidney disease (CKD). Coronary artery calcifi cation (CAC) is a potential mechanism that explains the association between renal function and cardiovascular mortality. We aimed to evaluate the association between renal function and CAC in apparently healthy Korean subjects. Methods: In 23,617 participants in the health screening program in Kangbuk Samsung Hospital health screening program from January 2010 to December 2011, estimated glomerular fi ltration rate (eGFR) was assessed by Cockcroft-Gault equation. Coronary artery calcium score (CACS) was measured by multi-detector computed tomography (MDCT). Subjects were divided into 3 groups according to the CKD Staging system with eGFR grade; stage 1: eGFR = 90 mL/min/1.73 m2, stage 2: eGFR 60-89 mL/ min/1.73 m2, stage 3: eGFR 30-59 mL/min/1.73 m2. Results: Mean age of the participants was 41.4 years, and mean eGFR was 103.6±21.7 mL/min/1.73 m2. Hypertension and diabetes were noted in 43.7% and 5.5% of the participants, respectively. eGFR showed weakly negative but signifi cant association with CACS in bivariate correlation analysis (r=-0.076, p<0.01). Mean CACS signifi cantly increased from CKD stage 1 to 3. The proportion of subjects who had CAC signifi cantly increased from CKD stage 1 to 3. Although odds ratio for CAC signifi cantly increased from stage 1 to 3 after adjustment for confounding factors, this signifi cance was inversed when age was included in the model. Age was the strongest factor that affects CAC. Conclusions: This study performed in young Korean subjects with relatively normal renal function, age was the mostly strongest effector on the association between eGFR and the degree of CAC.

Optical properties of hydrogel contact lens material containing 1-vinylimidazole

Min-Jae Lee,Dong-Hyun Kim,A-Young Sung 대한시과학회 2014 대한시과학회지 학술대회 Vol.2014 No.05

Purpose: This study evaluated the physical and optical characteristics of hydrophilic contact lens polymerized with addition of 1-vinylimidazole in the basic hydrogel contact lens material, and in particular, the utility of 1-vinylimidazole as a contact lens material for ophthalmologic devices was investigated. Methods: This study used 1-vinylimidazole with the cross-linker EGDMA (ethylene glycol dimethacrylate), HEMA (2-hydroxyethyl methacrylate) and the initiator AIBN (azobisisobutyronitrile) for copolymerization. The polymerization of the contact lens material was conducted through thermal polymerization in 100°C for about 40 mins. To produce the high functional contact lenses, a cast mould method was used. Results: The physical properties of the hydrogel ophthalmic material were investigated by measuring the average value of refractive index, water content and optical transmittance. The refractive index of 1.4330~1.4408, water content of 36.10~38.59%, UV-A transmittance of 90.0~72.2% and UV-B transmittance of 88.6%~64.2% and visible ray transmittance of 89.2~91.8% were obtained. The transmittance for visible light was over 89% in all combinations with 1-vinylimidazole and the transmittance for UV reduced up to a certain level in certain combinations with 1-vinylimidazole. Conclusions: Based on the results of this study, the produced copolymer can be estimated to be suitable for use as ophthalmic lens material for UV- blocking effects.

급성 파라쿼트 중독에서 혈중 파라쿼트 농도

박승민,김세현,최수진,김현,이완구,김영남,이광영,이영희,신성혜 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background: Paraquat is a bipyridyl compound, and when ingested, concentrated paraquat can cause either rapid death from multisystem failure and cardiovascular shock or delayed death from progressive pulmonary fibrosis. Paraquat is poorly absorbed by inhalation, but when ingested orally, severe illness can occur. Death usually occurs within 2 days if more than 50 ㎎/㎏ of paraquat is ingested . The most important prognostic indicator is the quantity of paraquat absorbed, as shown by the plasma paraquat concentration, and the prognostic indication depends mostly on the description given by the patients and their families about the amount of paraquat ingested, which is often underestimated or overestimagted. For these reasons, we tried to compare the plasma paraquat concentrations with amount of paraquat described by patients or their families. Methods: We reviewed the medical records of 59 patients with acute paraquat poisoning from February 1998 through February 1999, the paraquat concentrations in plasma were measured at Presbyterian Medical Center by using high performance liquid chromatography. Results: There was a striking discrepancy between the plasma paraquat concentration and the ingested amount described by the patients or their families. Conclusion: We recommend that the plasma paraquat concentration be measured in patients being treated for acute paraquat poisoning.

인터넷 중독 청소년들의 정신병리 및 대처 방식

안영균(Young Kyun Ahn),신영철(Young Chul Shin),고복자(Bok Ja Koh),심진현(Jin Hyun Shim),김범조(Bum Jo Kim),이승민(Seung Min Lee),이길홍(Kil Hong Lee) 한국중독정신의학회 2005 중독정신의학 Vol.9 No.1

This study was conducted to investigate the differences in psychopathology and coping strategies among internet addictors, over users and non addictors in high school students. Subjects were 273 high school students in Seoul city. Self rating questionnaires included demographic data, Young’s Internet Addiction Scale, SCL-90-R, Beck Depression Inventory, Spielberger State-Trait Anxiety Inventory, and The Way of Coping Strategy. Subjects were classified into 3 groups (addiction, over use, non addiction) by Internet Addiction Scale score. Prevalence of internet addiction was 4.3% and male adolescents were more addictive than female. Students who were mainly involved with internet chatting or game showed higher internet addiction score. The addiction group and over use group showed higher level of somatization, obsession, interpersonal sensitivity, depression, anxiety, hostility, and paranoia than non-addicted group. Addiction group and over use group used passive coping strategies more frequently in the stressful situation than nonaddicted group. Also, the regression analysis for finding out causal factors that predicts internet addiction showed that emotion relieving strategy were important variable which predict internet addiction. Interpersonal sensitivity and emotion relieving coping strategy was important to predict addiction. This study showed that problematic internet use was closely associated with psychopathology and psychiatric comorbidity. And high interpersonal sensitivity and emotion relieving coping strategy were related with internet addiction in adolescence.

Comparing hemostatic resuscitation management of intraoperative massive bleeding with traumatic massive bleeding: a computer simulation

Lee Young Sun,Kim Kyu Nam,Lee Min Kyu,Sun Jung Eun,Hyun Jin Lim,전종헌 대한마취통증의학회 2020 Anesthesia and pain medicine Vol.15 No.4

Background: Appropriate blood component transfusion might differ between intraoperative massive bleeding and traumatic massive bleeding in the emergency department because trauma patients initially bleed undiluted blood and replacement typically lags behind blood loss. We compared these two blood loss scenarios, intraoperative and traumatic, using a computer simulation. Methods: We modified the multi-compartment dynamic model developed by Hirshberg and implemented it using STELLA 9.0. In this model, blood pressure changes as blood volume fluctuates as bleeding rate and transcapillary refill rate are controlled by blood pressure. Using this simulation, we compared the intraoperative bleeding scenario with the traumatic bleeding scenario. In both scenarios, patients started to bleed at a rate of 50 ml/min. In the intraoperative bleeding scenario, fluid was administered to maintain isovolemic status; however, in the traumatic bleeding scenario, no fluid was supplied for up to 30 min and no blood was supplied for up to 50 min. Each unit of packed red blood cells (PRBC) was given when the hematocrit decreased to 27%, fresh frozen plasma (FFP) was transfused when plasma was diluted to 30%, and platelet concentrate (PC) was transfused when platelet count became 50,000/ml. Results: In both scenarios, the appropriate ratio of PRBC:FFP was 1:0.47 before PC transfusion, and the ratio of PRBC:FFP:platelets was 1:0.35:0.39 after initiation of PC transfusion. Conclusion: The ratio of transfused blood component did not differ between the intraoperative bleeding and traumatic bleeding scenarios.

벤조디아제핀 수용체 영상용 양전자 방출 핵종 표지 플루마제닐 유도체 [F-18](3-(2-Fluoro)flumazenil의 합성과 생체 내 분포

장영수,이숙자,강삼식,홍성현,이명철,이동수,정준기,정재민,조정혁 대한핵의학회 1999 핵의학 분자영상 Vol.33 No.6

Purpose: Radiotracers that bind to the central benzodiazepine receptor are useful for the investigation of various neurological and psychiatric diseases. [C-11]Flumazenil, a benzodiazepine antagonist, is the most widely used radioligand for central benzodiazepine receptor imaging by PET. We synthesized 3-(2-[F- 18]fluoro)flumazenil, a new fluorine-18 (t1/2=110 min) labeled analogue of benzodiazepine receptor imaging agent, and evaluated in vivo for biodistribution in mice. Materials and Methods: Flumazenil (Ro 15-1788) was synthesized by a modification of the reported method. Precursor of 3-(2-[F-18]fluoro)flumazenil, the tosylated flumazenil derivative was prepared by the tosylation of the ethyl ester by ditosylethane. [F-18] labeling of tosyl substitued flumazenil precursor was performed by adding F-18 ion at 85℃ in the hot cell for 20 min. The reaction mixture was trapped by C18 cartridge, washed with 10% ethanol, and eluted by 40% ethanol. Bidistribution in mice was determined after intravenous injection Results: The total chemical yield of tosylated flumazenil derivative was ∼40%. The efficiency of labeling 3-(2-[F-18]fluoro)flumazenil was 66% with a total synthesis time of 50 min. Brain uptakes of 3-(2-[F-18]fluoro)flumazenil at 10, 30, 60 min after injection, were 2.5±0.37, 2.2±0.26, 2.1±0.11 and blood activities were 3.7±0.43, 3.3±0.07, 3.3±0.09%ID/g, respectively. Conclusion: We synthesized a tosylated flumazenil derivative which was successfully labeled with no-carrier-added F-18 by nucleophilic substitution.

금 박막의 기계적 물성에 끼치는 두께와 미세조직의 영향

전영선(Young-Sun Jeon),이상주(Sang-Joo Lee),현승민(Seung-Min Hyun),이학주(Hak-Joo Lee) 대한기계학회 2007 대한기계학회 춘추학술대회 Vol.2007 No.10

We have used Visual Image Tracing(VIT) strain measurement system coupled with a micro tensile testing unit to test thickness and microstructure dependent mechanical behavior of Au film. The advantage using this method is to monitor the real time images of specimen during the test in order to determine its Young’s modulus, yield strength and tensile strength. 0.5, 1 and 2 ㎛ thick films were deposited by sputtering system. Free standing films were prepared through lithography and etching processes. Au films were annealed at 300℃ and 350℃ to achieve microstructure evolution and Focus ion beam (FIB SEM) and transmission electron microscopy (TEM) were employed to characterize grain sizes of films. Thickness and grain sizes dependent mechanical behavior such as stress-strain curve, Young’s modulus and yield strength of Au thin film will be presented.

설문지를 이용한 신체활동량과 참여강도가 중년여성의 체력 및 관상동맥 위험인자에 미치는 영향

안영환(Young Hwan An),박세정(Saejong Park),최현민(Hyun Min Choi),김종경(Jong Kyung Kim),노호성(Hosung Nho) 한국발육발달학회 2015 한국발육발달학회지 Vol.23 No.1

The purpose of the study was to investigate the effects of the volume of physical activity on obesity, physical fitness and coronary risk factors among the middle-aged women. physical activity was assessed from International Physical Activity Questionnaire (IPAQ) and were categorized by health enhancing physical activity group (PG), minimum activity group (MG), and non-activity group (NG) according to the volume of physical activity. Apparently healthy middle-aged women (n=163; 52.1±8.6 yrs) participated and they were classified as PG (n=46; 5,878±3547 MET·min/week), MG (n=64; 1,678±886 MET·min/week), and NG (n=52; 456±389 MET·min/week). Also, method of the study was categorized by vigorous activity and moderate activity according to the study of WHO (2010) and Mikael Fogeholm (2006). Measurement variables were body composition, physical fitness (hand-grip, sit-up, pacer, sit & reach, shuttle run, and long jump), total cholesterol (TC), low-density lipoprotein cholesterol (LDLC), high-density lipoprotein cholesterol (HDLC), and triglycerides (TG). One-way ANOVA was used with Tukey post-hoc (p<.05). In the result, PG had significantly low percent body fat comparing with MG or NG (p=.02). And, among physical fitness variables flexibility was higher in PG than in MG (p=.03). While HDLC (p=.007) was higher in the PG than MG or NG, LDLC (p=.024) was lower in the PG than MG. TG (p=.021) was lower in the PG than MG or NG. Vigorous activity group had significantly high pacer in classification as physical activity intensity. Glucose was lower in vigorous activity group than in moderate activity group (p=.02). In conclusion, it was generally low body fat rate and coronary risk factors for middle-aged women to increased volume of physical activity of daily life and participating vigorous activity indicated that was able to keep a high fitness standard.