서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

흰쥐 시상하부에서 NMDA에 의한 c-fos의 발현

이영기,최기열,이원돈,유영복,이왕재,황덕호,조사선,장가용 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

Tetrahydroisoquinoline Alkaloids가 쥐의 동맥과 뇌균등질의 α-Adrenoceptors에 미치는 영향

이현석,백선하,황수현,박인성,김은상,정진명,한종우,장기철,장기철 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.1

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

Focal adhesion kinase and src expression in premalignant and malignant skin lesions

( Ju Yeon Choi ),( Jae Yun Lim ),( Han Saem Kim ),( Jung Min ),( Jung In Kim ),( Hyun Min Seo ),( Sang Hyeon Hwang ),( Chong Won Choi ),( Yoon Hwan Kim ),( Jin Hee Sohn ),( Hyunjoo Lee ),( Won Serk Ki 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Focal adhesion kinase (FAK) and Src are non-receptor tyrosine kinases. FAK and Src play a critical role in inducing malignant transformation in tumor cells. Objectives: We performed immunohistochemical staining for total and phosphorylated forms of FAK and Src, to evaluate the role of FAK and Src in the development of premalignant and malignant skin lesions. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Results: Cells positive for t-Src, p-Src-y530, t-FAK and pFAK-s722 were detected in premalignant intra-epithelial lesions (PELs) and squamous cell carcinomas (SCCs), but not in the perilesional skin. There was a tendency towards high correlation between Ki-67 and t-FAK or pFAK-s722, suggestive of the active role of FAK in cell proliferation. Conclusion: Our findings of higher t-Src and p-Src-y530 positive cells in PELs, as compared to SCCs (with higher Ki-67 level), are suggestive of the other role of Src in tumor formation and progression, which requires further investigation.

Effects of Cu,Zn-superoxide dismutase on cell proliferation and neuroblast differentiation in the mouse dentate gyrus.

Yoo, Dae Young,Shin, Bich Na,Kim, In Hye,Kim, Woosuk,Kim, Dae Won,Yoo, Ki-Yeon,Choi, Jung Hoon,Lee, Choong Hyun,Yoon, Yeo Sung,Choi, Soo Young,Won, Moo-Ho,Hwang, In Koo Kluwer Academic/Plenum Publishers 2012 Neurochem Res Vol.37 No.2

<P>Oxidative stress is one of the most important factors in reducing adult hippocampal neurogenesis in the adult brain. In this study, we observed the effects of Cu,Zn-superoxide dismutase (SOD1) on lipid peroxidation, cell proliferation, and neuroblast differentiation in the mouse dentate gyrus using malondialdehyde (MDA), Ki67, and doublecortin (DCX), respectively. We constructed an expression vector, PEP-1, fused PEP-1 with SOD1, and generated PEP-1-SOD1 fusion protein. We administered PEP-1 and 100 or 500 관g PEP-1-SOD1 intraperitoneally once a day for 3 weeks and sacrificed at 30 min after the last administrations. PEP-1 administration did not change the MDA levels compared to those in the vehicle-treated group, while PEP-1-SOD1 treatment significantly reduced MDA levels compared to the vehicle-treated group. In the PEP-1-treated group, the number of Ki67-positive nuclei was similar to that in the vehicle-treated group. In the 100 관g PEP-1-SOD1-treated group, the number of Ki67-positive nuclei was slightly decreased; however, in the 500 관g PEP-1-SOD1-treated group, Ki67-positive nuclei were decreased to 78.5% of the vehicle-treated group. The number of DCX-positive neuroblasts in the PEP-1-treated group was similar to that in the vehicle-treated group. However, the arborization of DCX-positive neuroblasts was significantly decreased in both the 100 and 500 관g PEP-1-SOD1-treated groups compared to that in the vehicle-treated group. The number of DCX-positive neuroblasts with tertiary dendrites was markedly decreased in the 500 관g PEP-1-SOD1-treated group. These results suggest that a SOD1 supplement to healthy mice may not be necessary to modulate cell proliferation and neuroblast differentiation in the dentate gyrus.</P>

전기도금을 이용한 스테인레스 스틸 각형 선재의 굵기 증가 후 물성 변화

이정석,이기헌,황현식 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.2

본 연구는 전기도금을 이용하여 각형 선재의 굵기를 증가시킨 후 기존의 선재와 물성을 비교함으로서 이의 임상적 적용 가능성을 알아보고자 시행되었다. 한 변의 길이가 0.016 인치인 정사각형 스테인레스 스틸 교정용 선재에 니켈 전기도금을 시행하여 굵기를 0.01 인치 증가시켜 한 변의 길이가 0.017 인치인 정사각형 선재로 만들었고 도금층의 밀착성을 증진시키기 위해 400℃의 전기로 에서 10분간 열처리를 시행하였다. 시편의 물성변화를 알아보기 위하여 전기도금을 이용하여 제작된 15개의 시편을 실험군(016P군)으로, 기존의 0.016인치(016), 0.017 인치(017군) 스테인레스 스틸 교정용 선재를 대조군으로 설정한 후 3점 굴곡 시험과 비틀림 시험을 시행하여 다음과 같은 결과를 얻었다. 1. 3점 굴곡 시험 결과 016P군에 비해 강성과 항복강도, 국한강도 모두 증가하는 경향을 보였으며, 강성과 극한강도는 통계적으로 유의한 차이가 있었다(p<0.05). 2. 3점 굴곡 시험 결과016P군이 017보다 강성과 항복강도, 극한강도 모두 낮은 경향을 보였으며, 강성은 통계적으로 유의한 차이가 있었다(p<0.05). 3. 비틀림 시험 결과 016P군이 016군에 비해 비틀림 모멘트, 최대 비틀림 모멘트 모두 높았으며, 통계적으로 유의한 차이가 있었다(p<0.05). 4. 비틀림 시험 결과 016P군이 017군에 비해 비틀림 강성과 항복 비틀림 모멘트, 최대 비틀림 모멘트 모두 낮은 경향을 보였으며, 이 중 항복 비틀림 모멘트와 최대 비틀림 모멘트에서 통계적으로 유의한 차이가 있었다(p<0.05). 이상의 결과를 요약해 보면 각형 선재에 도금을 시행하여 굵기를 증가시키고 물성이 증가될 수 있음을 알았다. 비록 도금을 시행한 016P군이 기존의 017군보다 강성이 낮았으나 이는 반대로 016P군이 017군보다 탄성이 높음을 나타내며 토크조절이 부족한 경우 브라켓 슬롯에 쉽게 삽입되어 교정력을 발휘할 것으로 기대된다. The purpose of this study was to evaluate clinical applications of electroplating method through investigation of the physical properties of orthodontic rectangular wires according to varying their cross section. For the study, it was accomplished to electroplate the 0.016-inched orthodontic rectangular stainless steel wire. The cross section of stainless steel orthodontic rectangular wire increased from 0.016 x 0.016 inch to 0.017 x 0.017 inch by electroplating. The wire was heat treated to improve an adhesion between the wire and electroplated metal. A three=point bending test and torsion test were conducted in order to compare physical properties among three wire groups; 0.016 X 0.016 wires (group 016), electroplated 0.016 x 0.016 wires (group 016P) and 0.017 x 0.017 wires (group 017). Through the investigation of each wire group, following results were obtained. 1. At three-point bending test, the group 016P showed higher tendency in the degree of stiffness, yield strength and ultimate tensile strength than the group 016. Stiffness and ultimate tensile strength showed statistically significant differences between two groups at three-point bending test (p<0.05). 2. Stiffness, yield strength, and ultimate tensile strength of the group 016P showed lower tendency than those of the group 017. Stiffness showed statistically significant differenced between two groups at three-point bending test (p<0.05). 3. Torque/twist rate, yield torsional moment, and ultimate torsional moment of the group 016P showed higher tendency than those of the group 016. All measurements showed statistically significant differences between two groups after torsion test (p<0.05). 4. Torque/twist rate, yield torsional moment, and ultimate torsional moment of the group 016P showed lower tendency than those of the group 017. Yield torsional moment, and ultimate torsional moment showed statistically significant differences between two groups after torsion test(p<0.05)

Fluoroquinolone계 항균제의 penicillin 내성 폐렴구균(Streptococcus pneumoniae)에 대한 항균력 비교

이광준,배송미,황규잠,이영희,김기상 대한화학요법학회 2002 대한화학요법학회지 Vol.20 No.3

목적 : 최근들어 penicillin 내성 폐렴구균의 급속한 증가와 전세계로의 확산은 심각한 문제를 야기하고 있다. 1990년대 들어 그람음성 세균뿐 아니라 그람 양성 세균에 대한 항균력이 향상된 많은 fluoroquinolones계 항균제의 개발에 힘입어 폐렴구균성 감염질환 치료시 사용이 증대되고 있으며 한편, 이와 더불어 이미 fluoroquinolone계 항균제에 대한 내성을 보이는 폐렴구균에 대한 보고가 증가하고 있는 실정이다. 이에 본 실험에서는 국내 임상 분리 폐렴구균 중 penicillin에 고도내성을 보이는 균주를 대상으로 하여 fluoroquinolones계 항균제에 대한 감수성 여부를 확인하고자 하였다. 방법 : 항균제 감수성 검사는 3% 말혈액이 첨가된 Mueller-Hinton broth를 사용한 액체배지 미량희석법을 이용하여 NCCLS에서 제시한 방법에 준하여 실시하였다. 결과 : MIC_90를 기준으로 하였을 때 본 실험에서 사용한 5가지의 fluoroquinolones계 한균제 중 gatifloxacin과 moxifloxacin(MIC_90, 0.5㎍/㎖)이 ciprofloxacin (MIC_90, 4㎍/㎖), levofloxacin (mic_90, 2㎍/㎖) sparfloxacin (MIC_90, 1㎍/㎖)에 비해 penicillin 내성 폐렴구균에 대한 항균력이 높게 나타났다. 결론 : 5가지의 fluoroquinolones계 항생제 중 최근들어 새로이 개발된 항생제인 moxifloxacin과 gatifloxacin이 ciprofloxacin, levofloxacin, sparfloxacin보다 폐렴구균에 대한 더 우수한 항균력을 가지고 있음을 확인하였다. 한편, 국내 페니실링 고도 내성 폐렴구균에서 fluoroquinolones에 대한 고도 내성을 보이는 균주의 출현은 fluoroquinolones 내성 균주의 급속한 증가와 확산에 대한 지속적인 감시와 대책 마련이 요구된다. Background : Recently the rapid increase and global spread of penicillin-resistant S. pneumoniae has become a serious problem. In the 1990s, a varisty of novel fluoroquinolones with enhanced activity against gram-positive bacteria have been developed and used for the treatment of pneumococcal infections. In such situations, the fluoroquinolone-resistance in S. pneumonaie has been reported from many countries. In this study, we compared the activity of ciprofloxacin, levofloxacin, sparfloxacin, gatifloxacin and moxifloxacin against 93 isolates of S. pneumoniae which were highly- resistant to penicillin (MIC 2~8㎍/㎖). methods : Antimicrobial susceptibilities were determined by the broth microdilution method in cation-adjusted Muller-Hinton broth supplemented with 3% lysed horse blood according to the guidelines of the National Committee for Laboratory Standards (NCCLS). Results : Based on MIC_90s, sparfloxacin and moxifloxacin were the most potent fluoroquinolone tested against penicillin-resistant S. pneumoniae and retained excellent activity comparable with ciprofloxacin and levofloxacin. The rank order of bactericidal activity was ciprofloxacin (least active)《 levofloxacin 〈 sparfloxacin 〈 gatifloxacin and moxifloxacin (most active). Conclusion : In this study, the newer fluoroquinolones (moxifloxacin, sparfloxacin)showed improved activity over that observed with ciprofloxacin, levofloxacin, and sparfloxacin. The emergence of highly resistance to fluoroquinolones among penicillin-resistant pneumococci in Korea call for continuous attention and strategies to prevent rapid increase and spread of these strains.

전기도금한 교정용 스테인레스스틸 선재의 세포독성에 관한 연구

이계형,조진형,이기헌,황현식 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.2

본 연구는 전기도금을 이용하여 교정용 스테인레스스틸 선재의 직경을 증가시킨 후 세포독성을 알아봄으로써 이의 임상적 적용 가능성을 알아보고자 시행되었다. 시판되고있는 교정용 스테인레스스틸 선재에 전기도금 방법을 이용하여 니켈을 도금한 후, 이를 도금하지 않은 교정용 스테인레스스틸 선재 그리고 티타늄 또는 구리와 비교하기 위하여 이들을 배지에 넣고 72시간 배양하였다. 이 배양액을 금속 유리량 측정을 위해 ICP-AES(inductively coupled plasma atomic emission spectrophotometer) 분석을 시행한 결과 전기도금한 선재에서 많은 양의 니켈 이온이 유리되었으며, 구리에서는 많은 양의 구리 이온이 유리되었다. Microplate에 Balb/c 3T3 mouse fibroblast를 넣고 각 선재를 배양한 배양액을 75%, 50%, 25%로희석시켜 첨가한 후 MTT (3-[4.5-dimethylthiazol-2yl]-2.5-diphenyl tetrazolium bromide)분석을 시행하여 이를 배지만을 첨가한 경우와 흡광도를 비교하였다. 티타늄은 모든 농도에서 배지만을 첨가한 경우에 비해 흡광도 차이를 보이지 않았지만전기도금한 선재(p<0.001), 스테인레스스틸선재(p<0.05), 구리(p<0.001)는 배지만을 첨가한 경우보다 통계적으로 유의한 흡광도 감소를 보였다. ISO 10993, part5에 따라 구리는 "강한 독성"을 보이는 반면 티타늄과 스테인레스스틸 선재는 "무독성", 그리고 전기도금 선재는 "중등도 독성"으로 나타났다. 이상의 결과는 전기도금을 이용하여 교정용 선재의 직경을 증가시키는 방법이 임상에 적용되기 위해서는 세포독성을 줄이기 위한 추가의 연구가 필요함을 시사하였다. The purpose of this study was to examine the cytotoxicity of orthodontic wire which had an increased diameter through electroplating, and to evaluate its possoble clinical applications. First nickel plating was carried out on the commercially available stainless steel wire using an electroplating technique. For the comparison of the electroplated wire with ready made stainless steelwire and titanium or copper, each wire was incybated for 72 hours in a medium. The release of the metal ion was measured using ICP-AES(Inductively Coupled Plasma Atomic Emission Spectrophotometer). Balb/c 3T3 mouse fibroblast was put on a microplate and placed in an incubated medium of 75%, 50%, and 20% dilation. An MTT analysis was used to compare with the medium only. The changein absorbency value of each wire group and the difference of absorbency value according to the change of dilution was measured. The results of ICP-AES analysis showed that great amount nickel ion was isolated from electrpolated orthodontic wires and great amount copper ion was isolated from copper.The results of the MTT analysis showed that there was no difference in the absorbency value of titanium at any dilution. However the electroplated wires(p<0.001), the stainless steel wires(p<0.05) and the copper(p<0.001) were statistically significantly lower than those of medium only at all dilutions. Assessment as per ISO 10993, part5, showed that electroplated wire was alloted to "moderate cytotoxic", the titanium and stainless steel wire were "non-cytotoxic". The results of this study indicate that the electroplated or thodontic wires need additional efforts to dectease cytotoxicity for their clinical applications.

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황선식,이기돈 湖西大學校工業技術硏究所 2003 工業技術硏究所論文集 Vol.22 No.-

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