Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

초등교사의 열정에 대한 인식: 의미연결망 분석의 활용

임효진 ( Hyo Jin¸ Lim ),최은혜 ( Eun Hye¸ Choi ),박호익 ( Ho Ik¸ Park ),이지현 ( Ji Hyun¸ Lee ) 한국초등교육학회 2021 초등교육연구 Vol.34 No.3

본 연구에서는 초등교사가 가지고 있는 열정에 대한 인식구조를 파악하기 위해 현직 초등교사 131명을 대상으로 설문조사를 실시하였다, 연구에서는 열정, 열정의 장단점, 교사열정에 대한 인식구조를 알아보기 위해 의미연결망 분석을 활용하여 근접 중심성, 연결중심성, 매개중심성, 아이겐벡터 중심성을 위주로 살펴보았다. 연구결과 첫째, 열정에서는 ‘힘’,‘강렬’과 같은 높은 강도나 에너지를 나타내는 단어들이 중요한 역할을 하였으며 커뮤니티 분석 결과 열정의 행동, 정서, 동기 특성에 대한 주제가 분류되었다. 둘째, 열정의 장점에서는 ‘삶’,‘원동력’,‘에너지’등이, 열정의 단점에서는 ‘타인’,‘피로’,‘살피지 못함’등이 중요한 역할을 하는 것으로 보아 기존 이론에 나타난 열정의 특성을 설명하고 있었다. 셋째, 교사열정에서는 ‘학생’,‘수업’과 같은 교수활동의 실천을 나타내는 단어들이 중요한 역할을 하였으며 커뮤니티 분석 결과 교육활동 전반, 학생과 수업, 전문성 개발에 대한 주제가 분류되었다. The purpose of this study was to explore the meaning of passion perceived by elementary school teachers. In order to understand the structure of perception of passion, we surveyed 131 in-service elementary school teachers. Responses to passion, the positive and negative role of passion, and teacher passion were analyzed using a semantic network analysis. We focused on closeness centrality, degree centrality, betweenness centrality and eigenvector centrality. Results showed that first, in passion, words indicating high strength and energy such as‘power’and‘intensity’played an important role in the structure of perception. Themes on behavioral, emotional, and motivational characteristics of passion were classified as sub-factors of passion as a result of community analysis. Secondly,‘life’,‘motivation’, and‘energy’appeared in key-word analysis in the positive role of passion while‘others’,‘fatigue’, and‘not caring for’appeared in the negative role of passion, reflecting the theory on the dualistic model of harmonious and obsessive passion. Third, in teacher passion, words indicating teaching activities and their practices such as‘student’and‘instruction’played an important role in the structure of perception. Themes related to educational activities in general, students and instruction, and professional development were classified as sub-factors of teacher passion.

북미자유무역협정(NAFTA)의 출범과 미국노사관계의 변화

임현진,최종철,홍성태 서울대학교 국제지역원 1996 국제지역연구 Vol.5 No.4

탈냉전 시기 미국의 새로운 국제전략은 세계 각국의 불균등한 발전상황을 적절히 이끌어 새로운 팍스 아메리카나의 시대를 열겠다는 명확한 목표를 가지고 있다. 기술 및 산업의 차원에서 이 목표는 정보통신분야의 주도권 장악으로 추진되고 있으며, 세계무역질서의 차원에서는 WTO와 NAFTA의 구축으로 젼개되고 있다. 여기서 NAFTA는 두가지 함을 갖는다. 첫째, 궁극적으로 지구적 지배력을 재구축하기 위한 지역적 발판을 마련하는 것이다. 둘째, 발전도상국과 선진국의 전면적 자유무역이 개신된다는 것이다. 미국 노사관계의 변화와 관련해서는 특히 두 번째 측면에 주목할 필요가 있을 것이다. 그러나 현재 진행되고 있는 미국 노사관계의 변화는 NAFTA 자체보다는 포드주의의 위기와 다른 선진자본주의국과의 경쟁에서 더 큰 영향을 받고 있는 것으로 판단된다. 즉 미국적 노사관계를 대표해 온 뉴딜형 노사관계와 실리적 조합주의의 한계에 대한 인식은 바로 이것들이 기반하고 있는 포드주의에 대한 비판과 대체의 과정에서 필연적으로 나타나고 있는 것이다. 이와 관련하여 NAFTA는 미국의 노동조합이 종래의 보호주의적 외투를 벗어버리고 개발도상국 노동자들과의 연대를 적극적으로 추구해야 할 필요성을 강화한다. 포스트 포드주의를 배경으로 미국의 자본과 정부가 주도하고 있는 실리적 조합주의에서 참여적 조합주의로의 변화에 대해 미국의 조동조합이 얼마나 적극적으로 대처할 수 있느냐의 문제는 새로운 국제적 연대에 얼마나 적극적으로 대처할 수 있느냐의 문제와 깊은 상관관계를 가질 것이다. The international strategies of America in the post cold-war period have the apparent objective that America will open a new pax-Americana age, by leading the unequal developmental situations among every countries in the world. This objective is propelled through holding the initiative in the field of information and communication in terms of technology and industry, and is developed by the establishment of the WTO and NAFTA in terms of the world trade order. Here, NAFTA has two implications; first, preparing for a regional platform so that America will be able to re-establish its global power; secon, the overall free-trade between advanced countries and developingcountries is beginning. With regardto the change of America's capital-labour relations, we need to call our attention to the second aspect. But it seems that the change of America's capital-labour relations in progress is more affected by the competition with other advanced countries than by NAFTA. That is, the recognition of the limits of the New-Deal capital-labour relations up to the present is necessarily emerging in the process of cirticizing and replacing Fordism which is regarded as a basis for both. Accordingly, NAFTA is strengthening its position on the necessity for labour in the US to actively pursue solidarity with unions in developing countries, taking off its old protectionist cloat. As for unions in America, how to cope with the transition from business unionism to participatory unionism which America's capital and government are leading in the back-ground of post-Fordism will be closely related with how actively to pursue a new international solidarity.

인터넷을 활용한 진로정보탐색 프로그램이 대학생의 진로 자기효능감에 미치는 영향

임은미,김현진 전주대학교 사회과학종합연구소 2002 社會科學論叢 Vol.18 No.-

본 연구는 인터넷을 활용한 진로정보탐색 프로그램이 대학생들의 진로자기효능감에 미치는 영향을 검증하기 위한 것이다. 이에 대학생을 대상으로 하는 인터넷을 활용한 진로정보탐색 프로그램을 제작하였다. 이 프로그램은 김봉환 등(2001)이 제안한 모듈식 진로상담 프로그램의 개념을 임은미와 장선숙(2002)이 중등학생을 위해 적용한 것을 대학생의 상황에 맞게 수정하여 제작되었다. 프로그램 회기는 4회기로 구성되어 있으며, 그 내용은 프로그램 분위기 조성 및 온라인 직업흥미검사 실시(60분), 직업흥미검사 결과의 해석과 직업세계의 이해(60분), 정보사냥대회(60분), 직업정보탐색과 마무리로 이루어져 있다. 프로그램의 실시 전후에 <진로의사결정 자기효능감 검사>를 실시하고, 사전-사후검사 점수 차이를 분석하여 프로그램의 효과를 검증하였다. J대학교의 1학년 남녀학생 가운데 자발적인 신청자들을 대상으로 본 프로그램을 적용한 후 실험집단과 통제집단의 사후검사 점수 차이를 비교한 결과 프로그램 참가자들은 통제집단에 비하여 사후검사에서 의미있게 높은 점수를 얻었다. 이러한 경향은 진로 의사결정 전체 Q뿐 아니라 문제해결 하위영역을 제외한 목표설정, 계획수립, 자기평가, 직업정보탐색의 전 하위 영역에서도 동일하게 나타났다. 이러한 결과는 대학생을 대상으로 인터넷을 활용하여 단기에 실시하는 진로정보탐색 프로그램이 대학생의 진로의사결정 효능감을 높여줄 수 있음을 시사한다. The purpose of this study is to examine the effects of career information exploration program using internet on the undergraduates' career decision-making self-efficacy. For this purpose, career information exploration program using internet was developed. The main factors of this program are performing the free on-line interest test, explorating the career information web-sites, and getting career information through internet according to the participants' interests. The results showed that the experimental group got higher score than the control group in CDMSES(Career Decision-Making Self-Efficacy Inventory). But for the problem solving subcategory, this tendency was shown in all the sub factors of CDMSES, i.e. goal-setting, career planning, collecting information, and self -evaluation. The implications about education or counseling programs for the undergrauates were discussed.

기기 중성자방사화분석법을 이용한 황사특성

임종명,이현석,이진홍 忠南大學校 環境問題硏究所 2002 環境硏究 Vol.20 No.-

Using INAA, which has been thought to be one of the best analytical techniques for airborne particulate matters, this study centered on the quantitative analysis of 32 metal elements within Asian Dust aerosols. Concentrations of REEs of crustal origin, such as Ce, Cs, Dy, La, Sc, and Sm were more than 8.5 times higher in Asian Dust aerosols. In addition, major crustal elements, such as Al, Ca, Co, Fe, K, Mg, Mn, and Ti showed more than 3 times higher concentrations in those aerosols, which made PM 10 and all metal concentrations be also about 3.0 and 4.8 times higher, respectively. However, concentrations of anthropogenic origin, such as As, Br, Ci, I, In, Sb, Se, and Zn were increased to be a little higher, which means no great effect of Asian Dust on these elements.

7β-Hydroxycholesterol이 사람 백혈암에 대한 세포 독성 효과

임희경,현진원 제주대학교 생명과학기술혁신센터 2002 제주생명과학연구 Vol.5 No.-

The cytotoxic activity of 7β-hydroxycholesterol (7β-OHC) has been evaluated using various leukemia cell lines by MTT test. Among the tested cell lines, 7β-OHC showed to be more cytotoxic to THP-1, human monocytic leukemia cell line. IC50 value of 7β-OHC on THP-1 was 10μM. Kinetic of on THP-1 showed that growth of THP-1 began to decrease at 36 h after treatment 7β-OHC. 7β-OHC induced apoptosis shown by DNA fragmentation by gel electrophoresis. 7β-OHC induced apoptosis through down-regulation of Bcl-2 expression. Taken together, these results suggested that treatment with 7β-OHC inhibits the proliferation of THP-1 cells through apoptosis.

리스페리돈과 올란자핀이 투여된 정신과 환자들에서 간 효소 변화에 대한 후?적 연구

임현국,배치운,박석준,김정진,이창욱,이수정,이철,백인호 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.3

EDF 기대손실에 기초한 로버스트 공정능력지수

임태진,송현석 한국품질경영학회 2003 품질경영학회지 Vol.31 No.1

This paper presents a robust process capability index(PCI) based on the expected loss derived from the empirical distribution function(EDF). We propose the EDF expected loss in order to develop a PCI that does not depends on the underlying process distribution. The EDF expected loss depends only on the sample data, so the PCI based on it is robust and it does nor require complex calculations. The inverted normal loss function(INLF) is employed in order to overcome the drawback of the quadratic loss which may increase unboundedly outside the specification limits. A comprehensive simulation study was performed under various process distributions, in order to compare the accuracy and the precision of the proposed PCI with those of the PCI based on the expected loss derived from the normal distribution. The proposed PCI turned out to be more accurate than the normal PCI in most cases, especially when the process distribution has high kurtosis or skewness. It is expected that the proposed PCI can be utilized in real processes where the true distribution family may not be knwon.

EDF 기대손실에 기초한 공정능력지수의 붓스트랩 신뢰구간

임태진,송현석 한국품질경영학회 2003 품질경영학회지 Vol.31 No.4

This paper investigates bootstrap confidence intervals of the process capability index(PCI1 based on the expected loss derived from the empirical distribution function(EDF1. The PCI based on the expected loss is too complex to derive its confidence interval analytically, so the bootstrap method is a good alternative. We propose three types of the bootstrap confidence interval; the standard bootstrap(SB1, the percentile bootstrap(PB1, and the acceleration biased-corrected percentile bootstrap(ABC1. We also perform a comprehensive simulation study under various process distributions, in order to compare the accuracy of the coverage probability of the bootstrap confidence intervals. In most cases, the coverage probabilities of the bootstrap confidence intervals from the EDF PCI turned out to be more accurate than those from the PCI based on the normal distribution. It is expected that the bootstrap confidence intervals from the EDF PCI can be utilized in real processes where the true distribution family may not be known.

3-D 동작 분석 장치를 이용한 한국성인(20대, 30대)의 동작 범위에 관한 연구

임현균,김진호,박수찬,류신아,이영신 한국운동역학회 1998 한국운동역학회지 Vol.8 No.2

Devices, such as a goniometer, have been used to measure a human being's active range of motion, but interferences between subjects and testers have affected the result precision. In this study, 56 dynamic motion ranges were measured using a 3-D motion analysis system which is quite accurate (2mm error for 1m). The 3-D motion analysis system consists of VICON 140, data acquisition system, and data analysis program (KRISSMAS). 80 Korean subjects (40 males, 28±6.1 aged and 40 females, 28.4±6.9 aged) participated. Subjects were divided into 4 groups by Ro¨hrer's index based on National Anthropometric Survey of Korea 1997. Basic statistical analysis were performed using SPSS (ver. 8.0) programs after refining data. Various comparisons were performed for the right and left side, male and female, age groups (20's, 30's. and 40's-60's) using previous studies. The results show that female subjects have more flexible joints in the neck, hip, ankle, and wrist, while male subjects have more flexibility in their waist. But most of the significances were not high (>0.05). The prediction that the right side of Korean bodies would be mort flexible was not a good hypothesis. And the joints flexibilities are not correlated with Ro¨hrer's Index.