한국인 알코올 의존 환자에서 알코올 대사 효소 유전자형 빈도의 남녀 차이

김성곤,김철민,이덕기,황인복,이현숙,김성연,전은숙,송영상,박제민,최병무,김명정 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.2

Objectives : There are a number of preceding epidemiological studies reporting gender differences in the genetic etiology of alcohol dependence. The author investigated gender difference in the frequencies of ADH2 and ALDH2 genoypes between the patients with alcohol dependence and normal control. Methods : The subjects were 141 alcohol dependent patients (104 males, 37 females) and 138 normal control (79 males, 59 females). The frequencies of 1/1 and 1/2+2/2 (2+ afterward) genotypes for ADH2 and ALDH2 were investigated in male and female between alcohol dependence and normal control group. DNA was extracted from WBC in peripheral venous blood and PCR-RFLP method was used out for genotyping. Results : First, the frequency of ADH2 1/1 genotype was significantly higher in alcohol dependent patients than normal control in both genders. Second, while there was no gender difference in the frequency of ADH2 1/1 genotype in normal controls, in the patient group however, the frequency was significantly higher in females than males. Third, in male subjects with alcohol dependence, the frequency of ALDH2 1/1 genotype was significantly higher than in male normal control subjects. On the other hand, in female subjects with alcohol dependence, the frequency of ALDH2 2+ genotype was significantly higher than in female normal control subjects. Conclusion : These results suggest that while the risk of alcohol dependence is predominantly affected by ALDH2 1/1 geno-type in male, the female ADH2 1/1 genotype is mainly associated with the risk of alcohol dependence. This means that there are gender differences in the genetic etiology of alcohol dependence.

전국 단위 감시체계의 현황과 전망

강성규,안연순,지영구,김형옥,하미나,권호장,백남종,김성아,홍윤철,김재용,강대희,정혜선,하은희 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.2

8년간(1992~1999) 산업안전보건연구원에 의뢰된 직업병 심의 사례 분석

강성규,김규상,김양호,최정근,안연순,진영우,최병순,양정선,김은아,채창호,최용휴,김대성,박정선,정호근 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : Pneumoconisis and noise-induced hearing loss (NIHL) have been reported as main occupational diseases by the Special Health Examination. The Industrial Accident Compensation Insurance has reported various work-related diseases, however, these two diseases occupied almost a half of compensated cases. Therefore, it was not well known about the status of occupational diseases other than pneumoconiosis, NIHL, and cardio-cerebrovascular accident (CVA). This study was conducted to analyze claimed cases as an occupational disease, that was requested to the Korea Industrial Safety and Health Agency (KOSHA). Methods : The local office of the Korea Labor Welfare Corporation (KLWC) has asked the KOSHA for confirmation of claimed cases as an occupational disease. We analyzed 379 cases requested from KLWC, the Ministry of Labor, employers, unions and occupational health agencies from 1992 to 1999. Results : Male was 80.7 % of the requested cases. Their mean age was 42 years old and 75.5 % of them were more than 35 years old. The requested cases were increased rapidly from 25 cases in 1992 to 108 cases in 1999 and the accept rate was 50.7 %. The majority of requested cases were respiratory diseases(22.4%), cancers(18.5%), Neuropsychiatric problems(14.5%), and musculoskeletal problems(13.5%). The accept rate was high in reproductive, respiratory, musculoskeletal and digestive disorders and low in neuropsychiatric, renal and otologic problems and occupational cancers. 73.6% of them were caused by chemical agents, especially 28.5 % were by organic solvents. 67% of them were clinically confirmed at university hospitals. A half of the cases were from KyongIn area, even the request came from the whole country. Conclusions : A claim was common in workers whose age was over 35 years old and exposure history was over 10 years. The respiratory diseases and neuropsychiatric disorders were still main problems in occupational health and occupational cancers was increasing even though its accept rate was not high yet.

플레이트거더의 전단강도

최취경,김태영,서성연,김정석,김상섭,김규석 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

In the design of plate girder web panels, it is required to evaluate accurately the elastic buckling strength under pure shear. Whether or not the post-buckling strength is accounted for. Currently. Elastic shear buckling coefficients of web panels stiffened by transverse intermediate stiffeners are determined by assuming conservatively that web panels are simply supported at the juncture between the flange and the web. Although the notion of the real boundary condition at the juncture of the web and flanges to be somewhere between simple and fixed has been recognized from early days, the boundary condition has been conservatively assumed. Mainly due to lack of means to evaluate it in a rational manner. In this paper. A series of numerical analyses based on a three-dimensional finite element modeling is carried out to investigate the effects of the geometric parameters on the boundary conditions at the juncture between the flange and the web.

24주간 추적하는 임상 연구에서 한국인 남자 알코올 의존 환자의 비재발률

강철중,김성곤,남궁기,조동환,이병욱,최인근,최용성,박성봉,제영묘,김현경,김성연 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.1

Objetives : In order to provide useful data tbr the further clinical studies with Korean alcohol-dependent patients, enrollment and non-relapse rates were investigated while alcohol-dependent patients wcre followed up for 24 weeks. Methods : The subjects of this study were alcohol-dependent male patients who, for the first time, visited a hospital among 3 groups of hospitals, University Hospital, General Hospital or Mental Hospital, or those who needed to be followed up after discharge from those hospitals. After assigning 12 subjects to each hospital, we investigated enrollment and non-relapse rates while cognitive-behavioral therapy and pharmacotherapy were provided. Results : 1) Forty-eight patients were enrolled and the rate of enrollment was 36.4%. The enrollment rate in the University Hospital group was 52.1%, in the General Hospital group 50.0%. and in the Mental Hospital group 10.4%. 2) In 48 patients, non-relapse rate was 27.1% at 12 week and 18.8% at 24 weeks after starting follow-up. It was 24.4% and 20.0% at 12 and 24 weeks, repectively, in the University Hospital group while the General Hospital group showed 33.3% and 16.7%, respectively, and the Mental Hospital Group showed 20.0% at both 12 and 24 weeks. Conclusion : These results provide important data for further clinical studies of Korean alcohol-dependent patients.

방사선조사가 금식 흰쥐 위에 미치는 미세구조의 변화

김부순,성연문 대구보건대학 1998 대구보건대학 論文集 Vol.18 No.-

In this study, we examined the ultrastructural influence of radiation on rats stomach tissue, the difference between NPO and con in the rats stomach was measured after a single irradiation to 15 Gy dose, the results follow ; Widening of intercellular space, and swelling and degeneration of secretory granules in chief cells were observed from NPO rats of a day after irradiation. In mucosal epithelial cells of con rats of 7 days after irradiation, destruction of microvilli was similar to from NPO rats of a day after irradiation but morphology was cuboidal. Parietal cell proliferation and differentiation were active as well as mucous epithelia structures were stable like to control rats from con of 14 days after irradiation. But NPO rats were not until 28 days after irradiation that mucous epithelial structures were stable too. On the basis of this results, we know obviously that NPO will be a factor of stomach abnomalities while hospital patients are treated with high-energy irradiation.

통계처리의 오용에 관한 연구

김성연 동아대학교 경영대학 1989 經營硏究 Vol.- No.3

When using statistical methods, we often make errors. So, their causes are investigated over the whole statistical processes. In this paper, the statistical processes are divided into three steps, i.e. i) sampling step, ⅱ) theory-application step, iii) result - interpretation step. And in each step, we consider the statistical misunderstandings, errors and the method by which we can correct them.

다꾸지법을 이용한 구리와 타이타늄 마찰압접부의 용접변수의 영향에 관한 연구

金成演,延倫模,鄭承富,徐昌濟 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.7

This paper describes a fundamental investigation of the friction welding condition for the pure copper/ pure titanium joining and the effect of friction time, upset pressure on the mechanical and metallurgical properties of the joint. Taguchi method has been adopted for investigating the contribution of welding variables. Under a constant upset pressure, the tensile strength have a little difference with an increase in friction time. At a constant friction time, the tensile strength increased with an increase in the upset pressure. The tensile fracture of Cu to Ti joint occurred at the Cu base material near the interface.

연속주조에서 Upper Nozzle 막힘지수에 의한 막힘 거동 고찰

김성연 대한금속재료학회 2002 대한금속·재료학회지 Vol.41 No.2

The characteristics of upper nozzle clogging was investigated using upper nozzle clogging index(CI_Ar) in a continuous casting of steel. CI_Ar was derived from the modified value of argon back pressure, which was dependent on the argon flow rate in upper nozzle, liquid steel throughput, and weight of liquid steel in a tundish. Inclusion attachment to and its dislodging from upper nozzle, the phenomena of which could be detected from the slow increase and the following sudden decrease of CI_Ar, was found to occur continuously for all sequences of cast in case of severe nozzle clogging.

대장균에서 재조합 Rat Guanine deaminase 유전자의 발현, 정제 및 분석

성연선,곽상준,박대성,김향원,이희영 단국대학교 1998 論文集 Vol.33 No.-

Guanine deaminase(EC, 3.5.4.3, ;Guanine aminohydrolase, GAH, GDA) catalyzes the deamination reaction of guanine to xanthine irreversibly. The cDNA encoding rat guanine deaminase had been isolated from a λZAPII rat brain expression library using antibody generated against purified rat guanine deaminase. Toe obtain recombinant GDA and analyze the property of catalytically conserved retion, here we expressed the recombinant GDA in E.coli and showed the retention of its catalytic activity similar to native rat GDA. To make the construct carrying coding region to GDA in pGEX4T prokaryotic expression vector, the region encompassing open reading frame of pBlue-GDA was PCR amplified and subcloned into pGEX4T prokaryotic expression vector with correct reading frame of fusion carrier glutathione S-transferase. After transformation to E.coli DH5a. The bacteria carrying pGEX-GDA was grwon in the condition of IPTG induction. The fusion protein GST-GDA was purified with GSH-sepharose affinity chromatography. The purified GST-GDA was subsequently digested with biotin-conjugated thrombin, and thrombin was removed by streptavidin agarose. Then sample were treated with GSH-sepharose affinity chromatography to remove the contaminant GST and GST-GDA. The purified recombinant GDA showed 70% of specific activity relative to that of purified rat GDA. The recombinant GDA showed identical molecular weight with rat GDA in SDS-PAGE.