전남지역 주민에서 마이코플라스마 폐렴에 대한 항체가 분포

배송미,장미정,전두영,권순석,강연호 대한임상미생물학회 2007 Annals of clinical microbiology Vol.10 No.2

Background: Mycoplasma pneumoniae is the most frequent cause of respiratory tract infections in schoolaged children and adolescents. For appropriate use of antibiotics, diagnosis of M. pneumoniae infection in routine clinical practice has been based on serology using a single serum sample. We evaluated the seroprevalence of anti-M. pneumoniae-specific antibodies in 500 asymptomatic, healthy persons in Jeonnam Province. Methods: Sera were collected from 500 healthy persons in Jeonnam Province. Anti-M. pneumoniae antibody titer was measured using a microparticle agglutination assay Serodia Myco II (Fujirebio, Japan) and VIRCELL IgM Mycoplasma ELISA kits (Vircell, Granada, Spain). Results: Anti-M. pneumoniae antibody titers in 500 healthy individuals were 1:20 in 344 (68.8%), 1:40 in 16 (3.2%), 1:80 in 71 (14.2%), 1:160 in 45 (9.0%), 1:320 in 14 (2.8%), and >1:320 in 10 (2.0%). The positive rate of M. pneumoniae IgM antibodies was 3.2% (15/473). The prevalence of IgM was 10.0% in the 7∼9 years, 9.1% in the 10∼19 years, and 5.0% in the 20∼29 years old group, which was significantly higher than that in elderly people. Conclusion: Some of healthy people showed a high anti-M. pneumoniae antibody titer (>1:160) and positive IgM, and an assessment of current infection with single serum serology has its limitation for the diagnosis of M. pneumoniae infections.

Session 1 : 국제적 이슈 인수공통전염병 현황 및 예방대책 ; S1-2 결핵 조기퇴치 New 2020 Plan을 위한 전략

배송미 대한인수공통전염병학회 2012 창립총회 및 학술대회 초록집 Vol.2012 No.1

Synthesis of Idebenonyl Ester Prodrugs and Their Evaluation of Cancer Cells In Vitro

배송미,최세명,원영선,서권일,정대일,송주현,조종현 대한화학회 2021 Bulletin of the Korean Chemical Society Vol.42 No.4

Esterification of idebenone with commercially available straight-chain aliphatic acids, branched-chain aliphatic acids, aromatic acids, or nonsteroidal anti-inflammatory drugs afforded their corresponding idebenonyl esters (5a–k) in good yield. The values of cell viability for RC-58T cell at 50 μM were 86.3% for 5a, 75.9% for 5b, 23.4% for 5d, 61.2% for 5f, 67.9% for 5h, 70.6% for 5i, and 49.0% for 5j.

2001년 국내 해양환경에서 분리된 Vibrio vulnificus의 생화학적 특성 및 항생제 감수성 패턴

황규잠,주영인,이영희,이광준,배송미,김기상 대한미생물학회 2003 Journal of Bacteriology and Virology Vol.33 No.4

Fluoroquinolone계 항균제의 penicillin 내성 폐렴구균(Streptococcus pneumoniae)에 대한 항균력 비교

이광준,배송미,황규잠,이영희,김기상 대한화학요법학회 2002 대한화학요법학회지 Vol.20 No.3

목적 : 최근들어 penicillin 내성 폐렴구균의 급속한 증가와 전세계로의 확산은 심각한 문제를 야기하고 있다. 1990년대 들어 그람음성 세균뿐 아니라 그람 양성 세균에 대한 항균력이 향상된 많은 fluoroquinolones계 항균제의 개발에 힘입어 폐렴구균성 감염질환 치료시 사용이 증대되고 있으며 한편, 이와 더불어 이미 fluoroquinolone계 항균제에 대한 내성을 보이는 폐렴구균에 대한 보고가 증가하고 있는 실정이다. 이에 본 실험에서는 국내 임상 분리 폐렴구균 중 penicillin에 고도내성을 보이는 균주를 대상으로 하여 fluoroquinolones계 항균제에 대한 감수성 여부를 확인하고자 하였다. 방법 : 항균제 감수성 검사는 3% 말혈액이 첨가된 Mueller-Hinton broth를 사용한 액체배지 미량희석법을 이용하여 NCCLS에서 제시한 방법에 준하여 실시하였다. 결과 : MIC_90를 기준으로 하였을 때 본 실험에서 사용한 5가지의 fluoroquinolones계 한균제 중 gatifloxacin과 moxifloxacin(MIC_90, 0.5㎍/㎖)이 ciprofloxacin (MIC_90, 4㎍/㎖), levofloxacin (mic_90, 2㎍/㎖) sparfloxacin (MIC_90, 1㎍/㎖)에 비해 penicillin 내성 폐렴구균에 대한 항균력이 높게 나타났다. 결론 : 5가지의 fluoroquinolones계 항생제 중 최근들어 새로이 개발된 항생제인 moxifloxacin과 gatifloxacin이 ciprofloxacin, levofloxacin, sparfloxacin보다 폐렴구균에 대한 더 우수한 항균력을 가지고 있음을 확인하였다. 한편, 국내 페니실링 고도 내성 폐렴구균에서 fluoroquinolones에 대한 고도 내성을 보이는 균주의 출현은 fluoroquinolones 내성 균주의 급속한 증가와 확산에 대한 지속적인 감시와 대책 마련이 요구된다. Background : Recently the rapid increase and global spread of penicillin-resistant S. pneumoniae has become a serious problem. In the 1990s, a varisty of novel fluoroquinolones with enhanced activity against gram-positive bacteria have been developed and used for the treatment of pneumococcal infections. In such situations, the fluoroquinolone-resistance in S. pneumonaie has been reported from many countries. In this study, we compared the activity of ciprofloxacin, levofloxacin, sparfloxacin, gatifloxacin and moxifloxacin against 93 isolates of S. pneumoniae which were highly- resistant to penicillin (MIC 2~8㎍/㎖). methods : Antimicrobial susceptibilities were determined by the broth microdilution method in cation-adjusted Muller-Hinton broth supplemented with 3% lysed horse blood according to the guidelines of the National Committee for Laboratory Standards (NCCLS). Results : Based on MIC_90s, sparfloxacin and moxifloxacin were the most potent fluoroquinolone tested against penicillin-resistant S. pneumoniae and retained excellent activity comparable with ciprofloxacin and levofloxacin. The rank order of bactericidal activity was ciprofloxacin (least active)《 levofloxacin 〈 sparfloxacin 〈 gatifloxacin and moxifloxacin (most active). Conclusion : In this study, the newer fluoroquinolones (moxifloxacin, sparfloxacin)showed improved activity over that observed with ciprofloxacin, levofloxacin, and sparfloxacin. The emergence of highly resistance to fluoroquinolones among penicillin-resistant pneumococci in Korea call for continuous attention and strategies to prevent rapid increase and spread of these strains.

국내 임상 분리주 Streptococcus pneumoniae KNIH1156으로부터 PspA 단백 항원의 정제 및 면역원성 확인

정경석,배송미 한국미생물학회 2002 미생물학회지 Vol.38 No.1

국내 분리 침습성 균주중선별된 S. pneumoniae KNIH1156 (type 19F)으로부터 페렴구균의 병원성 인자이며 항원학적으로 다양한 표면단백항원인 pneumococcal surface protein A (PspA)를 분리${\cdot}$정제하였다. 폐렴구균을 CDM-ET배지에서 배양하게 되면 배지내로 PspA가 방출된다는 점과 PspA가 인간의 lactoferrin에 특이적으로 결합한다는 사실을 이용하여 CDM-ET 배지에서 S. pneumoniae KNIH1156 을 배양한 후 배양액을 농축하여 lactoferrinaffinity chromatography에 통과시켜 PspA를 분리, 정제하였다. 정제 후 anti-PspA antiserum으로 PspA를 확인하여 순수분리, 정제되었음을 확인하였으며 또한 인간의 lactoferrin과의 결합능력을 유지하고 있음을 확인하였다. 순수하게 분리하여 정제된 PspA의 면역원성을 확인하기 위하여 ICR mice에 욕강주사하였을 때 $LD_{50}$ 가 $1{\times}10^{7.5}$ CFU/ml께서 $1{\times}10^{10}$ CFU/ml로 약 500배 중가함을 관찰하였다. 따라서 본 실험에서S. pneumoniae KNIH1156 으로부터 분리${\cdot}$ 정제한 PspA가 면역원성과 방어능을 가지고 있음을 확인할 수 있었다. Pneumococcal surfacce protein A (PspA) is an important virulence factor and an antigenically variable surface protein of the pneumococci. To purify the PspA from S. pneumoniae KNIH1156 , a clinical isolate (type 19F), we have taken advantage of the fact that PspA is released from the surface of pneumococci into the medium by growing in a CDM-ET medium and PspA is capable of binding human lactoferrin, the iron carrier protein. PspA of S. pneumoniae KNIH1156 was purified from culture supernatant by human lactoferrin (hLf) affinity chromatography. The purified PspA was confirmed with anti-PspA antiserum and also had the binding capacity to hLf specifically. To determine whether the purified PspA could elicit protection in mice against pneumococcal inflection, we immunized the mice with purified PspA and subsequently challenged with S. pneumoniae KNIH1156. Immunization with purified PspA protected mice from 500 times the $LD^{50}$ of S. pneumoniae KNIH1156. Therefore, it has been shown that purified PspA fromS. pneumoniae KNIH1156 (type 19F) is a protective immunogen.

Cluster of Serogroup W-135 Meningococcal Disease in 3 Military Recruits

조유미,배송미,강연호 대한의학회 2015 Journal of Korean medical science Vol.30 No.5

We describe a group of 3 cases of invasive meningococcal disease that occurred in a military training camp in April 2011. All three patients were hospitalized. Ultimately, two patients recovered and one died. One patient had meningitis, one patient had septicemia and meningitis, and the other had no definite septicemia or meningitis. Neisseria meningitidis serogroup W-135 was detected in the serum and cerebrospinal fluid (CSF) of all patients by real-time polymerase chain reaction. In the one case of mortality, two strains were isolated from the patient’s blood and CSF. Using multilocus sequence typing analysis, these strains were identified as a novel sequence type, ST-8912. Special attention is required for the meningococcal disease in military camp because the military personnels are in high risk of contact transmission.

Synthesis and Antiepileptic Activity Evaluation of Valproic Acid Derivatives by Niche Chemistry

송주현,배송미,Whae Ran Bae,Jin Sun Huh,Yu Chen,Il Soo Jeong,정대일 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.5

In order to prepare new antiepileptic drugs with a broader clinical application and lower toxic side effects, we have employed esterification and condensation reactions (we call it Niche Chemistry) to reduce the use of valproic acid as the target molecule. In combination with the study of a broad-spectrum of novel anticonvulsants, we have synthesized two valeric acid derivative compounds [(4-acetamidophenyl 2-propylvalerate (1), and (E)-5-(4-((2-propylpentanoyl)oxy)styryl)-1,3-phenylene bis(2-propylvalerate) (2) by an esterification reaction. The maximum shock seizure test for products 1 and 2 has been successfully carried out, and their cytotoxic activity and survival effects have also been carefully investigated.

한국 훈련병에서 수막염균 보균에 대한 4가 수막염균 결합 백신의 영향

허중연 ( Jung Yeon Heo ),배송미 ( Song-mee Bae ),정희진 ( Hee Jin Cheong ),김우주 ( Woo Joo Kim ),김민영 ( Min Young Kim ),나원웅 ( Wonwoong Na ),최강원 ( Kang-won Choe ),천병철 ( Byung Chul Chun ) 국군의무사령부 2014 대한군진의학학술지 Vol.45 No.1

Background: Monovalent meningococcal conjugate vaccines against serogroup A or C had resulted in significant reduction of pharyngeal carriage rates against respective vaccine serogroup. However, the effect of quadrivalent meningococcal conjugate vaccine on meningococcal carriage has not been evaluated. We assessed on changing pharyngeal carriage rate of Neisseria meningitidis after the introduction of quadrivalent meningococcal conjugate vaccine in Korean military trainees. Methods: Twice oropharyngeal swabs were conducted at the beginning and end of army training period in Korean military trainees. First oropharyngeal swabs were undertaken prior to vaccination. Second round of swabbing was done 5 weeks later. Results: In 2013, overall carriage rate of N. meningitidis was higher at the end of training period (13.8%, 60 of 434 participants) than on entry of military service (8.9%, 39 of 434 participants). Although carriage rate of nongroupable and serogroup X isolates were increased over 5-week interval, carriage strains of vaccine serogroup, which were included serogroups in quadrivalent meningococcal vaccine, were relatively reduced from 7 to 2 isolates following vaccination. However, in 2014, overall carriage rate of N. meningitidis was significantly lower at the end of training period (5.2%, 23 of 443 participants) than on entry of military service (12.6%, 56 of 443 participants) (p=0.028). Carriage isolates of vaccine serogroups were significantly decreased with that of non-vaccine serogroup B over 5-week interval (p=0.006). Conclusion: Meningococcal vaccination in Korean military trainees resulted in reduction of pharyngeal carriage rates of not only vaccine serogroup meningococci but non-vaccine serogroup B. Quadrivalent meningococcal conjugate vaccine is likely to preventing carriage acquisition of meningococci in a closed population.

Carriage Rates and Serogroups of Neisseria meningitidis among Freshmen in a University Dormitory in Korea

Areum Durey,이진수,배송미,이혜진,나소윤,김미정,백지현,강연호,정문현 연세대학교의과대학 2012 Yonsei medical journal Vol.53 No.4

Purpose: Neisseria meningitidis is a leading cause of bacterial meningitis in young adults. University students, especially those living in dormitories, have been known to be at increased risk of meningococcal disease. We performed a longitudinal study to determine the carriage rates of N. meningitidis and the changes thereof. Materials and Methods: We recruited Inha University freshmen who were, at that time, admitted to a student dormitory. A pharyngeal swab was taken from all participant who were also asked to complete a questionnaire. This was repeated four weeks later. Results: A total of 136 students were enrolled at the first culture. After four weeks, 128 students were enrolled, including 106 re-participants. The overall carriage rates changed from 11.8% to 14.1%. In analysis of the 106 re-participants, “visiting to pubs” was associated with carriage of N. meningitis for both the first (p=0.047) and second cultures (p=0.026). Serogroup C was found to be the most frequent serogroup (5 isolates), while 3 isolates were found from serogroup B. The most prevalent PorA types were P1.22,14-6 (4 isolates) and P1.19,15 (3 isolates). The DNA sequences of PorA VR2 were changed in 2 students during prolonged carriage. Conclusion: The meningococcal carriage rate among first year university students who resided in a dormitory did not significantly increase over 4-week interval between cultures, which is markedly different from those reported in Western studies. Close social contact appeared to be related with carriage. Our data also revealed diversity in PorA types, suggesting the possibility of rapid mutation of the PorA gene during the 4-week interval. Purpose: Neisseria meningitidis is a leading cause of bacterial meningitis in young adults. University students, especially those living in dormitories, have been known to be at increased risk of meningococcal disease. We performed a longitudinal study to determine the carriage rates of N. meningitidis and the changes thereof. Materials and Methods: We recruited Inha University freshmen who were, at that time, admitted to a student dormitory. A pharyngeal swab was taken from all participant who were also asked to complete a questionnaire. This was repeated four weeks later. Results: A total of 136 students were enrolled at the first culture. After four weeks, 128 students were enrolled, including 106 re-participants. The overall carriage rates changed from 11.8% to 14.1%. In analysis of the 106 re-participants, “visiting to pubs” was associated with carriage of N. meningitis for both the first (p=0.047) and second cultures (p=0.026). Serogroup C was found to be the most frequent serogroup (5 isolates), while 3 isolates were found from serogroup B. The most prevalent PorA types were P1.22,14-6 (4 isolates) and P1.19,15 (3 isolates). The DNA sequences of PorA VR2 were changed in 2 students during prolonged carriage. Conclusion: The meningococcal carriage rate among first year university students who resided in a dormitory did not significantly increase over 4-week interval between cultures, which is markedly different from those reported in Western studies. Close social contact appeared to be related with carriage. Our data also revealed diversity in PorA types, suggesting the possibility of rapid mutation of the PorA gene during the 4-week interval.