Benzyl Isothiocyanate Inhibits Prostate Cancer Development in the Transgenic Adenocarcinoma Mouse Prostate (TRAMP) Model, Which Is Associated with the Induction of Cell Cycle G1 Arrest

Cho, Han Jin,Lim, Do Young,Kwon, Gyoo Taik,Kim, Ji Hee,Huang, Zunnan,Song, Hyerim,Oh, Yoon Sin,Kang, Young-Hee,Lee, Ki Won,Dong, Zigang,Park, Jung Han Yoon MDPI 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.2

<P>Benzyl isothiocyanate (BITC) is a hydrolysis product of glucotropaeolin, a compound found in cruciferous vegetables, and has been shown to have anti-tumor properties. In the present study, we investigated whether BITC inhibits the development of prostate cancer in the transgenic adenocarcinoma mouse prostate (TRAMP) mice. Five-week old, male TRAMP mice and their nontransgenic littermates were gavage-fed with 0, 5, or 10 mg/kg of BITC every day for 19 weeks. The weight of the genitourinary tract increased markedly in TRAMP mice and this increase was suppressed significantly by BITC feeding. H and E staining of the dorsolateral lobes of the prostate demonstrated that well-differentiated carcinoma (WDC) was a predominant feature in the TRAMP mice. The number of lobes with WDC was reduced by BITC feeding while that of lobes with prostatic intraepithelial neoplasia was increased. BITC feeding reduced the number of cells expressing Ki67 (a proliferation marker), cyclin A, cyclin D1, and cyclin-dependent kinase (CDK)2 in the prostatic tissue. <I>In vitro</I> cell culture results revealed that BITC decreased DNA synthesis, as well as CDK2 and CDK4 activity in TRAMP-C2 mouse prostate cancer cells. These results indicate that inhibition of cell cycle progression contributes to the inhibition of prostate cancer development in TRAMP mice treated with BITC.</P>

각종 난치성 혈액 질환에서의 비혈연간 골수이식

김동욱,한훈,김정아,김희제,민창기,엄현석,최정현,이종욱,한치화,홍영선,최일봉,신완식,민우성,김학기,김춘추,김원일,김동집 대한조혈모세포이식학회 1997 대한조혈모세포이식학회지 Vol.2 No.1

목적: 비혈연간 골수이식은 혈연내에 적절한 골수공여자가 없는 만성골수성백혈병, 고위험군의 급성별혁병, 면역억제치료에 실패한 재생불량성빈혈 및 각종 난치성 조혈모세포질환의 완치를 위한 표준적인 치료방법으로 정착되고 있다. 혈연간 표준적인 동종 이식에 비하여 비혈연간 이식시에는 생착부전, 이식편대숙주반응과 감염이 더 빈번하게 발생하며, 국내에서는 아직까지 체계적인 임상연구결과가 보고된 바 없었다. 이에 본 센터에서는 1995년 10월 이후로 약 20개월간 26예의 비혈연간 골수이식을 시행하였으며 3개월 이상의 추적관찰이 가능하여 이식초기 합병증의 관찰 및 분석이 가능하였던 20예의 환자를 대상으로 이식성적 및 문제점을 보고함으로써 새롭게 확대되고 있는 이 분야의 임상연구 및 진료의 활성화를 꾀하고자 한다. 방법: 각종 혈액 종양질환으로 비혈연간 이식을 시행한 총 26예의 환자중 3개월이상의 추적관찰이 가능하였던 20예를 대상으로 후향적으로 임상경과를 분석한 후 생존 분석을 시행하였고, 환자의 연령, 성별, 질병의 상태, 조식 적합 항원의 일치정도, 이식편대 숙주 반응의 유무와 생존기간과의 상관관계를 살펴보았다. 또한 표준위험군과 고위험군으로 나누어 생존율을 비교하였고 이식과 관계된 생착 부전, 이식편대숙주반응, 감염의 발생과 양상 그리고 그 합병증을 관찰하였다. 결과: 1. 환자와 공여자간에 HLA 불일치가 20예 중 4예에서 있었으며, 생착여부의 확인이 가능했던 17예 중 16예에서 생착이 확인되어 94.1%의 생착율을 보였다. 2. 급성이식편대숙주반응은 62.5%(10/16예)에서 발생하였으며 111도 이상의 급성의 이식편대숙주 반응은 25%(4/16예)에서 발생하였다. 만성이식편대숙주반응은 40%(2/5예)의 환자에서 발생하였으며 이들 모두 국소형으로 중증의 진행형 만성이식편대숙주반응이 관찰된 환자는 없었다. 3. 호흡기 합병증은 10예(50%)에서 발생하였으며 감염성 폐렴을 포함한 호흡기 합병증이 가장 흔한 일차적인 사망 원인이었다. 호흡기 합병증이 발생했던 10예중 6예가 감염에 의한 폐렴이 의심되었고 나머지 4예는 특발성 간질성 폐렴이었다. 4. 8.5개월의 중앙추적기간 중 35%의 생존율을 관찰할 수 있었고, 생존기간은0.5개월에서 15개월 (중앙치:4개월)이었다. 한편 고위험군은 25%(3/12예), 표준위험군은 50%(4/8)의 생존율을 관찰할 수 있었다. 5. 가장 흔한 사망 원인은 감염성 폐렴을 포함한 호흡기 합병증(6예)이었고, 이외의 사망 원인으로는 급성 이식편대숙주반응과 다장기부전이 각각 2예, 생착 부전, 간정맥 폐쇄, 그리고 재발이 각각 1예였다. Unrelated bone marrow transplantation(UBMT) has been increasingly recognized as the standard treatment for cure of chronic myelogenous leukemia, high risk acute leukemia, aplastic failed on immunotherapy, and the variety of refractory hematologic diseases in patients lacking a related donor. However, as compared to HLA identical sibing transplantation, UBMT carries higher incidence of graft failure, graft versus host disease(GVHD), and infection. In our center, 26 patients underwent UMBT between October 1995 and June 1997. The minimum follow-up of 3 months was possible in 20 patients, for whom early complications and clinical outcomes were assessed. The median age of the 20 patients was 24 years. 8 patients had standard risk disease and 12 patients had high risk disease. All patients received various preparative regimens including total body irradiation according to disease and disease status. 19 patients received CsA + short course MTX for GVHD prophylaxis. One patient received marrow that was depleted of T cells ex vivo using avidinbiotin column. The class I loci were typed by serological methods and HLA-A, HLA-B and HLA-DRB1. 3 additional pairs were one minor mismatched at the HLA-B locus. Another one patients was one major mismatched at the DRBI alleles. 17 patients were evaluable for engraftment. Successful enfraftment was confirmed in 16 patients(94.1%). Only one patient who was performed one major DRBI mismatched transplants experienced graft rejection. 16 patients were evaluable for acute GVHD. The overall incidence of acute GVHD developed in 4 patients(25%). Five patients were evaluable for the development of Ⅳ acute GVHD developed in 4 patients (25%). Five patients were evaluanle for the development of chronic GVHD. 2 patients(40%) developed limited chronic GVHD. Respiratory complications including pulmonary infection developed in 10 patients(50%) and these complications were the most common primary cause of death. Of these 10patients, 6 had pneumonia due to fungus(4 patients), pacterial (1 patient), and CMV infection (1 patient) and 4(20%) had idiopathic interstitial pneumonitis and/or adult respiratory distress syndrome. The duration of median follow- up was 8.5 months and 7 of 20 patients(35%) are alive at the time of this analysis with survival duration of 0.5 to 15 months(median survival duration: 4 months). The overall survival was 25% (3/12 patients) in high risk group and 50%(4/8 patients) in standard group. From these results, we can predict that the incidence and severity of GVHD in Korea are lesser than multiracial countries and the long-term survival of patients with standard risk disease can approach that of HLA matched sibling transplants. For the past two years, the performance of UBMT has been rapidly increasing and it will be possible to analyze much larger number of patients soon in Korea. In the future the problems of graft failure, GVHD, and infection due to long lasting immunocompromised status will need to be overcome by continued medical research. In addition, the volunteer donor pool will have to be expanded by the promotion of the national awareness of its need.

장애인생활체육지도자의 코칭행동 구성 요인 분석

한동기 ( Dong-ki¸ Han ) 한국특수체육학회 2020 한국특수체육학회지 Vol.28 No.4

본 연구의 목적은 장애인생활체육지도자의 코칭행동 구성 요인을 분석하는 것이었다. 이 목적을 달성하기 위해 시도 및 시군구 장애인체육회에 근무하는 장애인생활체육지도자 205명을 연구대상자로 선정하였다. 개방형 설문지를 사용하여 장애인 생활체육 지도의 코칭행동 3가지를 기술하도록 하였으며, 설문 결과 총 603개 반응이 나타났다. 설문 응답 내용은 귀납적 내용분석(inductive content analysis)을 사용하여 분석하였으며, 그 결과는 다음과 같다. 대영역으로 장애와 프로그램 이해(지식), 체육활동 지도와 실행(기술), 바람직한 인성과 태도(태도) 등 3개 요인이 도출되었다. 중영역으로는 대상자 파악, 체육프로그램 개발 및 계획, 전문적지도 능력, 개별화 교육, 지도 외적 요인, 관계 형성, 적극적 태도 등 7개 요인, 소영역으로는 대상자 특성 파악, 개별화된 지도, 소통 등 31개의 요인이 도출되었다. 장애인생활체육지도자들은 체육활동 지도와 실행(기술)을 가장 중요한 코칭행동 요인으로 생각하고 있었으며, 바람직한 인성과 태도(태도)도 코칭행동의 주된 요인으로 인식하고 있었다. 추후 연구에서는 장애인체육 전문가와 생활체육에 참여하는 장애인들이 생각하는 코칭행동 구성 요인을 파악해 볼 필요가 있을 것이다. The purpose of this study was to analyze the factors that constitute the coaching behaviors of the sports leaders for the disabled. To achieve this goal, 205 sports leaders for the disabled who work at the city, county, and district sports associations for the disabled were selected as subjects for the study. An open questionnaire was used to describe the three coaching behaviors of the physical activities for the disabled, and a total of 603 responses were found as a result of the questionnaire. The contents of the survey responses were analyzed using inductive content analysis, and the results are as follows. Three factors were derived: understanding of disability and programs (knowledge), guidance and execution of physical activities (skills), and desirable personality and attitude (attitude) as major areas. In the middle area, 7 factors including subject characteristics identification of participants, physical activity program development and planning, professional guidance ability, individualized education, factors outside of guidance, relationship formation, active attitude, etc., in small areas, 31 factors such as subject characteristics identification, individualized guidance, and communication was derived. It was found that the sports leaders for the disabled regarded physical activity guidance and execution (skill) as the most important factor, and desirable personality and attitude (attitude) were also recognized as the main factors of coaching behavior in physical activity guidance. In future studies, you may need to identify the components of coaching behaviors that are thought of by professionals in disability sports and those with disabilities participating in life sports.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

물체조작 활동이 다운증후군 아동의 눈-손 협응력에 미치는 영향

한동기,한민정 한국특수체육학회 2004 한국특수체육학회지 Vol.12 No.2

The purpose of this study was to investigate the effectiveness of object control activities on the eye-hand coordination and object control skill ability of children with Down syndrome. As the subject of this study, elementary school boys(n=16) with trisomy 21 Down syndrome were selected. The experiment was conducted for 16 weeks, 2 sessions per week, and 90 minutes per session. In addition, a test on eye-hand coordination and a test on five object control skills((rolling underhand, overhand throw, catch, two-hand strike, stationary bounce) were conducted before and after the program effectuated. The data were analyzed by ANCOVA for identifying difference between the experiment group and control group. The significant level was set at a=.05 to verify the hypothesis. The results of this study were drawn as follows: It could be seen that after the object control activities was effectuated for 16 weeks, there was statistically significant positively effected the enhancement of eye-hand coordination and object control skill ability of children with Down syndrome. In conclusion, the object control activities positively effected the enhancement of eye-hand coordination and object control skill ability of children with Down syndrome.

정신지체 특수학교 초등교사의 체육목표 인식 분석

한동기,김권일 한국특수체육학회 2003 한국특수체육학회지 Vol.11 No.2

The purpose of this study was to analyze special teachers' recognition toward the goals of physical education in the elementary school for the students with mental retardation. The subjects of this study were 237 teachers in the elementary school for the students with mental retardation, located in Seoul, Kyong-Ki, and In-Chon. The results of this study were as follows: 1. The male teacher group showed a higher than female teacher group recognition level on the goals except for the goals of psychic equilibrium and spatial orientation. 2. There were differences in the goals of physiological efficiency, object manipulation, communication, group interaction and cultural involvement among the groups by career. 3. Teachers of lower grade(1~3) have a higher level of recognition than level of upper grade(4~6) on the goal of 6 areas except the goal of spatial orientation. 4. There were no differences in recognition level of the goals of the physical education between the teachers with physical education class responsible and without.

니트섬유강화 복합재료의 기계적 성질에 관한 연구

한길영,이동기,최병기,오환교,신용욱,전수영 조선대학교 에너지.자원신기술연구소 1999 에너지·자원신기술연구소 논문지 Vol.21 No.2

Using conventional textile techniques such as weaving, braiding, knitting and stitching it is possible to produce a wide range two and three dimensional fiber preforms. However, so far only a limited attention has been given to knitted fabrics in composite industry. This is mainly due to the opinion that knitted fabric reinforced composites posses low mechanical properties owing to their looped fiber architecture. But it is possible to obtain desired mechanical properties by selecting proper knitted fabric structure. In this paper, tensile failure load and displacement of one yarn, four yarns and knitted fabrics of ten yarns of kevlar fiber were determined experimentally in the unidirection. Mechanical properties of plain weft knitted fabrics reinforced composite intrusion beam for car side door application were investigated experimentally, which compared with desire value of American Fedral Motor vehicles Safety Standard (FMVSS) and presented the results.

다운증후근 아동과 청소년의 슬관절 등속성 근력 특성 비교

한동기,김권일 한국특수체육학회 2003 한국특수체육학회지 Vol.11 No.1

This study was to provide fundamental information of muscle strength training, gait, and gross motor movement through comparing and analyzing low limb isokinetic torque of children and adolescents with Down syndrome. The subjects were 8 children with Down syndrome and 8 adolescents with Down syndrome. The peak torque, average power, and total work were measured with Cybex 770 at the speed 0f 60˚/sec and 180˚/sec. The data were analyzed by SPSS. The conclusions were drawn as follows; 1. The peak torque was decreased as angular velocity increasing. 2. Muscle strength was unequal between left and right knee joint. 3. Adolescents with Down syndrome were higher that children in the peak torque, average power, total work, and flexor/extensor ratio. Therefore, children and adolescents with Down syndrome had normal characteristics related with isokinetic strength. However, there are lots of possibilities of injury during activities because of the imbalance between left and right leg and low flexor/extensor ratio.

한국 고대 제천의례의 스포츠과학적 고찰

한상준,박기동 江原大學校附設 體育科學硏究所 1991 江原大學校附設體育科學硏究所論文集 Vol.- No.16

Korean ancient Checheon Ceremony(祭天儀禮) is examined and recognized the theory that sport is originated from amunement, as forementioned. It is because of human general liking nature for play that have continued until now. In this sense, the relationships btn play and festiveal, btn play and sport, and btn play and labour are examined. As the result, play belonged tho festival should be revived in relation to today's sport. Sport can be existed beyond selfish mind of both individual and group, and beyond historical viewpoint. In spite of all threats and obtacles corresponding that, sport can become one of the most far-reaching and important thing among the every human cultural attitudes.

Ethyl Cellulose를 이용한 요소비료의 방출에 관한 연구

한성철,이기영,김동운,송원현 全南大學校 觸媒硏究所 2002 觸媒硏究 論文集 Vol.23 No.-

Release study of urea fertilizer was performed using ethyl cellulose and rosin gum. Various effect on release kinetic was examind such as concentration of ethyl cellulose, types of emulsifier, and ethyl cellulose : rosin. Sustained release effect was optimal when 15%(w/v) ethyl cellulose, span 85, ethyl cellulose : rosin gum = 5 : 2 were used.