Purge & Trap-GC를 이용한 의약품 필름코팅 정제 중 잔류용제에 관한 연구

장준식,이명자,소유섭,문춘선,이주헌,박희라,김진숙,강경모,이선옥,방성연,유미자,유문균,금오성,이병욱 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

의약품은 약물을 생체에 적풋하기 위하여 유효성분의 효과가 언제나 일정하게 확보되고 사응에 편리하도록 만들어지는 것이므로 유효썽분 이외에 약효에 영향을 주지 않는 성분이 첨가되는 경운가 많다. 이 때 사용되는 용매들은 제피의 광택 및 건쪼시간의 단축 등을 위하여 휘발점이 낮을 용매들이 주로 사용되어진다. 본 연구는 의약품 필름코팅정제 중 잔류용매 4종(chlorofonr benzen, trichloro ethylen, 1,4-dioxane)에 대한 변형된 pirge & trap-GC 장치를 이용한 동시분석방법을 개발하였으며, 각 표준품의 RSD 값은 chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69% and 1,4-dioxane 3.41%였다. 또한 시중 유통중인 의약품 50종에 대하여 잔류웅매 양을 측정하였으며, 검출되는 잔류용매는 한 건도 없었다. This study nras carried out to develope the analytical method for the mixture of chlorefonn, benzen, trichloroethylen and 1,4-dioxane simultaneously and determine the remainingorgauic solvents in coating tablets by Purge & Trap-GC. The results were as follouFs ; 1. Chloroform, benzen, trio:tloroethylen and 1,4-dioxane separated by tenax #5 trap by HP-624GC column by terrlperature programming. The peaks were separated completely at retentiontime of 6.88min for chloroform, 8.21min for benzen, 10.38miu for trichloroethylen and 11.95minfor 1,4-dioxane. 2. Standard RSD were individually chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69%and 1,4-diorane 3.41%. 3. 60 samples were not detrcted chloroform, benzen, trichloroethylen and 1,4-dioxane.

Candesartan과 Lercanidipine의 병용투여에 의한 혈압 하강 상승효과

한주희,이정진,김요한,명창선 충남대학교 약학대학 의약품개발연구소 2011 藥學論文集 Vol.26 No.-

Immunoblot법을 이용한 Anisakis증의 혈청학적 진단

이영주,주경환,정명숙,임한종 고려대학교 의과대학 1990 고려대 의대 잡지 Vol.27 No.1

Acute gastric anisakiasis is rather easily diagnosed by endoscopy or radiological technique. But preoperational diagnosis of acute intestinal anisakiasis is very difficult. Furthermore, the diagnosis of chronic anisakiasis is more difficult because the worm invade the stomach or intestinal wall and finally to degenerate. Serodignosis may helpful in such diagnostic difficulties. However, any of the serodiagnostic method in anisakiasis has limitations for practical use due to cross reactions. In this context, the study was performed to observe the specifically reacting antigenic bands of crude extract Anisakis larval worm antigen and their reaction to the serum antibody obtained from experimentally immunized and infected rabbit, confirmed human anisakiasis and other helminthic infection cases using SDS-PAGE and EITB. The results obtained were as follows; 1. Among 12 cases of confirmed anisakiasis, eleven showed positive reactions by ELISA. The average 0. D. value of the test was 1.035. Two from 4 D. latum infected cases and one from 4 A. lumbricoides infected cases showed positive reaction respectively. 2. Silver stained SDS-PAGE of crude Anisakis larval worm antigen revealed more than 45 antigenic protein bands. Among them, 186, 76, 72, 65, 59~62, 54, 52, 50, 49, 46, 43, 40, 33, 32, 29, 24, 23, 18, 17, and 13Kd bands were major bands. Protein bands of 12Kd relative molecular weight was observed in A. suum extract antigen, on the other hand 29 and 46Kd bands were not observed. 3. Major antigenic bands of crude Anisakis worm antigen which reacted with serum antibody of confirmed anisakiasis cases were 186, 116, 72, 52, 46, 40, 33 and 32Kd band. Most of them also reacted with the sera of D. latum, A. lumbricoides, T. trichiura infected cases except 46Kd band which showed positive resuits only with sera of anisakiasis. So, this band could be considered as diagnostic band for anisakiasis especially for the identification of cross reactions against A. lumbricoides and D. lafum infection. 4. Although, low molecular weight bands such as 33 and 32Kd were observed as species specific, the sensitivity of that fractions were obviously low. Consequently, the diagnostic use of the low molecular weight protein bands were not promising. 5. Serum of rabbit immunized with Anisakis larval worm antigen also showed positive reaction on 46Kd bands, but that of A. suum and T. canis did not. These results were in accord with the results in human cases. In conclusion, 46Kd bands of crude Anisakis larval worm antigen can be considered as a diagnostic one for the identification of cross reactions using immunoblot technique.

폴리머타임을 이용한 슈퍼그래픽 개발

이명호,이주희,이강우 강원대학교 석재복합신소재제품연구센터 1998 석재연 논문집 Vol.3 No.-

폴리프로필렌을 이용한 경량바닥재 개발

이윤수,주명기 강원대학교 부설 석재복합신소재 제품연구센터 2003 석재연 논문집 Vol.8 No.-

본 연구에서는 강도 및 내구성이 우수한 폴리프로필렌을 이용한 경량바닥재를 개발할 목적으로 중질탄산칼슘 및 폴리머를 결합재로 하고 목분을 골재로 한 폴리머 모르타르를 제조하여 폴리머 모르타르의 흡수율, 휨 및 압축강도에 미치는 목분 혼입량의 영향에 대하여 검토하였다. 그 결과로, 폴리머 모르타르의 흡수율은 목분혼입량의 증가에 따라 증가하는 경향을 보였다. 또한 폴리머 모르타르의 휨 및 압축강도는 목분 혼입량의 증가에 따라 감소하는 경향을 나타냈다. In this study the lightweight polymer composites were made in which the polypropylene as binder, the heavy calcium carbonate as filler, the splinter as aggregate. The effects of splinter content on the water absorption, flexural and compressive strengths of lightweight polymer mortars with polypropylene and splinter are examined. As a result, the water absorption of the lightweight polymer mortars with polypropylene tend to increase with increasing splinter content. The flexural and compressive strengths of the lightweight polymer mortars with polypropylene tend to decrease with increasing splinter content.

영유아예방접종시 보건소이용가정과 병의원 이용가정의 사회계층적 특성에 관한 분석연구

李恩珠,李明淑,車喆換 고려대학교 의과대학 1991 고려대 의대 잡지 Vol.28 No.3

In order to develop an appropriate health care service program for a population. it is of utmost importance that the particular population's demograhic and socio-economic characteristics are taken into consideration. The purpose of this research was to determine which of the demographic and socio-economic factors gathered from basic family health records contribute to the decision by people to use either the hospitals and clinics. or health centers for their children's health care. The target area of this study was Guro 6 dong. Guro-Ku, Seoul. A sampling method of choosing families whose children(1-3 years old) had already received their third DPT vaccination resulted in 405 subject familes out of 3904 family health records which were on file at the Guro 6 dong health subcenter A general survey of the households under study revealed that 61.9% of the children received the vaccinations at a health center. and 38.1%. at a clinic or a hospital. The demographic. socio-economic. and maternal health behaviour characteristics were all analyzed for each of the 405 families. In addition. the likelihood of each family to use either the clinic and hospital or the health center was analyzed. Significant differences in the data were determined by the Chi-squared methcd. The factors affecting the utilization of medical facilities ware examined through a stepwise discriminant analysis. using the type of medical facility utilized as group variable, and the demographic characterlistics socio-economic characteristics. or maternal health behaviour as discriminant variable. 1. Of the demographic characteristics. father's education. the mother's age. mothor'education. and the mother's occupation had a hit ratio of 58.0% 2. Upon the analysis of socio-economic characteristics, type of housing, status of ownership of the house, household income, and whether the family had a telephone had a hit ratio of 69.9%. 3. Using maternal health behaviour. as the discriminating variable, and prenatal care, postpartum care had a hit ratio of 70.9%. 4. The stepwise discriminant analysis resulted in postpartum care, type of housing, ownership status of the house, mother's occupation, monthly income, and ownership of telephone having a hit ratio of 73.4%. The collection of basic health information of a population is essential for planning an appropriate health care service program. This research has shown that, as part of the basic information, demographic, and scoio-economic, as well as maternal health behavior characterisics are very important in determinizing an individual's preference for a particular type of medical facility from the available ones. Furthermore, these specific characteristics of a population may be used in planning a comprehensive health care service program to make certain appropriate facilities and programs are available. More need to be done in the future to discover the factors which influence the effective utilization of health service programs available to the population.

현훈 환자 Neuron Specific Enolase의 임상적 의의

김원주,김선곤,유철형,김원찬,김용덕,최영철,이명식 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

인공타액에서 수종 아말감의 부식시 용해성분 및 표면 부식 생성물에 관한 실험적 연구

이명종,조승주 大韓齒科保存學會 1994 Restorative Dentistry & Endodontics Vol.19 No.1

GC와 GC-MS에 의한 어란의 지방산 분석

이주현,전명숙,이명환 서울여자대학교 자연과학연구소 1995 자연과학연구논문집 Vol.6 No.-

9종류의 어란에서 지방산을 추출하여 GC-MS로 분석한 결과 다음과 같은 결과를 얻었다. 1. 어란중 지방산을 GC와 GC-MS로 분석할 때 초기온도를 60°C로 하고 승온률을 9°C/min, 마지막 온도를 220°C로 하는 승온법이 목적 성분을 분리하는데 가장 좋은 결과를 얻었다. 2. 가자미, 날치, 대구, 명란, 농어, 성어, 오징어, 참치, 청어의 알에서 지방산을 추출하여 GC로 분석한 결과 포화 지방산은 myristic acid, palmitic acid, stearic acid, arachidic acid가 검출 되었고, 불포화 지방산은 oleic acid, linolenic acid 가 각각 확인 되었다. 3. 포화 지방산은 myristic acid가 명태알에 11.8㎍/g 으로 가장 많이 함유되어 있었고, palmitic acid는 오징어알에서 106.32㎍/g으로 가장 많이 함유되어 있었다. Stearic acid는 농어알에 53.03㎍/g, arachidic acid는 대구알에 8.5㎍/g으로 가장 많은 양이 함유되어 있었다. 4. 불포화 지방산인 oleic acid와 linolenic acid의 함량을 분석한 결과 oleic acid는 가자미 알에 linolenic acid는 명태알에 각각 109.12㎍/g, 62.48㎍/g으로 가장 많은 양이 함유되어 있었다. Fatty acids were extracted from the roe of flounder, flying fish, Pacific cod, Alaska Pollack, mero, shark, squid, tuna, and herring. They were methylated and analyzed by GC and GC-MS. Most of fatty acids found from 9 different roe were myristic acid, palmitic acid, stearic acid, arachidic acid, oleic acid, and linolenic acid. The roe of Alaska Pollack, squid, mero, and Pacific cod contained 11.8ug/g, 62.48 ug/g, of unsaturated fatty acids such as oleic acid and linolenic acid, respectively. The roe of flounder and shark had higher content unsaturated fatty acids than rest of the roe. The roe of Pacific cod, Alaska Pollack, mero, squid, tuna, and herring had larger amount of saturated fatty acids than the rest of the roe.

참외주스 가열농축에 따른 관능적 특성 변화

이기동,권승혁,이명희,김숙경,주길재,권중호 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2004 한국식품저장유통학회지 Vol.11 No.1

고품질 참외 농축액을 제조하기 위해 농축공정에서 중요한 변수로 작용하는 가열시간을 달리하면서 제조한 농축물에 대한 관능적 평가를 실시한 결과, 가열 후 맛이 최대평점을 나타낸 조건은 가열온도가 96.63 ℃ 및 가열시간이 14.31 min일 때 였으며, 전반적인 기호도가 가장 높은 조건은 가열온도가 97.18℃ 및 가열시간이 14.55 min 일 때 였다. 가열온도 및 가열시간을 달리하면서 실험한 결과 참외 농축액 제조를 위해서는 가열온도 98℃로, 가열시간 13 min으로 처리하는 것이 적당하였다. This study was carried out to observe the change of organoleptic properties of the juice prepared with various heating temperature and heating time. Organoleptic color showed maximum value at 94.95℃ and 21.63 min and organoleptic aroma showed maximum value at 63.14℃ and 20.38 min. Organoleptic taste showed maximum value at 96.63℃ and 14.31 min and overall palatability showed maximum value at 97.18℃ and 14.55 min The best condition was 98℃(heating temperature) and 13 min(heating time) at oragnoleptic evaluation of oriental melon juice.