Localization of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal Antibody

Kang-seuk Choi,Jin-ju Nah,Young-joon Ko,Shien-young Kang,Yi-seol Joo 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal AntibodyKang-seuk Choi*, Jin-ju Nah, Young-joon Ko, Shien-young Kang1 and Yi-seok JooNational Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea1Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, 48 Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk 361-763, KoreaReceived April 2, 2003 / Accept July 10, 2003J. Vet. Sci. (2003), 4(2), 167-173JOURNAL OFVeterinaryScience*Corresponding author: Kang-seuk Choi National Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea Tel: +82-31-467-1860, Fax: +82-31-449-5882 E-mail: choiks@nvrqs.go.kr

단일 처리기간에 의한 들깨의 생육 및 개화조절

최영환,최영훈,강점순,손병구,김용철,최인수,주우홍 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

It's not uncommon for the amount of leaves to be reduced significantly due to the early differentiation of flower-bud by short-day condition when growing perilla for harvesting the leaves in the greenhouse during the winter. This study analyzed the effect of day-length on the growth and regulation of flowering, which are closely related with differentiation of flower and amount of leaves. Development of new leaf was inhibited by short-day treatment for more than 5 days. As the period of short-day was longer, the effect on the development of new leaf inhibition was more evident. Number of leaves was reduced in both 40 days and 110 days short-day treatments as period of short-day length was longer. Short-day treatment for less than 3 days did not induce flowering, but short-day treatment for more than 5 days induced flowering. As the period of short-day treatment was shorter, percentage of flowering was lower and days to flowering was longer. That is, when treated for 20 days, flowering began in 20 days. Seed weight was reduced as the length of short-day treatment was longer.

상아질에 적용된 재 습윤제가 미세인장 결합강도에 미치는 영향

강희영,조영곤,김종욱,박병철,유상훈,진철희,최희영,기영재 大韓齒科保存學會 2004 Restorative Dentistry & Endodontics Vol.29 No.2

This study investigated that the effect of rewetting agent on dentinal microtensile bond strength (μTBS). Human molars were sectioned to expose the superficial dentin surfaces. Samples were divided into two groups according to type of adhesives-Single Bond (S) and One-Step (0)], and again subdivided into five groups by different dentin surface treatment-dry for 15s (D) , blot dry (BD) or dry for 15s, and rewet with different rewetting agents[distil1ed water (DW), Gluma Desensitizer (GD) and Aqua-Prep (AP)]for 30s. After application of adhesive, composite resin was built up on the bonding surface. Each tooth was sectioned to obtain stick with 1mm^(2) cross sectional area and the μTBS was determined by EZ test. In the S group, the mean μTBS of GD, AP and BD group was significantly higher than that of DW and D group (p < 0.05). In the 0 group, the mean μTBS of AP, GD, BD and DW group was significantly higher than that of D group (p < 0.05). The data suggested that Gluma Desensitizer and Aqua-Prep could be successfully used as rewetting agents, and Distilled water could be acceptable in aceton based adhesive system only.

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

암처리 엽수와 기간이 들깨의 생육 및 개화에 미치는 영향

최영훈,최영환,이영근,김용균,손병구,강점순,김근기 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

The lower leaves shading in upper leaves is unknown to be reduced significantly due to the early differentiation of flower-bud by dark treatment of leaves when growing perilla for harvesting the leaves in the greenhouse during the winter. This study analyzed the effect of dark treatment of leaves on the growth and regulation of flowering, which are closely related with differentiation of flower and amount of leaves. One leaf and 3 leaves per plant were dark treated for 0, 5, 10 days. Plant hight was reduced in 3 leaves treatment, but there was no difference in 1 leaf treatment. Stem diameter was reduced in both 1 leaf and 3 leaves treatments, which was more significant in 3 leaves treatment. Leaf area was reduced when 1 leaf was treated for 10 days, but there was no difference when 1 leaf was treated for 5 days. When 3 leaves were treated, leaf area was no difference between treatments. However, leaf area which 3 leaves were treated was reduced more significantly than that 1 leaf was treated. Seed weight was remarkably decreased when 1 leaf was treated for 10 days, and it was decreased when 3 leaves were treated for more than 5 days.

기내배양시 GA₃처리에 의한 들깨의 생육 및 개화조절

최영환,최영훈,강점순,손병구,이영근,주우홍 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

This study was conducted to estimate growth of leaf and shoot and flowering as affected by GA3 treatment in vitro. Plant height was longer as the concentration of GA3 was higher up to 2.0mg/l. Stem diameter was thick up to the concentration of GA3 was 0.1mg/l , but become thinner as the concentration of GA3 was higher. Root weight and root length were well developed in GA3 concentration was 0.5∼2.0mg/l. Number of roots was more in GA3 concentration was 0.5 and 1.0mg/l . The first flowering occurred in 40 days after 0.5 mg/l GA3 treatment. As the concentration of GA3 was higher, flowering was delayed. Number of flowering was significantly increased in GA3 concentration was 0.1 and 0.5mg/l. However, it was gradually decreased after 60 days. Percentage of flowering per plant was highest in GA3 concentration was 0.5mg/l , but it was lower as the concentration of GA3 was increased.

One step bulk modification of poly(L-lactic acid) composites with functional additives to improve mechanical and biological properties for cardiovascular implant applications

Kang, Eun Young,Choi, Bogyu,Park, Wooram,Kim, Ik Hwan,Han, Dong Keun Elsevier 2019 Colloids and Surfaces B Vol.179 No.-

<P><B>Abstract</B></P> <P>Poly(L-lactic acid) (PLLA) has been widely used as a promising biomaterial in biomedical applications due to its biodegradability and high mechanical strength. However, because of the inherent brittleness, low impact resistance, and weak thermal stability of PLLA, the modification process is usually required to utilize it for biomedical devices. Furthermore, acidic byproducts resulting from the hydrolysis of PLLA after implantation reduce the pH of the surrounding environment and cause inflammatory responses in the implanted area, leading to the failure of their clinical applications. To this end, here, we demonstrate a novel modification process for the PLLA composite with various functional additives, such as cis-aconitic anhydride (AA), triacetin (TA), isosorbide derivative (ISB), and/or Pluronic® F127 (F). The modified PLLA composite with TA and F (PLLA/TF) showed significantly improved elongation at break and Young’s modulus and retained tensile strength. Moreover, incorporating magnesium hydroxide (MH) nanoparticles (PLLA/TFMH) significantly reduced acid-induced inflammation responses caused by the acidic degradation products of PLLA. Reduced plasma protein adsorption was observed in the PLLA/TFMH. These results suggest that the one step bulk modification of biodegradable PLLA using TA, F, and MH will have great potential in cardiovascular implant applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Improved mechanical properties of PLLA via one step bulk modification with TA and F. </LI> <LI> Reduced acid-induced inflammation via one step bulk modification of PLLA/TF with MH. </LI> <LI> Increased homogeneity of MH distribution in one step bulk modified PLLA/TFMH. </LI> <LI> Enhanced hemocompatibility of PLLA/TFMH. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

미치광이풀 모상근 배양에서 Tropane Alkaloid 생산성 증진을 위한 최적 생물학적 엘리시터 선발

정희영,강승미,강영민,김용덕,양재경,정영관,최명석 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Scopolamine and hyoscyamine which belong to tropane alkaloids are the pharmaceutically valuable anticholinergic drugs. In order to increase the productivities, the effects of elicitation were investigated during hairy root cultures of Scopolia parviflora. Biotic elicitors originated from 3 fungi and 1 yeast were prepared as homogenate and supernatant and added to 3-week-old cultures. Both of homogenate and supernatant of Candida albicans elicitors increased the scopolamine production. The production of hyoscyamine was enhanced by homogenate of Fusarium solani and supematant of C. albicans. Most of the other fungal elicitors were also improved on the tropane alkaloid production compared to non-treatment. Among the elicitors tested, C. albicans was proved the optimal biotic elicitor on tropane alkaloids production. These results will be served mass production of tropane alkaloids by large-scale production.

시청률 제고를 위한 KBS의 2014 월드컵 중계방송 전략에 대한 연구

최준서(Choi, Joon-Seo),배재성(Bae, Jay-Song),강영희(Kang, Young-Hee) 한국체육과학회 2015 한국체육과학회지 Vol.24 No.6

The purpose of this study is to analyse the strategy of KBS World Cup Broadcasting in 2014 pertaining to its commentator and sportscaster in leading its broadcasting to the best program with the highest audience ratings. It is also assessed as to how KBS World Cup commentator and sportscaster was selected and why they could have a magnetism to successfully attract the TV viewers In the run up to the 2014 World Cup, KBS have employed a new commentator with expertise and experience as former South Korean football international and choose a relatively young sportscaster who could fit in well with the commentator. This strategy carried forward by KBS struck a chord with the TV viewers: Young-Pyo Lee, the KBS commentator was praised for his expertise and ability to predict the outcomes of important World Cup matches, a style that was in stark contrast with more frivolous and entertaining broadcasting of MBC. Also KBS could put SBS with the established commentator into the shade by virtue of Lee’s exceptional capabilities in analysing World Cup games. It is safe to say that the success of KBS World Cup broadcasting greatly relied on the ability of its commentator as a football analyst. It is a reassurance that the expertise of commentator is the most important factors in dictating ratings of the World Cup broadcasting.

Anti-osteoporotic Effects of Sericin-Calcium Conjugate in Ovariectomized Rats

Ehn-Kyoung Choi, Dongsun Park, So-Young Choi , Yun-Hui Yang, Seock-Yeon Hwang, Seongho Yeon, Jeong-Yong Lee, Ill-Hwa Kim, Yun-Bae Kim, Hyun-Gu Kang1 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.2

This study was conducted in order to evaluate the effect of sericin-calcium (SC) as therapy for ovariectomy-induced osteoporosis in rats. Three weeks after ovariectomy (OVX), Sprague-Dawley rats were divided randomly into three groups: sham-operated group (Sham), ovariectomized group, and SC-treatment group (OVX+SC). Rats in the OVX+SC group were given drinking water containing 0.07% SC for eight weeks. Bone breaking force, mineralization, and blood parameters related to bone metabolism were analyzed. In OVX animals, blood concentration of 17β-estradiol showed a significant decrease, while osteocalcin and type I collagen C-terminal telopeptides (CTx) showed an increase. Breaking force of femurs as well as bone mineral density (BMD), ash, calcium, and phosphorus in femurs showed a significant decrease following OVX. Treatment with SC (0.07% in drinking water) resulted not only in remarkable restoration of the decreased 17β-estradiol and increased osteocalcin and CTx concentrations, but also led to recovery of decreased femoral breaking force, BMD, ash, calcium, and phosphorus. It is suggested that SC effectively improves bone density by preventing bone turnover-mediated osteocalcin, CTx, and minerals, and that it could be a potential candidate for use in therapy or prevention of post-menopausal osteoporosis.