Platycodon grandiflorum root-derived saponins attenuate atopic dermatitis-like skin lesions via suppression of NF-kB and STAT1 and activation of Nrf2/ARE-mediated heme oxygenase-1

( Jae Ho Choi ),( Sun Woo Jin ),( Eun Hee Han ),( Bong Hwan Park ),( Hyung Gyun Kim ),( Tilak Khanal ),( Yong Pil Hwang ),( Minh Truong Do ),( Hyun Sun Lee ),( Young Chul Chung ),( Hee Suk Kim ),( Tae 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

Purpose: The consequences of precipitously rising allergic skin inflammation rates worldwide have accelerated the risk of atopic dermatitis (AD). Natural product-based agents with good efficacy and low risk of side effects offer promising prevention and treatment strategies for inflammation-related diseases. We have already reported that Platycodon grandiflorum root-derived saponins (Changkil saponins, CKS) have many pharmacological effects, including anti-inflammatory and anti-allergic effects, but its influence on AD remains unclear. Therefore, we evaluated the inhibitory effect of CKS, mainly platycodin D, on AD-like skin symptoms in mice and the possible mechanisms in cells. Methods: Mice were sensitized and challenged with 2,4-dinitrochlorobenzene (DNCB). Four weeks after challenge, mice were treated with oral administration of CKS for 4 weeks. In addition, cells were used to evaluate the effect of CKS, mainly platycodin D, on the TARC expression regulated mechanism. Results: CKS attenuated DNCB-induced dermatitis severity, serum levels of IgE and TARC, and mRNA expression of TARC, TNF-α, IFN-γ, IL-4, IL-5, and IL-13 in mice. Histopathological examination showed reduced thickness of the epidermis/dermis and dermal infiltration of inflammatory cells and mast cells in the ears. Moreover, CKS and platycodin D inhibited TNF-α/IFN-γ-induced TARC expression through the suppression of NF-κB and STAT1 and induction of Nrf2/ARE-mediated hemeoxygenase-1 (HO-1) expression in cells. Conclusion: We suggest that CKS and platycodin D inhibited the development of AD-like skin symptoms by regulating cytokine mediators and may be an effective alternative therapy for AD-like skin symptoms.ⓒ2014 Elsevler Gmbh. All rights reserved.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

혈액투석 환자의 가성 고칼륨혈증

최창렬,조형도,강태영,이준수,한호,정청일,이동규,유준호,한상웅,김호중 대한신장학회 2001 대한신장학회잡지 Vol.20 No.5

현미 온도 조질 후 정백 특성

한충수,김유호,강태환,조성찬 한국산업식품공학회 2003 산업 식품공학 Vol.7 No.1

겨울철 곡온이 낮은 현미는 조직이 단단해져서 정맥시간이 길어지고, 과부하로 인해 도정효율 저하와 소비전력량이 증가한다. 조질은 곡물의 함수율과 곡온을 조절하는 기술로써, 현미의 단단한 조도정수율 및 도정효율을 향상시킬 수 있다. 본 연구에서는 저온 현미 정백시 도정효율 향상 및 품질유지를 위해 적정 현미 조질온도와 정백 후 백도, 강도, 동할미율, 싸라기율, 곡온 상승, 함수율 변화, 소비전력량 등과의 관계를 규명하였다. 현미 조질 곡은 0, 3, 5, 12, 20℃에 대한 정백후 백미 백도 변화는 큰 차이가 없는 것으로 나타났고, 동할미율, 완전립과 동할립 강도 및 싸라기율은 현미 조질 곡온이 높을수록 감소하는 것으로 나타났다. 조질 곡은 12, 20℃인 경우가 정백 후 적정 곡온 상승 규정범위 15℃ 내에 해당되었다. 정백 후 함수율 변화는 조질곡온에 관계없이 외기온도와 정백 후 곡온 차이로 발생한 결로에 의해 미세하게 증가하는 경향을 보였다. 정백 소비전력량은 조질곡온이 0℃보다 높은 조건이 2.52~14.28% 감소하는 것으로 나타났다. 따라서 동절기 정백시 현미의 적정 온도는 10℃ 이상으로 조질하거나 유지하는 것이 적절한 것으로 판단된다. This study was find out the relationships between whiteness, hardness, crack ratio, broken rice ratio, increased grain temperature, moisture content, electric energy consumption, and optimum conditioning of grain temperature for milled rice efficiency and quality maintenance during the milling process of brown rice at low temperature. There was no significant difference between the whiteness variations of white rice after milling process when the conditioning grain temperatures ranging from 0 to 20℃ was used. The crack and broken rice ratios of white rice and the hardness of brown rice decreased with an increased in the conditioning grain temperature. Conditioning grain temperatures of 12, 20℃ are applicable to appropriated standard of temperature (15℃) after milling process. In the case of low temperature of grain, there was steadily increase the rate of change moisture contents because of dew condensation. There was 2.62∼14.28% decline of electricity energy for milling process when temperature of grain was higher than 0℃. Therefore it needs to control temperature over 10℃ for appropriate milling in the winter season.

바나나 과실 함유탄닌이 소화효소 작용에 미치는 영향

정정한,류충호,조영수 東亞大學校附設遺傳工學硏究所 1997 遺傳工學硏究 Vol.- No.4

바나나 과실을 시중에서 구입하여 미숙과실과 황숙과실로 구별하고 이것을 과육부분과 과피부분으로 나누어서 동결건조 및 열풍건조시켜 실험 시료로서 사용하였다. 탄닌 함량을 분석한 결과 황숙과실 보다도 미숙과실에서 높았으며, 과육보다는 과피에서 열풍건조 보다는 동결건조시킨 시료에서 높은 경향을 나타내었다. 탄닌의 분획은 Sephadex LH-20칼럼을 사용하여 분획하였다. 바나나 과실에서 함유하고 있는 탄닌은 prodelphinidin, procyanidin을 구성단위로 하는 proanthocyanidin류의 축합성 탄닌으로 이러한 탄닌은 소화효소인 trypsin(EC 3. 1. 1. 3), α-amylase(EC 3. 4. 21. 4), lipase(EC 3. 2. 1. 1)에 대하여 in vitro에 있어서 저해작용을 가지고 있는 것이 확인되었으며 또한 탄닌은 중합도가 높을수록 저해율이 높다는 것이 확인되었다. It has been shown that tannins have adverse effects on growth of animals and feed utilization. Tannins are usually classified into hydrolyzable and condensed types but the adverse effects are more marked in condensed tannin in hydrolyzable tannin. Furthermore, the principle condensed tannins found in banana fruits are pro types by the polymerization of flavan-3, 4-diols either alone or in combination with other flavonoids such as catechins. Tannin of the investigated banana(Banana;Musa sapientum LINN)fruits was fractionated into four or five molecular forms, according to the degree of polymerization by chromatography on a column of Sephadex LH-20. The protein-precitating capacity of the fraction noted tannins increased in degree polymerzation. The inhibitory effect of tannins on trypsin(EC 3. 4. 21. 4), α-amylase(EC 3. 2. 1. 1) and lipase(EC 3. 1. 1. 3) activities in vitro also increased with the increased in degree of polymerization.

Miniplate를 이용한 악안면골 골절 환자의 Rigid Fixation 증례

한용환,박주현,정호균 중앙대부설한국의과학연구소 1989 한국의과학 Vol.21 No.3

都市地域住民의 運動實態와 關聯要因

南喆鉉,韓重鎬 韓國保健敎育學會 1991 보건교육건강증진학회지 Vol.8 No.1

This study was carried out to investigate consciousness level of physical exercise and analyze factors affection to the practice of exercise. The main purpose of the study was to give basic and necessary data in developing of program regarding to the physical exercise of the people and social athletics. This study was conducted by trained surveyers, for dweller in three cities(Seoul, Taegu and Ulsan) during January 4. 1990 to January 24. 1990. The results of this study can be summarized as follows 1. The subject of criticism an investigation for general characteristic be conducted in Seoul, Taegu and Ulsan city area an objective 417 person, 432 person and 366 person was among those comparatively little more by male was higher rate than female. Those in classification was adopted by age group 20 years old adult 4l.3% of most higher rate, age group evaluation, were 24.9% and other group were 18.4% comprehensively appearanced by adult group has most many value rate. 2. An objective of investigation survey was made to personnel were comparative an educated level significantly higher such as college graduated 48.8% this rate of value has most higher, High school graduated ; 30.1% and middle School gratuated were 11.7% . In addition the native comes from large city, Farming and fishing villages and rural area rate were shown as 29.6% ,28.4% and 19.9% each other. There by classfication of occupational job was shown by students has 27.4% are most higher significantly, also there sales and service field job appearanced 15.1% and expert technical job is 9.0%. Although nonreligion person rate were 37.3%, buddist, Christianity, Roman Catholicism all them each other shown 33.6%, 16.7% and 12.4%. An evaluation in economic situation value rate was appearanced by middle class level 61.7% and higher and low level are 14.4% and 23.9% with each other and married were 59.2% . 3. The people resident in cities area has pratice of health development by exercise person were 43.5% and value of rata for male has exercise practice is 52.5%, was significantly higher than female has by age group 10 years old and age group 50 years old is 52.6% and 47.3% was comparative higher rate also age group 30 is 35.1% of low factor was indicated. Although evaluated an economic situation rate was higher level get more taken the practice of health develop exercise(higher level 60.0% and low level is 32.9% ). Although by higher level of educated were more taken practice of their proper physical exercise(college graduated rate ; 52.2% of most higher and high school graduted : 39.7% and then middle school graduated is 19.1%) unmarried taken exercise rate : 48.4% is significantly higher than married person rate ; 40.8%. Although objective of native area of exercise rate of Urban area cities : 52.5% are significantly higher farming and fishing native person rate : 40.4% shown is most low. 4. The reason of not desired to applicant to physical exercise was reflect their owing to unavoidable circumstances rate 46.4% is significant higher rate, also not desired to have rate was 37.1%, not proper to take interest rate is 9.3%, applicant not desired to have exercise concerned to health program situation rate : 3.8%, Nevertheless health is weakness unable to take exercise rate ; 3.4% with each other. The reason of unable to taken exercise on comparatively appearanced by female has more higher than male, Although, when not desired to have person for unavoidable circumstances was appearanced female has more higher rate than male. This implication has shown age group 10 to 60 years old group has significantly higher than compare with other age group. Thereby classification of education level has been evaluated for its rate "by the not to desired" middle school graduated; 43.2 personnel owing to unavoidable circumstance rate of college graduated ; 43.9% is significantly higher. The classification of occupation by job be evaluated "personnel" circumstance in administrative job ; 69.2% and product job of rate : 66.7% , expert technic job of rate ; 65.0% was shown is significantly higher "not desired to" take exercise rate for students and unemployed were 47.4% and 36.4% of higher value rate. 5. The resident of people desired to have exercise required of the answered ; 49.4%, have an interest rate ; 24.7%, when get to opportunity is 4.3% and recommand by medical health member was adopted 0.9% is shown most low. The classification by resident of area to conduct the physical exercise rate of middle and small to town is 68.1% and 57.8% and resident large cities were having physical exercise rate ; 30.3% compare with them shown twice more higher rate appeared. However that the conduct of exercise with their interest by habit value appearances with resident in large cities has 31.3% significant higher compare with middle and small town person has practice rate: 20.8% and 18.8% rate of factors The objective of conduct the health development by sex was interested the goal of health improvement and their owing habitually practice sports were significantly higher by male than female. Although recommanded by families or medical health development members of female case has more higher than male. Nevertheless by age group be appearanced old person has significantly higher than young group age. Although there age 40 to 50 years old people were more higher than age 20 to 30 years old group. To evaluated the higher educated were more taken physical exercise by interest of habit but lower educated was most taken for goal of improve health situation by proper exercise make an effort. The classification of job performance respondent to practice the purpose of develop health with the practice of exercise when school teacher case; 81.8% was most significantly higher and having to take habit of interest were administrative job person has most higher rate. 6. The weekly taken practice of exercise person rate : 26.7% also twice taken rate is 15.7% but daily practice people are 17.1% by the male taken exercise once in a week has significantly higher but daily taken rate is female has more higher than male value of rate. The practice of weekly exercise rate was comprehensively higher avoid of age group 30 years old. Compare to age 30 years old has 37.0% is significantly higher by educated level be evaluated rate for daily taken exercise value has significantly higher. Although by classification of job was product job performance has most higher rate but practice of weekly taken exercise to be evaluated by labor job and administrative working group level has higher rate was indicated. 7. During weekly taken once exercise to practice an hour rate. 59.1% significantly higher and than halo hours taken rate is 2l.2% each other, and 30 minute taken rate: 7.2% also 3 hours taken rate ; 5.3. When male case taken an hour is significantly higher than female, but to three hours taken practice of exercise were shown female has more higher rate, younger age group has don't taken are more to increased of physic exercise, by the classification of job conduct of exercise taken an hour case appearanced significantly higher by product job and exper technical job performance has more higher rate, and then two hours taken exercise case were labor and unemployed level has significant higher rate be appearanced. 8. Although after taken drinking of exercise rate is about 40%, by the frequently taken or should be taken case are 7.3%, among by male is 52.8% be taken drinking after excecise and female was about 20% be takers the drinking. The comprehensive evaluation of after drinking by exercise by age group 50 to 60 were low rate to taken practice of exercise. However that have to be drinking were by age 20 to 30 years old got more higher rate than age of 40 to 50 years older. By the classification of educational level to evaluated be taken exercise for drinking rate by higher educated level and by classification of job in the drinking habit are after the exercise in product people job has shown 75.0% significantly higher compare with other group of age. When administrative job performance people has after drinking habit rate : 61.9% for secondly higher level be appearanced. To consideration by area in resident in larger cities for taken drinking for after the practiced the exercise were higher than compare to middle and small to town dweller. 9. To conduct in order to improve health maintain practice by conduct of exercise with sex β= .2011 of most affect higher influence level of education was BETA= .1288, By job EETA= .0578 and age group shows BETA = .0728. Those valuable rate about one percent level needed keep out attention (P<0.01). Their explanation capacity rate is 18.9% be point out 10. To discussed mention as above instruction was in order to maintain health development by practice of physical exercise dweller in cities capacity has compratively short rate compare to over all in the evaluations. Although, in the most larger city dweller has by age group 30 years old and middle to low level and female has practice of low in the conduct of physical exercise, nevertheless habit of after exercise to taken drinking habit shows in dweller in larger city people for age group was adult they got higher educated level person has job performance in the product field and male has significant higher rate was indicated. Therefore required gives constructive sprit of sports to having by their proper exercise be taken consciousness and prevent of illness for adult campaign required practice of proper physical exercise conduct is needed for the people of community life develop the health by sports to be proper taken action of an effort campaign is necessary under the conduct of development for physical health control by department of health and social affairs to cooperation mutual activities including department of government sports association committee and respectively medical health member assistance, committee of sports association members and all school been organized board of development health and education committee involve expert member for health development education conduct by effort of campain with under the political comprehensive performance by proper action be taken in order to proper program establishment for the dweller be attend increase to practice of physical exercise and sports conduct by the program be established of prevent illness for the adult effective in health maintain campain to achive community life to be make livelihood under the mutual cooperation and then establishment of proper political measurement program in order to dweller practice most handiness to exercise availabled to require facilities of installation for functional program develop and renovation activities campain is necessary.

하악지 사선골절단술에 의한 하악과두경부골절 치료 3예

정호균,한용환 중앙대부설한국의과학연구소 1988 한국의과학 Vol.20 No.3

Complex Composite 치아종 1예

한용환,정호균 중앙대부설한국의과학연구소 1988 한국의과학 Vol.20 No.4

위 전절제술을 받았던 환자에서 아급성 연합성 변성 1예

한정호,하현욱,이정석,송은향,김우정,김두응,최진영 건국대학교 의과학연구소 2000 건국의과학학술지 Vol.10 No.-

Subacute combined degeneration is a metabolic disorder of the spinal cord caused by vitamin B12 deficiency. A 72-year old man was admitted with weakness and tingling sensation of the bosh upper extremities. 9 years ago, he was diagnosed as having stomach cancer. So total gastrectomy with splenectomy was done at that time. The complete blood cell counts and morphology revealed megaloblastic anemia. Nerve conduction velocity study(NCV), electoromyography(EMG) and somatosensory evoked potentials(SEP) showed diffuse sensorimotor peripheral neuropathy. Magnetic resonance imaging(MRI) showed an increased T2-weighted signal, mild decreased T1-weighted signal, and slight contrast enhancement of the posterior columns of the cord in the cervical and upper thoracic spinal region. He was treated with vitamin B12 supplements and improved gradually. If abnormal sensation and muscle weakness was developed after gastrointestinal surgery, subacute combined degeneration should be considered in the differential diagnosis. We describe MRI findings of a typical case of subacute combined degeneration, which was induced by total gastrectomy.